##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293677
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5177070
Total Bases	856.4 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.642345573847756	39.0	37.0	39.0	35.0	39.0
2	39.24696440264474	40.0	38.0	41.0	36.0	41.0
3	39.34655915411613	40.0	39.0	41.0	36.0	41.0
4	39.39336168914077	40.0	39.0	41.0	36.0	41.0
5	39.42083591684099	40.0	39.0	41.0	37.0	41.0
6	39.419903536170075	40.0	39.0	41.0	37.0	41.0
7	39.46721099000013	40.0	39.0	41.0	37.0	41.0
8	39.445281404346474	40.0	39.0	41.0	37.0	41.0
9	39.43455429422434	40.0	39.0	41.0	37.0	41.0
10-14	39.360757996318384	40.0	39.0	41.0	36.2	41.0
15-19	39.23610208090677	40.0	38.6	41.0	36.0	41.0
20-24	39.06417487111436	40.0	38.0	41.0	35.6	41.0
25-29	38.85850819092653	40.0	38.0	41.0	35.0	41.0
30-34	38.761854098940134	40.0	38.0	41.0	35.0	41.0
35-39	38.41064099963879	40.0	37.2	40.8	34.2	41.0
40-44	38.14357870378418	39.4	36.4	40.2	34.0	41.0
45-49	37.53724635749565	38.6	35.2	40.0	33.4	41.0
50-54	36.82954091793234	36.8	35.0	39.8	33.0	41.0
55-59	36.11631583115546	35.8	34.4	38.8	33.0	40.2
60-64	35.367506330801014	35.0	33.8	37.0	32.0	39.4
65-69	34.99190426244961	35.0	34.0	36.0	32.0	38.8
70-74	34.7737042767434	35.0	34.0	35.8	32.0	37.4
75-79	34.60947389160278	35.0	34.0	35.0	32.0	37.0
80-84	34.544430575595854	35.0	34.0	35.0	32.0	37.0
85-89	34.397203089778586	34.8	33.8	35.0	31.6	37.4
90-94	37.785408217965866	39.8	36.4	40.6	32.2	40.6
95-99	38.145624279968914	40.0	37.0	41.0	32.6	41.0
100-104	38.04854183190811	40.0	36.4	41.0	32.0	41.0
105-109	37.90296335757301	40.0	36.0	41.0	32.0	41.0
110-114	37.716606855900984	39.8	35.6	40.4	32.0	41.0
115-119	37.48660221915494	39.0	35.0	40.0	32.0	41.0
120-124	37.26223709983667	38.8	35.0	40.0	31.6	41.0
125-129	36.878701824949275	38.2	34.6	39.8	31.0	40.6
130-134	36.69975868861175	37.8	34.0	40.0	31.0	41.0
135-139	36.40246777006705	37.0	34.0	40.0	31.0	41.0
140-144	36.08854160092449	36.0	34.0	39.4	31.0	41.0
145-149	35.51514177800291	35.0	34.0	38.2	31.0	40.0
150-154	34.944426514929546	35.0	34.0	36.6	31.0	39.2
155-159	34.48035734585779	35.0	33.4	35.8	31.0	37.6
160-164	34.12435488696043	35.0	33.0	35.0	30.0	36.4
165-169	33.9066800015978	35.0	33.0	35.0	30.0	36.0
170-174	33.80162535142242	35.0	33.0	35.0	30.0	35.6
175-178	33.56208749052606	34.75	32.75	35.0	29.75	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	85.0
29	1213.0
30	7722.0
31	33012.0
32	110767.0
33	252564.0
34	409783.0
35	643142.0
36	1037565.0
37	1432792.0
38	1050961.0
39	196200.0
40	1263.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.665923002779564	28.314896263716737	28.366682312582213	21.652498420921486
2	22.176327536618203	28.790493464449966	28.5429596277431	20.490219371188722
3	20.51567778685627	28.107076010175643	29.648604326385385	21.7286418765827
4	20.992491892132037	27.49267442781342	30.01587770688826	21.49895597316629
5	21.708804400944935	28.036302387257656	28.744965781803224	21.509927429994185
6	21.670095246925385	28.090502929263078	28.642707168340394	21.596694655471143
7	21.59059081681337	28.35184766673041	28.470833888666757	21.586727627789465
8	21.800110873524986	28.499614646894866	28.24331137110373	21.456963108476415
9	21.847782626080004	28.466970699642847	28.15248779715167	21.53275887712548
