##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293678
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4361080
Total Bases	712.6 Mbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.70122653104277	39.0	37.0	39.0	35.0	39.0
2	39.33609862694562	40.0	39.0	41.0	36.0	41.0
3	39.437371476790155	40.0	39.0	41.0	37.0	41.0
4	39.48835724178414	40.0	39.0	41.0	37.0	41.0
5	39.508487347170885	40.0	39.0	41.0	37.0	41.0
6	39.49402349876636	40.0	39.0	41.0	37.0	41.0
7	39.526660139231566	40.0	39.0	41.0	37.0	41.0
8	39.506611894301415	40.0	39.0	41.0	37.0	41.0
9	39.49366051528521	40.0	39.0	41.0	37.0	41.0
10-14	39.41600553074009	40.0	39.0	41.0	36.6	41.0
15-19	39.242856356682296	40.0	38.6	41.0	36.0	41.0
20-24	39.031399011254095	40.0	38.0	41.0	35.4	41.0
25-29	38.74844148697112	40.0	38.0	41.0	35.0	41.0
30-34	38.48068038192375	40.0	37.2	40.8	34.4	41.0
35-39	37.863730039348056	39.0	35.6	40.0	33.6	41.0
40-44	37.34087093105377	38.0	35.0	40.0	33.0	41.0
45-49	36.48495890926101	36.4	35.0	39.4	33.0	40.6
50-54	35.672058435066546	35.0	34.0	37.6	33.0	39.8
55-59	35.03516812349235	35.0	34.0	36.4	32.0	39.0
60-64	34.525115705284016	34.8	33.8	35.4	31.8	37.2
65-69	34.42074995184679	35.0	33.4	35.0	31.8	37.0
70-74	34.42737844754051	35.0	33.2	35.0	32.0	36.4
75-79	34.42808607042292	35.0	33.0	35.0	32.0	37.0
80-84	34.434647426784196	35.0	33.0	35.0	31.8	37.8
85-89	34.31090408797821	34.8	32.8	35.0	30.6	38.2
90-94	37.356897600928924	39.6	35.6	40.2	31.4	40.2
95-99	37.86263661001849	40.0	36.0	41.0	32.0	41.0
100-104	37.71218591312987	40.0	35.6	41.0	32.0	41.0
105-109	37.525631733625325	39.4	35.0	40.4	31.0	41.0
110-114	37.28867742256432	39.0	35.0	40.0	31.0	41.0
115-119	37.00591266150058	38.4	34.4	40.0	31.0	41.0
120-124	36.6364731250048	38.0	34.0	40.0	30.2	41.0
125-129	36.19109973482502	37.0	33.8	39.8	30.0	40.8
130-134	35.69019730645264	35.8	33.0	39.0	30.0	40.4
135-139	35.24901392923808	35.0	33.0	38.6	30.0	40.0
140-144	34.91034693604363	35.0	33.0	37.2	30.0	39.6
145-149	34.38028061159772	35.0	33.0	35.8	30.0	38.4
150-154	33.96410803060466	35.0	33.0	35.0	30.0	36.6
155-159	33.71227916235737	35.0	33.0	35.0	30.0	36.0
160-164	33.57994861835573	35.0	33.0	35.0	30.0	35.0
165-169	33.50771237248314	35.0	33.0	35.0	30.0	35.0
170-174	33.47724979672669	35.0	33.0	35.0	30.0	35.0
175-179	33.29308036681483	34.6	32.6	35.0	29.6	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	2.0
28	141.0
29	1508.0
30	9059.0
31	39933.0
32	135024.0
33	268874.0
34	412884.0
35	696681.0
36	1140362.0
37	1223794.0
38	419403.0
39	13378.0
40	37.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.73396498115146	25.334252066002	25.60432278243004	24.3274601704165
2	25.166380804754784	25.785195410311207	25.550872719601564	23.497551065332438
3	22.730814385427465	24.746622396287158	27.34467150338907	25.17789171489631
4	23.761980977189136	23.813780072825995	27.4659258715731	24.95831307841177
5	24.86306144349565	24.55978794243628	25.676185715464978	24.9009648986031
6	24.535275665660798	24.821466242306954	25.69487374687004	24.948384345162207
7	24.751024975464794	24.77345061315087	25.225150650756234	25.250373760628104
8	25.083075751877974	24.9112605134508	24.851412952754824	25.154250781916403
