##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293675
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6008570
Total Bases	999.7 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.70176880688749	39.0	37.0	39.0	35.0	39.0
2	39.335339523380775	40.0	39.0	41.0	36.0	41.0
3	39.423389924724184	40.0	39.0	41.0	37.0	41.0
4	39.460518559324434	40.0	39.0	41.0	37.0	41.0
5	39.482399639182034	40.0	39.0	41.0	37.0	41.0
6	39.47267735917198	40.0	39.0	41.0	37.0	41.0
7	39.51117237545706	40.0	39.0	41.0	37.0	41.0
8	39.497069685465924	40.0	39.0	41.0	37.0	41.0
9	39.49258009809322	40.0	39.0	41.0	37.0	41.0
10-14	39.43126550909784	40.0	39.0	41.0	36.6	41.0
15-19	39.287222017884446	40.0	38.8	41.0	36.0	41.0
20-24	39.11590454966823	40.0	38.0	41.0	35.8	41.0
25-29	38.912344467984894	40.0	38.0	41.0	35.0	41.0
30-34	38.822751436697914	40.0	38.0	41.0	35.0	41.0
35-39	38.464671627358925	40.0	37.2	41.0	34.2	41.0
40-44	38.20181520727893	39.4	36.4	40.8	34.0	41.0
45-49	37.5989475033161	38.6	35.2	40.0	33.6	41.0
50-54	36.8950693093365	37.0	35.0	39.8	33.0	41.0
55-59	36.18408709559845	35.8	34.8	38.8	33.0	40.4
60-64	35.439249838147845	35.0	33.8	37.0	32.4	39.6
65-69	35.065358446352455	35.0	34.0	36.0	32.4	39.0
70-74	34.83485721228179	35.0	34.0	36.0	32.0	37.4
75-79	34.66933263655079	35.0	34.0	35.0	32.0	37.0
80-84	34.61475525790662	35.0	34.0	35.0	32.0	37.0
85-89	34.47907981433186	34.8	33.8	35.0	31.6	37.8
90-94	38.14680796484884	39.8	37.4	40.6	32.8	40.6
95-99	38.44375111960274	40.0	37.8	41.0	33.0	41.0
100-104	38.33964350669755	40.0	37.0	41.0	33.0	41.0
105-109	38.19177701088708	40.0	37.0	41.0	33.0	41.0
110-114	37.99966909582499	40.0	36.2	41.0	32.6	41.0
115-119	37.77757271754008	39.6	35.8	40.4	32.0	41.0
120-124	37.534435337636815	39.0	35.0	40.0	32.0	41.0
125-129	37.13657893694024	38.6	34.8	39.8	31.8	40.6
130-134	36.95306340827522	38.0	34.8	40.0	31.8	41.0
135-139	36.64716339462494	37.2	34.2	40.0	31.4	41.0
140-144	36.30337110637659	36.2	34.0	39.4	32.0	41.0
145-149	35.70453947344097	35.2	34.0	38.4	31.2	40.2
150-154	35.110076100492606	35.0	34.0	36.8	31.0	39.2
155-159	34.62691664538683	35.0	34.0	36.0	31.0	37.6
160-164	34.267763335259055	35.0	34.0	35.0	31.0	36.4
165-169	34.042889800292315	35.0	34.0	35.0	31.0	36.0
170-174	33.92728572995263	35.0	34.0	35.0	31.0	35.6
175-178	33.669764963204095	34.75	32.75	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	65.0
29	1158.0
30	7537.0
31	31938.0
32	109357.0
33	248088.0
34	416566.0
35	693816.0
36	1179755.0
37	1722606.0
38	1333883.0
39	262160.0
40	1641.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.759786438370526	28.16435524592374	28.325508398836995	21.75034991686874
2	22.26173615352738	28.704849906050857	28.47945517818716	20.553958762234608
3	20.60355458952796	28.029198295101832	29.575855819271474	21.791391296098737
4	21.06754186104181	27.435363156291764	29.959174978405844	21.53792000426058
5	21.785915783622393	27.975075600350834	28.654904577961144	21.58410403806563
6	21.73307459179139	28.050551129470076	28.55466442098536	21.661709857753173
7	21.681148759188957	28.25183030238476	28.395275414948983	21.671745523477302
8	21.867599112600836	28.430691495647054	28.159428948984534	21.54228044276758
9	21.923386096858323	28.377251159593715	28.103076106294843	21.596286637253122
