##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293651
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8690380
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.36705805730014	39.0	39.0	39.0	37.0	39.0
2	40.215261588100866	41.0	40.0	41.0	38.0	41.0
3	40.26275571378927	41.0	40.0	41.0	39.0	41.0
4	40.272961711685795	41.0	40.0	41.0	39.0	41.0
5	40.27937949778951	41.0	40.0	41.0	39.0	41.0
6	40.280771381688716	41.0	40.0	41.0	39.0	41.0
7	40.30505098741367	41.0	40.0	41.0	39.0	41.0
8	40.26735033450781	41.0	40.0	41.0	39.0	41.0
9	40.27964622950895	41.0	40.0	41.0	39.0	41.0
10-14	40.22428641785514	41.0	40.0	41.0	38.4	41.0
15-19	40.12810056637339	41.0	40.0	41.0	38.0	41.0
20-24	39.979239987204245	41.0	40.0	41.0	38.0	41.0
25-29	39.753511791199	41.0	39.8	41.0	37.0	41.0
30-34	39.40455632550015	40.4	38.8	41.0	35.4	41.0
35-39	38.91262510960395	40.0	37.6	41.0	35.0	41.0
40-44	38.37122593028154	39.6	36.0	41.0	35.0	41.0
45-49	37.8400548192369	38.8	35.0	41.0	35.0	41.0
50-54	37.32884324966227	36.8	35.0	40.4	35.0	41.0
55-59	36.69926030852506	35.6	35.0	39.0	34.4	41.0
60-64	35.97345190889236	35.0	34.8	37.4	33.6	40.2
65-69	35.6134264784739	35.0	35.0	36.4	33.8	39.0
70-74	35.39394314172683	35.0	35.0	36.0	34.0	37.6
75-79	35.192614454143545	35.0	35.0	35.0	34.0	37.0
80-84	35.101473606447584	35.0	35.0	35.0	34.0	36.0
85-89	34.98903822387514	35.0	34.8	35.0	33.8	36.0
90-94	39.67828969203859	40.6	39.8	40.6	37.8	40.6
95-99	39.98645032429592	41.0	40.0	41.0	38.0	41.0
100-104	39.87546188999863	41.0	40.0	41.0	37.4	41.0
105-109	39.74743837745368	41.0	40.0	41.0	36.8	41.0
110-114	39.55518821595915	41.0	39.2	41.0	35.8	41.0
115-119	39.28550008035508	40.2	38.6	41.0	35.0	41.0
120-124	38.903654410024615	40.0	37.8	41.0	35.0	41.0
125-129	37.98195031153517	39.4	36.0	40.4	34.2	40.6
130-134	37.92888147865186	39.0	35.0	40.8	34.4	41.0
135-139	37.64375636527767	38.0	35.0	41.0	34.8	41.0
140-144	37.06805512756447	36.4	35.0	40.0	34.2	41.0
145-149	36.434294072398586	35.2	35.0	38.6	34.0	41.0
150-154	35.85830468431251	35.0	35.0	36.8	34.0	39.4
155-159	35.37410932663618	35.0	35.0	36.0	34.0	38.0
160-164	35.024587980886686	35.0	35.0	35.2	34.0	36.6
165-169	34.806308419353435	35.0	35.0	35.0	33.4	36.0
170-174	34.685978433779916	35.0	35.0	35.0	33.0	36.0
175-178	34.51340063469061	35.0	34.75	35.0	32.75	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	34.0
30	431.0
31	2988.0
32	15482.0
33	56832.0
34	162781.0
35	438395.0
36	1370528.0
37	2900663.0
38	2754299.0
39	975659.0
40	12287.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.999040318144893	26.150594105205986	27.207026620239848	23.64333895640927
2	24.054989540158196	26.566835972650217	26.76666612967442	22.611508357517163
3	22.31636591265284	25.95306534351777	27.796966300668096	23.933602443161288
4	22.87226795606176	25.24230240794994	28.12902312672173	23.75640650926657
5	23.74936423953843	25.744167688869762	26.551462651805792	23.955005419786016
6	23.686018332915246	26.091908524138184	26.487760028905527	23.734313114041043
7	23.61421479843229	26.196587490995793	26.31518989963615	23.874007810935773
8	23.80909695548411	26.227080979197687	26.097592970618084	23.866229094700117
9	23.89446721547274	26.38862742480766	26.11227587286172	23.60462948685788
10-14	23.790685792796175	26.30073023274011	26.101309724085713	23.807274250378004
