##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293681
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3594550
Total Bases	588.3 Mbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.750062733860986	39.0	37.0	39.0	35.0	39.0
2	39.369739744891575	40.0	39.0	41.0	36.0	41.0
3	39.4641429386154	40.0	39.0	41.0	37.0	41.0
4	39.502180245093264	40.0	39.0	41.0	37.0	41.0
5	39.528087521386546	40.0	39.0	41.0	37.0	41.0
6	39.515982807305505	40.0	39.0	41.0	37.0	41.0
7	39.54808334840244	40.0	39.0	41.0	37.0	41.0
8	39.525506113421706	40.0	39.0	41.0	37.0	41.0
9	39.50491494067408	40.0	39.0	41.0	37.0	41.0
10-14	39.41820522735808	40.0	39.0	41.0	36.4	41.0
15-19	39.2609519411331	40.0	38.6	41.0	36.0	41.0
20-24	39.04341962137124	40.0	38.0	41.0	35.4	41.0
25-29	38.74323628826975	40.0	38.0	41.0	35.0	41.0
30-34	38.48814727851887	40.0	37.4	41.0	34.4	41.0
35-39	37.89937243883101	39.0	35.8	40.0	33.6	41.0
40-44	37.394759010168166	38.2	35.0	40.0	33.2	41.0
45-49	36.56087204239752	36.4	35.0	39.4	33.0	40.8
50-54	35.7618709435117	35.2	34.2	38.0	33.0	39.8
55-59	35.131138584801995	35.0	34.0	36.4	32.2	39.0
60-64	34.60861877008249	34.8	33.8	35.6	31.8	37.4
65-69	34.51890840299899	35.0	33.8	35.0	31.8	37.0
70-74	34.53639498685509	35.0	34.0	35.0	32.0	37.0
75-79	34.52079186546299	35.0	34.0	35.0	32.0	37.2
80-84	34.52721364287602	35.0	33.6	35.0	32.0	38.0
85-89	34.40432515892115	34.8	33.0	35.0	31.2	38.2
90-94	37.61870780786041	39.6	36.4	40.2	32.0	40.2
95-99	38.09648743775985	40.0	36.8	41.0	32.2	41.0
100-104	37.94994015763889	40.0	36.0	41.0	32.0	41.0
105-109	37.764528121226945	40.0	35.6	41.0	32.0	41.0
110-114	37.536413928161615	39.0	35.0	40.2	32.0	41.0
115-119	37.23944074687735	38.8	35.0	40.0	31.6	41.0
120-124	36.87283009712538	38.0	34.4	40.0	31.0	41.0
125-129	36.42453464716532	37.2	33.8	39.8	30.8	40.8
130-134	35.937157584343915	36.0	34.0	39.2	30.6	40.6
135-139	35.49180015146062	35.0	33.2	38.6	30.2	40.2
140-144	35.107414792854144	35.0	33.4	37.2	31.0	39.6
145-149	34.54898522605058	35.0	33.0	36.0	30.0	38.4
150-154	34.11290407399649	35.0	33.0	35.0	30.0	36.6
155-159	33.85760169739443	35.0	33.0	35.0	30.0	36.0
160-164	33.71296761918016	35.0	33.0	35.0	30.0	35.0
165-169	33.64141224185104	35.0	33.0	35.0	30.0	35.0
170-174	33.60985423336166	35.0	33.0	35.0	30.0	35.0
175-179	33.41532339092728	34.8	32.8	35.0	29.8	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	2.0
28	60.0
29	927.0
30	5816.0
31	26269.0
32	88954.0
33	187541.0
34	316672.0
35	561644.0
36	948061.0
37	1057463.0
38	387048.0
39	14024.0
40	69.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.70331474037084	25.37942162440361	25.55769150519537	24.359572130030184
2	25.165959577694007	25.81040742234772	25.514626309273762	23.509006690684508
3	22.702341044080622	24.737644489574496	27.353938601493926	25.20607586485096
4	23.77165430999708	23.818447371715514	27.454006760234247	24.955891558053164
5	24.900335229722774	24.552085796553115	25.644183555660653	24.903395418063457
6	24.52429372244092	24.794870011545257	25.690587138863002	24.990249127150825
7	24.769136609589516	24.785939825569265	25.155972235745782	25.28895132909544
8	25.10620244536868	24.892935137917124	24.833205825485805	25.167656591228386