10-14	21.871982851160926	28.374002466477048	28.082898260318178	21.67111642204385
15-19	21.78856200393771	28.362911641205812	28.166029521167268	21.682496833689214
20-24	21.749115014054926	28.253165062568964	28.294239727589094	21.703480195787016
25-29	21.724010871139058	28.28480986324533	28.19933528277111	21.7918439828445
30-34	21.703849668057458	28.32733911729413	28.245496016523063	21.723315198125345
35-39	21.70088723273591	28.335114677868447	28.206235427065007	21.757762662330638
40-44	21.757086960791003	28.3292297501735	28.219419928535412	21.694263360500084
45-49	21.92451448514372	28.230910183288206	28.10597197003613	21.738603361531943
50-54	21.871779807841133	28.32745228898057	28.127467933301936	21.67329996987636
55-59	21.826421261631303	28.27616221496962	28.137903028507615	21.759513494891465
60-64	21.74755723907685	28.34969385394528	28.18665339673167	21.7160955102462
65-69	21.731703217238042	28.322635835148795	28.185967709454996	21.759693238158164
70-74	21.650302817475705	28.38418791825676	28.197993865428877	21.767515398838658
75-79	21.645626163962472	28.372963949824996	28.182332901172824	21.7990769850397
80-84	21.61682396976319	28.415493203873798	28.157438458734774	21.810244367628236
85-89	21.58109833121691	28.41571954724668	28.134903978816922	21.868278142719483
90-94	21.227676125963075	28.300799831635427	28.843186179719194	21.628337862682304
95-99	21.416119859287495	28.50723588035432	28.32553117636457	21.751113083993616
100-104	21.538022617618868	28.510210332935564	28.16123242537316	21.790534624072407
105-109	21.428695930771536	28.517737057836158	28.28005778231393	21.773509229078382
110-114	21.43393319711828	28.394566376380492	28.299013664543793	21.872486761957436
115-119	21.4588066924775	28.45305364080935	28.203796060647324	21.88434360606583
120-124	21.414396979647364	28.47056789786257	28.25176174271858	21.863273379771485
125-129	21.41918738975239	28.466607530219505	28.249967596068487	21.864237483959617
130-134	21.54757218400829	28.47567282324511	28.172188977120378	21.80456601562623
135-139	21.664200319248703	28.441961602262133	28.094266443900747	21.799571634588418
140-144	21.626916899446556	28.475808996343282	28.199620555348943	21.697653548861222
145-149	21.601133467171053	28.518899788663987	28.18061859847533	21.699348145689626
150-154	21.500323928362555	28.59622865217151	28.26599479782224	21.63745262164369
155-159	21.458770576737447	28.612092515169163	28.288487188762744	21.640649719330643
160-164	21.403798261365427	28.726896718033906	28.317475422803685	21.551829597796978
165-169	21.326478506096542	28.737988182575975	28.387970509563658	21.547562801763824
170-174	21.28741487438277	28.807619149035844	28.421544718718895	21.483421257862492
175-178	21.231020631236774	28.916188567150087	28.41942096892214	21.433369832691003
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	2.0
13	6.0
14	16.5
15	37.5
16	100.0
17	234.0
18	493.5
19	1111.0
20	2278.5
21	4170.0
22	7241.5
23	11855.5
24	18020.5
25	25939.0
26	35843.5
27	47454.0
28	60238.5
29	74030.0
30	88971.0
31	104308.0
32	120414.0
33	136468.5
34	152662.0
35	169957.5
36	186531.0
37	203081.0
38	219627.0
39	234241.0
40	247947.0
41	260345.5
42	268996.0
43	273017.0
44	273398.5
45	271042.0
46	264098.0
47	253053.0
48	238941.5
