##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293625
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5226284
Total Bases	879.2 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.788045578847225	39.0	37.0	39.0	35.0	39.0
2	39.441022340156024	40.0	39.0	41.0	37.0	41.0
3	39.50960950457342	40.0	39.0	41.0	37.0	41.0
4	39.53810087626314	40.0	39.0	41.0	37.0	41.0
5	39.55974168261809	40.0	39.0	41.0	37.0	41.0
6	39.54650914492974	40.0	39.0	41.0	37.0	41.0
7	39.58401380407188	40.0	39.0	41.0	37.0	41.0
8	39.57088803440456	40.0	39.0	41.0	37.0	41.0
9	39.566129203847325	40.0	39.0	41.0	37.0	41.0
10-14	39.510269744238926	40.0	39.0	41.0	37.0	41.0
15-19	39.381836540073216	40.0	39.0	41.0	36.2	41.0
20-24	39.23223716889476	40.0	38.6	41.0	36.0	41.0
25-29	39.058371301674384	40.0	38.0	41.0	35.4	41.0
30-34	39.001582080116584	40.0	38.0	41.0	35.2	41.0
35-39	38.723724275221166	40.0	37.8	41.0	34.8	41.0
40-44	38.55775262117405	40.0	37.2	41.0	34.6	41.0
45-49	38.019565182450854	39.2	36.2	40.4	34.0	41.0
50-54	37.30542603501838	37.8	35.0	40.0	33.4	41.0
55-59	36.510606580124616	36.4	35.0	39.2	33.0	40.6
60-64	35.6494081454433	35.4	34.2	37.6	32.6	39.6
65-69	35.16499126339097	35.0	34.0	36.4	32.6	39.0
70-74	34.86817715990941	35.0	34.0	36.0	32.4	37.4
75-79	34.66830788376598	35.0	34.0	35.0	32.0	37.0
80-84	34.590848067192674	35.0	34.0	35.0	32.0	37.0
85-89	34.45149547938841	34.8	33.8	35.0	31.6	37.2
90-94	38.391397991302505	39.8	37.8	40.6	32.8	40.6
95-99	38.6977872420744	40.0	38.0	41.0	33.0	41.0
100-104	38.618529716394214	40.0	38.0	41.0	33.0	41.0
105-109	38.48514938204187	40.0	38.0	41.0	33.0	41.0
110-114	38.32066628587403	40.0	37.2	41.0	33.0	41.0
115-119	38.131906888877424	40.0	36.8	41.0	33.0	41.0
120-124	37.93038396528699	40.0	36.0	41.0	32.8	41.0
125-129	37.55754653251364	39.2	35.6	40.6	32.2	40.8
130-134	37.43673445855889	39.0	35.0	40.0	32.0	41.0
135-139	37.15821974730613	38.2	35.0	40.0	32.0	41.0
140-144	36.7804849229157	37.2	35.0	40.0	32.0	41.0
145-149	36.09268735409877	36.0	34.4	39.0	32.0	40.6
150-154	35.39106063738817	35.0	34.0	37.2	32.0	39.4
155-159	34.821969813784456	35.0	34.0	36.0	32.0	38.0
160-164	34.408108936125494	35.0	34.0	35.2	31.0	36.6
165-169	34.1601412224196	35.0	34.0	35.0	31.0	36.0
170-174	34.04147477115556	35.0	34.0	35.0	31.0	35.8
175-178	33.79533201184806	34.75	33.75	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	54.0
29	934.0
30	6295.0
31	26113.0
32	87135.0
33	194218.0
34	309972.0
35	495971.0
36	860031.0
37	1479199.0
38	1477137.0
39	287434.0
40	1791.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.619124410384128	29.05125324226544	29.623667600153382	20.705954747197055
2	20.880725195951847	29.637482387103343	29.735200000612288	19.746592416332522
3	18.942617737574157	28.046160522466824	31.65534058233345	21.35588115762557
4	19.972508191288497	27.29541678178989	31.779424922181803	20.95265010473981
5	20.929784910272765	27.99224075844328	29.876390184689544	21.201584146594406
6	21.162493274380036	28.181916635223036	29.31285402783316	21.342736062563763
7	21.097609697444685	28.67178668438225	28.978122888078794	21.25248073009427
8	21.192801615832586	28.676914610840132	28.913641126276335	21.216642647050946
9	21.450039837100316	28.657206535274394	28.600569735590337	21.292183892034952
