##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293669
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7311948
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.91351661691248	41.0	40.0	41.0	38.0	41.0
2	40.03220031105254	41.0	40.0	41.0	38.0	41.0
3	40.085700007713406	41.0	40.0	41.0	38.0	41.0
4	40.110104037939	41.0	40.0	41.0	38.0	41.0
5	40.112554957994774	41.0	40.0	41.0	38.0	41.0
6	40.13781908733487	41.0	40.0	41.0	38.0	41.0
7	40.11788130878392	41.0	40.0	41.0	38.0	41.0
8	40.09997691449666	41.0	40.0	41.0	38.0	41.0
9	40.07924057993848	41.0	40.0	41.0	38.0	41.0
10-14	40.02419048931967	41.0	40.0	41.0	38.0	41.0
15-19	39.881328423013954	41.0	40.0	41.0	38.0	41.0
20-24	39.69241559157697	40.4	39.6	41.0	37.2	41.0
25-29	39.43712720604687	40.0	39.0	41.0	36.4	41.0
30-34	39.29214887742637	40.0	38.6	41.0	35.6	41.0
35-39	38.900545545455195	40.0	37.8	41.0	35.0	41.0
40-44	38.429840447443006	39.6	36.6	41.0	35.0	41.0
45-49	37.89880338317504	38.8	35.2	40.8	34.6	41.0
50-54	37.1617610792637	37.0	35.0	39.6	34.0	41.0
55-59	36.41845029532486	35.8	35.0	38.8	34.0	40.6
60-64	35.43497858573393	34.8	34.4	36.6	33.2	39.2
65-69	35.39771934920763	35.0	35.0	36.0	33.6	38.4
70-74	35.17890934125899	35.0	35.0	35.6	33.8	37.0
75-79	35.030387743457695	35.0	35.0	35.0	33.0	36.4
80-84	34.96679921684344	35.0	35.0	35.0	33.0	36.0
85-89	35.8054756406911	36.2	35.8	36.2	33.6	37.0
90-94	39.888422160070725	41.0	40.0	41.0	38.0	41.0
95-99	39.84650466685342	41.0	40.0	41.0	37.6	41.0
100-104	39.71680283645945	41.0	40.0	41.0	37.0	41.0
105-109	39.54856917658013	40.8	39.0	41.0	36.2	41.0
110-114	39.32840480633355	40.0	38.8	41.0	35.4	41.0
115-119	39.02740276246116	40.0	38.0	41.0	35.0	41.0
120-124	38.844906807517816	40.0	37.8	41.0	35.0	41.0
125-129	38.011315997694155	39.0	36.0	40.2	34.2	40.6
130-134	38.00541273035157	39.0	35.4	41.0	34.4	41.0
135-139	37.31622122639488	37.4	35.0	40.2	34.0	41.0
140-144	36.55298430844033	36.2	35.0	39.0	33.6	40.8
145-149	36.049698206674314	35.2	35.0	37.6	34.0	39.6
150-154	35.48255553143779	35.0	35.0	36.4	34.0	38.2
155-159	35.044243021509786	35.0	35.0	35.6	33.8	36.8
160-164	34.772020940042395	35.0	35.0	35.0	33.0	36.0
165-169	34.62355926179975	35.0	35.0	35.0	33.0	36.0
170-174	34.533840028708426	35.0	35.0	35.0	33.0	35.2
175-176	34.15018932882858	35.0	34.0	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	8.0
30	124.0
31	1654.0
32	11872.0
33	51503.0
34	163436.0
35	442602.0
36	1175521.0
37	2505895.0
38	2520981.0
39	436728.0
40	1623.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.905113657810478	27.979083002231413	27.9252259452611	21.19057739469701
2	20.931932229277344	27.34227595710473	29.367372415668164	22.358419397949767
3	21.451602226930497	26.590150805230017	29.6309273534221	22.32731961441739
4	22.28712512725747	27.248374851681113	27.99471495147394	22.469785069587473
5	22.218306257101393	27.521311694229773	28.02127422131558	22.23910782735326
6	22.12450088540017	27.566744183629314	27.820356490500203	22.48839844047031
7	22.3139989507584	27.75388993466584	27.505255781359494	22.426855333216263
8	22.350254679054064	27.896533181034656	27.63237648845424	22.120835651457043
9	22.131694590825866	27.728876080628584	27.689816721891347	22.449612606654203
10-14	22.361403554839285	27.721277558319617	27.561265479459102	22.356053407382
