##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293622
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4344116
Total Bases	723.9 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.649204809448	39.0	37.0	39.0	35.0	39.0
2	39.28334096050842	40.0	39.0	41.0	36.0	41.0
3	39.37663911368849	40.0	39.0	41.0	36.0	41.0
4	39.42540185390998	40.0	39.0	41.0	37.0	41.0
5	39.443241847132995	40.0	39.0	41.0	37.0	41.0
6	39.4375559952819	40.0	39.0	41.0	37.0	41.0
7	39.48611938539394	40.0	39.0	41.0	37.0	41.0
8	39.47034885808758	40.0	39.0	41.0	37.0	41.0
9	39.46672510586734	40.0	39.0	41.0	37.0	41.0
10-14	39.40975015400141	40.0	39.0	41.0	36.8	41.0
15-19	39.27357068733892	40.0	39.0	41.0	36.0	41.0
20-24	39.12246919741553	40.0	38.0	41.0	36.0	41.0
25-29	38.96807258369712	40.0	38.0	41.0	35.2	41.0
30-34	38.88685688872029	40.0	38.0	41.0	35.0	41.0
35-39	38.58890872159031	40.0	37.6	40.8	34.6	41.0
40-44	38.41434482872925	40.0	37.0	40.8	34.2	41.0
45-49	37.84900872812789	39.0	35.8	40.0	33.8	41.0
50-54	37.102233043500675	37.4	35.0	40.0	33.0	41.0
55-59	36.28986495756559	36.2	34.6	39.0	33.0	40.2
60-64	35.43159777501337	35.0	33.8	37.0	32.0	39.4
65-69	34.96373338096865	35.0	34.0	36.0	32.0	38.6
70-74	34.692953549122535	35.0	34.0	35.8	32.0	37.0
75-79	34.52929364685473	35.0	34.0	35.0	32.0	37.0
80-84	34.46205971479583	35.0	34.0	35.0	32.0	36.4
85-89	34.32389171928189	34.8	33.6	35.0	31.6	37.0
90-94	37.80423256241683	39.8	36.4	40.6	31.6	40.6
95-99	38.18205371160177	40.0	37.0	41.0	32.0	41.0
100-104	38.10078079120895	40.0	36.8	41.0	32.0	41.0
105-109	37.96675830126905	40.0	36.0	41.0	32.0	41.0
110-114	37.79732756228079	40.0	36.0	41.0	32.0	41.0
115-119	37.61189634358526	39.6	35.2	40.2	31.4	41.0
120-124	37.41100872570617	39.0	35.0	40.0	31.0	41.0
125-129	37.04347468423232	38.6	34.6	39.8	31.0	40.6
130-134	36.912544913414855	38.0	34.0	40.0	31.0	41.0
135-139	36.65397676265989	37.4	34.0	40.0	31.0	41.0
140-144	36.348197937533854	36.6	34.0	39.8	31.0	41.0
145-149	35.72601439129195	35.6	34.0	38.8	31.0	40.4
150-154	35.095813074487246	35.0	34.0	36.8	31.0	39.2
155-159	34.55729274752722	35.0	34.0	36.0	30.8	37.8
160-164	34.171326616667635	35.0	33.0	35.2	30.0	36.4
165-169	33.93510254327926	35.0	33.0	35.0	30.0	36.0
170-174	33.83035839326071	35.0	33.0	35.0	30.0	35.8
175-178	33.602016415533214	34.75	32.75	35.0	29.75	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	67.0
29	1177.0
30	7597.0
31	30917.0
32	102548.0
33	223461.0
34	327187.0
35	469439.0
36	764118.0
37	1206859.0
38	1036548.0
39	173307.0
40	890.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.568580581181532	29.165128187184685	29.66826852689937	20.598022704734404
2	20.781120946125746	29.736199493752007	29.773468295966314	19.70921126415593
3	18.867037620542362	28.106339701794337	31.76917927605985	21.257443401603457
4	19.88867240193402	27.369204689745853	31.898733827549723	20.843389080770404
5	20.848729637974678	28.092827171281797	29.929104103113268	21.129339087630257
6	21.089054712166984	28.29655101291034	29.385702407578435	21.228691867344242
7	21.092898992568337	28.721125310650088	29.008801790744076	21.1771739060375
8	21.092369540776534	28.786915450692383	28.999018442417285	21.121696566113794
9	21.35060389731766	28.77001903264093	28.648613434816195	21.230763635225212
