##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293636
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7717865
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.27316764416066	39.0	39.0	39.0	37.0	39.0
2	39.98110954260019	41.0	40.0	41.0	38.0	41.0
3	40.04982167995942	41.0	40.0	41.0	38.0	41.0
4	40.08329816600835	41.0	40.0	41.0	38.0	41.0
5	40.091504451036656	41.0	40.0	41.0	38.0	41.0
6	40.08052848812463	41.0	40.0	41.0	38.0	41.0
7	40.09841542965574	41.0	40.0	41.0	38.0	41.0
8	40.08156608077493	41.0	40.0	41.0	38.0	41.0
9	40.05616514411693	41.0	40.0	41.0	38.0	41.0
10-14	39.9858927306969	41.0	40.0	41.0	38.0	41.0
15-19	39.825517186423966	41.0	40.0	41.0	38.0	41.0
20-24	39.59509198463565	40.0	39.2	41.0	37.0	41.0
25-29	39.27809483581275	40.0	38.4	41.0	35.6	41.0
30-34	39.04558395359338	40.0	38.0	41.0	35.0	41.0
35-39	38.61840446807504	40.0	37.0	41.0	35.0	41.0
40-44	38.00152739131871	39.0	35.4	41.0	34.2	41.0
45-49	37.46226089728182	37.6	35.0	40.4	34.0	41.0
50-54	36.7690622212231	36.4	35.0	39.2	34.0	41.0
55-59	36.12395547214159	35.0	35.0	38.0	33.8	40.4
60-64	35.32419559036081	34.8	34.6	36.4	32.4	38.8
65-69	35.182904857755354	35.0	34.8	36.0	33.0	38.2
70-74	35.159723420920166	35.0	35.0	35.2	33.0	37.0
75-79	35.044813662845876	35.0	35.0	35.0	33.0	37.0
80-84	34.99147279720493	35.0	35.0	35.0	33.0	36.0
85-89	34.85099529468319	35.0	34.6	35.0	32.6	37.0
90-94	39.478036031106036	40.6	39.8	40.6	37.0	40.6
95-99	39.761452494124	41.0	40.0	41.0	37.0	41.0
100-104	39.6232158704798	41.0	39.6	41.0	36.4	41.0
105-109	39.43720491255402	40.4	39.0	41.0	35.6	41.0
110-114	39.200889188524414	40.0	38.4	41.0	35.0	41.0
115-119	38.85543398872963	40.0	38.0	41.0	35.0	41.0
120-124	38.50748982508215	40.0	37.0	41.0	35.0	41.0
125-129	37.773939672999546	38.8	35.6	40.4	34.0	40.6
130-134	37.60331317012811	38.6	35.0	40.4	34.0	41.0
135-139	37.021116264203165	36.8	35.0	39.8	33.8	41.0
140-144	36.311380139618464	35.6	35.0	38.8	33.0	40.8
145-149	35.77322853067864	35.0	35.0	37.0	33.2	39.4
150-154	35.38938625095828	35.0	35.0	36.0	33.6	38.2
155-159	34.99804370322547	35.0	35.0	35.2	33.0	36.8
160-164	34.74498757590821	35.0	35.0	35.0	33.0	36.0
165-169	34.5939754065853	35.0	35.0	35.0	33.0	36.0
170-174	34.504570887364785	35.0	35.0	35.0	33.0	35.0
175-178	34.24074858794654	35.0	34.25	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	199.0
31	2343.0
32	15014.0
33	64573.0
34	210305.0
35	606411.0
36	1650592.0
37	2787413.0
38	2040981.0
39	338300.0
40	1728.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.545980656567586	26.000675057156354	26.18836945191449	24.264974834361574
2	24.71252088498568	25.982392799044813	25.97663991272198	23.32844640324753
3	22.291916222945076	26.04098413226974	27.563387024779523	24.103712620005663
4	23.334147461765657	25.25217790153106	27.534894689140067	23.87877994756322
5	23.963077871924423	25.790189385276886	26.135971541352433	24.110761201446255
6	23.712516350052766	26.14102475231168	26.301444764841055	23.8450141327945
7	23.83166847308161	26.042331655192207	26.07639547983801	24.04960439188817
8	23.91658833109934	26.238681293336953	25.826676159792893	24.01805421577081
9	23.933173228606616	26.437764848180162	26.019086884779664	23.609975038433557
10-14	23.88428665181368	26.1703826122898	25.990874419285642	23.954456316610877
