##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293624
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5814536
Total Bases	975.1 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.7365985867144	39.0	37.0	39.0	35.0	39.0
2	39.38479235488438	40.0	39.0	41.0	36.0	41.0
3	39.4584692570482	40.0	39.0	41.0	37.0	41.0
4	39.49408000913572	40.0	39.0	41.0	37.0	41.0
5	39.51136926489061	40.0	39.0	41.0	37.0	41.0
6	39.49759499296246	40.0	39.0	41.0	37.0	41.0
7	39.550132461128456	40.0	39.0	41.0	37.0	41.0
8	39.51228799684102	40.0	39.0	41.0	37.0	41.0
9	39.51227578606444	40.0	39.0	41.0	37.0	41.0
10-14	39.46561187341518	40.0	39.0	41.0	37.0	41.0
15-19	39.34502474488076	40.0	39.0	41.0	36.0	41.0
20-24	39.18993704742734	40.0	38.2	41.0	36.0	41.0
25-29	39.02238486441566	40.0	38.0	41.0	35.4	41.0
30-34	38.96787943182397	40.0	38.0	41.0	35.0	41.0
35-39	38.695061996348464	40.0	37.8	41.0	34.8	41.0
40-44	38.52827599657135	40.0	37.2	41.0	34.4	41.0
45-49	37.98626356428097	39.0	36.2	40.2	34.0	41.0
50-54	37.26534591926166	37.8	35.0	40.0	33.2	41.0
55-59	36.470697300696045	36.4	35.0	39.0	33.0	40.6
60-64	35.61508054984955	35.4	34.2	37.6	32.6	39.6
65-69	35.125198364925424	35.0	34.0	36.4	32.2	39.0
70-74	34.831836590228356	35.0	34.0	36.0	32.0	37.2
75-79	34.63650231076048	35.0	34.0	35.0	32.0	37.0
80-84	34.567156106695364	35.0	34.0	35.0	32.0	37.0
85-89	34.42126446547068	34.8	33.8	35.0	31.6	37.2
90-94	38.250690825610256	39.8	37.8	40.6	32.8	40.6
95-99	38.574726153238444	40.0	38.0	41.0	33.0	41.0
100-104	38.484727903246586	40.0	38.0	41.0	33.0	41.0
105-109	38.3728971862874	40.0	37.4	41.0	33.0	41.0
110-114	38.200933218317424	40.0	37.0	41.0	33.0	41.0
115-119	38.01451120700914	40.0	36.2	41.0	32.6	41.0
120-124	37.812470639666515	39.8	36.0	40.6	32.0	41.0
125-129	37.43884584631659	38.8	35.4	40.4	32.0	40.8
130-134	37.332973546048976	39.0	35.0	40.0	32.0	41.0
135-139	37.04468225597502	38.0	35.0	40.0	32.0	41.0
140-144	36.70510232461511	37.0	35.0	40.0	32.0	41.0
145-149	36.03343726974655	35.8	34.0	39.0	32.0	40.6
150-154	35.356311152424574	35.0	34.0	37.2	32.0	39.4
155-159	34.788794409556765	35.0	34.0	36.0	31.2	38.0
160-164	34.3731188836336	35.0	34.0	35.2	31.0	36.6
165-169	34.131568238134335	35.0	34.0	35.0	31.0	36.0
170-174	34.02463680702867	35.0	34.0	35.0	31.0	35.8
175-178	33.78734558939575	34.75	33.5	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	69.0
29	1064.0
30	6987.0
31	29734.0
32	99099.0
33	228750.0
34	371481.0
35	579485.0
36	981749.0
37	1642392.0
38	1573871.0
39	297958.0
40	1897.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.556360817097012	29.15594640741755	29.64138497035705	20.646307805128387
2	20.79581242596142	29.665393764867908	29.794157263795423	19.744636545375247
3	18.933703394389507	28.073211688774478	31.71582392816899	21.277260988667024
4	19.912182158645162	27.317313023773522	31.813630528730062	20.956874288851253
5	20.8748901030108	28.044679747446743	29.895816278375438	21.18461387116702
6	21.11146959963787	28.265729200059987	29.366040557664448	21.256760642637694
7	21.0899545552732	28.694946595910665	29.03244558121233	21.18265326760381
8	21.127704773003384	28.75534694427896	28.922737773057044	21.194210509660614
9	21.399385952722625	28.730512632478327	28.623178186531135	21.246923228267914
