##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293657
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11672464
Total Bases	2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.405422111389676	39.0	39.0	39.0	37.0	39.0
2	40.187070099338065	41.0	40.0	41.0	38.0	41.0
3	40.221881258318724	41.0	40.0	41.0	38.0	41.0
4	40.22755486759265	41.0	40.0	41.0	38.0	41.0
5	40.22654239927405	41.0	40.0	41.0	38.0	41.0
6	40.214513405224466	41.0	40.0	41.0	38.0	41.0
7	40.23899332651615	41.0	40.0	41.0	38.0	41.0
8	40.22750183680155	41.0	40.0	41.0	38.0	41.0
9	40.212367671470226	41.0	40.0	41.0	38.0	41.0
10-14	40.15459140417996	41.0	40.0	41.0	38.0	41.0
15-19	40.0429193184918	41.0	40.0	41.0	38.0	41.0
20-24	39.88240750196359	41.0	40.0	41.0	38.0	41.0
25-29	39.65625444636197	40.8	39.4	41.0	37.0	41.0
30-34	39.489196745434384	40.6	39.0	41.0	36.0	41.0
35-39	39.40065850706415	41.0	39.0	41.0	35.2	41.0
40-44	38.97493243928617	40.0	37.4	41.0	35.0	41.0
45-49	38.33697807078265	39.4	36.0	41.0	35.0	41.0
50-54	37.617619450357694	38.0	35.0	40.6	35.0	41.0
55-59	36.87693405608276	36.4	35.0	39.2	34.0	41.0
60-64	36.07877413029503	35.2	34.8	38.0	33.6	40.2
65-69	35.654753872018794	35.0	35.0	36.6	33.8	39.0
70-74	35.380632127029905	35.0	35.0	36.0	34.0	37.8
75-79	35.16502537938862	35.0	35.0	35.2	34.0	37.0
80-84	35.07169163254648	35.0	35.0	35.0	33.8	36.4
85-89	34.87813856611594	35.0	34.6	35.0	32.6	36.2
90-94	39.71850254309685	40.6	39.8	40.6	37.8	40.6
95-99	39.997646227711485	41.0	40.0	41.0	38.0	41.0
100-104	39.89081596640413	41.0	40.0	41.0	37.8	41.0
105-109	39.75148117620586	41.0	40.0	41.0	37.0	41.0
110-114	39.576589142879584	41.0	39.2	41.0	36.2	41.0
115-119	39.32226858758749	40.2	38.8	41.0	35.2	41.0
120-124	39.09066857371009	40.0	38.0	41.0	35.0	41.0
125-129	38.61165367678849	39.6	37.6	40.6	34.6	41.0
130-134	38.36397336062461	39.8	36.4	41.0	34.8	41.0
135-139	38.01860966202624	39.0	35.0	41.0	34.8	41.0
140-144	37.41669269071809	37.2	35.0	40.6	34.6	41.0
145-149	36.69604593772017	36.2	35.0	39.0	34.0	41.0
150-154	36.01269026945717	35.0	35.0	37.2	34.0	39.8
155-159	35.44350023333443	35.0	35.0	36.2	34.0	38.2
160-164	35.04208027798698	35.0	35.0	35.4	34.0	36.8
165-169	34.784201743321916	35.0	35.0	35.0	33.0	36.0
170-174	34.64592359005286	35.0	35.0	35.0	33.0	36.0
175-178	34.32869571020348	35.0	34.5	35.0	32.25	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	48.0
30	634.0
31	4607.0
32	23424.0
33	84755.0
34	233376.0
35	550883.0
36	1454999.0
37	3507930.0
38	4370938.0
39	1419624.0
40	21244.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.856096536258327	27.288728412441454	28.614386816699543	22.240788234600682
2	22.979629665167526	27.668751002359055	27.96678576177232	21.384833570701094
3	20.9789466902618	26.91296370672036	29.289985387832424	22.818104215185414
4	21.689893410680043	26.182261089004	29.55226077373209	22.575584726583863
5	22.598793193964873	26.767441732953728	27.855858026205947	22.777907046875452
6	22.464674125360336	27.135341775309822	27.839297683848073	22.560686415481772
7	22.516265631660975	27.20668061173716	27.547354183315537	22.72969957328633
8	22.65826649797335	27.382967298078626	27.286603753928908	22.672162450019123
9	22.684036549609406	27.50471537115043	27.365233253235992	22.446014826004177
10-14	22.668949760736037	27.388308072742824	27.26335587755936	22.67938628896178