9	24.88812229625405	25.102279840313805	24.972360935304835	25.037236928127303
10-14	25.088342073416705	25.07018124660945	24.734639941021825	25.106836738952026
15-19	24.936400940312417	25.06671488781628	24.970018500229514	25.02686567164179
20-24	24.875236121350376	24.91040046737422	25.06626420553338	25.148099205742025
25-29	24.940790919586597	24.87878593982557	24.880599796914773	25.29982334367306
30-34	24.854543684188563	24.97973320721648	24.97779694259365	25.187926166001308
35-39	24.88544045847185	25.007553101222683	24.9228804718254	25.18412596848006
40-44	24.97910447761194	24.947567845766507	24.902788944374123	25.170538732247433
45-49	25.119973292901754	24.909654894214853	24.863234619076103	25.107137193807294
50-54	25.089950063290257	25.01331738326077	24.84144051411164	25.055292039337328
55-59	25.04317925748703	24.949748925456593	24.85699183486111	25.150079982195265
60-64	24.922413097606096	25.038683562615628	24.930375151270674	25.108528188507602
65-69	24.89874949576442	25.08991111543865	24.924989219791073	25.086350169005854
70-74	24.873021657787483	25.103609631247302	24.89874949576442	25.124619215200788
75-79	24.79488670348166	25.1300496585108	24.973345759552657	25.10171787845488
80-84	24.7780334116927	25.162387503303613	24.93726335702661	25.122315727977075
85-89	24.765041521191804	25.143803814107468	24.86552697834221	25.225627686358514
90-94	24.172728946357726	25.312881214946596	25.911279255555	24.603110583140676
95-99	24.498484459363358	25.1125633541442	25.19784061077078	25.191111575721663
100-104	24.659962309542138	25.332998177251852	24.81491583836559	25.19212367484042
105-109	24.610286919622	25.311569515620846	24.93521810743475	25.142925457322406
110-114	24.588572698250204	25.1895422773708	25.00759609995587	25.214288924423123
115-119	24.63026373390419	25.161461355708514	24.89175558523364	25.316519325153656
120-124	24.61464068841096	25.203313591934407	24.91090818561666	25.271137534037972
125-129	24.63467543199453	25.20095980482803	24.921489461157226	25.242875302020217
130-134	24.80315524333438	25.175032356118056	24.78096525657044	25.24084714397713
135-139	24.998559398568943	25.152648526007543	24.70766836086444	25.14112371455908
140-144	24.96911735138844	25.214559388254926	24.7623007882661	25.054022472090537
145-149	24.90918666341387	25.219746706881757	24.803181808523693	25.067884821180677
150-154	24.837658750399193	25.30263793179125	24.877214862261184	24.982488455548374
155-159	24.782571667256136	25.354141707151197	24.913352586247267	24.949934039345404
160-164	24.737802984056575	25.416405198843854	24.908585044336522	24.937206772763044
165-169	24.670518271057585	25.48473611104941	24.988273782287358	24.85647183560565
170-174	24.612226964989286	25.60013596355103	24.97842520036747	24.809211871092213
175-179	24.56962467342465	25.61691728759027	24.992980671683497	24.820477367301585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	3.0
19	9.5
20	23.0
21	54.5
22	109.5
23	212.5
24	375.0
25	590.0
26	918.5
27	1363.5
28	1987.5
29	2904.5
30	4134.5
31	5856.5
32	8227.0
33	11216.0
34	15133.5
35	20614.0
36	27822.5
37	36862.5
38	48232.5
39	62413.5
40	78850.0
41	97208.5
