##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293612
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336039
Total Bases	57.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.45332535806856	39.0	39.0	39.0	37.0	39.0
2	40.2230574427373	41.0	40.0	41.0	38.0	41.0
3	40.25410443430673	41.0	40.0	41.0	38.0	41.0
4	40.26047274274712	41.0	40.0	41.0	38.0	41.0
5	40.26441276161398	41.0	40.0	41.0	39.0	41.0
6	40.242415314889044	41.0	40.0	41.0	38.0	41.0
7	40.26002934183235	41.0	40.0	41.0	39.0	41.0
8	40.24325450319755	41.0	40.0	41.0	39.0	41.0
9	40.21274316373992	41.0	40.0	41.0	38.0	41.0
10-14	40.17175030279223	41.0	40.0	41.0	38.0	41.0
15-19	40.03164513642762	41.0	40.0	41.0	38.0	41.0
20-24	39.82813721026429	41.0	40.0	41.0	37.8	41.0
25-29	39.59061358949408	40.4	39.2	41.0	36.8	41.0
30-34	39.37304062921268	40.0	39.0	41.0	35.8	41.0
35-39	39.271742863179576	40.4	38.4	41.0	35.0	41.0
40-44	38.813641273780725	40.0	37.0	41.0	35.0	41.0
45-49	38.115067001151644	39.0	35.4	41.0	35.0	41.0
50-54	37.38985832001642	37.2	35.0	40.4	34.6	41.0
55-59	36.67803022863417	36.0	35.0	39.0	34.0	41.0
60-64	35.91597760974173	35.0	34.8	37.4	33.6	40.0
65-69	35.56117355426007	35.0	35.0	36.4	33.6	39.0
70-74	35.32184716654912	35.0	35.0	36.0	34.0	37.6
75-79	35.13503908772494	35.0	35.0	35.0	34.0	37.0
80-84	35.06112564315451	35.0	35.0	35.0	33.0	36.4
85-89	34.85130356893099	35.0	34.6	35.0	32.6	36.6
90-94	39.647581681335005	40.6	39.8	40.6	37.8	40.6
95-99	39.94682495253717	41.0	40.0	41.0	38.0	41.0
100-104	39.81438093696681	41.0	40.0	41.0	37.0	41.0
105-109	39.6540897861622	41.0	39.8	41.0	36.6	41.0
110-114	39.44156228254413	40.4	39.0	41.0	35.6	41.0
115-119	39.12739858770533	40.0	38.2	41.0	35.0	41.0
120-124	38.84396399148635	40.0	38.0	41.0	35.0	41.0
125-129	38.266490112836046	39.6	36.6	40.6	34.4	41.0
130-134	37.920426480108425	39.0	35.2	41.0	34.0	41.0
135-139	37.5427634175464	37.8	35.0	40.8	34.0	41.0
140-144	36.91432503198657	36.4	35.0	39.4	34.0	41.0
145-149	36.209331153243085	35.2	35.0	38.0	34.0	40.6
150-154	35.57299800427199	35.0	35.0	36.6	33.4	39.0
155-159	35.07572250482384	35.0	35.0	35.8	33.0	37.0
160-164	34.76801550830311	35.0	35.0	35.0	33.0	36.2
165-169	34.58114455705005	35.0	35.0	35.0	33.0	36.0
170-174	34.47876020059415	35.0	34.4	35.0	33.0	35.6
175-178	34.16842501309088	34.75	33.75	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	7.0
30	42.0
31	235.0
32	856.0
33	2541.0
34	6614.0
35	17498.0
36	51926.0
37	113387.0
38	114384.0
39	28404.0
40	144.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.633500873410526	26.70939980180872	29.13560628379444	22.521493040986314
2	23.080356744306467	27.719699201580767	27.853017060519765	21.346926993593005
3	20.097369650546515	26.351703224923295	30.474438978809008	23.07648814572118
4	21.478161760986076	25.312835712521466	30.505982936504395	22.703019589988067
5	22.693794470284697	26.096970887307723	27.977407384261944	23.231827258145632
6	22.540836033912733	26.66595246385092	27.798559095819236	22.994652406417114
7	22.936325843131304	26.778141822824136	27.12958912507179	23.15594320897277
8	23.018756751448493	27.070369808266303	26.679641351152693	23.23123208913251
9	23.02500602608626	27.23939780799253	26.952228759161883	22.783367406759332
10-14	23.07208389502409	27.069060436437436	26.730766369379744	23.128089299158727
15-19	22.950371831840947	27.146789509550974	26.919434946538946	22.983403712069133
20-24	22.90436526712673	26.937468567636497	27.05495493082648	23.103211234410292
25-29	22.99518805852892	26.815220852341543	26.88307011983728	23.306520969292254
30-34	23.017744964126187	26.961572912667876	26.80653138474998	23.214150738455952
35-39	23.096009689351536	26.857537369174413	26.80147244813846	23.244980493335596