10-14	21.790456764154243	28.459468386558417	28.170246877094574	21.579827972192767
15-19	21.709306615518045	28.434724660860294	28.271207459045367	21.584761264576294
20-24	21.672892195778694	28.32643947252017	28.37135290811212	21.62931542358902
25-29	21.653189931756767	28.377452883580865	28.245536567981507	21.723820616680865
30-34	21.62158518235218	28.41265039877769	28.315637996009325	21.650126422860808
35-39	21.635511978783367	28.427994985580646	28.260143285680893	21.67634974995509
40-44	21.673993204650507	28.434620354756646	28.28283952119635	21.608546919396492
45-49	21.842405066958722	28.31515509738134	28.18677746292787	21.655662372732067
50-54	21.790425858642053	28.407207165442998	28.197992300664275	21.604374675250675
55-59	21.747764662251043	28.37299476344728	28.208457679730042	21.67078289457164
60-64	21.65597915423203	28.445622717096736	28.251107286553978	21.647290842117258
65-69	21.646869754513652	28.433372544701925	28.246085140822895	21.673672559961524
70-74	21.56921192875507	28.482597299244556	28.273123600801224	21.675067171199153
75-79	21.55319514706195	28.43638196895155	28.281819639294042	21.72860324469246
80-84	21.528887961723523	28.506189794613558	28.240591686030903	21.72433055763202
85-89	21.49782019559326	28.52372480959307	28.198714717011747	21.779740277801924
90-94	21.088789494377437	28.399796075019996	28.90618813219536	21.605226298407207
95-99	21.30313910104988	28.59602305863379	28.399368445758306	21.701469394558025
100-104	21.39101095469065	28.607354458320444	28.264604917989654	21.73702966899925
105-109	21.30284686585347	28.616424589380568	28.375503887650805	21.70522465711516
110-114	21.322860662085528	28.489249903032892	28.400265536139592	21.787623898741987
115-119	21.342204068002488	28.55459107280159	28.305331681482027	21.7978731777139
120-124	21.306252166901793	28.567237994282497	28.33861841058118	21.787891428234527
125-129	21.31472427713238	28.56398088505853	28.35816041786166	21.763134419947423
130-134	21.4482048572183	28.56939829842755	28.25871825951601	21.723678584838144
135-139	21.570575542148653	28.544668999985507	28.17165090708187	21.713104550783967
140-144	21.533917539462934	28.576209592574088	28.26641161555312	21.62346125240986
145-149	21.49362475507267	28.621345505331764	28.277241624293364	21.607788115302203
150-154	21.418566612357527	28.68197540332455	28.342712292472854	21.556745691845066
155-159	21.385795380042087	28.69243711414507	28.388009752055947	21.533757753756895
160-164	21.31435713184395	28.81272308465453	28.418546691093322	21.4543730924082
165-169	21.236973508214486	28.8505458451617	28.446300142410745	21.466180504213064
170-174	21.16543783692885	28.9141012112616	28.510804717132327	21.40965623467722
175-178	21.143981321145496	28.984802506344042	28.502986206839058	21.368229965671397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.5
13	5.5
14	11.5
15	24.5
16	74.0
17	213.5
18	465.5
19	988.5
20	1991.0
21	3752.0
22	6489.5
23	10392.5
24	15941.5
25	23163.5
26	31855.5
27	42015.5
28	53515.0
29	65856.5
30	78542.0
31	91945.0
32	105955.0
33	120100.5
34	134418.5
35	148547.0
36	163176.5
37	177884.5
38	191472.0
39	204359.0
40	216247.5
41	226312.5
42	233022.5
43	235825.0
44	235818.5
45	233405.5
46	227305.0
47	217039.5
48	204523.5
