##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293655
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11640070
Total Bases	1.9 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.418733306586645	39.0	39.0	39.0	37.0	39.0
2	40.19357203178331	41.0	40.0	41.0	38.0	41.0
3	40.22082762388886	41.0	40.0	41.0	38.0	41.0
4	40.23204817496802	41.0	40.0	41.0	38.0	41.0
5	40.23280323915578	41.0	40.0	41.0	38.0	41.0
6	40.21146316130401	41.0	40.0	41.0	38.0	41.0
7	40.234022733540264	41.0	40.0	41.0	38.0	41.0
8	40.21954971061171	41.0	40.0	41.0	38.0	41.0
9	40.20639901650076	41.0	40.0	41.0	38.0	41.0
10-14	40.14402925411961	41.0	40.0	41.0	38.0	41.0
15-19	40.03201925761614	41.0	40.0	41.0	38.0	41.0
20-24	39.86762246275151	41.0	40.0	41.0	38.0	41.0
25-29	39.643121252707246	40.8	39.4	41.0	37.0	41.0
30-34	39.470781601828854	40.4	39.0	41.0	36.0	41.0
35-39	39.38855264616106	41.0	39.0	41.0	35.2	41.0
40-44	38.96424136624607	40.0	37.4	41.0	35.0	41.0
45-49	38.3257012543739	39.4	36.0	41.0	35.0	41.0
50-54	37.61873461242071	38.0	35.0	40.6	34.8	41.0
55-59	36.888767627686086	36.4	35.0	39.4	34.0	41.0
60-64	36.07649452279926	35.4	34.8	38.0	33.6	40.2
65-69	35.65884705160708	35.0	35.0	36.6	33.8	39.0
70-74	35.38085226291595	35.0	35.0	36.0	34.0	38.0
75-79	35.16137765494537	35.0	35.0	35.4	34.0	37.0
80-84	35.06791033043616	35.0	35.0	35.0	33.4	36.6
85-89	34.875473858834184	35.0	34.6	35.0	32.6	36.2
90-94	39.66648648039446	40.6	39.8	40.6	37.8	40.6
95-99	39.957097192540644	41.0	40.0	41.0	38.0	41.0
100-104	39.84569394762457	41.0	40.0	41.0	37.4	41.0
105-109	39.69846440553333	41.0	40.0	41.0	36.8	41.0
110-114	39.523069570306134	40.8	39.0	41.0	36.0	41.0
115-119	39.25903612696648	40.0	38.6	41.0	35.0	41.0
120-124	39.01998203771181	40.0	38.0	41.0	35.0	41.0
125-129	38.53409831925025	39.6	37.2	40.6	34.6	41.0
130-134	38.27445569968434	39.6	36.0	41.0	34.4	41.0
135-139	37.92864307999968	39.0	35.0	41.0	34.8	41.0
140-144	37.327155472744835	37.2	35.0	40.4	34.0	41.0
145-149	36.60347421708126	35.8	35.0	39.0	34.0	41.0
150-154	35.91768184983407	35.0	35.0	37.0	34.0	39.4
155-159	35.36550425303868	35.0	35.0	36.0	34.0	38.0
160-164	34.98111881563248	35.0	35.0	35.4	33.4	36.6
165-169	34.74348546844275	35.0	35.0	35.0	33.0	36.0
170-174	34.61114416500998	35.0	35.0	35.0	33.0	36.0
175-178	34.30347136832616	35.0	34.5	35.0	32.25	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	120.0
30	909.0
31	5465.0
32	25202.0
33	88308.0
34	237930.0
35	568156.0
36	1502273.0
37	3548937.0
38	4313584.0
39	1331260.0
40	17923.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.83894083111184	27.326888927643907	28.61012863324705	22.2240416079972
2	22.953487393117054	27.708390069819167	27.94428212201473	21.393840415049052
3	20.930209182590826	26.967320643260734	29.338363085445362	22.764107088703074
4	21.63430288649467	26.196612219685967	29.602321979163353	22.56676291465601
5	22.56193476499712	26.81320644978939	27.87887873526534	22.74598004994815
6	22.431823863602197	27.138505180810768	27.85973795690232	22.569932998684717
7	22.495509047626	27.218332879441448	27.58615712792105	22.7000009450115
8	22.623051235946175	27.38925968658264	27.32003329876882	22.66765577870236
9	22.649099189266046	27.52548738968065	27.41199150864213	22.41342191241118
10-14	22.63805286394326	27.419998333343354	27.293581567808438	22.648367234904946
