##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293702
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6445549
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-182
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.58934498830123	39.0	37.0	39.0	35.0	39.0
2	39.22106898884796	40.0	38.0	41.0	36.0	41.0
3	39.32449524470297	40.0	39.0	41.0	36.0	41.0
4	39.38515788181891	40.0	39.0	41.0	36.0	41.0
5	39.40685719711385	40.0	39.0	41.0	36.0	41.0
6	39.394580663338374	40.0	39.0	41.0	36.0	41.0
7	39.445690817027376	40.0	39.0	41.0	37.0	41.0
8	39.427519672878134	40.0	39.0	41.0	37.0	41.0
9	39.42349410422603	40.0	39.0	41.0	37.0	41.0
10-14	39.367357815447534	40.0	39.0	41.0	36.0	41.0
15-19	39.229550438605	40.0	38.8	41.0	36.0	41.0
20-24	39.07204587227558	40.0	38.0	41.0	35.8	41.0
25-29	38.89016249818285	40.0	38.0	41.0	35.0	41.0
30-34	38.7577657698359	40.0	38.0	41.0	35.0	41.0
35-39	38.397454305288804	40.0	37.0	40.4	34.2	41.0
40-44	38.14918676438578	39.6	36.4	40.0	34.0	41.0
45-49	37.57983644217118	38.6	35.2	40.0	33.2	41.0
50-54	36.91043354103739	37.2	35.0	40.0	33.0	41.0
55-59	36.22123812882347	36.0	34.4	39.0	33.0	40.2
60-64	35.461341555234476	35.0	33.8	37.2	32.0	39.6
65-69	35.030929498790556	35.0	34.0	36.2	32.0	39.0
70-74	34.74997226768426	35.0	34.0	36.0	32.0	37.4
75-79	34.560132240093125	35.0	34.0	35.2	32.0	37.0
80-84	34.47690873190165	35.0	33.8	35.0	32.0	37.0
85-89	34.32724517337468	34.8	33.0	35.0	31.6	37.2
90-94	36.738675216384635	38.6	35.0	39.0	31.4	39.0
95-99	38.24577385678138	40.0	37.0	41.0	32.0	41.0
100-104	38.21053903732768	40.0	37.0	41.0	32.0	41.0
105-109	38.11801049205472	40.0	37.0	41.0	32.0	41.0
110-114	37.97353997768409	40.0	36.0	41.0	32.0	41.0
115-119	37.7822160164713	40.0	35.8	41.0	32.0	41.0
120-124	37.566445122401646	39.4	35.0	40.0	31.8	41.0
125-129	37.35040180135528	39.0	35.0	40.0	31.0	41.0
130-134	36.88563524117215	38.4	34.4	39.8	31.0	40.6
135-139	36.81323127430673	38.0	34.0	40.0	31.0	41.0
140-144	36.60846876145049	37.0	34.0	40.0	31.0	41.0
145-149	36.28489957309286	36.2	34.0	39.6	31.0	41.0
150-154	35.739162095397546	35.4	34.0	38.8	31.0	40.4
155-159	35.16879336355062	35.0	34.0	37.0	31.0	39.4
160-164	34.67562297138411	35.0	34.0	36.0	31.0	38.0
165-169	34.291725305247134	35.0	34.0	35.2	31.0	36.8
170-174	34.054542569255766	35.0	33.4	35.0	31.0	36.0
175-179	33.93240906001207	35.0	33.0	35.0	31.0	36.0
180-182	33.65181642649598	34.666666666666664	32.666666666666664	35.0	30.333333333333332	35.333333333333336
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	154.0
29	1887.0
30	11579.0
31	48422.0
32	152958.0
33	320089.0
34	490534.0
35	748095.0
36	1201234.0
37	1685873.0
38	1427252.0
39	354627.0
40	2844.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.972371942250383	28.137091192697472	28.734805987821982	21.155730877230162
2	21.512333549865186	30.126277839172428	28.27743610358094	20.08395250738145
3	18.420990981528494	29.016287053282813	30.833215293220174	21.729506671968515
4	19.72741189307536	27.21290304363523	31.616251773122816	21.443433290166595
5	21.002772610990935	27.83720983270781	29.510876420301823	21.649141135999432
6	20.649722777687362	28.17234032353179	29.61904408763319	21.55889281114766
7	20.870867632842447	27.925813611842838	29.396440861748165	21.806877893566554
8	21.306501587374484	28.243738430969962	28.64604706286462	21.803712918790936
9	21.132474518462274	28.55961532524227	28.75531626553456	21.552593890760896