15-19	22.32978133870755	27.613746706076135	27.568393538903724	22.488078416312586
20-24	22.370604933186065	27.494541810198868	27.591643157199698	22.54321009941537
25-29	22.452763613745613	27.540852314595238	27.407910997178863	22.59847307448029
30-34	22.431513462623094	27.478045522205573	27.413712460756013	22.67672855441532
35-39	22.42510477372104	27.526423875005673	27.443236740742687	22.6052346105306
40-44	22.546155962815927	27.494202639296667	27.321890144732976	22.637751253154427
45-49	22.64367306769687	27.385249457463317	27.291608200714773	22.679469274125037
50-54	22.621564048322007	27.415928012617158	27.327035148499412	22.63547279056142
55-59	22.62833105487074	27.420534172288974	27.28680783834896	22.664326934491328
60-64	22.575811534764746	27.405539536112677	27.309190382644953	22.709458546477627
65-69	22.567821871818563	27.395304233564023	27.358162284523907	22.678711610093508
70-74	22.5596434766768	27.444240577203228	27.329524225281688	22.666591720838277
75-79	22.51325912055173	27.38843875804368	27.32609148752152	22.772210633883063
80-84	22.503652925321678	27.413523728560435	27.3697159771924	22.71310736892549
85-89	22.56302424470196	27.49632245743542	27.427840022932333	22.512813274930295
90-94	21.798706413087892	27.303920430122176	28.47376654294228	22.42360661384765
95-99	22.26454286044107	27.784520749917803	27.627105937531965	22.323830452109164
100-104	22.285641061353413	27.64370083928761	27.58345295735064	22.48720514200834
105-109	22.315655575179527	27.569856804280747	27.624682520310206	22.489805100229525
110-114	22.386769169055615	27.497209252417193	27.51510412241382	22.600917456113375
115-119	22.416511859795126	27.521700517569396	27.403783960952005	22.65800366168347
120-124	22.407801475660758	27.5278156645249	27.452435935075947	22.61194692473839
125-129	22.42322805082977	27.557979420389163	27.42003553805658	22.598756990724493
130-134	22.508165704241286	27.471280700031997	27.332362004526757	22.68819159119996
135-139	22.57711252393313	27.49767466595332	27.327945767439317	22.597267042674225
140-144	22.549021803901468	27.56649040189417	27.318754564326643	22.56573322987772
145-149	22.468989882064104	27.56681808543664	27.369971967719238	22.59422006478002
150-154	22.430421612313125	27.63123726513846	27.4757596621755	22.462581460372917
155-159	22.400967115481198	27.674387341559537	27.46690899332618	22.457736549633093
160-164	22.339467545802254	27.69728607156747	27.491460583163494	22.471785799466783
165-169	22.304543717789336	27.742558276918132	27.54322248723369	22.40967551805884
170-174	22.255064274351692	27.853472412224033	27.54683992466572	22.34462338875856
175-176	22.222618967527605	27.79272518214207	27.53066041723855	22.453995433091773
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	4.5
12	6.5
13	18.5
14	42.5
15	78.5
16	140.0
17	232.5
18	439.0
19	781.5
20	1416.0
21	2644.0
22	4482.5
23	7370.0
24	11729.5
25	17545.0
26	25051.0
27	34507.0
28	45869.0
29	59039.0
30	74015.0
31	90831.0
32	109013.5
33	128622.0
34	150161.0
35	173011.0
36	197486.0
37	222297.5
38	246826.5
39	270850.0
40	292924.5
41	312587.5
42	329101.5
43	341167.0
44	346677.0
45	345646.0
46	339392.0
47	326948.0
48	309047.0
49	287191.5
50	262552.5
51	237759.5
52	212410.5
53	188088.5
54	166559.0
55	147414.5