40-44	23.147432292085146	26.83783727484012	26.78260559042254	23.23212484265219
45-49	23.154871904749154	26.809150128407715	26.773975639732296	23.262002327110842
50-54	23.219507259574037	26.885272245185828	26.700293715907975	23.19492677933216
55-59	23.167906106136492	26.769988007344388	26.78403399605403	23.278071890465093
60-64	23.14701567377596	26.91931591273632	26.75302569047045	23.180642723017268
65-69	23.106008528771955	26.861465484661007	26.83503998047846	23.19748600608858
70-74	23.163620889242022	26.804924428414562	26.739753421477864	23.291701260865555
75-79	23.08107094712221	26.83974181568211	26.83152848330105	23.247658753894637
80-84	23.117019155514686	26.881284612797916	26.721957867985562	23.27973836370183
85-89	23.056252399275085	26.842598626945087	26.754632646805877	23.34651632697395
90-94	21.903509033006934	26.567397839221407	28.904312628352034	22.624780499419625
95-99	22.835329566047243	27.023724847527376	27.136016660888256	23.004928925537126
100-104	22.91226728134247	27.134006445503513	26.92092652472079	23.032799748433227
105-109	22.88105281038934	27.0667301485524	27.022169214315962	23.030047826742305
110-114	22.861858782631234	26.92337461068115	27.086572032161893	23.128194574525722
115-119	22.901255197372183	27.047651736170696	26.86321088459952	23.187882181857596
120-124	22.908474580370676	27.00278936570199	26.816609938075235	23.2721261158521
125-129	22.91341137131873	26.960102938210344	26.929909187891393	23.19657650257953
130-134	23.019902369422766	27.015401980952753	26.76240782999184	23.202287819632637
135-139	23.142961678848014	26.802343597357233	26.7696138764061	23.285080847388652
140-144	23.116463242062103	26.935423418094295	26.822152416745492	23.125960923098106
145-149	22.98530339561196	27.074514454453354	26.85900003403204	23.08118211590265
150-154	23.003549215928807	27.04059726964754	26.802973427315102	23.152880087108553
155-159	22.940714936254036	27.0671172850887	26.919206131589636	23.07296164706763
160-164	22.935241964139532	27.291647452382534	26.82857426794471	22.94453631553322
165-169	22.778356502446044	27.278756105873835	26.910278125464387	23.03260926621573
170-174	22.719506979365587	27.295811612798204	27.12619371761116	22.858487690225047
175-178	22.750753942849318	27.33792745237779	26.94172998176042	22.96958862301247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	0.5
14	1.0
15	0.5
16	1.5
17	6.5
18	19.0
19	48.5
20	96.5
21	175.5
22	282.0
23	427.5
24	683.5
25	990.5
26	1321.5
27	1734.5
28	2216.0
29	2757.5
30	3396.5
31	4097.5
32	4841.5
33	5580.0
34	6291.5
35	7197.5
36	8121.0
37	8916.5
38	9753.5
39	10661.0
40	11476.5
41	12177.0
42	12826.5
43	13310.0
44	13781.5
45	14092.0
46	13989.0
47	13740.5
48	13494.0
49	13076.0
50	12371.5
51	11672.5
52	10963.0
53	10175.0
54	9450.5
55	8817.5
56	8154.0
57	7356.5
58	6657.0
59	5988.5
60	5361.5
61	4828.5
62	4226.5
63	3634.0
64	3092.0
65	2597.5
66	2167.0
67	1727.0
68	1387.0
69	1105.0
70	819.5
71	614.5
72	445.5
73	304.5
74	206.5
75	133.0
76	86.0
77	56.0
78	39.5
79	26.5
80	9.5
81	6.0
82	4.5
83	2.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	43.0
92-93	66.0
94-95	110.0
96-97	160.0
98-99	218.0
100-101	237.0
102-103	328.0
104-105	308.0
106-107	376.0
108-109	400.0
110-111	457.0
112-113	454.0
114-115	490.0
116-117	504.0
118-119	545.0
120-121	600.0
122-123	574.0
124-125	634.0
126-127	658.0
128-129	684.0
130-131	739.0
132-133	818.0
134-135	3646.0
136-137	3166.0
138-139	2932.0
140-141	3071.0
142-143	3265.0
144-145	3406.0
146-147	3164.0
148-149	3367.0
150-151	3489.0
152-153	3336.0
154-155	3505.0
156-157	3462.0
158-159	3457.0
160-161	3662.0
162-163	3604.0
164-165	3732.0
166-167	3711.0
168-169	3937.0
170-171	4214.0