10-14	21.274718414211108	28.63839837382355	28.366679083503982	21.72020412846136
15-19	20.978852228103456	28.960378704746486	28.559255386934456	21.501513680215602
20-24	20.858257380403128	28.998873486184028	28.643018616412657	21.49985051700018
25-29	20.89461425240891	29.001346510592036	28.476004138669957	21.628035098329097
30-34	20.869102073384287	29.0698526999019	28.515200179224454	21.545845047489358
35-39	20.894161226607693	29.055855443810913	28.431384200166658	21.618599129414733
40-44	20.94309732188833	28.929079586548795	28.481592491190433	21.646230600372444
45-49	21.039517347552554	28.873245708007182	28.480404074191352	21.606832870248912
50-54	21.032712651784976	28.913127493096397	28.457703137467423	21.596456717651204
55-59	20.98823855035467	28.826463036740545	28.511985557785692	21.67331285511909
60-64	20.9063184532458	28.891552915042613	28.589452969793573	21.612675661918015
65-69	20.940310902919208	28.939502282893205	28.51091194869514	21.609274865492452
70-74	20.902598056426225	28.874412404591137	28.569144381650034	21.6538451573326
75-79	20.85979022112779	28.906114901926895	28.63789259844274	21.596202278502577
80-84	20.89076353309858	28.955383009267326	28.546983352387826	21.60687010524627
85-89	20.912710461125965	28.8836621985187	28.549071615156446	21.654555725198893
90-94	20.734335829252657	27.947910703619932	29.525167377092732	21.792586090034675
95-99	20.517380424988264	28.49886081268736	29.911277038417172	21.072481723907202
100-104	21.205647436639374	28.440698533875914	28.986248399176812	21.367405630307907
105-109	21.313188204251247	28.71784174200097	28.67341235810454	21.295557695643247
110-114	21.17930076441836	28.735174957078303	28.94537349782751	21.140150780675825
115-119	21.235495989210413	28.596449988504585	28.96576454011105	21.202289482173953
120-124	21.273130194510696	28.673096933072546	28.930360894952972	21.123411977463785
125-129	21.32933580436176	28.7374034857163	28.858308889271512	21.074951820650426
130-134	21.39964109583658	28.70342268824458	28.833695730481313	21.06324048543753
135-139	21.500000420109817	28.747546768725346	28.826171161141524	20.926281650023316
140-144	21.464811106754247	28.80980489804763	28.81290556009171	20.91247843510641
145-149	21.386869452301482	28.825633501840723	28.9221004767232	20.865396569134592
150-154	21.327110377837897	28.879954259552132	28.982447145675614	20.810488216934353
155-159	21.286112498276953	28.99995772361253	28.976450594371993	20.737479183738525
160-164	21.230274672086296	28.97573596834631	29.045497891148493	20.7484914684189
165-169	21.1103765593136	29.11683359958235	29.156715213970834	20.616074627133212
170-174	21.094255431949257	29.211077981623383	29.105064455416535	20.589602131010825
175-179	21.00869798967375	29.261911207108003	29.204431197058312	20.524959606159936
180-182	20.97898829280717	29.35691398862617	29.20471377929576	20.4593839392709
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.5
10	4.0
11	7.5
12	14.5
13	29.5
14	61.0
15	133.0
16	278.0
17	581.0
18	1270.5
19	2561.5
20	4792.5
21	8507.0
22	14325.0
23	22326.0
24	32526.0
25	45069.0
26	59510.5
27	75589.0
28	92660.5
29	109978.5
30	127596.0
31	144882.0
32	161473.0
33	177977.0
34	193738.5
35	208725.0
36	222197.5
37	234703.5
38	246886.0
39	256415.5
40	263915.0
41	268554.5
42	270232.0
43	268301.0
44	261828.5
45	253128.0
46	241103.5
47	226830.0
48	211720.0
49	194659.5
50	178051.5
51	162465.5
52	146891.0
53	132303.0
54	119169.5
55	107469.5
56	96673.5