49	189860.0
50	173589.5
51	156747.0
52	139614.0
53	123494.5
54	107961.5
55	93451.0
56	80366.0
57	68498.5
58	58386.0
59	49525.0
60	41952.0
61	35464.0
62	29801.5
63	25111.0
64	21073.0
65	17524.5
66	14381.0
67	11655.0
68	9425.5
69	7517.0
70	5767.0
71	4280.0
72	3183.0
73	2384.0
74	1648.0
75	1068.0
76	732.0
77	477.5
78	280.0
79	167.5
80	101.0
81	55.5
82	33.5
83	13.5
84	5.5
85	2.0
86	1.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1703.0
92-93	3799.0
94-95	5457.0
96-97	7241.0
98-99	9106.0
100-101	10656.0
102-103	11620.0
104-105	13134.0
106-107	14382.0
108-109	15773.0
110-111	16850.0
112-113	17567.0
114-115	18609.0
116-117	19544.0
118-119	20625.0
120-121	21083.0
122-123	22452.0
124-125	22162.0
126-127	22609.0
128-129	23889.0
130-131	24819.0
132-133	26095.0
134-135	102502.0
136-137	95950.0
138-139	86977.0
140-141	89126.0
142-143	94148.0
144-145	94889.0
146-147	79454.0
148-149	87538.0
150-151	94251.0
152-153	90715.0
154-155	83423.0
156-157	76636.0
158-159	74682.0
160-161	73701.0
162-163	83959.0
164-165	76263.0
166-167	82875.0
168-169	80320.0
170-171	87803.0
172-173	90257.0
174-175	90847.0
176-177	167352.0
178-179	2844227.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.57117510761782
#Duplication Level	Percentage of total
1	91.5968144376625
2	7.262061157209986
3	0.8334265910453701
4	0.17182340114514405
5	0.05714340623058876
6	0.0346210930828137
7	0.016949466320588392
8	0.01069623826129394
9	0.007559793766920607
>10	0.008904415274791548
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.9315945119536727E-5	0.0	0.0	1.9315945119536727E-5	0.0	0.0
2	1.9315945119536727E-5	1.9315945119536727E-5	0.0	3.8631890239073455E-5	0.0	0.0
3	1.9315945119536727E-5	5.794783535861018E-5	0.0	3.8631890239073455E-5	0.0	0.0
4	1.9315945119536727E-5	7.726378047814691E-5	0.0	3.8631890239073455E-5	0.0	0.0
5	1.9315945119536727E-5	7.726378047814691E-5	0.0	5.794783535861018E-5	0.0	0.0
6	1.9315945119536727E-5	7.726378047814691E-5	0.0	5.794783535861018E-5	0.0	0.0
7	1.9315945119536727E-5	7.726378047814691E-5	0.0	5.794783535861018E-5	0.0	0.0
8	3.8631890239073455E-5	7.726378047814691E-5	0.0	5.794783535861018E-5	0.0	0.0
9	3.8631890239073455E-5	7.726378047814691E-5	0.0	5.794783535861018E-5	0.0	0.0
10-14	4.24950792629808E-5	9.271653657377628E-5	3.090551219125876E-5	9.271653657377628E-5	7.72637804781469E-6	0.0
15-19	8.49901585259616E-5	1.3907480486066443E-4	5.7947835358610186E-5	1.4293799388457177E-4	1.9315945119536727E-5	0.0
20-24	1.429379938845718E-4	1.7384350607583054E-4	7.726378047814691E-5	1.9315945119536727E-4	1.9315945119536727E-5	0.0
25-29	2.163385853388113E-4	1.7384350607583054E-4	7.726378047814691E-5	2.781496097213289E-4	3.476870121516611E-5	0.0
30-34	3.785925243429199E-4	1.7384350607583054E-4	7.726378047814691E-5	3.708661462951051E-4	6.953740243033222E-5	3.863189023907345E-6
35-39	6.335629999208046E-4	1.7770669509973788E-4	7.726378047814691E-5	3.940452804385492E-4	1.0430610364549833E-4	1.9315945119536727E-5
40-44	8.96259853546504E-4	2.202017743627187E-4	7.726378047814691E-5	4.674458718927887E-4	1.3134842681284976E-4	1.9315945119536727E-5
45-49	0.0011241880059570373	2.665600426496069E-4	9.271653657377628E-5	5.640255974904724E-4	1.622539390041085E-4	2.3179134143444074E-5