10-14	21.33662847254378	28.861585019107267	28.538169758857347	21.26361674949161
15-19	21.327459433892226	28.805078330990046	28.637111951818923	21.2303502832988
20-24	21.183104477292087	28.683557954370638	28.852622628238343	21.280714940098928
25-29	21.237881446932466	28.71757447547818	28.650823414877568	21.393720662711786
30-34	21.262089851986612	28.762971931873583	28.658017819161756	21.316920396978045
35-39	21.245431744620078	28.71869573103949	28.664397112747796	21.371475411592634
40-44	21.278445641300777	28.74639418753363	28.666719986897	21.308440184268594
45-49	21.498119887859136	28.63647670122787	28.509005633830842	21.356397777082147
50-54	21.399663699867823	28.70209885264559	28.58301232768828	21.315225119798313
55-59	21.372975521422106	28.702703488750323	28.562994280448596	21.36132670937898
60-64	21.35511962227847	28.732024513019194	28.569603182681995	21.343252682020342
65-69	21.316197129738836	28.696006569868764	28.592560220607986	21.395236079784414
70-74	21.253793326195055	28.7420469304768	28.607435034146633	21.396724709181512
75-79	21.262223790364242	28.730822894431302	28.590623854348518	21.416329460855934
80-84	21.223741381065402	28.742712795554166	28.58986231900142	21.443683504379017
85-89	21.192124270322854	28.79166535917298	28.53620660492235	21.480003765581817
90-94	20.23101494454676	28.620760712575787	30.1822874786885	20.96593686418895
95-99	20.978513226834895	28.80482885099874	28.87440774242825	21.34225017973812
100-104	21.134052490736067	28.94001319827146	28.55793205071827	21.368002260274206
105-109	21.0118580802812	28.9373607799377	28.730707283332706	21.320073856448392
110-114	21.04976223191856	28.75943785301444	28.777530043571314	21.41326987149569
115-119	21.084069237910214	28.7947550263548	28.64620692786126	21.474968807873722
120-124	21.04392451177822	28.85985017140658	28.681560352180362	21.414664964634834
125-129	21.089468591949643	28.841494576915856	28.646752064116754	21.422284767017743
130-134	21.15167797763178	28.829624791785402	28.613512172942425	21.405185057640395
135-139	21.29435694597785	28.784491817068886	28.500436396838452	21.42071484011481
140-144	21.290563623123447	28.83043204488634	28.55822927768718	21.320775054303034
145-149	21.270524009909206	28.80789109377234	28.55249028910097	21.36909460721748
150-154	21.169665961907498	28.894491042062135	28.60005126642116	21.33579172960921
155-159	21.200564541816775	28.877572024451258	28.59711011204149	21.324753321690473
160-164	21.159040453113388	28.933563107903264	28.624469101982932	21.28292733700042
165-169	21.082218417401002	28.973141696417965	28.638218773399764	21.30642111278127
170-174	21.095700134951862	29.010920460470015	28.648170382452598	21.245209022125525
175-178	21.0210106956869	29.07751030577957	28.640863316289078	21.260615682244453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	6.0
15	12.5
16	26.0
17	60.5
18	154.5
19	360.0
20	850.0
21	1889.5
22	3563.5
23	6227.5
24	10394.5
25	16141.0
26	23455.5
27	32534.5
28	43329.5
29	55644.0
30	69437.5
31	84151.5
32	100620.0
33	118892.0
34	137912.5
35	157908.0
36	179142.0
37	200277.5
38	221086.0
39	240759.0
40	258110.5
41	271607.5
42	279867.5
43	282887.0
44	279842.0
45	271422.5
46	257077.5
47	237858.0
48	215901.0
49	191952.5
50	167370.5
51	144304.5
