##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293652
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7873168
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.53985790726173	39.0	39.0	39.0	38.0	39.0
2	40.25292042542468	41.0	40.0	41.0	38.0	41.0
3	40.42370339360217	41.0	40.0	41.0	39.0	41.0
4	40.45803544392803	41.0	40.0	41.0	39.0	41.0
5	40.47270425323072	41.0	40.0	41.0	39.0	41.0
6	40.4618878956984	41.0	40.0	41.0	39.0	41.0
7	40.479847883342515	41.0	40.0	41.0	39.0	41.0
8	40.45653338020985	41.0	40.0	41.0	39.0	41.0
9	40.449498473803686	41.0	40.0	41.0	39.0	41.0
10-14	40.41246631597344	41.0	40.0	41.0	39.0	41.0
15-19	40.316744949428234	41.0	40.0	41.0	39.0	41.0
20-24	40.15621528716268	41.0	40.0	41.0	38.0	41.0
25-29	39.89753326742171	41.0	40.0	41.0	37.6	41.0
30-34	39.50663760763139	40.6	39.0	41.0	36.0	41.0
35-39	39.05191633151991	40.0	37.6	41.0	35.0	41.0
40-44	38.34280734260973	39.4	36.2	41.0	35.0	41.0
45-49	37.57531011658839	38.2	35.0	40.4	34.0	41.0
50-54	36.79504418043664	36.6	35.0	39.6	33.4	41.0
55-59	36.05485222721019	35.2	35.0	38.6	33.0	40.4
60-64	35.23874674082911	34.8	33.6	37.0	31.8	39.8
65-69	34.89556331072829	35.0	33.8	36.0	31.0	39.2
70-74	34.85498703952463	35.0	33.6	36.0	31.0	40.0
75-79	34.82592036649034	35.0	33.0	36.4	30.8	40.6
80-84	35.120437922828515	35.0	33.0	38.8	30.0	41.0
85-89	36.16084600252402	36.0	34.0	40.2	30.4	41.0
90-94	39.79889991242259	40.8	39.8	41.0	37.8	41.0
95-99	39.79658551645649	41.0	40.0	41.0	37.2	41.0
100-104	39.57481969978178	41.0	39.2	41.0	36.2	41.0
105-109	39.2940552497794	40.4	38.8	41.0	35.0	41.0
110-114	38.992997158943446	40.0	38.0	41.0	35.0	41.0
115-119	38.60567766957324	40.0	37.2	41.0	34.4	41.0
120-124	38.22520439207166	39.8	35.8	41.0	34.0	41.0
125-129	37.53702948143557	38.4	35.0	40.4	33.4	41.0
130-134	37.18705948995065	37.6	35.0	40.0	33.0	41.0
135-139	36.60074008678908	36.4	35.0	39.8	33.0	41.0
140-144	35.969392912630255	35.0	34.6	38.8	32.6	40.8
145-149	35.35351517844352	35.0	34.0	37.2	32.0	39.8
150-154	34.766638857787086	35.0	34.0	36.0	31.0	38.8
155-159	34.23806951639173	35.0	33.2	35.0	31.0	37.0
160-164	33.78057554899163	34.6	33.0	35.0	30.4	36.0
165-169	33.38534083032421	34.0	32.8	35.0	30.0	35.2
170-174	33.052678316995255	34.0	32.0	35.0	29.2	35.0
175-178	31.70828801998668	32.5	29.75	34.25	27.0	34.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	3.0
30	71.0
31	648.0
32	4755.0
33	25630.0
34	114127.0
35	473885.0
36	1709788.0
37	2960572.0
38	2191000.0
39	390373.0
40	2316.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.768560254271218	26.187984303142013	27.390778400765743	23.65267704182103
2	24.073371735494533	26.53722110337287	26.8256183533744	22.563788807758197
3	22.315019824294364	25.925091399040383	27.84378537330843	23.91610340335682
4	22.814208968994436	25.261292531799146	28.189554700217244	23.734943798989175
5	23.707851782154275	25.703363626941535	26.593678681821597	23.995105909082596
6	23.743491819303234	26.079857561784532	26.523211495042403	23.65343912386983
7	23.59823898080163	26.169110071066694	26.36716249418277	23.865488453948906
8	23.820792341786685	26.24028853442477	26.127589300774478	23.811329823014066
9	23.91192465345589	26.421625957937135	26.140531994236625	23.525917394370346