15-19	23.808832295020473	26.27426878916687	26.159392339575483	23.757506576237173
20-24	23.797854639267786	26.153753921002306	26.187517691976648	23.860873747753264
25-29	23.81730373125226	26.128095664401325	26.073267221916645	23.981333382429767
30-34	23.80047822995082	26.184060996181984	26.122883003965303	23.89257776990189
35-39	23.77392012777347	26.191722341255502	26.09030215019366	23.944055380777364
40-44	23.81785376473756	26.151022164738478	26.049763071350156	23.9813609991738
45-49	23.96319838718215	26.096918661784642	25.991802429813195	23.948080521220017
50-54	23.947438431921274	26.12655603092155	25.993632039105307	23.932373498051867
55-59	23.895396979188483	26.082203540006304	26.014581640848846	24.007817839956367
60-64	23.894655929890295	26.154598533090613	26.037298714210426	23.913446822808666
65-69	23.908464301906246	26.114492116570275	26.01599930037582	23.961044281147657
70-74	23.854600144067348	26.128963290442996	26.012975266904327	24.003461298585332
75-79	23.84291365855118	26.142435658739892	26.049074954144697	23.96557572856423
80-84	23.856250244523256	26.166158441863303	25.993026772131945	23.9845645414815
85-89	23.78918758443244	26.152722895891777	25.997717015826694	24.060372503849084
90-94	23.143600086312308	26.014531539955403	27.225624397612023	23.616243976120263
95-99	23.602610629808858	26.336486268441465	26.25716631564739	23.803736786102288
100-104	23.677462619524974	26.371287828072017	26.172971657668597	23.778277894734416
105-109	23.623024014407395	26.31420472912537	26.22352769100626	23.839243565460976
110-114	23.658105131537436	26.230101507709318	26.22728812038519	23.884505240368057
115-119	23.711738403690003	26.24490114257581	26.093364713179184	23.949995740555
120-124	23.644677388790882	26.235128932815215	26.124719624499892	23.995474053894007
125-129	23.640607156794367	26.260763061325743	26.14878436132454	23.949845420555356
130-134	23.81882587370502	26.21380006835822	26.031798053219617	23.935576004717138
135-139	23.85933242073773	26.148340128184643	26.027645077547607	23.964682373530025
140-144	23.846387855619344	26.23760684032946	26.064689772671446	23.85131553137975
145-149	23.83005176397838	26.25409408010377	26.06042629084732	23.85542786507053
150-154	23.750577790144458	26.300878699108303	26.110596876488547	23.837946634258692
155-159	23.70723408972495	26.34781074356073	26.180853270521897	23.764101896192425
160-164	23.688128458749787	26.42723427409168	26.15804826649566	23.72658900066287
165-169	23.573689713453962	26.4227868581302	26.240011953331354	23.763511475084485
170-174	23.548392456990662	26.52010705704309	26.28226596346742	23.64923452249883
175-178	23.492776827082917	26.569067812500723	26.24838207025756	23.689773290158794
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.5
12	4.0
13	6.5
14	9.5
15	24.0
16	55.0
17	100.0
18	207.5
19	424.0
20	797.5
21	1476.0
22	2721.0
23	4916.5
24	8158.5
25	12996.5
26	19948.0
27	29371.5
28	41586.0
29	56535.5
30	74396.5
31	94158.5
32	115355.5
33	137315.0
34	159879.0
35	182386.0
36	203314.0
37	223192.5
38	242196.5
39	259433.0
40	273357.0
41	285973.0
42	297317.0
43	305824.5
44	312429.5
45	316747.0
46	319018.5
47	318123.0
48	314292.5
49	308494.0
50	299026.5
51	287942.5
52	275956.5
53	262673.5
54	249926.0
55	237538.5
56	225754.5
57	214199.0
58	202712.0
59	192227.0
60	181466.0
61	169483.0