15-19	22.639963592948327	27.38582187959629	27.35434951866204	22.619865008793344
20-24	22.609219441584912	27.275533255017965	27.41361378368783	22.70163351970929
25-29	22.62214387639148	27.27426874051614	27.285135340747253	22.81845204234513
30-34	22.668476852873567	27.303926574543304	27.28326769737735	22.74432887520578
35-39	22.684089666072218	27.297271595783034	27.275807404503453	22.742831333641295
40-44	22.695468583154337	27.272577580877527	27.244026625398032	22.787927210570107
45-49	22.78686659474812	27.24111036024613	27.218081803464976	22.753941241540776
50-54	22.77680530863064	27.2946431875909	27.198426998789632	22.730124504988837
55-59	22.748953434339143	27.23214909893918	27.19811686718417	22.82078059953751
60-64	22.725045885770136	27.276638420131345	27.242587340599204	22.755728353499315
65-69	22.71226195257488	27.27475706928717	27.226431368732428	22.786549609405522
70-74	22.684267863237785	27.262584832131413	27.230399682534895	22.8227476220959
75-79	22.67173409144804	27.269890915919724	27.269548229062863	22.78882676356937
80-84	22.664928330470758	27.291814307587497	27.21938744038962	22.823869921552127
85-89	22.657656515368135	27.263645447953404	27.191833703663598	22.886864333014863
90-94	22.01129134812086	27.054541627172608	28.522371985096402	22.41179503961013
95-99	22.4948124098817	27.37267681707991	27.48338427746902	22.64912649556937
100-104	22.609382555941444	27.422889900316527	27.31513358904471	22.652593954697323
105-109	22.532932341436197	27.38359498040282	27.380946747547608	22.702525930613373
110-114	22.558033080467656	27.280676482864223	27.425907661222855	22.735382775445263
115-119	22.586819745585352	27.34743015547016	27.287218801797003	22.778531297147488
120-124	22.572773414151282	27.34172953861403	27.279045484762648	22.806451562472045
125-129	22.556011627271076	27.34429844631176	27.34098684203184	22.758703084385328
130-134	22.653276772124983	27.339860128689985	27.23692852833138	22.769934570853657
135-139	22.70881055893356	27.297461178337663	27.19184409089118	22.801884171837596
140-144	22.690806711602608	27.350106443578582	27.26618798323534	22.692898861583465
145-149	22.66661632147738	27.382446976066387	27.248197273443253	22.702739429012976
150-154	22.614835020486872	27.41612605197215	27.26454830656428	22.704490620976692
155-159	22.584762922719555	27.451356415997946	27.3223467655974	22.641533895685097
160-164	22.55022441426554	27.527401093041114	27.31445269348967	22.607921799203673
165-169	22.473068942192132	27.531135402098972	27.369569783071302	22.626225872637594
170-174	22.454595103220957	27.615995941876086	27.4207935642945	22.508615390608462
175-178	22.439931376973174	27.67544743881565	27.339532088611197	22.545089095599984
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.5
10	2.5
11	5.5
12	15.5
13	29.0
14	72.0
15	166.0
16	356.5
17	698.0
18	1302.5
19	2389.0
20	4247.5
21	7017.0
22	11218.0
23	17281.5
24	25480.0
25	35974.0
26	48785.0
27	64485.5
28	82700.0
29	102992.5
30	125826.5
31	151551.0
32	179014.0
33	207829.5
34	239509.0
35	272736.5
36	306945.5
37	341851.5
38	375472.0
39	408268.0
40	438969.0
41	464131.0
42	483178.0
43	496943.5
44	504531.0
45	504555.5
46	497612.0
47	483675.0
48	463262.0
49	439094.0
50	412201.5
51	384026.0
52	354513.0
53	324213.5
54	295903.0
55	269075.5
56	243966.5
57	220676.0