49	222003.5
50	204161.0
51	185226.5
52	165363.0
53	146338.0
54	128121.0
55	111332.5
56	96147.5
57	82245.5
58	70137.5
59	59511.5
60	50294.0
61	42502.5
62	35951.5
63	30325.5
64	25430.5
65	21331.0
66	17612.5
67	14269.0
68	11465.0
69	9054.5
70	7024.5
71	5317.0
72	3986.5
73	2884.5
74	1984.5
75	1363.0
76	902.0
77	582.5
78	374.5
79	223.5
80	133.5
81	75.0
82	40.0
83	22.5
84	14.5
85	5.5
86	1.0
87	2.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1995.0
92-93	4199.0
94-95	5813.0
96-97	7943.0
98-99	9708.0
100-101	11531.0
102-103	12678.0
104-105	14726.0
106-107	15372.0
108-109	16929.0
110-111	18239.0
112-113	18997.0
114-115	19940.0
116-117	21111.0
118-119	21859.0
120-121	22362.0
122-123	23754.0
124-125	24111.0
126-127	25111.0
128-129	25825.0
130-131	26699.0
132-133	28494.0
134-135	117428.0
136-137	99344.0
138-139	90991.0
140-141	100847.0
142-143	100943.0
144-145	90577.0
146-147	88143.0
148-149	90600.0
150-151	90829.0
152-153	92405.0
154-155	94137.0
156-157	80438.0
158-159	86593.0
160-161	85558.0
162-163	87202.0
164-165	83906.0
166-167	88468.0
168-169	86664.0
170-171	105855.0
172-173	83972.0
174-175	95551.0
176-177	187683.0
178-179	3503040.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.45525541978478
#Duplication Level	Percentage of total
1	91.45445873104367
2	7.19993388331953
3	0.9560972189492611
4	0.20901583146865316
5	0.08782014961749562
6	0.037505385219849335
7	0.016808023524176614
8	0.008473870845804556
9	0.009612120530518145
>10	0.020274785481017486
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.6642895064882327E-5	0.0	0.0	0.0	0.0
2	3.328579012976465E-5	3.328579012976465E-5	0.0	0.0	0.0	0.0
3	3.328579012976465E-5	8.321447532441163E-5	0.0	1.6642895064882327E-5	0.0	0.0
4	3.328579012976465E-5	8.321447532441163E-5	1.6642895064882327E-5	4.992868519464698E-5	0.0	0.0
5	3.328579012976465E-5	8.321447532441163E-5	1.6642895064882327E-5	4.992868519464698E-5	0.0	0.0
6	3.328579012976465E-5	8.321447532441163E-5	3.328579012976465E-5	4.992868519464698E-5	0.0	0.0
7	3.328579012976465E-5	8.321447532441163E-5	3.328579012976465E-5	4.992868519464698E-5	0.0	0.0
8	4.992868519464698E-5	8.321447532441163E-5	3.328579012976465E-5	4.992868519464698E-5	0.0	0.0
9	4.992868519464698E-5	8.321447532441163E-5	3.328579012976465E-5	6.65715802595293E-5	0.0	0.0
10-14	7.65573172984587E-5	9.65287913763175E-5	4.660010618167051E-5	1.0318594940227042E-4	0.0	0.0
15-19	1.0984310742822335E-4	9.985737038929396E-5	7.322873828548224E-5	1.331431605190586E-4	0.0	0.0
20-24	1.1650026545417629E-4	1.0318594940227042E-4	9.320021236334103E-5	1.5644321360989386E-4	9.985737038929397E-6	0.0
25-29	1.1650026545417629E-4	1.264860024931057E-4	1.1650026545417629E-4	1.9638616176561146E-4	1.9971474077858793E-5	0.0
30-34	2.463148469602584E-4	1.4312889755798802E-4	1.3647173953203507E-4	2.596291630121643E-4	3.328579012976465E-5	0.0
35-39	4.593439037907522E-4	1.4978605558394094E-4	1.631003716358468E-4	3.2952932228467004E-4	3.328579012976465E-5	1.3314316051905862E-5
40-44	6.657158025952931E-4	1.5644321360989386E-4	1.8640042472668205E-4	4.0608663958312876E-4	3.9942948155717586E-5	1.6642895064882327E-5
45-49	8.72087701399834E-4	1.6642895064882327E-4	2.0970047781751732E-4	4.893011149075404E-4	7.322873828548224E-5	1.6642895064882327E-5
