##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293623
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5311982
Total Bases	886.3 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.69175667387427	39.0	37.0	39.0	35.0	39.0
2	39.3205330891558	40.0	39.0	41.0	36.0	41.0
3	39.40817194034167	40.0	39.0	41.0	37.0	41.0
4	39.454662308720174	40.0	39.0	41.0	37.0	41.0
5	39.47758011981215	40.0	39.0	41.0	37.0	41.0
6	39.47408029620582	40.0	39.0	41.0	37.0	41.0
7	39.52177172287105	40.0	39.0	41.0	37.0	41.0
8	39.4998070400088	40.0	39.0	41.0	37.0	41.0
9	39.49186367724891	40.0	39.0	41.0	37.0	41.0
10-14	39.42335339238725	40.0	39.0	41.0	36.6	41.0
15-19	39.307730259628144	40.0	39.0	41.0	36.0	41.0
20-24	39.15815015939437	40.0	38.2	41.0	36.0	41.0
25-29	38.9852700931592	40.0	38.0	41.0	35.2	41.0
30-34	38.91791850951302	40.0	38.0	41.0	35.0	41.0
35-39	38.64126678140099	40.0	37.6	41.0	34.6	41.0
40-44	38.46189448684126	40.0	37.0	41.0	34.4	41.0
45-49	37.910870405810854	39.0	35.8	40.0	34.0	41.0
50-54	37.181674410794315	37.4	35.0	40.0	33.0	41.0
55-59	36.38451079088747	36.2	35.0	39.0	33.0	40.4
60-64	35.52352101343716	35.2	33.8	37.0	32.4	39.6
65-69	35.04993556077562	35.0	34.0	36.0	32.0	38.8
70-74	34.7743990096352	35.0	34.0	36.0	32.0	37.2
75-79	34.586618177546534	35.0	34.0	35.0	32.0	37.0
80-84	34.51348622792773	35.0	34.0	35.0	32.0	37.0
85-89	34.370106864066926	34.8	33.8	35.0	31.6	37.0
90-94	37.926646727102465	39.8	36.6	40.6	32.2	40.6
95-99	38.31463774534787	40.0	37.0	41.0	33.0	41.0
100-104	38.23895399900811	40.0	37.0	41.0	32.8	41.0
105-109	38.111394832806624	40.0	36.8	41.0	32.0	41.0
110-114	37.95197007648135	40.0	36.0	41.0	32.0	41.0
115-119	37.75563487787997	40.0	35.8	40.6	32.0	41.0
120-124	37.56943699449571	39.0	35.0	40.0	32.0	41.0
125-129	37.211107339134934	38.6	34.8	40.0	31.6	40.6
130-134	37.08706363736953	38.6	34.8	40.0	31.0	41.0
135-139	36.81777348449496	37.8	34.0	40.0	31.0	41.0
140-144	36.4789361278138	36.8	34.0	39.8	31.0	41.0
145-149	35.83721352059083	35.6	34.0	38.8	31.0	40.4
150-154	35.178004004241245	35.0	34.0	37.0	31.0	39.4
155-159	34.63828290399308	35.0	34.0	36.0	31.0	37.8
160-164	34.23760014577926	35.0	33.8	35.2	31.0	36.6
165-169	34.00263586887961	35.0	33.0	35.0	31.0	36.0
170-174	33.898282680192764	35.0	33.0	35.0	31.0	35.8
175-178	33.67364724406563	34.75	32.75	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	61.0
29	1200.0
30	7919.0
31	33830.0
32	109988.0
33	245811.0
34	379543.0
35	562350.0
36	916350.0
37	1471243.0
38	1339663.0
39	242389.0
40	1635.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.53843555945784	29.13968834984757	29.68253657486038	20.639339515834205
2	20.800334037276482	29.678074963356426	29.804882621966716	19.716708377400373
3	18.934683890118603	28.0840936584499	31.746041308121903	21.235181143309596
4	19.938602954603386	27.30528830858237	31.850465607752437	20.905643129061808
5	20.849317636995004	28.122779783515835	29.85412977679518	21.173772802693986
6	21.131434556818903	28.252392421510464	29.390818718888735	21.2253543027819
7	21.071005135183064	28.7259821287045	29.037447792556527	21.16556494355591
8	21.11825680132199	28.798610386857487	28.94202201739389	21.141110794426638
9	21.34284340571937	28.758550010146873	28.65361742566146	21.2449891584723
10-14	21.281642144118713	28.925564883314742	28.59535292100011	21.197440051566442