15-19	23.8376442189647	26.204096599253806	26.088201853751002	23.870057328030487
20-24	23.79962852420974	26.13592748771843	26.107261528933197	23.957182459138636
25-29	23.811836563609237	26.075286364817213	26.018908078853414	24.09396899272014
30-34	23.786632702178647	26.16688682686209	26.06155717934947	23.984923291609793
35-39	23.76153767913795	26.157184661820338	26.042717772337298	24.03855988670442
40-44	23.85083439526346	26.12385938339165	25.95025178595376	24.075054435391134
45-49	24.009196843945833	26.056848623291547	25.948388576374427	23.985565956388196
50-54	23.990854983858878	26.10457425725897	25.92271308192097	23.98185767696118
55-59	23.967617987617043	26.03002514296376	25.920448206855134	24.081908662564064
60-64	23.912185559089206	26.11515231220033	25.97560594801801	23.997056180692457
65-69	23.914235348765494	26.097826277085694	25.95108103082912	24.03685734331969
70-74	23.878881011782404	26.086304956098612	25.95875932009694	24.076054712022042
75-79	23.865359137533503	26.092288476152408	26.019115390072255	24.023236996241838
80-84	23.857613472119557	26.138290835613216	25.955820683569875	24.048275008697352
85-89	23.842759623289602	26.06676069094238	25.95623271461732	24.1342469711507
90-94	23.54920067137659	25.864469667935342	26.636981949762767	23.949347710925306
95-99	23.766917297329794	26.234923559303958	26.094989914726174	23.903169228640074
100-104	23.850789116867425	26.218267124382567	26.04009999387415	23.890843764875864
105-109	23.769356738811855	26.179973757641307	26.09046754535883	23.96020195818801
110-114	23.760739065112173	26.116732013766676	26.156289223279494	23.96623969784166
115-119	23.785545038492078	26.17644226561714	26.01978563515746	24.01822706073332
120-124	23.741486975805216	26.16531761735208	26.05306161450564	24.040133792337066
125-129	23.728130078331358	26.195338766358763	26.092513298323443	23.98401785698644
130-134	23.830423788903232	26.194084391105598	25.99415359381321	23.98133822617796
135-139	23.89986619416655	26.15367822613347	25.950443114060484	23.996012465639502
140-144	23.836899448997954	26.27495699590783	26.03390857692923	23.854234978164985
145-149	23.76532531926669	26.329255628287935	26.065612641415804	23.839806411029578
150-154	23.676871485602977	26.386760441215472	26.166091896741957	23.77027617643959
155-159	23.619980385860462	26.445402145652963	26.24014488674756	23.694472581739014
160-164	23.571433867818865	26.555166753834904	26.24231993729512	23.63107944105111
165-169	23.496638425136723	26.532291543922824	26.321812185815002	23.649257845125447
170-174	23.414430926122197	26.6885624956679	26.41066022536997	23.486346352839934
175-178	23.35612821918353	26.74439871909289	26.394293605135704	23.505179456587875
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	2.5
12	6.0
13	19.5
14	40.5
15	68.5
16	130.0
17	287.5
18	583.0
19	1136.5
20	2218.0
21	3865.0
22	6389.0
23	10094.5
24	15031.0
25	21127.5
26	28604.0
27	37220.5
28	46566.5
29	56965.0
30	68005.0
31	79660.5
32	91811.5
33	104955.5
34	118339.0
35	132740.0
36	147711.5
37	162723.5
38	179783.5
39	197107.0
40	214863.0
41	232915.0
42	250091.5
43	265872.0
44	279508.0
45	290588.5
46	298646.0
47	302908.0
48	303276.5
49	299455.5
50	292419.0
51	282734.0
52	269893.0
53	256365.0