10-14	21.289592153182987	28.920770290183086	28.589197831090907	21.20043972554302
15-19	21.289296342820823	28.834641319616907	28.699978811722897	21.176083525839378
20-24	21.139396161619775	28.728411003044783	28.90472773751852	21.227465097816918
25-29	21.183255207294273	28.75968778936101	28.716630183388663	21.340426819956054
30-34	21.232707132606972	28.801517438364815	28.70594661379687	21.259828815231344
35-39	21.208622665677883	28.744997021258445	28.714346252220295	21.332034060843377
40-44	21.214621424650222	28.804489300607994	28.720131752559446	21.260757522182338
45-49	21.464388559981398	28.67878365530801	28.566017993525193	21.290809791185403
50-54	21.352355544793255	28.738530469155233	28.64312130838987	21.26599267766164
55-59	21.332794224681038	28.731596123921154	28.631622540474428	21.303987110923384
60-64	21.311729775170367	28.756929185751023	28.625372686659777	21.305968352418834
65-69	21.266322884577548	28.738265615691432	28.65974172315727	21.335669776573745
70-74	21.19661826842245	28.80320974880885	28.65436210215226	21.345809880616443
75-79	21.21208639863955	28.77588856617278	28.640063454762338	21.37196158042533
80-84	21.157007197134906	28.814536533955593	28.641029997922445	21.387426270987056
85-89	21.122225401992523	28.82524762079038	28.623800764153838	21.42872621306326
90-94	20.16898144046261	28.6622987923974	30.210021658155544	20.958698108984443
95-99	20.898766713024774	28.866090091379633	28.919787857968306	21.31535533762729
100-104	21.060333823852226	28.994572348192417	28.626553441967097	21.31854038598826
105-109	20.97065058609136	28.98510141094394	28.758361683621214	21.285886319343483
110-114	20.98799531186552	28.824559752904115	28.806722177667144	21.38072275756322
115-119	21.010858082048195	28.85609312270987	28.70346677777694	21.429582017465
120-124	20.97595013125549	28.899273279988584	28.726348672596846	21.39842791615908
125-129	21.027678222097677	28.87682640321218	28.69078538221287	21.404709992477272
130-134	21.1189836942056	28.872886553795237	28.65687672865723	21.351253023341936
135-139	21.2246480148701	28.834205223503357	28.562312128013744	21.378834633612794
140-144	21.230802215356242	28.869896324201978	28.60079200035997	21.298509460081814
145-149	21.21060626423072	28.85507957573392	28.59876911536285	21.33554504467251
150-154	21.112745501770345	28.959630307183488	28.65313639604973	21.27448779499644
155-159	21.165579103696583	28.919351041418494	28.626422196743352	21.288647658141574
160-164	21.106220258951208	29.002461834231603	28.674792064895566	21.216525841921623
165-169	21.040122682409503	29.032557324867874	28.68561699567084	21.241702997051775
170-174	21.02280784340707	29.08397041180189	28.700941711715156	21.19228003307589
175-178	20.969497249637744	29.10875849742263	28.717804836313082	21.203939416626547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	1.0
11	0.5
12	1.0
13	0.5
14	5.5
15	11.0
16	23.5
17	66.0
18	173.5
19	431.0
20	1005.0
21	2095.0
22	3998.5
23	7089.0
24	11695.0
25	18232.5
26	26800.0
27	36941.0
28	49210.5
29	63615.5
30	78924.5
31	95709.0
32	114044.5
33	133934.0
34	155465.0
35	178067.5
36	201627.5
37	225455.0
38	248699.0
39	269930.5
40	288509.5
41	302856.0
42	312113.0
43	315419.5
44	310936.0
45	300379.0
46	284343.5
47	263023.5
48	238294.0
49	211434.0