15-19	21.277214418271747	28.885515801823125	28.689408209591072	21.147861570314056
20-24	21.12693529458496	28.738903859237475	28.92178098495063	21.21237986122694
25-29	21.178290890292924	28.779461225583976	28.71228102806071	21.329966856062388
30-34	21.20140843850751	28.8258695153711	28.735485172954277	21.237236873167113
35-39	21.18193548095607	28.784626905738765	28.73940084887336	21.294036764431805
40-44	21.207138879612167	28.824517854164416	28.729555182980665	21.238788083242753
45-49	21.431552290651588	28.695936846171545	28.5838694483528	21.28864141482407
50-54	21.33820107071146	28.763629093622683	28.649946479487316	21.248223356178542
55-59	21.301841760758975	28.75740919302814	28.63563920209067	21.305109844122214
60-64	21.28501941459892	28.796701494846932	28.64049991133253	21.277779179221618
65-69	21.247677420593668	28.757134342699203	28.668346391234007	21.326841845473123
70-74	21.179209568104714	28.82645310168596	28.667721389116153	21.326615941093173
75-79	21.183422684790724	28.81515788268861	28.6606919978268	21.340727434693868
80-84	21.143565622022063	28.837413229186392	28.655695745956972	21.363325402834572
85-89	21.124894625019436	28.85134023421013	28.618090196841784	21.405674943928652
90-94	20.151758983840175	28.68594388775864	30.20476532821027	20.95753180019091
95-99	20.850807642047357	28.899124306127057	28.941546076340995	21.308521975484588
100-104	21.012093713466744	28.976040643416724	28.67239381707758	21.339471826038952
105-109	20.905590100565405	28.99476134511061	28.805592244200124	21.29405631012386
110-114	20.94848872382443	28.84405739204883	28.856650561768042	21.3508033223587
115-119	20.978610604201286	28.880667298705276	28.72372593206935	21.416996165024088
120-124	20.94835045949733	28.934498945792637	28.731189194199253	21.385961400510787
125-129	20.999502220193868	28.900554556033764	28.720947298442724	21.378995925329647
130-134	21.085143813759384	28.896434286970013	28.665150569184057	21.35327133008655
135-139	21.206496346542245	28.876171878220013	28.56135799071286	21.355973784524878
140-144	21.221721998771052	28.878097266936383	28.626497787085743	21.27368294720683
145-149	21.184697935881427	28.87964196909459	28.620357133368902	21.315302961655085
150-154	21.117818172914752	28.956962950337083	28.66064945912935	21.264569417618816
155-159	21.14743698969641	28.938933344050227	28.649280685517226	21.26434898073614
160-164	21.07994743185965	29.004478682199224	28.711284733020264	21.20428915292086
165-169	21.005557943677314	29.036094794294907	28.715837325621003	21.24250993640678
170-174	21.03177837160431	29.094915480125493	28.692426722746973	21.180879425523223
175-178	20.972523067739505	29.137095030136624	28.700973491065803	21.18940841105807
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	3.0
15	9.0
16	26.5
17	68.0
18	188.5
19	448.0
20	951.5
21	1947.5
22	3721.5
23	6544.5
24	10930.0
25	16818.0
26	24506.0
27	34157.0
28	45263.0
29	58064.5
30	72504.0
31	88385.0
32	105241.5
33	123304.0
34	143157.0
35	163895.0
36	185088.0
37	206735.5
38	228094.0
39	247559.5
40	263671.0
41	276368.0
42	285074.0
43	288130.5
44	284063.5
45	273868.0
46	258812.5
47	239384.5
48	216933.5
49	192633.0
50	167697.0
51	143592.5
52	121406.5
53	101281.0
54	83382.5
55	68353.5
56	55826.5
57	45213.5