56	130953.5
57	117021.0
58	104969.5
59	93936.0
60	84549.5
61	76272.5
62	68012.5
63	60043.5
64	52323.0
65	44827.5
66	37508.0
67	30711.0
68	24655.5
69	19227.0
70	14512.0
71	10710.5
72	7656.0
73	5216.0
74	3545.0
75	2368.0
76	1524.5
77	924.0
78	514.0
79	278.0
80	155.0
81	86.0
82	47.0
83	18.0
84	9.5
85	5.5
86	1.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	741.0
92-93	1824.0
94-95	2901.0
96-97	3936.0
98-99	4793.0
100-101	5655.0
102-103	6467.0
104-105	7234.0
106-107	7959.0
108-109	8850.0
110-111	9077.0
112-113	9563.0
114-115	9930.0
116-117	10372.0
118-119	10439.0
120-121	10935.0
122-123	11462.0
124-125	12061.0
126-127	12422.0
128-129	13208.0
130-131	13984.0
132-133	43535.0
134-135	76637.0
136-137	76455.0
138-139	76602.0
140-141	78838.0
142-143	80071.0
144-145	70512.0
146-147	67823.0
148-149	68352.0
150-151	66540.0
152-153	60851.0
154-155	60688.0
156-157	62372.0
158-159	64609.0
160-161	66458.0
162-163	68406.0
164-165	69626.0
166-167	73406.0
168-169	76051.0
170-171	79388.0
172-173	83247.0
174-175	167520.0
176-177	5500148.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.34408496210288
#Duplication Level	Percentage of total
1	93.14048815175163
2	5.837399775362283
3	0.6089320233861379
4	0.18158719948894197
5	0.08525253940165982
6	0.054810305381815194
7	0.031236174860611393
8	0.012579436695330461
9	0.020052569298400224
>10	0.02622855583117282
>50	0.0014332685420233061
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.73524921129089E-5	0.0	0.0	0.0	0.0	0.0
2	2.73524921129089E-5	0.0	0.0	0.0	0.0	0.0
3	4.102873816936335E-5	0.0	1.367624605645445E-5	1.367624605645445E-5	1.367624605645445E-5	0.0
4	4.102873816936335E-5	2.73524921129089E-5	1.367624605645445E-5	1.367624605645445E-5	1.367624605645445E-5	0.0
5	4.102873816936335E-5	2.73524921129089E-5	1.367624605645445E-5	2.73524921129089E-5	1.367624605645445E-5	0.0
6	4.102873816936335E-5	2.73524921129089E-5	2.73524921129089E-5	4.102873816936335E-5	1.367624605645445E-5	0.0
7	5.47049842258178E-5	2.73524921129089E-5	2.73524921129089E-5	5.47049842258178E-5	1.367624605645445E-5	0.0
8	5.47049842258178E-5	5.47049842258178E-5	2.73524921129089E-5	5.47049842258178E-5	1.367624605645445E-5	0.0
9	6.838123028227225E-5	5.47049842258178E-5	2.73524921129089E-5	5.47049842258178E-5	1.367624605645445E-5	0.0
10-14	7.658697791614491E-5	6.564598107098136E-5	3.8293488958072464E-5	7.111647949356314E-5	1.367624605645445E-5	0.0
15-19	1.094099684516356E-4	8.20574763387267E-5	7.111647949356314E-5	8.479272555001759E-5	1.367624605645445E-5	0.0
20-24	1.1761571608550825E-4	9.573372239518115E-5	8.20574763387267E-5	1.1761571608550826E-4	1.367624605645445E-5	0.0
25-29	1.3129196214196271E-4	1.0120422081776293E-4	9.846897160647203E-5	1.5317395583228983E-4	4.376398738065424E-5	0.0
30-34	1.5864445425487161E-4	1.3129196214196271E-4	1.2308621450809004E-4	1.8873219557907138E-4	8.20574763387267E-5	0.0
35-39	1.914674447903623E-4	1.5043870662099896E-4	1.367624605645445E-4	2.461724290161801E-4	8.20574763387267E-5	1.094099684516356E-5
40-44	2.2429043532585296E-4	1.641149526774534E-4	1.5043870662099894E-4	3.1455365929845233E-4	9.846897160647203E-5	4.6499236591945135E-5
45-49	2.7078967191779816E-4	1.7232070031132608E-4	1.5317395583228983E-4	3.665233943129793E-4	1.367624605645445E-4	7.932222712743581E-5