52	122121.0
53	101521.5
54	84057.0
55	69001.0
56	56651.5
57	46037.0
58	36951.0
59	29762.0
60	24158.5
61	19524.0
62	15627.0
63	12730.0
64	10428.0
65	8353.5
66	6595.0
67	5284.5
68	4160.0
69	3141.5
70	2363.5
71	1776.0
72	1280.0
73	902.0
74	625.5
75	405.5
76	269.5
77	173.0
78	109.0
79	62.5
80	36.5
81	18.5
82	8.5
83	6.0
84	4.0
85	2.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1383.0
92-93	2884.0
94-95	4149.0
96-97	5234.0
98-99	6621.0
100-101	7840.0
102-103	8720.0
104-105	9648.0
106-107	10638.0
108-109	11653.0
110-111	12658.0
112-113	13262.0
114-115	14030.0
116-117	14480.0
118-119	15436.0
120-121	15736.0
122-123	16428.0
124-125	16860.0
126-127	17322.0
128-129	18014.0
130-131	18823.0
132-133	20228.0
134-135	85124.0
136-137	73427.0
138-139	66365.0
140-141	68850.0
142-143	75646.0
144-145	67027.0
146-147	65819.0
148-149	67110.0
150-151	67856.0
152-153	73686.0
154-155	76369.0
156-157	61272.0
158-159	68561.0
160-161	67656.0
162-163	65524.0
164-165	65763.0
166-167	65300.0
168-169	66294.0
170-171	79874.0
172-173	67553.0
174-175	77063.0
176-177	156155.0
178-179	3335943.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.60131364202816
#Duplication Level	Percentage of total
1	84.99848503908323
2	11.160596155669216
3	2.102135792271413
4	0.6776778701932069
5	0.32550264146037516
6	0.18733107531800194
7	0.12995118985261195
8	0.08532374730574965
9	0.07213498676980902
>10	0.25763575693706564
>50	0.003225745139284585
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.530724315785365E-4	0.0	0.0	0.0	0.0	0.0
2	5.548875644721948E-4	0.0	1.9134053947317062E-5	0.0	0.0	0.0
3	5.548875644721948E-4	0.0	1.9134053947317062E-5	1.9134053947317062E-5	0.0	0.0
4	5.548875644721948E-4	0.0	1.9134053947317062E-5	1.9134053947317062E-5	0.0	0.0
5	5.548875644721948E-4	0.0	1.9134053947317062E-5	1.9134053947317062E-5	0.0	0.0
6	5.548875644721948E-4	0.0	1.9134053947317062E-5	1.9134053947317062E-5	0.0	0.0
7	5.548875644721948E-4	1.9134053947317062E-5	1.9134053947317062E-5	1.9134053947317062E-5	0.0	0.0
8	5.548875644721948E-4	1.9134053947317062E-5	1.9134053947317062E-5	3.8268107894634125E-5	0.0	0.0
9	5.548875644721948E-4	1.9134053947317062E-5	1.9134053947317062E-5	7.653621578926825E-5	0.0	0.0
10-14	5.548875644721948E-4	1.9134053947317062E-5	1.9134053947317062E-5	1.3393837763121943E-4	1.530724315785365E-5	0.0
15-19	5.66367996840585E-4	1.9134053947317062E-5	3.06144863157073E-5	2.257818365783413E-4	1.9134053947317062E-5	0.0
20-24	5.893288615773656E-4	1.9134053947317062E-5	5.3575351052487774E-5	2.793571876308291E-4	2.6787675526243887E-5	0.0
25-29	6.199433478930727E-4	2.6787675526243887E-5	7.653621578926825E-5	3.06144863157073E-4	3.8268107894634125E-5	0.0
30-34	6.773455097350238E-4	3.8268107894634125E-5	7.653621578926825E-5	3.367593494727803E-4	3.8268107894634125E-5	7.653621578926824E-6
35-39	8.304179413135604E-4	3.8268107894634125E-5	1.0332389131551213E-4	3.9033470052526805E-4	4.592172947356095E-5	1.9134053947317062E-5
40-44	9.375686434185361E-4	3.8268107894634125E-5	1.1480432368390237E-4	4.247759976304387E-4	1.1863113447336579E-4	1.9134053947317062E-5
45-49	0.001086814264207609	3.8268107894634125E-5	1.4159199921014626E-4	4.974854026302436E-4	2.563963228940486E-4	3.444129710517071E-5