10-14	23.786224300053043	26.319275798509572	26.152458578300376	23.742041323137013
15-19	23.796530189626335	26.25014225531578	26.22919770034121	23.724129854716676
20-24	23.803965570149145	26.14572685353596	26.235528061893255	23.81477951442164
25-29	23.790756147969915	26.14236099115375	26.135248225364936	23.931634635511397
30-34	23.80940683597759	26.192155432222453	26.162962609206357	23.8354751225936
35-39	23.77675670073343	26.18433393012825	26.152984414914048	23.885924954224272
40-44	23.765889410717516	26.174718994945874	26.130914518780752	23.92847707555586
45-49	23.910949188433424	26.152788813854855	26.041047263312556	23.895214734399165
50-54	23.91589256065665	26.14718243024917	26.050088096684842	23.886836912409336
55-59	23.838259262345222	26.080609482739348	26.06217217770534	24.018959077210088
60-64	23.800277093032946	26.102605710941262	26.160000650310018	23.937116545715778
65-69	23.754879865385828	26.042665417529516	26.181704747059886	24.020749970024774
70-74	23.650235838991367	26.007723955591956	26.251884882934036	24.09015532248264
75-79	23.534155501317894	25.963177719565998	26.342854617099498	24.15981216201661
80-84	23.38038512578418	25.86270228197849	26.502884226527364	24.254028365709964
85-89	22.51392069875811	25.949485645422527	26.889115029680553	24.647478626138806
90-94	23.26545908362649	26.11486656439458	26.90657754529707	23.713096806681865
95-99	23.53263829726295	26.333145265068787	26.33586950353412	23.798346934134145
100-104	23.56630907999447	26.32203720111077	26.286718141563153	23.824935577331605
105-109	23.582889536806736	26.29208811834105	26.29496372573629	23.830058619115917
110-114	23.628287364827173	26.21051161339406	26.254990904001822	23.906210117776943
115-119	23.66217343527784	26.26927128862385	26.19039891160437	23.878156364493943
120-124	23.658606705950987	26.272739231418303	26.191732202323713	23.876921860306997
125-129	23.676880707393888	26.298994492683786	26.179728675551562	23.844396124370764
130-134	23.737666736205814	26.317400218877218	26.129729386148735	23.81520365876823
135-139	23.742053202841984	26.341001550148878	26.131008056016906	23.785937190992225
140-144	23.67842937189897	26.420224350047206	26.175554171437295	23.72579210661653
145-149	23.61238040944573	26.505313374574648	26.21823378001081	23.664072435968812
150-154	23.515810247273407	26.639991314085037	26.26498318632708	23.579215252314484
155-159	23.40827928968902	26.73773627231174	26.34322382478623	23.51076061321302
160-164	23.290430824397284	26.926637460589735	26.417447224240764	23.36548449077222
165-169	23.14841601676102	27.050659388469985	26.522014996152677	23.27890959861632
170-174	22.91853213517016	27.323549824858606	26.646921592955973	23.11099644701526
175-178	22.82327388337058	27.401415145734948	26.62934033907319	23.145970631821278
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	3.0
14	9.0
15	21.5
16	44.0
17	92.5
18	191.5
19	390.5
20	778.0
21	1446.5
22	2567.0
23	4459.5
24	7479.5
25	12032.5
26	18439.5
27	27317.5
28	38477.5
29	51806.5
30	68025.5
31	86815.5
32	107107.5
33	127567.0
34	147810.0
35	168054.0
36	187438.5
37	206187.0
38	223540.0
39	238031.5
40	251060.0
41	262385.5
42	271764.5
43	279529.5
44	284714.5
45	287996.5
46	289568.0
47	287784.5
48	282943.0
49	276336.5
50	268476.5