10-14	21.252420515474267	28.96788667705927	28.60074178497996	21.17895102248651
15-19	21.25974996984427	28.89279660119573	28.69270986318045	21.15474356577955
20-24	21.124380656501806	28.771800753018567	28.92466039120502	21.179158199274607
25-29	21.153919462555788	28.798126016892734	28.740747254447164	21.30720726610431
30-34	21.194908239098588	28.844989406360234	28.72826600394649	21.23183635059469
35-39	21.180585417148162	28.82123773858709	28.70432097117112	21.29385587309363
40-44	21.194125571232444	28.8577699122215	28.723192474602428	21.22491204194363
45-49	21.42857603250005	28.719840814563884	28.583863782643004	21.267719370293058
50-54	21.32959156707602	28.776478344500926	28.656757784552717	21.237172303870338
55-59	21.2935289941613	28.767822958687105	28.655215468463552	21.283432578688046
60-64	21.27410041536644	28.823525891113405	28.639921217573377	21.262452475946773
65-69	21.239750503899987	28.777072251293472	28.676250818348315	21.306926426458226
70-74	21.17392813635732	28.857429221503295	28.674179050467348	21.294463591672045
75-79	21.177717169615175	28.816739700321076	28.656104026688052	21.34943910337569
80-84	21.128514984406493	28.85009976713329	28.66334140248557	21.35804384597465
85-89	21.09647164118085	28.86943626735566	28.629180252092716	21.404911839370772
90-94	20.143977532467712	28.705634522184987	30.209573847320815	20.940814098026493
95-99	20.80765820239357	28.91773920864596	28.96937443569397	21.305228153266498
100-104	20.991096732791323	29.04669445354528	28.67029729483476	21.291911518828634
105-109	20.850557885997837	29.029567515543654	28.832087559442577	21.287787039015935
110-114	20.898834479695658	28.866402944517734	28.87500716432518	21.359755411461432
115-119	20.92612357820993	28.914373022261724	28.745052939263292	21.41445046026505
120-124	20.92337394882198	28.963887099778628	28.747372873561815	21.365366077837578
125-129	20.970937147493572	28.926126527734198	28.72139334147657	21.38154298329566
130-134	21.072264347131274	28.910349047637645	28.669025493164575	21.34836111206651
135-139	21.205017228688693	28.867549190795316	28.567408857229747	21.360024723286244
140-144	21.199025824207766	28.90874067923329	28.621898979018525	21.27033451754042
145-149	21.172831894553852	28.919269442789936	28.62760144578897	21.280297216867243
150-154	21.100425609507255	28.991026166084666	28.651939410340532	21.256608814067548
155-159	21.109180470437384	28.95713065606546	28.661877930859873	21.27181094263728
160-164	21.053203429013596	29.034779355573104	28.703990177238182	21.208027038175114
165-169	20.965730885517324	29.078246513230145	28.717338997447257	21.238683603805274
170-174	20.992578895901858	29.11853498088719	28.734785314250153	21.154100808960795
175-178	20.940852893152048	29.16560582959476	28.708666707193558	21.184874570059634
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	2.0
14	4.5
15	6.0
16	20.0
17	56.5
18	141.0
19	355.0
20	761.5
21	1585.5
22	3123.5
23	5543.5
24	9134.5
25	14074.0
26	20416.0
27	28158.5
28	37674.0
29	48464.5
30	59970.0
31	72515.5
32	86261.5
33	101134.0
34	117349.0
35	134048.5
36	151080.0
37	169081.0
38	186545.0
39	202194.5
40	215575.5
41	226363.0
42	233172.5
43	235112.0
44	232019.5
45	224177.0
46	211939.0
47	195850.0
48	177234.0
49	157369.0
50	136975.5
51	117074.0
52	98648.5
53	82374.5