50-54	0.0012513671281545358	3.8268107894634125E-5	1.530724315785365E-4	5.548875644721948E-4	3.71200646577951E-4	3.8268107894634125E-5
55-59	0.001465668532364487	3.8268107894634125E-5	1.568992423679999E-4	5.893288615773656E-4	4.324296192093656E-4	6.888259421034142E-5
60-64	0.0015422047481537552	3.8268107894634125E-5	1.913405394731706E-4	6.352505910509265E-4	5.01312213419707E-4	7.653621578926825E-5
65-69	0.0016837967473639017	4.209491868409754E-5	2.0664778263102427E-4	6.735186989455605E-4	5.625411860511216E-4	8.418983736819508E-5
70-74	0.002636672633940291	5.740216184195119E-5	2.525695121045852E-4	7.156136176296581E-4	6.352505910509265E-4	9.56702697365853E-5
75-79	0.01948994735073716	5.740216184195119E-5	3.214521063149266E-4	7.959766442083898E-4	6.888259421034142E-4	9.56702697365853E-5
80-84	0.027047898659927398	8.801664815765848E-5	3.444129710517071E-4	8.189375089451703E-4	7.88323022629463E-4	1.1097751289443893E-4
85-89	0.02719331746992701	1.1097751289443893E-4	3.444129710517071E-4	8.457251844714141E-4	0.0010447193455235117	1.1480432368390237E-4
90-94	0.02728516092887413	1.3393837763121943E-4	3.6354702499902415E-4	9.069541571028288E-4	0.0010523729671024383	1.1480432368390237E-4
95-99	0.0273616971446634	1.4159199921014626E-4	3.826810789463412E-4	9.528758865763896E-4	0.001075333831839219	1.1480432368390237E-4
100-104	0.02748798190071569	1.6837967473639013E-4	3.979883221041949E-4	9.83490372892097E-4	0.0010906410749970724	1.1480432368390237E-4
105-109	0.027641054332294225	1.913405394731706E-4	4.0564194368312176E-4	0.0010102780484183408	0.0010906410749970724	1.1480432368390237E-4
110-114	0.02776733908834652	1.9516735026263402E-4	4.209491868409754E-4	0.0010561997778919016	0.0011212555613127797	1.1480432368390237E-4
115-119	0.02787831660124096	2.104745934204877E-4	4.209491868409754E-4	0.001075333831839219	0.0011977917771020482	1.1480432368390237E-4
120-124	0.02810792524860876	2.104745934204877E-4	4.515636731566826E-4	0.0011021215073654628	0.001243713506575609	1.1480432368390237E-4
125-129	0.02841407011176584	2.104745934204877E-4	4.6304410552507286E-4	0.0011212555613127797	0.001262847560522926	1.1480432368390237E-4
130-134	0.02885415335255413	2.2960864736780475E-4	4.974854026302436E-4	0.0011595236692074139	0.0013508642086805845	1.1480432368390237E-4
135-139	0.029432001781763105	2.2960864736780475E-4	5.166194565775607E-4	0.0011863113447336576	0.0014159199921014627	1.1480432368390237E-4
140-144	0.029749627077288565	2.2960864736780475E-4	5.319266997354142E-4	0.001224579452628292	0.001484802586311804	1.1480432368390237E-4
145-149	0.029994542967814224	2.2960864736780475E-4	5.587143752616582E-4	0.0012934620468386332	0.0015345511265748286	1.1480432368390237E-4
150-154	0.030208844372024174	2.4108907973619496E-4	5.740216184195119E-4	0.0013623446410489747	0.0015842996668378527	1.1480432368390237E-4
155-159	0.030629793558865154	2.487427013151218E-4	5.778484292089753E-4	0.0014197468028909258	0.0016876235581533647	1.1480432368390237E-4
160-164	0.03093211161123276	2.487427013151218E-4	6.161165371036094E-4	0.0014388808568382432	0.0017565061523637064	1.1480432368390237E-4
165-167	0.031067325592460473	2.551207192975608E-4	6.314237802614631E-4	0.0014669441359609747	0.0018496252149073158	1.1480432368390236E-4
>>END_MODULE