42	116266.5
43	134810.5
44	152208.5
45	167244.5
46	177762.5
47	184195.0
48	187406.5
49	187053.5
50	183346.5
51	176457.0
52	168218.5
53	158538.5
54	147136.0
55	135032.0
56	122805.5
57	110413.0
58	98926.0
59	88000.0
60	77280.0
61	67516.5
62	58956.0
63	51262.5
64	43990.5
65	37673.0
66	31560.0
67	25874.0
68	20972.0
69	16508.0
70	12642.5
71	9471.0
72	6988.5
73	4954.5
74	3398.0
75	2233.0
76	1407.0
77	877.0
78	524.0
79	303.0
80	161.0
81	73.0
82	34.5
83	16.0
84	6.0
85	2.5
86	1.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1464.0
92-93	3355.0
94-95	4936.0
96-97	6305.0
98-99	7781.0
100-101	9100.0
102-103	10246.0
104-105	11543.0
106-107	12811.0
108-109	14061.0
110-111	14933.0
112-113	15578.0
114-115	16310.0
116-117	16978.0
118-119	17565.0
120-121	18918.0
122-123	18968.0
124-125	20117.0
126-127	20008.0
128-129	20958.0
130-131	21721.0
132-133	23338.0
134-135	79697.0
136-137	81389.0
138-139	67506.0
140-141	70254.0
142-143	68487.0
144-145	73566.0
146-147	66919.0
148-149	66190.0
150-151	72079.0
152-153	68346.0
154-155	69809.0
156-157	60733.0
158-159	61887.0
160-161	59138.0
162-163	65810.0
164-165	61351.0
166-167	67274.0
168-169	62928.0
170-171	67251.0
172-173	67210.0
174-175	69377.0
176-177	73607.0
178-179	1786748.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.6444701751802
#Duplication Level	Percentage of total
1	91.88630977486977
2	6.759285876569882
3	0.7837528751941828
4	0.26356364258616694
5	0.13205527272064035
6	0.0771310737044208
7	0.05322485838962814
8	0.01651968151595626
9	0.0066992175138937465
>10	0.02145772693546081
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.7819894006203837E-5	0.0	0.0	0.0	0.0	0.0
2	5.563978801240767E-5	0.0	0.0	0.0	0.0	0.0
3	5.563978801240767E-5	0.0	0.0	0.0	0.0	0.0
4	5.563978801240767E-5	0.0	0.0	2.7819894006203837E-5	0.0	0.0
5	8.345968201861151E-5	0.0	0.0	2.7819894006203837E-5	0.0	0.0
6	8.345968201861151E-5	0.0	2.7819894006203837E-5	5.563978801240767E-5	0.0	0.0
7	8.345968201861151E-5	0.0	5.563978801240767E-5	5.563978801240767E-5	0.0	0.0
8	8.345968201861151E-5	0.0	5.563978801240767E-5	5.563978801240767E-5	0.0	0.0
9	8.345968201861151E-5	0.0	5.563978801240767E-5	5.563978801240767E-5	0.0	0.0
10-14	1.1127957602481535E-4	0.0	5.563978801240767E-5	5.563978801240767E-5	0.0	0.0
15-19	1.1127957602481535E-4	0.0	1.1127957602481535E-4	5.563978801240767E-5	0.0	0.0
20-24	1.1127957602481535E-4	1.1127957602481535E-5	1.6691936403722302E-4	5.563978801240767E-5	0.0	0.0
25-29	1.1127957602481535E-4	2.7819894006203837E-5	1.891752792421861E-4	5.563978801240767E-5	2.225591520496307E-5	0.0
30-34	1.1127957602481535E-4	3.894785160868537E-5	1.9473925804342686E-4	7.233172441612997E-5	2.7819894006203837E-5	0.0
35-39	1.1127957602481535E-4	8.345968201861151E-5	2.39251088453353E-4	1.3909947003101918E-4	5.563978801240767E-5	0.0
40-44	1.8917527924218607E-4	1.1127957602481535E-4	2.9489087646576066E-4	1.724833428384638E-4	1.2240753362729686E-4	1.6691936403722303E-5
45-49	2.670709824595568E-4	1.1127957602481535E-4	3.560946432794091E-4	1.9473925804342686E-4	1.5022742763350075E-4	5.00758092111669E-5
50-54	3.004548552670014E-4	1.1127957602481535E-4	4.284263676955391E-4	1.9473925804342686E-4	2.61507003658316E-4	5.563978801240767E-5