50-54	0.0010418452310616336	1.93057582752635E-4	2.3300053090835257E-4	5.691870112189756E-4	9.985737038929396E-5	1.6642895064882327E-5
55-59	0.0011883027076325982	2.196862148564467E-4	2.396576889343055E-4	5.991442223357638E-4	1.1650026545417629E-4	2.6628632103811723E-5
60-64	0.0013147887101257038	2.396576889343055E-4	2.7627205807704663E-4	6.357585914785049E-4	1.231574234801292E-4	4.660010618167051E-5
65-69	0.0014046603434760684	2.496434259732349E-4	3.195435852457407E-4	7.089873297639871E-4	1.6642895064882327E-4	6.65715802595293E-5
70-74	0.002010461723837785	2.596291630121643E-4	3.661436914274112E-4	7.322873828548224E-4	1.6642895064882327E-4	8.321447532441163E-5
75-79	0.012791729146868555	2.9291495314192896E-4	3.927723235312229E-4	7.389445408807753E-4	1.6975752966179972E-4	9.65287913763175E-5
80-84	0.017568240030489783	3.4284363833657594E-4	3.9942948155717584E-4	7.58916014958634E-4	2.8958637412895247E-4	9.985737038929396E-5
85-89	0.017651454505814197	3.728008494533641E-4	3.9942948155717584E-4	8.021875421273282E-4	5.092725889853991E-4	9.985737038929396E-5
90-94	0.01776795477126837	3.8944374451824643E-4	4.1607237662205816E-4	8.487876483089987E-4	5.29244063063258E-4	9.985737038929396E-5
95-99	0.01786781214165766	4.1607237662205816E-4	4.1607237662205816E-4	8.987163335036456E-4	5.325726420762344E-4	9.985737038929396E-5
100-104	0.01793105514290422	4.1607237662205816E-4	4.2605811366098757E-4	9.852593878410336E-4	5.359012210892109E-4	9.985737038929396E-5
105-109	0.018044226829345415	4.593439037907522E-4	4.327152716869405E-4	0.001065145284152469	5.658584322059991E-4	9.985737038929396E-5
110-114	0.01816405567381257	5.126011679983756E-4	4.4602958773884633E-4	0.00110175965329521	5.658584322059991E-4	9.985737038929396E-5
115-119	0.01829719883433163	5.525441161540933E-4	4.493581667518228E-4	0.0011150739693471159	5.658584322059991E-4	9.985737038929396E-5
120-124	0.018486927838071286	6.058013803617167E-4	4.6600106181670514E-4	0.0011317168644119982	5.758441692449285E-4	9.985737038929396E-5
125-129	0.018826442897394886	6.490729075304107E-4	4.992868519464698E-4	0.0012182599187493862	5.958156433227873E-4	9.985737038929396E-5
130-134	0.019235858115990993	6.657158025952931E-4	4.992868519464698E-4	0.0012748457619699862	6.157871174006461E-4	9.985737038929396E-5
135-139	0.01956871601728864	6.790301186471989E-4	4.992868519464698E-4	0.001294817236047845	6.157871174006461E-4	9.985737038929396E-5
140-144	0.019921545392664147	6.923444346991047E-4	4.992868519464698E-4	0.0013514030792684448	6.157871174006461E-4	9.985737038929396E-5
145-149	0.02020780318778012	6.990015927250577E-4	4.992868519464698E-4	0.0013913460274241625	6.424157495044578E-4	9.985737038929396E-5
150-154	0.020467432350792285	7.089873297639871E-4	4.992868519464698E-4	0.001424631817553927	6.590586445693401E-4	9.985737038929396E-5
155-159	0.020860204674323507	7.22301645815893E-4	5.092725889853991E-4	0.0015011891348523858	6.72372960621246E-4	9.985737038929396E-5
160-164	0.021159776785491392	7.788874890364929E-4	5.159297470113521E-4	0.001574417873137868	6.823586976601754E-4	9.985737038929396E-5
165-167	0.021341739104867436	7.988589631143517E-4	5.159297470113521E-4	0.0016088131896052915	6.823586976601754E-4	9.985737038929396E-5
>>END_MODULE