57	86545.0
58	77595.5
59	69598.5
60	61575.0
61	54173.5
62	47524.0
63	41027.0
64	34809.0
65	29054.5
66	23921.5
67	19234.5
68	15052.5
69	11501.5
70	8566.5
71	6201.5
72	4364.0
73	2988.0
74	1960.5
75	1238.5
76	765.0
77	460.0
78	251.0
79	126.0
80	62.0
81	31.0
82	18.0
83	9.0
84	3.5
85	0.5
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-182	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1503.0
92-93	3674.0
94-95	5916.0
96-97	7823.0
98-99	9589.0
100-101	11037.0
102-103	12563.0
104-105	13659.0
106-107	15354.0
108-109	16682.0
110-111	17517.0
112-113	18375.0
114-115	19074.0
116-117	20169.0
118-119	22032.0
120-121	22288.0
122-123	22598.0
124-125	23208.0
126-127	24315.0
128-129	24862.0
130-131	25455.0
132-133	27070.0
134-135	80346.0
136-137	95307.0
138-139	105806.0
140-141	109086.0
142-143	110520.0
144-145	100183.0
146-147	102633.0
148-149	109984.0
150-151	98741.0
152-153	87358.0
154-155	96847.0
156-157	91669.0
158-159	85579.0
160-161	97762.0
162-163	81496.0
164-165	100304.0
166-167	88286.0
168-169	89658.0
170-171	89760.0
172-173	86108.0
174-175	91221.0
176-177	100540.0
178-179	110681.0
180-181	213982.0
182-183	3656929.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.7414386281838
#Duplication Level	Percentage of total
1	90.43606916892877
2	7.546897832435692
3	1.1423767956314732
4	0.28015774427548856
5	0.14735343292802505
6	0.11734534916504279
7	0.06488337197732366
8	0.04640232610529975
9	0.039321060510081114
>10	0.17919291804279425
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.1029164466828195E-5	1.5514582233414097E-5	0.0	1.5514582233414097E-5	0.0	0.0
2	0.0016445457167418942	1.5514582233414097E-5	0.0	1.5514582233414097E-5	1.5514582233414097E-5	0.0
3	0.0016600602989753084	3.1029164466828195E-5	1.5514582233414097E-5	1.5514582233414097E-5	3.1029164466828195E-5	0.0
4	0.0016600602989753084	4.654374670024229E-5	1.5514582233414097E-5	4.654374670024229E-5	3.1029164466828195E-5	0.0
5	0.0016600602989753084	4.654374670024229E-5	1.5514582233414097E-5	6.205832893365639E-5	3.1029164466828195E-5	0.0
6	0.0016600602989753084	4.654374670024229E-5	1.5514582233414097E-5	6.205832893365639E-5	4.654374670024229E-5	0.0
7	0.0016600602989753084	4.654374670024229E-5	3.1029164466828195E-5	7.757291116707049E-5	4.654374670024229E-5	0.0
8	0.0016600602989753084	4.654374670024229E-5	3.1029164466828195E-5	7.757291116707049E-5	6.205832893365639E-5	0.0
9	0.0016600602989753084	4.654374670024229E-5	3.1029164466828195E-5	9.308749340048458E-5	6.205832893365639E-5	0.0
10-14	0.0016600602989753084	6.516124538033921E-5	3.1029164466828195E-5	1.2411665786731278E-4	8.067582761375331E-5	0.0
15-19	0.0016755748812087225	8.067582761375331E-5	4.344083025355947E-5	1.7066040456755507E-4	1.0860207563389867E-4	0.0
20-24	0.0016755748812087225	1.0860207563389867E-4	5.274957959360793E-5	1.9548373614101765E-4	1.1791082497394713E-4	0.0
25-29	0.001697295296335502	1.0860207563389867E-4	6.826416182702203E-5	2.389245663945771E-4	1.5204290588745817E-4	0.0
30-34	0.0018989848653698856	1.0860207563389867E-4	7.757291116707049E-5	2.91674145988185E-4	2.1099831837443172E-4	0.0
35-39	0.002572317734300057	1.0860207563389867E-4	7.757291116707049E-5	3.1029164466828195E-4	2.8546831309481937E-4	0.0
40-44	0.003425619757137832	1.0860207563389867E-4	8.998457695380176E-5	3.164974775616476E-4	3.909674722820353E-4	0.0
45-49	0.0042634071977421945	1.1791082497394713E-4	1.3963124010072688E-4	3.630412242618899E-4	4.46819968322326E-4	0.0