50-54	0.00130962107910459	3.090551219125876E-4	9.657972559768363E-5	6.181102438251752E-4	2.433809085061628E-4	4.635826828688814E-5
55-59	0.0014564222620130694	3.476870121516611E-4	9.657972559768363E-5	6.683317011359708E-4	3.399606341038464E-4	6.567421340642488E-5
60-64	0.0016379921461367145	3.5155020117556845E-4	1.197588597411277E-4	6.876476462555075E-4	3.670029572711978E-4	7.726378047814691E-5
65-69	0.001722982304662676	3.747293353190125E-4	1.5452756095629382E-4	7.49458670638025E-4	3.747293353190125E-4	7.726378047814691E-5
70-74	0.0023140502253205	3.8631890239073453E-4	1.6611712802801586E-4	8.460383962357087E-4	4.210876036059006E-4	7.726378047814691E-5
75-79	0.01402723934580757	3.8631890239073453E-4	1.7770669509973788E-4	8.730807194030601E-4	4.7517224994060353E-4	7.726378047814691E-5
80-84	0.019246407717106395	4.210876036059006E-4	1.9315945119536727E-4	9.078494206182262E-4	6.606053230881561E-4	7.726378047814691E-5
85-89	0.0193545770097758	4.674458718927888E-4	2.1247539631490396E-4	9.194389876899482E-4	0.0010469242254788906	7.726378047814691E-5
90-94	0.01947047268049302	4.944881950601403E-4	2.1247539631490396E-4	0.0010198819023115391	0.0010623769815745199	7.726378047814691E-5
95-99	0.01955932602804289	5.138041401796769E-4	2.24064963386626E-4	0.0010585137925506129	0.0010623769815745199	7.726378047814691E-5
100-104	0.0196713585097362	5.369832743231211E-4	2.3565453045834805E-4	0.0011512303291243888	0.0010623769815745199	7.726378047814691E-5
105-109	0.019756348668262164	5.562992194426577E-4	2.5110728655397747E-4	0.0011898622193634624	0.0010623769815745199	7.726378047814691E-5
110-114	0.019872244338979383	5.60162408466565E-4	2.5110728655397747E-4	0.0012632628108177018	0.0010623769815745199	7.726378047814691E-5
115-119	0.019957234497505343	5.60162408466565E-4	2.7042323167351414E-4	0.0013134842681284974	0.0010894193047418714	7.726378047814691E-5
120-124	0.02022765772917886	5.833415426100091E-4	3.167814999604023E-4	0.0013521161583675708	0.0011010088718135934	7.726378047814691E-5
125-129	0.02060625025352178	5.987942987056385E-4	3.476870121516611E-4	0.0013946112376305515	0.0011319143840048521	7.726378047814691E-5
130-134	0.021073696125414567	5.987942987056385E-4	3.631397682472905E-4	0.001421653560797903	0.0011589567071722037	7.726378047814691E-5
135-139	0.021394340814398876	6.103838657773605E-4	3.670029572711978E-4	0.0015182332863955866	0.0011589567071722037	7.726378047814691E-5
140-144	0.02173816463752663	6.335629999208046E-4	3.670029572711978E-4	0.001553001987610753	0.0011859990303395552	7.726378047814691E-5
145-149	0.022136073106989087	6.37426188944712E-4	3.670029572711978E-4	0.0015645915546824748	0.001197588597411277	7.726378047814691E-5
150-154	0.022464444174021214	6.451525669925266E-4	3.9404528043854927E-4	0.0015761811217541969	0.0012478100547220724	7.726378047814691E-5
155-159	0.022854626265435853	6.606053230881561E-4	4.2881398165371535E-4	0.0016070866339454558	0.001298031512032868	7.726378047814691E-5
160-164	0.023225492411730962	6.915108352794149E-4	4.5971949384497406E-4	0.001622539390041085	0.0013134842681284974	7.726378047814691E-5
165-167	0.02344311872674441	6.953740243033222E-4	4.764599796152393E-4	0.0016225393900410848	0.0013134842681284977	7.726378047814691E-5
>>END_MODULE