62	156969.0
63	143193.5
64	128119.5
65	112534.0
66	96257.0
67	80371.5
68	65790.5
69	51973.0
70	39934.0
71	30019.0
72	21609.5
73	15192.5
74	10450.0
75	6823.0
76	4293.5
77	2594.0
78	1530.0
79	883.5
80	471.0
81	251.0
82	133.0
83	71.0
84	34.5
85	14.0
86	6.5
87	2.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	760.0
92-93	1738.0
94-95	3234.0
96-97	3468.0
98-99	4712.0
100-101	4869.0
102-103	6004.0
104-105	6763.0
106-107	6481.0
108-109	6900.0
110-111	8085.0
112-113	8434.0
114-115	8061.0
116-117	9239.0
118-119	8571.0
120-121	9595.0
122-123	10237.0
124-125	10602.0
126-127	11246.0
128-129	11331.0
130-131	12359.0
132-133	13532.0
134-135	92748.0
136-137	94092.0
138-139	93676.0
140-141	80143.0
142-143	84163.0
144-145	84374.0
146-147	77909.0
148-149	74385.0
150-151	69514.0
152-153	75201.0
154-155	67678.0
156-157	74784.0
158-159	71609.0
160-161	75120.0
162-163	70242.0
164-165	77480.0
166-167	86502.0
168-169	82513.0
170-171	85994.0
172-173	89892.0
174-175	98719.0
176-177	154028.0
178-179	6663393.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.138586594192
#Duplication Level	Percentage of total
1	94.47222222169576
2	5.028072767972294
3	0.39336759660143367
4	0.06610917828509355
5	0.013297999629988
6	0.0010288040535303803
7	0.003104121879712584
8	0.003121898291114598
9	0.0010465804604590139
>10	0.015869878261634395
>50	0.002758952868946172
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.1863255691926016E-4	1.150697667996106E-5	0.0	0.0	1.150697667996106E-5	0.0
2	6.674046474377415E-4	1.150697667996106E-5	1.150697667996106E-5	0.0	1.150697667996106E-5	0.0
3	7.134325541575858E-4	2.301395335992212E-5	1.150697667996106E-5	0.0	1.150697667996106E-5	0.0
4	7.134325541575858E-4	2.301395335992212E-5	1.150697667996106E-5	2.301395335992212E-5	1.150697667996106E-5	0.0
5	7.249395308375468E-4	2.301395335992212E-5	2.301395335992212E-5	2.301395335992212E-5	1.150697667996106E-5	0.0
6	7.249395308375468E-4	2.301395335992212E-5	3.452093003988318E-5	2.301395335992212E-5	1.150697667996106E-5	0.0
7	7.249395308375468E-4	3.452093003988318E-5	3.452093003988318E-5	2.301395335992212E-5	1.150697667996106E-5	0.0
8	7.364465075175078E-4	3.452093003988318E-5	4.602790671984424E-5	3.452093003988318E-5	1.150697667996106E-5	0.0
9	7.364465075175078E-4	4.602790671984424E-5	4.602790671984424E-5	4.602790671984424E-5	1.150697667996106E-5	0.0
10-14	7.364465075175078E-4	6.904186007976636E-5	5.7534883399805294E-5	6.213767407178972E-5	1.6109767351945482E-5	0.0
15-19	7.433506935254845E-4	8.054883675972743E-5	1.1506976679961059E-4	1.0356279011964954E-4	2.301395335992212E-5	0.0
20-24	7.525562748694533E-4	8.975441810369627E-5	1.2427534814357942E-4	1.6109767351945485E-4	2.9918139367898756E-5	0.0
25-29	7.778716235653677E-4	1.127683714636184E-4	1.4268651083151716E-4	2.0252278956731464E-4	4.602790671984424E-5	0.0
30-34	8.100911582692588E-4	1.4959069683949378E-4	1.7720744087140034E-4	2.3704371960719785E-4	5.293209272782088E-5	4.602790671984424E-6
35-39	8.814344136850174E-4	1.7950883620739254E-4	2.2093395225525236E-4	2.830716263270421E-4	6.904186007976636E-5	2.761674403190654E-5
40-44	9.182567390608927E-4	2.2093395225525236E-4	2.623590683031122E-4	2.9918139367898756E-4	1.1506976679961059E-4	3.9123720711867605E-5
45-49	9.7809301779669E-4	2.531534869591433E-4	2.738660449830732E-4	3.2219534703890965E-4	1.311795341515561E-4	9.205581343968848E-5