172-173	4487.0
174-175	4681.0
176-177	11853.0
178-179	239489.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.67122215375777
#Duplication Level	Percentage of total
1	99.35847052830913
2	0.6186586793465603
3	0.008193653058298514
4	0.0015729856641860038
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.013104153621820757
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	8.927535196807514E-4	0.0	0.0	2.9758450656025044E-4	0.0	0.0
3	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
4	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
5	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
6	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
7	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
8	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
9	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
10-14	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
15-19	8.927535196807514E-4	2.9758450656025044E-4	0.0	5.951690131205009E-4	0.0	0.0
20-24	8.927535196807514E-4	2.9758450656025044E-4	0.0	7.142028157446011E-4	0.0	0.0
25-29	8.927535196807514E-4	2.9758450656025044E-4	0.0	8.927535196807514E-4	0.0	0.0
30-34	8.927535196807514E-4	2.9758450656025044E-4	0.0	0.0010713042236169016	0.0	0.0
35-39	8.927535196807514E-4	2.9758450656025044E-4	0.0	0.0011903380262410018	0.0	0.0
40-44	8.927535196807514E-4	2.9758450656025044E-4	0.0	0.0011903380262410018	0.0	0.0
45-49	8.927535196807514E-4	2.9758450656025044E-4	0.0	0.0011903380262410018	0.0	0.0
50-54	8.927535196807514E-4	2.9758450656025044E-4	0.0	0.0011903380262410018	0.0	0.0
55-59	8.927535196807514E-4	2.9758450656025044E-4	0.0	0.0011903380262410018	1.1903380262410018E-4	0.0
60-64	8.927535196807514E-4	2.9758450656025044E-4	0.0	0.0011903380262410018	4.1661830918435065E-4	0.0
65-69	8.927535196807514E-4	2.9758450656025044E-4	0.0	0.0011903380262410018	5.951690131205009E-4	0.0
70-74	0.0010713042236169018	2.9758450656025044E-4	0.0	0.0011903380262410018	5.951690131205009E-4	0.0
75-79	0.0050589366115242574	2.9758450656025044E-4	2.3806760524820036E-4	0.0014879225328012521	5.951690131205009E-4	0.0
80-84	0.007737197170566512	2.9758450656025044E-4	2.9758450656025044E-4	0.0014879225328012521	5.951690131205009E-4	0.0
85-89	0.007737197170566512	2.9758450656025044E-4	2.9758450656025044E-4	0.0014879225328012521	5.951690131205009E-4	0.0
90-94	0.00827284928237496	2.9758450656025044E-4	2.9758450656025044E-4	0.0014879225328012521	5.951690131205009E-4	0.0
95-99	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0014879225328012521	5.951690131205009E-4	0.0
100-104	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017259901380494527	5.951690131205009E-4	0.0
105-109	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
110-114	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
115-119	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
120-124	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
125-129	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
130-134	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
135-139	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
140-144	0.008332366183687013	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
145-149	0.008510916887623163	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
150-154	0.008629950690247263	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
155-159	0.008629950690247263	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
160-164	0.008629950690247263	2.9758450656025044E-4	2.9758450656025044E-4	0.0017855070393615028	5.951690131205009E-4	0.0
165-167	0.008629950690247263	2.9758450656025044E-4	2.9758450656025044E-4	0.001785507039361503	5.951690131205009E-4	0.0
>>END_MODULE