50-54	2.817306687629617E-4	1.8873219557907138E-4	1.7505594952261697E-4	3.829348895807246E-4	1.367624605645445E-4	1.1214521766292648E-4
55-59	2.9267166560812525E-4	2.3249618295972564E-4	2.2155518611456206E-4	3.938758864258882E-4	1.613797034661625E-4	1.367624605645445E-4
60-64	3.0087741324199787E-4	2.4617242901618007E-4	2.6258392428392543E-4	4.1849312932750615E-4	1.8873219557907138E-4	1.641149526774534E-4
65-69	3.2002415772103407E-4	2.5711342586134364E-4	2.762601703403799E-4	4.513161198629968E-4	2.352314321710165E-4	2.0787894005810763E-4
70-74	6.291073185969047E-4	2.98142164030707E-4	3.0087741324199787E-4	4.5405136907428775E-4	2.5984867507263453E-4	2.4343717980488915E-4
75-79	0.0028364534321086527	3.364356529887794E-4	3.0087741324199787E-4	5.005506056662329E-4	3.0634791166457966E-4	2.6805442270650727E-4
80-84	0.003347945034620049	3.6652339431297924E-4	3.1181841008716144E-4	5.060211040888147E-4	4.97815356454942E-4	2.73524921129089E-4
85-89	0.003391709022000704	4.0481688327105163E-4	3.6105289589039746E-4	5.361088454130145E-4	7.111647949356313E-4	2.73524921129089E-4
90-94	0.0033971795204232854	4.1028738169363347E-4	3.856701387920155E-4	5.962843280614141E-4	7.111647949356313E-4	2.73524921129089E-4
95-99	0.003430002510958776	4.2943412617266967E-4	4.1849312932750615E-4	6.619303091323953E-4	7.111647949356313E-4	2.73524921129089E-4
100-104	0.003449149255437812	4.7046286434203306E-4	4.321693753839606E-4	7.056942965130497E-4	7.248410409920859E-4	2.73524921129089E-4
105-109	0.003471031249128139	4.868743596097783E-4	4.513161198629968E-4	7.576640315275765E-4	7.248410409920859E-4	2.73524921129089E-4
110-114	0.003501118990452339	5.032858548775237E-4	4.513161198629968E-4	8.205747633872669E-4	7.248410409920859E-4	2.73524921129089E-4
115-119	0.003512059987297503	5.060211040888147E-4	4.759333627646148E-4	8.561330031340485E-4	7.330467886259585E-4	2.73524921129089E-4
120-124	0.0035202657349313756	5.060211040888147E-4	4.923448580323601E-4	9.081027381485755E-4	7.549287823162855E-4	2.73524921129089E-4
125-129	0.0035394124794104116	5.060211040888147E-4	4.923448580323601E-4	9.190437349937391E-4	7.93222271274358E-4	2.73524921129089E-4
130-134	0.003547618227044284	5.251678485678509E-4	5.2243259935656E-4	9.546019747405206E-4	8.315157602324305E-4	2.73524921129089E-4
135-139	0.0035941174636362296	5.579908391033416E-4	5.470498422581781E-4	9.874249652760112E-4	8.342510094437215E-4	3.036126624532888E-4
140-144	0.00365702819549592	6.099605741178685E-4	5.880785804275413E-4	0.0010393947002905381	8.342510094437215E-4	3.2002415772103407E-4
145-149	0.0036843806876088285	6.591950599211044E-4	6.126958233291593E-4	0.0010694824416147381	8.342510094437215E-4	3.282299053549068E-4
150-154	0.0037062626812991556	7.029590473017587E-4	6.23636820174323E-4	0.0011077759305728105	8.397215078663032E-4	3.5011189904523394E-4
155-159	0.0037472914194685196	7.22105791780795E-4	6.291073185969047E-4	0.0011241874258405555	8.752797476130848E-4	3.5558239746781567E-4
160-164	0.003782849659215301	7.467230346824129E-4	6.291073185969047E-4	0.0011488046687421738	8.97161741303412E-4	3.5831764667910656E-4
165	0.0038156726497507914	7.658697791614492E-4	6.291073185969047E-4	0.0011624809147986281	9.163084857824481E-4	3.6925864352427013E-4
>>END_MODULE