51	258414.5
52	246863.5
53	234960.0
54	222910.0
55	211428.5
56	200776.0
57	190385.0
58	180528.5
59	170993.0
60	160530.5
61	150440.0
62	139500.0
63	126885.0
64	114011.5
65	100612.0
66	86894.5
67	73220.5
68	60034.5
69	48003.5
70	37270.5
71	28106.0
72	20589.0
73	14671.0
74	10079.5
75	6608.0
76	4252.5
77	2736.0
78	1625.5
79	915.0
80	516.5
81	275.0
82	143.5
83	72.5
84	36.0
85	20.5
86	10.0
87	4.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1009.0
92-93	2404.0
94-95	4020.0
96-97	5611.0
98-99	7089.0
100-101	9144.0
102-103	11285.0
104-105	13583.0
106-107	16272.0
108-109	19331.0
110-111	23348.0
112-113	25563.0
114-115	28143.0
116-117	31584.0
118-119	35858.0
120-121	40318.0
122-123	45439.0
124-125	50734.0
126-127	56859.0
128-129	63707.0
130-131	71289.0
132-133	90406.0
134-135	118315.0
136-137	127081.0
138-139	130764.0
140-141	137764.0
142-143	141686.0
144-145	152553.0
146-147	166338.0
148-149	172056.0
150-151	184884.0
152-153	191718.0
154-155	199681.0
156-157	214171.0
158-159	234644.0
160-161	250302.0
162-163	266123.0
164-165	293217.0
166-167	327251.0
168-169	343284.0
170-171	371869.0
172-173	400205.0
174-175	564445.0
176-177	1912084.0
178-179	319737.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.32044511286175
#Duplication Level	Percentage of total
1	94.8058216209093
2	4.769517549682091
3	0.3310674732309822
4	0.06011448235446183
5	0.016405878635219265
6	0.002063744803755662
7	0.0010384044880835535
8	0.0010449636675551321
9	0.0010515499336425592
>10	0.011874332294932309
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.540273496005674E-4	0.0	0.0	1.270136748002837E-5	0.0	0.0
2	8.636929886419292E-4	0.0	0.0	2.540273496005674E-5	0.0	0.0
3	8.636929886419292E-4	0.0	1.270136748002837E-5	6.350683740014185E-5	0.0	0.0
4	8.763943561219575E-4	0.0	1.270136748002837E-5	7.620820488017022E-5	0.0	0.0
5	8.763943561219575E-4	0.0	1.270136748002837E-5	8.890957236019858E-5	0.0	0.0
6	8.763943561219575E-4	0.0	1.270136748002837E-5	1.0161093984022696E-4	0.0	0.0
7	8.763943561219575E-4	1.270136748002837E-5	3.810410244008511E-5	1.1431230732025533E-4	0.0	0.0
8	8.763943561219575E-4	1.270136748002837E-5	3.810410244008511E-5	1.270136748002837E-4	0.0	0.0
9	8.763943561219575E-4	1.270136748002837E-5	3.810410244008511E-5	1.5241640976034043E-4	0.0	0.0
10-14	8.890957236019857E-4	3.556382894407944E-5	4.318464943209645E-5	1.6765805073637447E-4	0.0	0.0
15-19	8.890957236019857E-4	5.8426290408130506E-5	9.653039284821563E-5	2.083024266724653E-4	0.0	0.0
20-24	9.170387320580483E-4	8.128875187218156E-5	1.4479558927232343E-4	2.514870761045617E-4	0.0	0.0
25-29	9.27199826042071E-4	1.0923176032824398E-4	1.8035941821640283E-4	3.073730930166866E-4	7.6208204880170226E-6	0.0
30-34	9.399011935220995E-4	1.3209422179229503E-4	2.1338297366447662E-4	3.302355544807376E-4	2.540273496005674E-5	0.0
35-39	9.653039284821561E-4	1.4225531577631773E-4	2.464065291125504E-4	3.632591099288114E-4	8.128875187218156E-5	5.080546992011348E-6
40-44	9.780052959621846E-4	1.7019832423238016E-4	2.540273496005674E-4	3.785007509048454E-4	1.2193312780827235E-4	1.778191447203972E-5
45-49	9.90706663442213E-4	2.1338297366447662E-4	2.616481700885844E-4	4.0644375936090785E-4	1.4987613626433476E-4	3.556382894407944E-5