54	242068.5
55	226861.0
56	212873.5
57	199697.0
58	186394.0
59	173239.0
60	160402.5
61	147693.5
62	134502.0
63	121326.5
64	107897.0
65	94013.0
66	80281.0
67	66996.5
68	54592.0
69	43338.5
70	33484.0
71	25231.0
72	18468.0
73	12859.0
74	8758.5
75	5862.0
76	3739.0
77	2284.0
78	1333.5
79	777.5
80	440.0
81	239.5
82	120.0
83	67.0
84	35.5
85	14.5
86	8.5
87	4.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	972.0
92-93	2285.0
94-95	3691.0
96-97	4806.0
98-99	6081.0
100-101	8050.0
102-103	8462.0
104-105	9934.0
106-107	10039.0
108-109	11238.0
110-111	11760.0
112-113	13143.0
114-115	12845.0
116-117	13488.0
118-119	13939.0
120-121	14417.0
122-123	14585.0
124-125	15361.0
126-127	15976.0
128-129	16774.0
130-131	17450.0
132-133	18996.0
134-135	90037.0
136-137	96903.0
138-139	92344.0
140-141	100853.0
142-143	97805.0
144-145	100577.0
146-147	87312.0
148-149	82297.0
150-151	80585.0
152-153	81422.0
154-155	74264.0
156-157	72930.0
158-159	80214.0
160-161	78214.0
162-163	79232.0
164-165	81068.0
166-167	86144.0
168-169	86009.0
170-171	89753.0
172-173	94610.0
174-175	99575.0
176-177	231497.0
178-179	5409928.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.04822635295673
#Duplication Level	Percentage of total
1	94.3270753653209
2	5.070762520676022
3	0.4759495671466434
4	0.0744046554550652
5	0.02462047866024313
6	0.006194957490364917
7	0.007274125340404202
8	0.007320997703774804
9	0.0021051621271667297
>10	0.004292170079409456
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.2956951177560116E-5	0.0	0.0	0.0	0.0	0.0
2	7.774170706536069E-5	0.0	0.0	0.0	0.0	0.0
3	9.069865824292081E-5	0.0	1.2956951177560116E-5	2.591390235512023E-5	0.0	0.0
4	9.069865824292081E-5	1.2956951177560116E-5	2.591390235512023E-5	3.8870853532680344E-5	0.0	0.0
5	9.069865824292081E-5	1.2956951177560116E-5	3.8870853532680344E-5	6.478475588780058E-5	0.0	0.0
6	1.0365560942048093E-4	1.2956951177560116E-5	6.478475588780058E-5	6.478475588780058E-5	0.0	0.0
7	1.0365560942048093E-4	1.2956951177560116E-5	6.478475588780058E-5	1.0365560942048093E-4	0.0	0.0
8	1.2956951177560115E-4	1.2956951177560116E-5	6.478475588780058E-5	1.4252646295316126E-4	0.0	0.0
9	1.2956951177560115E-4	1.2956951177560116E-5	6.478475588780058E-5	1.4252646295316126E-4	0.0	0.0
10-14	1.2956951177560115E-4	1.2956951177560116E-5	8.292448753638474E-5	1.6066619460174543E-4	5.182780471024046E-6	0.0
15-19	1.373436824821372E-4	2.0731121884096185E-5	1.243867313045771E-4	1.788059262503296E-4	1.2956951177560116E-5	0.0
20-24	1.554834141307214E-4	3.36880730616563E-5	1.5289202389520936E-4	1.9694565789891375E-4	1.2956951177560116E-5	0.0
25-29	1.684403653082815E-4	5.182780471024046E-5	1.684403653082815E-4	2.124939993119859E-4	1.2956951177560116E-5	0.0
30-34	2.358165114315941E-4	5.441919494575248E-5	1.9435426766340169E-4	2.669131942577384E-4	1.2956951177560116E-5	0.0
35-39	3.912999255623155E-4	9.329004847843283E-5	2.1767677978300993E-4	2.9800987708388264E-4	2.5913902355120235E-5	1.0365560942048092E-5
40-44	5.597402908705969E-4	1.321609020111132E-4	2.4359068213813018E-4	3.1096682826144275E-4	8.551587777189678E-5	2.3322512119608206E-5
45-49	7.204064854723425E-4	1.6584897507276948E-4	3.031926575549067E-4	3.342893403810509E-4	1.2697812154008914E-4	3.8870853532680344E-5