50-54	0.001042532087204472	2.738660449830732E-4	3.4520930039883175E-4	3.981413931266527E-4	1.8411162687937695E-4	1.7030325486342367E-4
55-59	0.0010816558079163399	2.8997581233501874E-4	3.866344164466916E-4	4.1194976514260595E-4	2.1172837091128352E-4	2.0482418490330687E-4
60-64	0.0011000669706042773	3.2219534703890965E-4	4.257581371585592E-4	4.4877209051848133E-4	2.5085209162315115E-4	2.3474232427120567E-4
65-69	0.001127683714636184	3.4520930039883175E-4	4.786902298863801E-4	4.901972065663411E-4	2.738660449830732E-4	2.554548822951355E-4
70-74	0.0013509190622274284	3.567162770787929E-4	4.971013925743177E-4	5.270195319422166E-4	2.8997581233501874E-4	2.876744169990265E-4
75-79	0.00449462509119279	3.751274397667306E-4	5.132111599262633E-4	5.592390666461076E-4	3.1989395170291745E-4	3.314009283828786E-4
80-84	0.0059951348502597125	4.050455791346293E-4	5.247181366062244E-4	6.213767407178973E-4	3.981413931266527E-4	3.452093003988318E-4
85-89	0.0060365599663075725	4.5797767186245015E-4	5.454306946301542E-4	6.581990660937726E-4	5.224167412702322E-4	3.452093003988318E-4
90-94	0.006094094849707377	4.6027906719844236E-4	5.891572060140063E-4	7.203367401655625E-4	5.293209272782088E-4	3.452093003988318E-4
95-99	0.006135519965755237	4.786902298863801E-4	6.029655780299595E-4	7.640632515494144E-4	5.293209272782088E-4	3.452093003988318E-4
100-104	0.00621146601184298	4.8329302055836456E-4	6.098697640379362E-4	8.077897629332664E-4	5.293209272782088E-4	3.452093003988318E-4
105-109	0.006245986941882863	4.8789581123034897E-4	6.190753453819051E-4	8.607218556610874E-4	5.293209272782088E-4	3.452093003988318E-4
110-114	0.006259795313898817	5.10909764590271E-4	6.420892987418273E-4	8.814344136850174E-4	5.293209272782088E-4	3.452093003988318E-4
115-119	0.006298919034610684	5.408279039581698E-4	6.743088334457181E-4	9.159553437249003E-4	5.362251132861854E-4	3.452093003988318E-4
120-124	0.0063748650806984275	5.615404619820998E-4	6.95021391469648E-4	9.366679017488303E-4	5.707460433260687E-4	3.452093003988318E-4
125-129	0.006441605545442201	5.822530200060296E-4	7.203367401655623E-4	9.619832504447447E-4	5.914586013499984E-4	3.567162770787929E-4
130-134	0.006487633452162046	6.029655780299596E-4	7.686660422213988E-4	9.87298599140659E-4	6.305823220618662E-4	3.6131906775077735E-4
135-139	0.0065658808935857805	6.351851127338506E-4	8.008855769252898E-4	0.0010149153431725656	6.697060427737338E-4	3.9123720711867606E-4
140-144	0.006634922753665546	6.651032521017492E-4	8.58420460325095E-4	0.0010517376685484406	6.789116241177025E-4	4.3496371850252805E-4
145-149	0.006674046474377415	6.927199961336559E-4	8.745302276770407E-4	0.0010770530172443554	6.789116241177025E-4	4.3726511383852037E-4
150-154	0.006694759032401344	7.019255774776247E-4	8.90639995028986E-4	0.0011299851099721761	6.973227868056403E-4	4.3726511383852037E-4
155-159	0.006789116241177026	7.157339494935778E-4	9.11352553052916E-4	0.0011760130166920204	7.019255774776247E-4	4.3726511383852037E-4
160-164	0.006828239961888893	7.364465075175078E-4	9.665860411167291E-4	0.0012197395280758724	7.410492981894923E-4	4.418679045105047E-4
165-167	0.006854322442363472	7.441178253041486E-4	9.7809301779669E-4	0.0012427534814357945	7.517891430907893E-4	4.526077494118017E-4
>>END_MODULE