58	198855.0
59	178926.0
60	160382.0
61	143361.5
62	126866.5
63	110995.5
64	95633.5
65	80698.5
66	67259.0
67	54779.5
68	43453.5
69	33702.5
70	25542.0
71	18724.5
72	13409.0
73	9382.0
74	6224.5
75	4008.5
76	2542.5
77	1584.0
78	937.0
79	565.5
80	320.0
81	172.5
82	88.5
83	41.5
84	21.5
85	14.0
86	6.0
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1155.0
92-93	2555.0
94-95	3741.0
96-97	4821.0
98-99	6126.0
100-101	7283.0
102-103	8568.0
104-105	9756.0
106-107	10872.0
108-109	11913.0
110-111	12805.0
112-113	13552.0
114-115	16359.0
116-117	16388.0
118-119	16172.0
120-121	16548.0
122-123	17456.0
124-125	18043.0
126-127	18942.0
128-129	19531.0
130-131	20320.0
132-133	22516.0
134-135	104879.0
136-137	101616.0
138-139	87277.0
140-141	88321.0
142-143	98587.0
144-145	115032.0
146-147	109994.0
148-149	109836.0
150-151	103161.0
152-153	103882.0
154-155	96209.0
156-157	101207.0
158-159	99384.0
160-161	106523.0
162-163	106197.0
164-165	106803.0
166-167	120371.0
168-169	112555.0
170-171	115610.0
172-173	126440.0
174-175	132009.0
176-177	375705.0
178-179	8775444.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.20426933246236
#Duplication Level	Percentage of total
1	89.07191392821736
2	9.216276705054147
3	1.2862738290939892
4	0.2804299424755589
5	0.08133805222131564
6	0.02961135353119177
7	0.01435628562548081
8	0.010298291025822753
9	0.0020684430857568345
>10	0.007433169669373584
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.0280605705873242E-4	0.0	8.567171421561034E-6	1.713434284312207E-5	0.0	0.0
2	3.426868568624414E-4	1.713434284312207E-5	1.713434284312207E-5	2.5701514264683105E-5	0.0	0.0
3	3.426868568624414E-4	4.283585710780517E-5	1.713434284312207E-5	3.426868568624414E-5	0.0	0.0
4	3.5125402828400243E-4	5.140302852936621E-5	1.713434284312207E-5	4.283585710780517E-5	0.0	0.0
5	3.5125402828400243E-4	5.140302852936621E-5	1.713434284312207E-5	4.283585710780517E-5	0.0	0.0
6	3.5125402828400243E-4	5.9970199950927245E-5	1.713434284312207E-5	5.140302852936621E-5	0.0	0.0
7	3.5982119970556347E-4	5.9970199950927245E-5	1.713434284312207E-5	5.140302852936621E-5	8.567171421561034E-6	0.0
8	3.5982119970556347E-4	5.9970199950927245E-5	1.713434284312207E-5	5.140302852936621E-5	8.567171421561034E-6	0.0
9	3.5982119970556347E-4	6.853737137248827E-5	2.5701514264683105E-5	5.9970199950927245E-5	8.567171421561034E-6	0.0
10-14	3.7181523969574893E-4	6.853737137248827E-5	6.339706851955166E-5	9.766575420579579E-5	2.3988079980370896E-5	0.0
15-19	3.9923018824474416E-4	7.539110850973711E-5	9.766575420579579E-5	1.4050161131360094E-4	3.084181711761973E-5	0.0
20-24	4.0608392538199305E-4	9.766575420579579E-5	1.1137322848029343E-4	1.456419141665376E-4	4.9689594245053994E-5	0.0
25-29	4.317854396466762E-4	1.0280605705873243E-4	1.2165383418616669E-4	1.6448969129397186E-4	5.9970199950927245E-5	0.0
30-34	4.7633473103879354E-4	1.0794635991166904E-4	1.3878817702928876E-4	2.0218524554884042E-4	7.196423994111269E-5	1.7134342843122069E-6
35-39	5.740004852445894E-4	1.1137322848029343E-4	1.713434284312207E-4	2.3302706266646015E-4	1.0280605705873243E-4	3.084181711761972E-5
40-44	6.750931080190096E-4	1.233672684704789E-4	2.0218524554884042E-4	2.5016140550958223E-4	1.439284798822254E-4	4.968959424505401E-5
45-49	7.813260336463663E-4	1.4735534845084977E-4	2.261733255292113E-4	2.8271665691151414E-4	1.936180741272794E-4	7.881797707836151E-5