54	68110.0
55	55545.0
56	45129.5
57	36463.5
58	29498.0
59	23716.5
60	18997.5
61	15370.0
62	12511.5
63	10127.5
64	8113.5
65	6538.0
66	5207.5
67	4123.0
68	3185.0
69	2433.0
70	1832.0
71	1348.5
72	981.5
73	715.5
74	507.5
75	323.0
76	215.5
77	135.5
78	79.5
79	55.5
80	28.0
81	16.0
82	9.5
83	4.0
84	2.5
85	1.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1117.0
92-93	2596.0
94-95	3778.0
96-97	5148.0
98-99	6227.0
100-101	7351.0
102-103	8228.0
104-105	9220.0
106-107	10050.0
108-109	11005.0
110-111	11491.0
112-113	12377.0
114-115	12955.0
116-117	13704.0
118-119	14792.0
120-121	14860.0
122-123	15075.0
124-125	16023.0
126-127	16478.0
128-129	16917.0
130-131	17530.0
132-133	18703.0
134-135	77817.0
136-137	75489.0
138-139	65304.0
140-141	68148.0
142-143	76984.0
144-145	65512.0
146-147	71397.0
148-149	77553.0
150-151	68058.0
152-153	57933.0
154-155	65915.0
156-157	64888.0
158-159	60934.0
160-161	84741.0
162-163	58730.0
164-165	56340.0
166-167	62473.0
168-169	63733.0
170-171	64629.0
172-173	63834.0
174-175	68456.0
176-177	137061.0
178-179	2542562.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.57902759899149
#Duplication Level	Percentage of total
1	86.65611920843898
2	10.110253387593493
3	1.787676878409197
4	0.5711236434014145
5	0.2757981426017217
6	0.16907136947470772
7	0.1175670800808
8	0.07458738570353163
9	0.05247604262852601
>10	0.18532686166765577
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.6113750185308128E-4	2.3019643121868752E-5	0.0	0.0	2.3019643121868752E-5	0.0
2	4.6039286243737505E-4	2.3019643121868752E-5	0.0	0.0	2.3019643121868752E-5	0.0
3	4.6039286243737505E-4	2.3019643121868752E-5	0.0	0.0	2.3019643121868752E-5	0.0
4	4.6039286243737505E-4	2.3019643121868752E-5	0.0	0.0	4.6039286243737505E-5	0.0
5	4.6039286243737505E-4	2.3019643121868752E-5	0.0	4.6039286243737505E-5	4.6039286243737505E-5	0.0
6	4.6039286243737505E-4	2.3019643121868752E-5	0.0	4.6039286243737505E-5	4.6039286243737505E-5	0.0
7	4.6039286243737505E-4	2.3019643121868752E-5	0.0	4.6039286243737505E-5	4.6039286243737505E-5	0.0
8	4.6039286243737505E-4	2.3019643121868752E-5	0.0	4.6039286243737505E-5	4.6039286243737505E-5	0.0
9	4.834125055592438E-4	2.3019643121868752E-5	2.3019643121868752E-5	4.6039286243737505E-5	4.6039286243737505E-5	0.0
10-14	4.834125055592438E-4	3.6831428994990004E-5	2.3019643121868752E-5	1.0128642973622251E-4	5.5247143492485006E-5	0.0
15-19	4.834125055592438E-4	4.6039286243737505E-5	2.3019643121868752E-5	1.1509821560934378E-4	9.207857248747501E-5	0.0
20-24	4.834125055592438E-4	4.6039286243737505E-5	4.603928624373751E-5	1.1509821560934378E-4	1.1509821560934375E-4	0.0
25-29	4.834125055592438E-4	4.6039286243737505E-5	6.905892936560626E-5	1.5653357322870754E-4	1.5192964460433375E-4	0.0
30-34	5.294517918029813E-4	4.6039286243737505E-5	6.905892936560626E-5	1.6113750185308128E-4	1.9336500222369752E-4	0.0
35-39	7.274207226510526E-4	4.6039286243737505E-5	8.747464386310126E-5	2.2559250259431376E-4	2.348003598430613E-4	0.0
40-44	9.392014393722452E-4	6.44550007412325E-5	1.1509821560934378E-4	3.130671464574151E-4	3.545025040767788E-4	6.44550007412325E-5
45-49	0.0011325664415959425	6.905892936560626E-5	1.1509821560934378E-4	3.6831428994990004E-4	4.972242914323651E-4	1.1970214423371751E-4