58	36409.5
59	29131.0
60	23358.0
61	18814.0
62	15266.0
63	12291.0
64	9934.5
65	8090.0
66	6475.5
67	5026.0
68	3881.0
69	3097.5
70	2317.0
71	1698.0
72	1231.5
73	865.0
74	605.0
75	432.0
76	303.0
77	183.5
78	103.0
79	64.0
80	40.0
81	21.5
82	12.0
83	5.0
84	3.5
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1352.0
92-93	3172.0
94-95	4591.0
96-97	6050.0
98-99	7727.0
100-101	8857.0
102-103	10072.0
104-105	11150.0
106-107	12223.0
108-109	13502.0
110-111	14469.0
112-113	15030.0
114-115	15853.0
116-117	16376.0
118-119	17439.0
120-121	18211.0
122-123	19727.0
124-125	19238.0
126-127	19662.0
128-129	20722.0
130-131	21497.0
132-133	22788.0
134-135	92872.0
136-137	87157.0
138-139	80473.0
140-141	82279.0
142-143	89131.0
144-145	89035.0
146-147	72589.0
148-149	81685.0
150-151	89298.0
152-153	87613.0
154-155	79041.0
156-157	72911.0
158-159	70363.0
160-161	69046.0
162-163	81316.0
164-165	72771.0
166-167	79319.0
168-169	76517.0
170-171	86306.0
172-173	87447.0
174-175	88862.0
176-177	164709.0
178-179	3131534.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.4120528794895
#Duplication Level	Percentage of total
1	84.71524897115779
2	11.257184971351467
3	2.1715658509172218
4	0.708131247021164
5	0.3656951005061364
6	0.20809619518129563
7	0.13721699468533913
8	0.10973423166604301
9	0.06344306165228762
>10	0.2620119129497239
>50	0.0016714629115410157
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.412682497794609E-5	1.8825364995589217E-5	1.8825364995589217E-5	0.0	0.0	0.0
2	3.9533266490737355E-4	1.8825364995589217E-5	1.8825364995589217E-5	1.8825364995589217E-5	0.0	0.0
3	3.9533266490737355E-4	5.647609498676765E-5	3.7650729991178434E-5	1.8825364995589217E-5	0.0	0.0
4	3.9533266490737355E-4	5.647609498676765E-5	3.7650729991178434E-5	1.8825364995589217E-5	0.0	0.0
5	3.9533266490737355E-4	5.647609498676765E-5	3.7650729991178434E-5	7.530145998235687E-5	0.0	0.0
6	3.9533266490737355E-4	5.647609498676765E-5	3.7650729991178434E-5	1.129521899735353E-4	0.0	0.0
7	3.9533266490737355E-4	5.647609498676765E-5	3.7650729991178434E-5	1.129521899735353E-4	1.8825364995589217E-5	0.0
8	3.9533266490737355E-4	5.647609498676765E-5	3.7650729991178434E-5	1.3177755496912452E-4	1.8825364995589217E-5	0.0
9	3.9533266490737355E-4	5.647609498676765E-5	7.530145998235687E-5	1.5060291996471373E-4	1.8825364995589217E-5	0.0
10-14	3.953326649073736E-4	5.6476094986767644E-5	7.530145998235687E-5	1.6942828496030294E-4	1.8825364995589217E-5	0.0
15-19	4.179231029020806E-4	7.530145998235687E-5	7.530145998235687E-5	2.1084408795059924E-4	1.8825364995589217E-5	0.0
20-24	4.3298339489855197E-4	9.036175197882824E-5	7.530145998235687E-5	2.4096467194354196E-4	3.7650729991178434E-5	0.0
25-29	4.405135408967877E-4	9.412682497794609E-5	8.283160598059256E-5	3.0120583992942747E-4	4.518087598941412E-5	0.0
30-34	4.8192934388708393E-4	9.412682497794609E-5	9.412682497794609E-5	3.5015178891795943E-4	1.2048233597177098E-4	0.0
35-39	7.379543078270973E-4	9.412682497794609E-5	9.412682497794609E-5	4.5557383289325905E-4	1.995488689532457E-4	0.0
40-44	0.0011709377027256493	9.412682497794609E-5	9.412682497794609E-5	5.384054388738516E-4	3.3509149692148804E-4	0.0
45-49	0.0014759086156541947	9.412682497794609E-5	9.412682497794609E-5	6.061767528579727E-4	3.878025189091379E-4	3.7650729991178434E-5