50	184232.0
51	158523.0
52	134337.5
53	111499.0
54	91925.5
55	75532.0
56	61057.0
57	49282.0
58	39871.0
59	32238.0
60	26090.0
61	20928.0
62	16879.5
63	13693.0
64	11043.5
65	8901.0
66	7143.0
67	5541.0
68	4338.0
69	3402.5
70	2515.5
71	1871.5
72	1352.5
73	960.0
74	674.0
75	446.5
76	286.0
77	173.5
78	107.5
79	65.5
80	44.5
81	26.5
82	11.5
83	7.5
84	4.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1494.0
92-93	3259.0
94-95	5170.0
96-97	6197.0
98-99	8161.0
100-101	9021.0
102-103	10252.0
104-105	11372.0
106-107	12817.0
108-109	13775.0
110-111	14667.0
112-113	15069.0
114-115	16320.0
116-117	16910.0
118-119	17553.0
120-121	18782.0
122-123	18997.0
124-125	20181.0
126-127	20704.0
128-129	21250.0
130-131	21838.0
132-133	23668.0
134-135	95789.0
136-137	86277.0
138-139	80551.0
140-141	84797.0
142-143	82212.0
144-145	85432.0
146-147	80592.0
148-149	80515.0
150-151	85958.0
152-153	83060.0
154-155	76172.0
156-157	76508.0
158-159	71532.0
160-161	76077.0
162-163	78872.0
164-165	82442.0
166-167	81811.0
168-169	81958.0
170-171	84958.0
172-173	80542.0
174-175	94006.0
176-177	186612.0
178-179	3590406.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.71860373187225
#Duplication Level	Percentage of total
1	83.56768949448731
2	11.89058323545336
3	2.4764889201055214
4	0.7872498570745
5	0.3944376376536468
6	0.22807458105505732
7	0.15440342922209171
8	0.09639996766845413
9	0.08333262135412219
>10	0.31509904293782187
>50	0.006241212988071104
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.599138435121909E-5	0.0	0.0	0.0	0.0	0.0
2	6.191379673287774E-4	0.0	0.0	1.7198276870243817E-5	0.0	0.0
3	6.363362441990212E-4	1.7198276870243817E-5	0.0	3.4396553740487634E-5	0.0	0.0
4	6.363362441990212E-4	1.7198276870243817E-5	0.0	5.159483061073145E-5	0.0	0.0
5	6.363362441990212E-4	1.7198276870243817E-5	0.0	5.159483061073145E-5	0.0	0.0
6	6.363362441990212E-4	3.4396553740487634E-5	1.7198276870243817E-5	6.879310748097527E-5	0.0	0.0
7	6.363362441990212E-4	3.4396553740487634E-5	3.4396553740487634E-5	6.879310748097527E-5	0.0	0.0
8	6.363362441990212E-4	3.4396553740487634E-5	5.159483061073145E-5	6.879310748097527E-5	0.0	0.0
9	6.363362441990212E-4	3.4396553740487634E-5	5.159483061073145E-5	6.879310748097527E-5	0.0	0.0
10-14	6.397758995730699E-4	3.4396553740487634E-5	6.879310748097527E-5	8.599138435121909E-5	1.0318966122146289E-5	0.0
15-19	6.53534521069265E-4	3.4396553740487634E-5	9.631035047336537E-5	1.0662931659551167E-4	3.4396553740487634E-5	0.0
20-24	6.707327979395088E-4	3.4396553740487634E-5	1.0318966122146288E-4	1.4446552571004806E-4	4.8155175236682684E-5	0.0
25-29	6.707327979395088E-4	4.4715519862633926E-5	1.1006897196956042E-4	1.5478449183219434E-4	1.1694828271765795E-4	0.0
30-34	7.739224591609717E-4	5.159483061073144E-5	1.4790518108409682E-4	1.685431133283894E-4	1.7198276870243818E-4	0.0
35-39	0.0010009397138481901	5.159483061073144E-5	1.6510345795434065E-4	2.0637932244292576E-4	2.1669828856507207E-4	0.0
40-44	0.0011866811040468234	5.8474141358828976E-5	1.960603563207795E-4	2.0637932244292576E-4	2.786120852979498E-4	6.8793107480975265E-6
45-49	0.0013173880082606763	6.879310748097527E-5	2.235775993131696E-4	2.2013794393912085E-4	3.2676726053463255E-4	3.4396553740487634E-5