50-54	9.90706663442213E-4	2.3878570862453336E-4	3.0483281952068086E-4	4.3946731480898164E-4	1.8035941821640283E-4	9.144984585620425E-5
55-59	0.0010161093984022696	2.438662556165447E-4	3.3785637496875465E-4	5.080546992011347E-4	2.0068160618444826E-4	1.1685258081626101E-4
60-64	0.0010389718598663206	2.616481700885844E-4	3.6325910992881133E-4	5.359977076571972E-4	2.540273496005674E-4	1.5241640976034043E-4
65-69	0.0010491329538503433	3.0483281952068086E-4	4.039034858649022E-4	5.639407161132596E-4	3.2769528098473193E-4	1.549566832563461E-4
70-74	0.0011888479961306554	3.0483281952068086E-4	4.2930622082495886E-4	6.376086474974242E-4	3.708799304168284E-4	1.9560105919243692E-4
75-79	0.0038256518849845446	3.0483281952068086E-4	4.343867678169702E-4	6.706322029454979E-4	4.166048533449306E-4	2.2862461464051063E-4
80-84	0.004585193660290242	3.226147339927206E-4	4.4454786180099287E-4	7.061960318895773E-4	4.4454786180099287E-4	2.2862461464051063E-4
85-89	0.004625838036226333	3.42936921960766E-4	4.5470895578501565E-4	7.544612283136851E-4	4.928130582251007E-4	2.2862461464051063E-4
90-94	0.0046715629591544345	3.42936921960766E-4	4.6995059676104966E-4	7.976458777457816E-4	4.953533317211064E-4	2.2862461464051063E-4
95-99	0.00470966706159452	3.6071883643280567E-4	4.928130582251008E-4	8.382902536818724E-4	4.953533317211064E-4	2.362454351285277E-4
100-104	0.004745230890538599	3.7342020391283406E-4	5.309171606651859E-4	8.992568175860086E-4	5.131352461931461E-4	2.4132598212053903E-4
105-109	0.0047604725315146335	3.8612157139286245E-4	5.512393486332313E-4	9.11958185066037E-4	5.436185281452142E-4	2.4132598212053903E-4
110-114	0.004783334992978684	3.988229388728908E-4	5.969642715613334E-4	9.19579005554054E-4	5.563198956252426E-4	2.4132598212053903E-4
115-119	0.004801116907450724	4.1406457984892486E-4	5.99504545057339E-4	9.805455694581902E-4	5.715615366012766E-4	2.4132598212053903E-4
120-124	0.00484430155688282	4.623297762730327E-4	6.249072800173958E-4	0.0010110288514102585	6.07125365545356E-4	2.4894680260855606E-4
125-129	0.004923050035258996	4.953533317211064E-4	6.350683740014185E-4	0.0010465926803543376	6.147461860333732E-4	2.540273496005674E-4
130-134	0.004978936052171121	5.080546992011347E-4	6.503100149774525E-4	0.001059294047834366	6.274475535134015E-4	2.540273496005674E-4
135-139	0.005052603983555285	5.283768871691801E-4	6.630113824574809E-4	0.0010923176032824398	6.350683740014185E-4	2.6164817008858445E-4
140-144	0.005215181487299649	5.461588016412199E-4	6.909543909135433E-4	0.0011278814322265192	6.401489209934298E-4	2.667287170805958E-4
145-149	0.005321872974131886	5.614004426172539E-4	7.290584933536284E-4	0.0011812271756426386	6.477697414814469E-4	2.7943008456062413E-4
150-154	0.005405701999500074	5.84262904081305E-4	7.671625957937136E-4	0.0012040896371066894	6.477697414814469E-4	2.921314520406525E-4
155-159	0.00576642083593288	5.84262904081305E-4	8.154277922178214E-4	0.0012498145600347917	6.579308354654695E-4	2.921314520406525E-4
160-164	0.00616016322781376	5.84262904081305E-4	8.382902536818724E-4	0.001270136748002837	6.706322029454979E-4	2.921314520406525E-4
165-167	0.0065623731980146574	5.84262904081305E-4	8.382902536818723E-4	0.0012828381154828654	6.731724764415036E-4	2.921314520406525E-4
>>END_MODULE