50-54	8.909858278423475E-4	1.6277625700965965E-4	2.467345369409578E-4	3.101316054605094E-4	2.4844797122526997E-4	1.1822696561754227E-4
55-59	0.0010006456220383287	1.6448969129397186E-4	2.6558231406839205E-4	3.238390797350071E-4	3.084181711761972E-4	1.4050161131360097E-4
60-64	0.001117159153371559	1.7819716556846953E-4	2.8614352548013856E-4	3.289793825879437E-4	3.7181523969574893E-4	1.7819716556846953E-4
65-69	0.0011719890504695496	1.8676433699003054E-4	3.101316054605095E-4	3.7181523969574893E-4	4.1465109680355404E-4	1.970449426959038E-4
70-74	0.0019327538727041693	1.970449426959038E-4	3.358331197251925E-4	4.180779653721785E-4	4.4720634820548605E-4	2.1760615410765027E-4
75-79	0.014903451404947577	2.0389867983315263E-4	3.5639433113693905E-4	4.643406910486081E-4	4.934690738819157E-4	2.3302706266646015E-4
80-84	0.02048924717180537	2.0903898268608925E-4	3.649615025585001E-4	5.020362453034766E-4	6.271169480582678E-4	2.6386887978407985E-4
85-89	0.020509808383217115	2.3816736551939677E-4	3.786689768329977E-4	5.431586681269696E-4	8.515768393031669E-4	2.6558231406839205E-4
90-94	0.020578345754589604	2.3988079980370898E-4	3.9580331967611974E-4	5.620064452544038E-4	8.652843135776645E-4	2.6558231406839205E-4
95-99	0.020610901005991535	2.467345369409578E-4	4.163645310878663E-4	5.859945252347748E-4	8.721380507149132E-4	2.6558231406839205E-4
100-104	0.02065373686309934	2.741494854899531E-4	4.540600853427348E-4	6.288303823425799E-4	8.87558959273723E-4	2.6558231406839205E-4
105-109	0.02067429807451109	2.964241311860118E-4	4.88328771028979E-4	6.768065423033218E-4	8.909858278423475E-4	2.6558231406839205E-4
110-114	0.020717133931618895	2.998509997546362E-4	5.191705881465987E-4	7.230692679797514E-4	9.012664335482208E-4	2.741494854899531E-4
115-119	0.020749689183020826	3.0156443403894843E-4	5.414452338426575E-4	7.4534391367581E-4	9.11547039254094E-4	2.741494854899531E-4
120-124	0.020828507160099187	3.186987768820705E-4	5.551527081171551E-4	7.607648222346198E-4	9.303948163815283E-4	2.7928978834288967E-4
125-129	0.02098100281140297	3.3583311972519255E-4	5.740004852445893E-4	7.933200736365519E-4	9.715172392050214E-4	2.929972626173874E-4
130-134	0.0212088895712165	3.5639433113693905E-4	5.894213938033991E-4	8.378693650286692E-4	9.955053191853924E-4	3.084181711761972E-4
135-139	0.021498459965265258	3.632480682741879E-4	6.271169480582677E-4	8.85845524989411E-4	0.0010212068334500752	3.238390797350071E-4
140-144	0.021678370565118045	3.683883711271245E-4	6.425378566170776E-4	9.406754220874017E-4	0.0010297740048716363	3.3583311972519255E-4
145-149	0.021770896016470902	3.769555425486855E-4	6.45964725185702E-4	9.578097649305237E-4	0.0010451949134304462	3.5125402828400243E-4
150-154	0.021854854296402198	3.769555425486855E-4	6.733796737346975E-4	9.68090370636397E-4	0.001091457639106876	3.786689768329977E-4
155-159	0.02207246045050985	3.8380927968593434E-4	6.853737137248828E-4	9.989321877540169E-4	0.001117159153371559	3.9066301682318317E-4
160-164	0.02225237105036263	3.9066301682318317E-4	6.956543194307559E-4	0.001010926227744202	0.0011428606676362417	3.940898853918076E-4
165-167	0.02241743188641804	3.940898853918076E-4	7.053637803751917E-4	0.0010309162943945111	0.0011480009704891785	3.940898853918076E-4
>>END_MODULE