55-59	3.560946432794091E-4	1.1127957602481535E-4	4.729381981054652E-4	2.336871096521122E-4	3.282747492732053E-4	1.001516184223338E-4
60-64	4.284263676955391E-4	1.1127957602481535E-4	4.78502176906706E-4	2.503790460558345E-4	3.727865796831314E-4	1.6691936403722302E-4
65-69	4.729381981054652E-4	1.1127957602481535E-4	5.00758092111669E-4	2.503790460558345E-4	3.894785160868537E-4	1.8361130044094534E-4
70-74	6.454215409439289E-4	1.1127957602481535E-4	5.00758092111669E-4	2.503790460558345E-4	4.1173443129181674E-4	1.9473925804342686E-4
75-79	0.002893268976645199	1.1127957602481535E-4	5.118860497141506E-4	2.559430248570753E-4	4.284263676955391E-4	2.1143119444714918E-4
80-84	0.004000500758092111	1.1127957602481535E-4	5.50833901322836E-4	3.004548552670014E-4	4.618102405029837E-4	2.225591520496307E-4
85-89	0.004067268503707001	1.335354912297784E-4	5.897817529315213E-4	3.060188340682422E-4	5.00758092111669E-4	2.336871096521122E-4
90-94	0.004117344312918168	1.3909947003101918E-4	6.231656257389658E-4	3.060188340682422E-4	5.00758092111669E-4	2.503790460558345E-4
95-99	0.004117344312918168	1.3909947003101918E-4	6.621134773476514E-4	3.060188340682422E-4	5.00758092111669E-4	2.503790460558345E-4
100-104	0.004172984100930576	1.3909947003101918E-4	7.288812229625405E-4	3.060188340682422E-4	5.063220709129098E-4	2.503790460558345E-4
105-109	0.0042119319525392605	1.3909947003101918E-4	7.67829074571226E-4	3.060188340682422E-4	5.285779861178729E-4	2.503790460558345E-4
110-114	0.004256443782949187	1.5022742763350075E-4	7.789570321737074E-4	3.171467916707238E-4	5.285779861178729E-4	2.503790460558345E-4
115-119	0.004284263676955391	1.9473925804342686E-4	7.900849897761889E-4	3.6165862208064985E-4	5.452699225215952E-4	2.503790460558345E-4
120-124	0.004323211528564077	2.003032368446676E-4	8.345968201861149E-4	3.783505584843722E-4	5.842177741302807E-4	2.503790460558345E-4
125-129	0.004339903464967799	2.61507003658316E-4	8.345968201861149E-4	3.894785160868537E-4	6.843693925526143E-4	2.503790460558345E-4
130-134	0.004384415295377725	3.282747492732053E-4	8.735446717948004E-4	3.894785160868537E-4	6.954973501550959E-4	2.503790460558345E-4
135-139	0.0044734389561975774	3.3383872807444604E-4	9.347484386084488E-4	3.894785160868537E-4	7.17753265360059E-4	2.7263496126079753E-4
140-144	0.004556898638216189	3.3383872807444604E-4	9.458763962109304E-4	4.1729841009305747E-4	7.567011169687444E-4	2.7819894006203837E-4
145-149	0.004668178214241004	3.6165862208064985E-4	9.625683326146527E-4	4.339903464967799E-4	7.789570321737074E-4	2.7819894006203837E-4
150-154	0.004757201875060856	3.6165862208064985E-4	0.0010182081206270605	4.451183040992614E-4	7.789570321737074E-4	2.7819894006203837E-4
155-159	0.004840661557079467	3.6165862208064985E-4	0.001029336078229542	4.451183040992614E-4	7.845210109749482E-4	2.7819894006203837E-4
160-164	0.0048851733874893935	3.83914537285613E-4	0.001029336078229542	4.451183040992614E-4	8.067769261799112E-4	2.7819894006203837E-4
165-168	0.004938031186101181	3.894785160868537E-4	0.001029336078229542	4.451183040992614E-4	8.067769261799112E-4	3.060188340682422E-4
>>END_MODULE