50-54	8.292448753638474E-4	1.8139731648584163E-4	3.472462915586111E-4	3.757515841492433E-4	1.5289202389520934E-4	5.441919494575248E-5
55-59	8.836640703095998E-4	2.0990260907647386E-4	3.576118525006592E-4	4.146224376819237E-4	1.788059262503296E-4	6.478475588780058E-5
60-64	9.536316066684244E-4	2.4359068213813018E-4	3.9907409626885153E-4	4.794071935697242E-4	2.1508538954749791E-4	7.515031682984869E-5
65-69	9.873196797300808E-4	2.772787551997865E-4	4.275793888594838E-4	5.54557510399573E-4	2.4359068213813018E-4	9.069865824292081E-5
70-74	0.0018217473355649522	2.8505292590632253E-4	4.405363400370439E-4	6.037939248743013E-4	2.617304137867143E-4	9.588143871394484E-5
75-79	0.017528163553003323	2.8764431614183454E-4	4.5867607168562807E-4	6.271164369939096E-4	3.5502046226514717E-4	1.2956951177560115E-4
80-84	0.02455342248147642	3.109668282614427E-4	4.949555349827964E-4	6.582131198200539E-4	4.975469252183085E-4	1.5548341413072138E-4
85-89	0.024654486700661386	3.5502046226514717E-4	5.079124861603566E-4	6.996753635882462E-4	6.400733881714697E-4	1.5548341413072138E-4
90-94	0.024690766163958554	3.7575158414924337E-4	5.260522178089407E-4	6.996753635882462E-4	6.478475588780057E-4	1.5548341413072138E-4
95-99	0.024701131724900606	3.7575158414924337E-4	5.441919494575248E-4	7.178150952368304E-4	6.478475588780057E-4	1.5548341413072138E-4
100-104	0.024763325090552894	3.8352575485577946E-4	5.441919494575248E-4	7.540945585339987E-4	6.582131198200539E-4	1.5548341413072138E-4
105-109	0.02478405621243699	3.9907409626885153E-4	5.441919494575248E-4	8.033309730087271E-4	6.76352851468638E-4	1.5548341413072138E-4
110-114	0.024791830383143525	4.3794494980153186E-4	5.59740290870597E-4	8.629329484255037E-4	6.867184124106861E-4	1.5548341413072138E-4
115-119	0.024835884017147227	4.6126746192114013E-4	5.701058518126451E-4	8.99212411722672E-4	6.919011928817101E-4	1.5548341413072138E-4
120-124	0.024905851553506057	4.897727545117724E-4	5.804714127546932E-4	9.329004847843283E-4	7.100409245302942E-4	1.6066619460174543E-4
125-129	0.02514166806493765	4.923641447472844E-4	6.141594858163494E-4	9.484488261974004E-4	7.281806561788785E-4	1.710317555437935E-4
130-134	0.02543967794202153	5.027297056893324E-4	6.582131198200539E-4	9.691799480814967E-4	7.489117780629747E-4	1.9176287742788973E-4
135-139	0.0256055269170943	5.364177787509888E-4	6.841270221751741E-4	9.899110699655928E-4	7.77417070653607E-4	1.9953704813442576E-4
140-144	0.025843934818761405	5.441919494575248E-4	7.048581440592702E-4	9.976852406721288E-4	8.214707046573114E-4	2.099026090764739E-4
145-149	0.02602792352548276	5.467833396930368E-4	7.126323147658063E-4	0.0010002766309076408	8.422018265414075E-4	2.2026817001852194E-4
150-154	0.026245600305265772	5.778800225191812E-4	7.152237050013184E-4	0.0010572872160889055	8.499759972479436E-4	2.3322512119608208E-4
155-159	0.02639849232916098	5.960197541677653E-4	7.385462171209266E-4	0.0010780183379730016	8.603415581899917E-4	2.3322512119608208E-4
160-164	0.02660580354800194	6.012025346387893E-4	7.437289975919506E-4	0.0010987494598570977	8.836640703095998E-4	2.3322512119608208E-4
165-167	0.02696773438422845	6.089767053453254E-4	7.515031682984867E-4	0.0011358927198994366	9.156245498809149E-4	2.3322512119608205E-4
>>END_MODULE