50-54	0.0017281685065950903	9.412682497794609E-5	1.0165697097618179E-4	6.25002117853562E-4	4.442786138959055E-4	6.400624098500333E-5
55-59	0.001923952302549218	1.0165697097618179E-4	1.1295218997353529E-4	6.626528478447405E-4	5.158150008791445E-4	9.036175197882824E-5
60-64	0.0020820853685121674	1.1295218997353529E-4	1.6189813896206728E-4	7.003035778359189E-4	6.024116798588549E-4	1.2048233597177098E-4
65-69	0.002176212193490114	1.1295218997353529E-4	1.769584309585386E-4	7.492495268244508E-4	6.212370448544441E-4	1.3177755496912452E-4
70-74	0.003245492925239581	1.1295218997353529E-4	2.070790149514814E-4	8.546715707997504E-4	6.513576288473869E-4	1.3177755496912452E-4
75-79	0.02052717799119048	1.2048233597177098E-4	2.183742339488349E-4	9.111476657865181E-4	7.153638698323903E-4	1.3177755496912452E-4
80-84	0.028027203405433226	1.6566321196118512E-4	2.560249639400133E-4	9.525634687768143E-4	8.847921547926931E-4	1.3177755496912452E-4
85-89	0.028094974719417347	2.070790149514814E-4	2.8991062093207387E-4	9.751539067715216E-4	0.0010956362427432924	1.3177755496912452E-4
90-94	0.028230517347385593	2.2590437994707057E-4	3.539168619170772E-4	0.0010278649287591712	0.001125756826736235	1.3177755496912452E-4
95-99	0.028339704464360006	2.2590437994707057E-4	4.141580299029628E-4	0.0010542204397529962	0.001129521899735353	1.3177755496912452E-4
100-104	0.0285279581143159	2.2590437994707057E-4	4.141580299029628E-4	0.0010956362427432926	0.001129521899735353	1.3177755496912452E-4
105-109	0.028591964355300904	2.2590437994707057E-4	4.2545324890031627E-4	0.0011144616077388815	0.001129521899735353	1.3177755496912452E-4
110-114	0.028678561034280615	2.447297449426598E-4	4.367484678976698E-4	0.0011332869727344707	0.001159642483728296	1.3177755496912452E-4
115-119	0.028780218005256798	2.447297449426598E-4	4.6310397889149475E-4	0.0011483472647309423	0.001193528140720356	1.3177755496912452E-4
120-124	0.02903247789619769	2.447297449426598E-4	4.706341248897305E-4	0.0011935281407203563	0.001223648724713299	1.3177755496912452E-4
125-129	0.02949558187508918	2.447297449426598E-4	4.969896358835553E-4	0.001250004235707124	0.0012537693087062418	1.3177755496912452E-4
130-134	0.02985326381000538	2.447297449426598E-4	5.271102198764981E-4	0.0012688296007027134	0.0013328358416877166	1.3177755496912452E-4
135-139	0.030282482131904813	2.447297449426598E-4	5.459355848720873E-4	0.0012801248197000668	0.0013930770096736021	1.3177755496912452E-4
140-144	0.030674049723813067	2.447297449426598E-4	5.647609498676765E-4	0.0013177755496912452	0.001464613396656841	1.3177755496912452E-4
145-149	0.030892423957761907	2.6355510993824905E-4	5.647609498676765E-4	0.0013855468636753663	0.0014759086156541945	1.3177755496912452E-4
150-154	0.031035496731728385	2.710852559364847E-4	5.647609498676765E-4	0.0014457880316612521	0.0015060291996471375	1.3177755496912452E-4
155-159	0.031246340819678982	2.8238047493383823E-4	5.647609498676765E-4	0.0014683784696559589	0.0015286196376418444	1.3177755496912452E-4
160-164	0.03155884187860576	2.8238047493383823E-4	5.798212418641479E-4	0.0014759086156541945	0.0015850957326286122	1.468378469655959E-4
165-167	0.03190899366752372	2.8238047493383823E-4	5.961365581936585E-4	0.0015060291996471375	0.0016127062679554764	1.5060291996471373E-4
>>END_MODULE