50-54	0.004939842983119049	1.3963124010072688E-4	1.6755748812087226E-4	3.7545289004862115E-4	5.026724643626167E-4	2.7926248020145376E-5
55-59	0.005476647528395176	1.6135165522750662E-4	2.0168956903438327E-4	4.3751121898227753E-4	6.205832893365638E-4	3.1029164466828195E-5
60-64	0.005985525825651159	1.7066040456755507E-4	2.1720415126779734E-4	4.964666314692511E-4	7.881407774574361E-4	3.1029164466828195E-5
65-69	0.006190318311132226	1.8617498680096917E-4	2.3271873350121146E-4	5.337016288294449E-4	9.060516024313834E-4	3.1029164466828195E-5
70-74	0.008744018546752186	2.0168956903438327E-4	2.606449815213569E-4	5.647307932962731E-4	9.712128478117225E-4	3.1029164466828195E-5
75-79	0.05616589060140571	2.0168956903438327E-4	2.8236539664813656E-4	6.205832893365639E-4	0.0010643003412122072	4.344083025355947E-5
80-84	0.07601524711083571	2.0168956903438327E-4	3.0718872822159914E-4	6.423037044633437E-4	0.0011822111661861543	4.654374670024229E-5
85-89	0.07618901043184993	2.4202748284125989E-4	3.2270331045501324E-4	6.826416182702202E-4	0.001374591985880489	4.654374670024229E-5
90-94	0.07639070000088433	2.6685081441472246E-4	3.4132080913511014E-4	7.260824485237797E-4	0.0013807978187738547	4.654374670024229E-5
95-99	0.0766141099850455	2.7926248020145375E-4	3.4442372558179295E-4	7.881407774574361E-4	0.0013994153174539516	4.654374670024229E-5
100-104	0.07678787330605974	2.7926248020145375E-4	3.5683539136852424E-4	8.470961899444097E-4	0.0014335473983674628	4.654374670024229E-5
105-109	0.077029900788901	2.8546831309481937E-4	4.0337913806876653E-4	8.626107721778238E-4	0.001464576562834291	4.654374670024229E-5
110-114	0.07730295743620907	3.0718872822159914E-4	4.3440830253559467E-4	8.905370201979691E-4	0.0014769882286210221	4.654374670024229E-5
115-119	0.0775139557545835	3.2580622690169604E-4	4.3751121898227753E-4	9.556982655783083E-4	0.0015142232259812158	4.654374670024229E-5
120-124	0.07777460073610487	3.382178926884273E-4	4.747462163424714E-4	0.001027065343852013	0.0015731786384681895	4.654374670024229E-5
125-129	0.07856894734645567	3.78555806495304E-4	5.088782972559825E-4	0.0010705061741055726	0.0016538544660819425	4.654374670024229E-5
130-134	0.07974184976330177	3.8786455583535243E-4	5.554220439562246E-4	0.0011077411714657665	0.0017345302936956964	4.654374670024229E-5
135-139	0.0807130626111135	3.9096747228203524E-4	5.771424590830044E-4	0.0011542849181660087	0.0017903827897359868	4.654374670024229E-5
140-144	0.08160670254775815	4.0337913806876653E-4	5.926570413164185E-4	0.001185314082632837	0.0018834702831364715	4.654374670024229E-5
145-149	0.08241656374034237	4.0337913806876653E-4	6.112745399965154E-4	0.0012256519964397136	0.001954837361410176	4.654374670024229E-5
150-154	0.08337226200592067	4.0958497096213215E-4	6.205832893365639E-4	0.001253578244459859	0.0019889694423236874	4.654374670024229E-5
155-159	0.08407662403931768	4.3440830253559467E-4	6.267891222299295E-4	0.0012846074089266871	0.0020231015232371986	4.654374670024229E-5
160-164	0.08494544064438887	4.3440830253559467E-4	6.516124538033921E-4	0.001321842406286881	0.002063439437044075	4.654374670024229E-5
165-169	0.08576461058631313	4.3440830253559467E-4	6.516124538033921E-4	0.0013404599049669779	0.002094468601510903	4.654374670024229E-5
170-171	0.08627659180001579	4.344083025355947E-4	6.593697449200991E-4	0.0013652832365404406	0.0021254977659777314	4.654374670024229E-5
>>END_MODULE