50-54	0.0014721725000928704	6.879310748097527E-5	2.4077587618341343E-4	2.3045691006126715E-4	4.196379556339491E-4	7.911207360312156E-5
55-59	0.001599439748932675	6.879310748097527E-5	2.4765518693151096E-4	3.0612932829033994E-4	4.8155175236682686E-4	1.0318966122146288E-4
60-64	0.0017232673423984303	7.223276285502403E-5	2.717327745498523E-4	3.3708622665677885E-4	4.884310631149244E-4	1.1694828271765795E-4
65-69	0.001874612178856576	8.599138435121909E-5	2.923707067941449E-4	3.6116381427512016E-4	6.053793458325824E-4	1.2038793809170672E-4
70-74	0.0027620432653611567	8.599138435121909E-5	2.923707067941449E-4	3.8868105726751017E-4	6.707327979395088E-4	1.30706904213853E-4
75-79	0.019870889095879706	1.0318966122146288E-4	3.095689836643887E-4	4.2307761100799794E-4	7.292069392983377E-4	1.4102587033599931E-4
80-84	0.02720767400872572	1.1350862734360919E-4	3.4396553740487635E-4	4.7467244161872936E-4	9.183879848710198E-4	1.5478449183219434E-4
85-89	0.027303984359199084	1.2038793809170672E-4	3.5084484815297386E-4	5.193879614813633E-4	0.0012141983470392134	1.5478449183219434E-4
90-94	0.02742093264191674	1.2038793809170672E-4	3.6116381427512016E-4	5.503448598478021E-4	0.0012382759346575547	1.5478449183219434E-4
95-99	0.027448449884909132	1.2038793809170672E-4	3.646034696491689E-4	5.503448598478021E-4	0.0012382759346575547	1.5478449183219434E-4
100-104	0.027520682647764156	1.2038793809170672E-4	4.093189895118028E-4	5.744224474661435E-4	0.0012451552454056524	1.5478449183219434E-4
105-109	0.02762387230898562	1.2038793809170672E-4	4.299569217560954E-4	6.53534521069265E-4	0.00127955179914614	1.5478449183219434E-4
110-114	0.02772018265945898	1.2038793809170672E-4	4.471551986263392E-4	6.948103855578503E-4	0.001303629386764481	1.5478449183219434E-4
115-119	0.02778209645619186	1.2038793809170672E-4	4.677931308706318E-4	7.670431484128742E-4	0.0013483449066271152	1.5478449183219434E-4
120-124	0.02800911371087908	1.2038793809170672E-4	5.021896846111194E-4	7.911207360312156E-4	0.0014274569802302367	1.5478449183219434E-4
125-129	0.02839779476814659	1.2038793809170672E-4	5.331465829775583E-4	7.911207360312156E-4	0.001468732844718822	1.5478449183219434E-4
130-134	0.028917182729627955	1.2038793809170672E-4	5.606638259699483E-4	8.255172897717033E-4	0.001510008709207407	1.5478449183219434E-4
135-139	0.029391855171246685	1.2038793809170672E-4	5.847414135882898E-4	8.805517757564834E-4	0.0015478449183219435	1.5478449183219434E-4
140-144	0.02974957933014775	1.3414655958790179E-4	5.916207243363873E-4	9.115086741229223E-4	0.0015788018166883824	1.5478449183219434E-4
145-149	0.029938760375720443	1.3758621496195054E-4	6.088190012066311E-4	9.803017816038978E-4	0.0016613535456655524	1.5478449183219434E-4
150-154	0.030165777630407654	1.3758621496195054E-4	6.53534521069265E-4	0.0010078190245962875	0.0017370259638946257	1.5478449183219434E-4
155-159	0.03039279488509487	1.513448364581456E-4	6.604138318173625E-4	0.0010662931659551167	0.0018264570036198933	1.5478449183219434E-4
160-164	0.030750519043995943	1.5478449183219434E-4	6.707327979395088E-4	0.0010662931659551167	0.0018986897664749172	1.6166380258029187E-4
165-167	0.031083019063487326	1.5478449183219434E-4	6.707327979395087E-4	0.0010777586838686124	0.0019548708042510473	1.7198276870243818E-4
>>END_MODULE