15-19	22.61359252994183	27.414237199604468	27.38181127776723	22.590358992686472
20-24	22.581448393351586	27.303304877032524	27.444757634619037	22.670489094996853
25-29	22.60526783773637	27.290358219495243	27.312431969910833	22.79194197285755
30-34	22.630145695000117	27.322041877755034	27.32892499787372	22.71888742937113
35-39	22.65819535449529	27.32948685016499	27.29322589984424	22.719091895495474
40-44	22.674513125780173	27.299117616990277	27.266140152078123	22.76022910515143
45-49	22.774186065891357	27.276074800237453	27.216099215898186	22.733639917973004
50-54	22.749081405867834	27.3263580030017	27.217535633376777	22.707024957753692
55-59	22.72699734623589	27.253985585997338	27.218293360778755	22.800723706988016
60-64	22.701932204875057	27.304909678378227	27.26488414588572	22.728273970861
65-69	22.703617761748855	27.291050655193654	27.255842963143696	22.749488619913798
70-74	22.658856862544642	27.275921880194883	27.255434030895003	22.809787226365476
75-79	22.639607837409912	27.296335846777552	27.28536340417197	22.778692911640565
80-84	22.639764193857943	27.311586614169848	27.25389452125288	22.794754670719332
85-89	22.62673849899528	27.28343214430841	27.231092252881638	22.85873710381467
90-94	21.98365122540885	27.084838915858917	28.552807869952453	22.378701988779788
95-99	22.451166269748363	27.416647542960487	27.510840574471718	22.62134561281943
100-104	22.5751586340472	27.459918814633916	27.33579675644754	22.629125794871353
105-109	22.49532504089084	27.424374584969136	27.40294227587194	22.677358098268087
110-114	22.518480324141983	27.32402164000626	27.45387642851218	22.70362160733957
115-119	22.550609566706616	27.38195223690414	27.31479283636072	22.75264536002853
120-124	22.52362676351393	27.374266687826548	27.30415278049338	22.797953768166142
125-129	22.504152584253546	27.388628937005667	27.361819939912547	22.745398538828237
130-134	22.612629371670664	27.379383528164624	27.257441268072835	22.750545832091873
135-139	22.67502358524532	27.335392470161	27.213140401118626	22.776443543475054
140-144	22.66213350659746	27.391345668258378	27.276584049417185	22.669936775726985
145-149	22.63225845576054	27.424971856385554	27.2666465888876	22.67612309896631
150-154	22.56730560965944	27.468693404617472	27.295445980142834	22.668555005580252
155-159	22.535991109992807	27.48017196435678	27.360196326000352	22.62364059965006
160-164	22.509352455713874	27.57092860839714	27.35161928700801	22.568099648880978
165-169	22.433033298275618	27.56823103832362	27.407830750820384	22.590904912580378
170-174	22.404045724644654	27.660401615896962	27.448716565708203	22.486836093750185
175-178	22.378592752232528	27.723862658221986	27.38475450245586	22.512790087089623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	1.5
11	7.5
12	17.5
13	33.5
14	67.0
15	148.5
16	321.0
17	657.0
18	1286.5
19	2410.0
20	4187.0
21	7047.5
22	11455.5
23	17454.0
24	25605.5
25	35966.5
26	49153.0
27	64996.0
28	83135.5
29	103555.0
30	126569.5
31	151646.0
32	178548.0
33	208460.5
34	240013.0
35	272613.0
36	307077.0
37	342516.0
38	376769.5
39	408871.5
40	438265.5
41	463649.5
42	484222.0
43	498199.0
44	504711.5
45	504499.0
46	496734.0
47	482512.0
48	462766.0
49	438219.5
50	411597.0
51	382635.5
52	352047.5
53	322318.5
54	293663.5
55	266725.0
56	241975.0
57	218797.5
58	197116.0
59	177133.0