9	24.87143092995313	25.071885863134817	25.00392104708008	25.05276215983197
10-14	25.077146028048098	25.06771258495602	24.727434488704635	25.127706898291247
15-19	24.93716694029919	25.069638713346237	24.965866253313397	25.027328093041174
20-24	24.880754308565766	24.906775385913583	25.08695093875829	25.12551936676236
25-29	24.96077118511928	24.874058719399784	24.885138543663494	25.280031551817437
30-34	24.875553761912187	24.973575352894237	24.973011272437105	25.177859612756475
35-39	24.890096031258313	24.984636833078046	24.953447311216486	25.171819824447155
40-44	25.0049804176947	24.942959083529768	24.879759142230824	25.172301356544708
45-49	25.16059324754419	24.897351114861458	24.83410072734277	25.10795491025159
50-54	25.10475386830785	24.998041769469946	24.84294715987783	25.05425720234437
55-59	25.039609454538788	24.97679473891788	24.83744852192576	25.14614728461757
60-64	24.948544855861392	25.04910251589056	24.906050794757263	25.096301833490784
65-69	24.90902712172214	25.10487769084722	24.90875196052354	25.077343226907097
70-74	24.87979124437066	25.081305548166966	24.901024516862797	25.137878690599575
75-79	24.78442495895512	25.144776064644535	24.95947792748585	25.111321048914487
80-84	24.774074311867704	25.193328258137893	24.916722463243048	25.115874966751356
85-89	24.76591119630917	25.16321645097086	24.861162831225293	25.209709521494677
90-94	24.135811654492873	25.332969746140982	25.909519739586408	24.621698859779737
95-99	24.444227727377765	25.17263792498246	25.196896634105258	25.186237713534513
100-104	24.604870650800546	25.35411183724162	24.857644220853075	25.183373291104754
105-109	24.553451672922787	25.34803356627911	24.962644232437377	25.13587052836072
110-114	24.53769275006767	25.181973054689117	25.05029900618846	25.230035189054757
115-119	24.569396892785043	25.18518162247045	24.910673570234252	25.334747914510253
120-124	24.57941419742856	25.222616823547867	24.919834212134305	25.27813476688927
125-129	24.570596554167825	25.249731656474893	24.934797316995194	25.244874472362085
130-134	24.786624286890714	25.194433990269545	24.777139762588792	25.24180196025095
135-139	24.990700787315127	25.155981967982406	24.684004974356856	25.169312270345607
140-144	24.9272866958295	25.25034128135758	24.759287016514385	25.063085006298536
145-149	24.89140232859464	25.236618393102006	24.790037224636553	25.0819420536668
150-154	24.819047681530478	25.322537280233888	24.855875921005634	25.00253911723
155-159	24.77078323882925	25.37540197651542	24.883094586706157	24.970720197949174
160-164	24.722558182019046	25.43297415520452	24.887773382019624	24.956694280756807
165-169	24.638580555548	25.513226842871706	24.978984191214906	24.86920841036539
170-174	24.57249723677962	25.631741522271767	24.98333662538296	24.81242461556565
175-179	24.546141299523626	25.626636609758265	24.975460100008874	24.851761990709235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.5
18	5.5
19	16.5
20	37.5
21	69.5
22	143.5
23	269.5
24	435.0
25	715.5
26	1126.0
27	1719.0
28	2568.0
29	3672.5
30	5232.5
31	7228.0
32	9795.5
33	13597.0
34	18684.0
35	25230.0
36	33777.5
37	44976.0
38	59015.5
39	76095.0
40	96417.5
41	118608.0
42	140982.0
43	163509.5
44	184669.0
45	202101.5
46	214968.0
47	223020.0
48	226690.5
49	226366.5