50-54	0.001422613944931489	6.905892936560626E-5	1.1509821560934378E-4	4.23561433442385E-4	5.616792921735975E-4	1.3811785873121251E-4
55-59	0.0016712260906476713	6.905892936560626E-5	1.4732571597996002E-4	4.511850051886275E-4	6.123225070417089E-4	1.3811785873121251E-4
60-64	0.0018369675211251265	8.287071523872751E-5	1.7034535910182875E-4	5.248478631786076E-4	6.905892936560626E-4	1.3811785873121251E-4
65-69	0.0019336500222369751	1.1049428698497001E-4	1.8415714497495002E-4	6.583617932854463E-4	7.228167940266789E-4	1.3811785873121251E-4
70-74	0.0027071100311317656	1.3811785873121251E-4	1.8415714497495002E-4	7.366285798998001E-4	7.826678661435376E-4	1.5192964460433375E-4
75-79	0.018282200567388163	1.3811785873121251E-4	1.8876107359932378E-4	7.826678661435376E-4	8.102914378897801E-4	1.6113750185308128E-4
80-84	0.024990124573100715	1.3811785873121251E-4	2.0717678809681876E-4	7.826678661435376E-4	9.161817962503764E-4	1.6113750185308128E-4
85-89	0.0251512620749538	1.7955321635057626E-4	2.1638464534556629E-4	8.194992951385276E-4	0.0011233585843471948	1.6113750185308128E-4
90-94	0.025266360290563138	2.0717678809681876E-4	2.3019643121868755E-4	8.425189382603964E-4	0.0011279625129715688	1.6113750185308128E-4
95-99	0.025321607434055628	2.1638464534556629E-4	2.4861214571618255E-4	9.484092966209926E-4	0.0011279625129715688	1.6113750185308128E-4
100-104	0.02534923100580187	2.3019643121868755E-4	3.130671464574151E-4	0.001017468225986599	0.0011279625129715688	1.6113750185308128E-4
105-109	0.025482744935908706	2.3019643121868755E-4	3.2227500370616256E-4	0.0010635075122303365	0.001146378227469064	1.6113750185308128E-4
110-114	0.02555640779389869	2.3019643121868755E-4	3.4069071820365756E-4	0.0011325664415959428	0.0011693978705909324	1.6113750185308128E-4
115-119	0.02564388243776179	2.3019643121868755E-4	3.6831428994990004E-4	0.0011924175137128013	0.0012338528713321652	1.6113750185308128E-4
120-124	0.025901702440726725	2.440082170918088E-4	3.8212607582302125E-4	0.0012614764430784078	0.0012430607285809127	1.6113750185308128E-4
125-129	0.026288432445174113	2.532160743405563E-4	4.0054179032051625E-4	0.0012891000148246502	0.0012568725144540339	1.6113750185308128E-4
130-134	0.02680407245110398	2.532160743405563E-4	4.60392862437375E-4	0.0013627628728146303	0.0013535550155658828	1.6113750185308128E-4
135-139	0.02716317888380513	2.532160743405563E-4	5.294517918029814E-4	0.0014272178735558628	0.0014318218021802365	1.6113750185308128E-4
140-144	0.02750847353063316	2.532160743405563E-4	5.294517918029814E-4	0.0014686532311752264	0.0015008807315458428	1.6113750185308128E-4
145-149	0.02780312496259308	2.532160743405563E-4	5.294517918029814E-4	0.0015561278750383276	0.0015791475181601965	1.6113750185308128E-4
150-154	0.027978074250319285	2.532160743405563E-4	5.294517918029814E-4	0.0015929593040333177	0.0015883553754089438	1.6113750185308128E-4
155-159	0.028208270681537972	2.6242393158930376E-4	5.294517918029814E-4	0.0016343946616526814	0.0015975632326576915	1.6113750185308128E-4
160-164	0.02844767497000541	2.854435747111725E-4	5.294517918029814E-4	0.001662018233398924	0.001680433947896419	1.6113750185308128E-4
165-167	0.02861341640048286	2.9925536058429377E-4	5.294517918029814E-4	0.0016881071622703752	0.0017725125203838941	1.6113750185308128E-4
>>END_MODULE