60	158556.5
61	141311.5
62	125096.0
63	109196.5
64	93752.5
65	79092.5
66	65802.5
67	53695.0
68	42331.0
69	32700.0
70	24815.0
71	18180.5
72	12931.0
73	8966.5
74	6025.0
75	4004.5
76	2580.5
77	1553.5
78	894.5
79	506.0
80	291.0
81	153.5
82	75.0
83	33.0
84	14.5
85	8.5
86	5.5
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1329.0
92-93	2878.0
94-95	4230.0
96-97	5483.0
98-99	7714.0
100-101	8077.0
102-103	9479.0
104-105	10482.0
106-107	11673.0
108-109	12844.0
110-111	13633.0
112-113	15080.0
114-115	15150.0
116-117	16169.0
118-119	16983.0
120-121	17756.0
122-123	19237.0
124-125	19341.0
126-127	20310.0
128-129	21109.0
130-131	22295.0
132-133	24586.0
134-135	125118.0
136-137	114974.0
138-139	100461.0
140-141	100817.0
142-143	97295.0
144-145	109744.0
146-147	103017.0
148-149	111234.0
150-151	105687.0
152-153	102346.0
154-155	107188.0
156-157	99066.0
158-159	105198.0
160-161	108991.0
162-163	112373.0
164-165	119117.0
166-167	118992.0
168-169	116635.0
170-171	122502.0
172-173	129836.0
174-175	130446.0
176-177	371195.0
178-179	8632000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.13968014647682
#Duplication Level	Percentage of total
1	88.96417665357018
2	9.27251730612553
3	1.314519972242692
4	0.29157432932162713
5	0.09455929884702147
6	0.03778198864543297
7	0.012306184639690807
8	0.006179449969361346
9	0.0
>10	0.006384816638480977
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	7.731912265132426E-5	8.591013627924918E-6	0.0	0.0	0.0	0.0
2	3.9518662688454623E-4	8.591013627924918E-6	8.591013627924918E-6	8.591013627924918E-6	0.0	0.0
3	4.0377764051247115E-4	4.295506813962459E-5	2.5773040883774755E-5	8.591013627924918E-6	8.591013627924918E-6	0.0
4	4.0377764051247115E-4	5.154608176754951E-5	2.5773040883774755E-5	1.7182027255849835E-5	8.591013627924918E-6	0.0
5	4.0377764051247115E-4	5.154608176754951E-5	3.436405451169967E-5	1.7182027255849835E-5	8.591013627924918E-6	0.0
6	4.0377764051247115E-4	5.154608176754951E-5	3.436405451169967E-5	3.436405451169967E-5	8.591013627924918E-6	0.0
7	4.1236865414039607E-4	5.154608176754951E-5	3.436405451169967E-5	4.295506813962459E-5	1.7182027255849835E-5	0.0
8	4.20959667768321E-4	5.154608176754951E-5	4.295506813962459E-5	6.013709539547443E-5	1.7182027255849835E-5	0.0
9	4.20959667768321E-4	5.154608176754951E-5	4.295506813962459E-5	6.013709539547443E-5	2.5773040883774755E-5	0.0
10-14	4.467327086520958E-4	5.6700689944304457E-5	5.15460817675495E-5	7.731912265132426E-5	2.577304088377475E-5	0.0
15-19	4.639147359079456E-4	6.013709539547442E-5	6.529170357222938E-5	1.254287989677038E-4	2.920944633494472E-5	0.0
20-24	4.759421549870405E-4	6.357350084664439E-5	1.2371059624211882E-4	1.6151105620498845E-4	3.436405451169967E-5	0.0
25-29	4.8109676316379537E-4	7.731912265132426E-5	1.563564480282335E-4	1.7697488073525333E-4	3.9518662688454625E-5	0.0
30-34	5.395156558336848E-4	8.591013627924918E-5	1.7010206983291337E-4	1.890022998143482E-4	6.185529812105941E-5	5.15460817675495E-6
35-39	7.027449147642583E-4	9.621935263275909E-5	1.7525667800966834E-4	2.11338935246953E-4	9.965575808392904E-5	2.2336635432604786E-5
40-44	8.024006728481874E-4	1.0824677171185396E-4	1.8556589436317825E-4	2.302391652283878E-4	1.357380153212137E-4	5.326428449313449E-5
45-49	9.312658772670611E-4	1.2027419079094884E-4	2.0790252979578303E-4	2.594486115633325E-4	1.8556589436317825E-4	9.965575808392904E-5