50	221711.5
51	213572.5
52	203309.5
53	191389.5
54	178032.5
55	163398.5
56	148974.0
57	134508.5
58	120229.0
59	106775.5
60	94123.5
61	82657.5
62	72190.5
63	62637.0
64	53859.0
65	45727.0
66	38302.5
67	31620.0
68	25593.5
69	20159.0
70	15463.5
71	11589.5
72	8345.0
73	5843.5
74	4004.5
75	2602.5
76	1653.5
77	1054.0
78	604.0
79	326.5
80	171.0
81	90.5
82	46.0
83	19.5
84	10.0
85	4.5
86	2.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1751.0
92-93	3935.0
94-95	5713.0
96-97	7604.0
98-99	9160.0
100-101	10830.0
102-103	12009.0
104-105	13808.0
106-107	15243.0
108-109	16546.0
110-111	17274.0
112-113	18092.0
114-115	18975.0
116-117	20160.0
118-119	21509.0
120-121	21791.0
122-123	22667.0
124-125	23172.0
126-127	23976.0
128-129	25182.0
130-131	25722.0
132-133	27793.0
134-135	104544.0
136-137	102714.0
138-139	87709.0
140-141	89440.0
142-143	93195.0
144-145	86916.0
146-147	88180.0
148-149	91498.0
150-151	85143.0
152-153	78360.0
154-155	81505.0
156-157	78871.0
158-159	75745.0
160-161	82293.0
162-163	86967.0
164-165	73145.0
166-167	76345.0
168-169	78079.0
170-171	78771.0
172-173	76784.0
174-175	80533.0
176-177	87048.0
178-179	2134383.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.34869142662305
#Duplication Level	Percentage of total
1	91.41435111327236
2	6.9952878068497055
3	0.8861111700948572
4	0.2977573319023659
5	0.16628315057612267
6	0.09626179195264582
7	0.05884600174165624
8	0.03679215342415001
9	0.017510502111679004
>10	0.030798978074468412
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	4.5860199767030186E-5	0.0	2.2930099883515093E-5	0.0	0.0	0.0
3	4.5860199767030186E-5	6.879029965054527E-5	2.2930099883515093E-5	0.0	0.0	0.0
4	4.5860199767030186E-5	9.172039953406037E-5	2.2930099883515093E-5	0.0	0.0	0.0
5	4.5860199767030186E-5	9.172039953406037E-5	2.2930099883515093E-5	0.0	0.0	0.0
6	4.5860199767030186E-5	9.172039953406037E-5	2.2930099883515093E-5	0.0	0.0	0.0
7	4.5860199767030186E-5	9.172039953406037E-5	2.2930099883515093E-5	0.0	0.0	0.0
8	4.5860199767030186E-5	9.172039953406037E-5	2.2930099883515093E-5	0.0	0.0	0.0
9	4.5860199767030186E-5	9.172039953406037E-5	2.2930099883515093E-5	0.0	0.0	0.0
10-14	4.5860199767030186E-5	9.172039953406037E-5	2.2930099883515093E-5	9.172039953406038E-6	4.586019976703019E-6	0.0
15-19	4.5860199767030186E-5	9.172039953406037E-5	3.668815981362415E-5	3.668815981362415E-5	2.2930099883515093E-5	0.0
20-24	4.5860199767030186E-5	9.172039953406037E-5	6.420427967384226E-5	5.9618259697139245E-5	2.7516119860218114E-5	0.0
25-29	4.5860199767030186E-5	9.172039953406037E-5	6.879029965054527E-5	9.172039953406037E-5	5.503223972043622E-5	0.0
30-34	5.961825969713924E-5	9.172039953406037E-5	7.796233960395132E-5	9.172039953406037E-5	8.254835958065434E-5	1.3758059930109057E-5
35-39	7.33763196272483E-5	9.172039953406037E-5	1.0547845946416942E-4	9.172039953406037E-5	1.2382253937098152E-4	5.0446219743733204E-5
40-44	1.0089243948746641E-4	9.172039953406037E-5	1.6051069918460566E-4	1.0547845946416942E-4	1.971988589982298E-4	8.713437955735736E-5
45-49	1.5592467920790264E-4	9.172039953406037E-5	2.2930099883515092E-4	1.284085593476845E-4	2.751611986021811E-4	1.284085593476845E-4
50-54	2.1554293890504187E-4	1.0089243948746641E-4	2.797472185788841E-4	1.3758059930109055E-4	3.393654782760234E-4	1.3758059930109055E-4
55-59	2.2930099883515092E-4	1.3758059930109055E-4	3.118493584158053E-4	1.5133865923119962E-4	4.264998578333807E-4	1.696827391380117E-4
60-64	2.6598915864877506E-4	1.3758059930109055E-4	3.210213983692113E-4	1.8344079906812075E-4	5.182202573674411E-4	1.8802681904482376E-4
65-69	3.0726333843910227E-4	1.3758059930109055E-4	3.393654782760234E-4	1.8344079906812075E-4	6.282847368083135E-4	2.0637089895163583E-4
70-74	4.310858778100838E-4	1.696827391380117E-4	3.714676181129445E-4	1.8344079906812075E-4	6.97075036458859E-4	2.2012895888174488E-4
75-79	0.0022150476487475578	1.8344079906812075E-4	3.989837379731626E-4	2.0178487897493281E-4	7.200051363423738E-4	2.52231098718666E-4
80-84	0.0031001495042512405	2.1095691892833885E-4	4.264998578333807E-4	2.4305905876525997E-4	7.842094160162162E-4	2.52231098718666E-4
85-89	0.003201041943738707	2.2930099883515092E-4	4.448439377401928E-4	2.52231098718666E-4	8.667577755968704E-4	2.52231098718666E-4
90-94	0.0032514881634824406	2.52231098718666E-4	4.5860199767030184E-4	2.52231098718666E-4	8.805158355269796E-4	2.52231098718666E-4
95-99	0.003288176323296064	2.52231098718666E-4	4.5860199767030184E-4	2.6140313867207204E-4	8.942738954570886E-4	2.52231098718666E-4
100-104	0.0033065204032028763	2.52231098718666E-4	4.723600576004109E-4	3.164353783925083E-4	8.942738954570886E-4	2.52231098718666E-4
105-109	0.0033432085630165005	2.52231098718666E-4	5.04462197437332E-4	3.210213983692113E-4	8.942738954570886E-4	2.52231098718666E-4
110-114	0.0033477945829932035	2.56817118695369E-4	5.04462197437332E-4	3.5770955818283546E-4	8.942738954570886E-4	2.52231098718666E-4
115-119	0.0033936547827602337	2.751611986021811E-4	5.273922973208471E-4	3.989837379731626E-4	8.942738954570886E-4	2.52231098718666E-4
120-124	0.0034349289625505612	2.751611986021811E-4	5.273922973208471E-4	4.1274179790327166E-4	9.034459354104945E-4	2.52231098718666E-4
125-129	0.003531235382061324	2.797472185788841E-4	5.411503572509561E-4	4.1274179790327166E-4	9.355480752474157E-4	2.52231098718666E-4
130-134	0.0035725095618516513	3.210213983692113E-4	5.640804571344712E-4	4.1274179790327166E-4	0.0010135104148513668	2.52231098718666E-4
135-139	0.003618369761618681	3.2560741834591434E-4	5.732524970878773E-4	4.173278178799747E-4	0.0010639566345951004	2.52231098718666E-4
140-144	0.0036688159813624147	3.439514982527264E-4	5.732524970878773E-4	4.3567189778678675E-4	0.0010914727544553183	2.6140313867207204E-4
145-149	0.0037284342410595544	3.439514982527264E-4	5.732524970878773E-4	4.3567189778678675E-4	0.0011006447944087244	2.751611986021811E-4
150-154	0.0038247406605703173	3.668815981362415E-4	5.732524970878773E-4	4.494299577168958E-4	0.0011235748942922395	2.751611986021811E-4
155-159	0.0038339127005237233	3.668815981362415E-4	5.732524970878773E-4	4.815320975538169E-4	0.0011281609142689425	2.843332385555871E-4
160-164	0.003879772900290754	3.898116980197566E-4	5.732524970878773E-4	4.815320975538169E-4	0.0011510910141524576	2.980912984856962E-4
165-168	0.0038981169801975656	4.070092729323929E-4	5.961825969713924E-4	4.815320975538169E-4	0.0011694350940592696	3.03823823456575E-4
>>END_MODULE