50-54	0.0010618492844115199	1.2027419079094884E-4	2.4398478703306766E-4	2.886580578982772E-4	2.3883017885631272E-4	1.2371059624211882E-4
55-59	0.001173532461574544	1.2027419079094884E-4	2.749124360935973E-4	3.419223423914117E-4	2.7834884154476734E-4	1.3230160987004374E-4
60-64	0.001271470016932888	1.3917442077238367E-4	2.920944633494472E-4	3.591043696472616E-4	3.2130390968439194E-4	1.7525667800966834E-4
65-69	0.0013436345314074571	1.5463824530264852E-4	2.955308688006171E-4	3.780045996286964E-4	3.625407750984315E-4	2.0790252979578303E-4
70-74	0.002010297188934431	1.5463824530264852E-4	3.1271289605646696E-4	4.1580505959156605E-4	4.054958432380561E-4	2.2852096250280284E-4
75-79	0.014226718567843665	1.6322925893057344E-4	3.2817672058673184E-4	4.4501450592651073E-4	4.7937856043821036E-4	2.3367557067955776E-4
80-84	0.019250743337454156	1.872840970887632E-4	3.6082257237284656E-4	4.639147359079456E-4	6.082437648570842E-4	2.4054838158189769E-4
85-89	0.01927307997288676	2.0274792161902806E-4	3.969048296101312E-4	5.034333985964002E-4	8.144280919272823E-4	2.491393952098226E-4
90-94	0.019324626054654313	2.0790252979578303E-4	4.2439607321949097E-4	5.2405183130342E-4	8.316101191831321E-4	2.491393952098226E-4
95-99	0.01934524448736133	2.2164815160046289E-4	4.347052895730009E-4	5.395156558336848E-4	8.333283219087171E-4	2.491393952098226E-4
100-104	0.019381326744598616	2.2336635432604785E-4	4.4501450592651073E-4	5.773161157965544E-4	8.436375382622268E-4	2.5601220611216256E-4
105-109	0.019407099785482388	2.2852096250280284E-4	4.7594215498704043E-4	6.013709539547442E-4	8.608195655180768E-4	2.577304088377475E-4
110-114	0.019417409001835896	2.319573679539728E-4	5.15460817675495E-4	6.168347784850091E-4	8.711287818715868E-4	2.577304088377475E-4
115-119	0.019432872826366163	2.319573679539728E-4	5.274882367545899E-4	6.340168057408588E-4	8.969018227553615E-4	2.577304088377475E-4
120-124	0.019532528584450093	2.4054838158189769E-4	5.566976830895347E-4	6.529170357222938E-4	9.209566609135512E-4	2.663214224656725E-4
125-129	0.019776513371483163	2.4398478703306766E-4	5.755979130709695E-4	6.804082793316535E-4	9.707845399555158E-4	2.783488415447673E-4
130-134	0.019984415901278946	2.611668142889175E-4	5.876253321500643E-4	7.061813202154282E-4	0.0010051485944672154	2.955308688006171E-4
135-139	0.02020606405287941	2.852216524471073E-4	5.996527512291593E-4	7.37108969275958E-4	0.0010240488244486503	3.19585706958807E-4
140-144	0.020398502758144928	3.006854769773721E-4	6.28862197564104E-4	7.680366183364876E-4	0.00103435804080216	3.350495314890718E-4
145-149	0.02051877694893587	3.0584008515412706E-4	6.443260220943688E-4	7.852186455923376E-4	0.0010755949062161998	3.4020413966582674E-4
150-154	0.020608123490666295	3.0927649060529704E-4	6.494806302711238E-4	8.006824701226025E-4	0.0011185499743558243	3.505133560193366E-4
155-159	0.020704342843299053	3.1271289605646696E-4	6.529170357222938E-4	8.247373082807923E-4	0.0011340137988860891	3.694135860007715E-4
160-164	0.020860699291327285	3.1786750423322196E-4	6.529170357222938E-4	8.35046524634302E-4	0.0011426048125140142	3.694135860007715E-4
165-167	0.020987846293020574	3.1786750423322196E-4	6.615080493502187E-4	8.533740203738753E-4	0.0011511958261419392	3.7227725721007975E-4
>>END_MODULE
