##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293703
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6026076
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-182
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.667160022542035	39.0	37.0	39.0	35.0	39.0
2	39.28564724374535	40.0	39.0	41.0	36.0	41.0
3	39.376456420396956	40.0	39.0	41.0	36.0	41.0
4	39.429225751550426	40.0	39.0	41.0	37.0	41.0
5	39.45316570849754	40.0	39.0	41.0	37.0	41.0
6	39.44313812172299	40.0	39.0	41.0	37.0	41.0
7	39.49486216237565	40.0	39.0	41.0	37.0	41.0
8	39.47476716191432	40.0	39.0	41.0	37.0	41.0
9	39.4631023571558	40.0	39.0	41.0	37.0	41.0
10-14	39.397588779165744	40.0	39.0	41.0	36.4	41.0
15-19	39.27569695436964	40.0	39.0	41.0	36.0	41.0
20-24	39.11583657424832	40.0	38.0	41.0	36.0	41.0
25-29	38.91198119638717	40.0	38.0	41.0	35.0	41.0
30-34	38.7874036437642	40.0	38.0	41.0	35.0	41.0
35-39	38.44793301644387	40.0	37.2	40.8	34.2	41.0
40-44	38.20896812453079	39.6	36.4	40.6	34.0	41.0
45-49	37.66207465687456	38.8	35.2	40.0	33.4	41.0
50-54	37.010753830519235	37.2	35.0	40.0	33.0	41.0
55-59	36.34328295892717	36.2	34.8	39.0	33.0	40.6
60-64	35.585144196654674	35.2	33.8	37.6	32.4	39.8
65-69	35.16346342130434	35.0	34.0	36.6	32.0	39.0
70-74	34.8963291203098	35.0	34.0	36.0	32.0	38.2
75-79	34.67722869741438	35.0	34.0	35.4	32.0	37.0
80-84	34.57909681192206	35.0	34.0	35.0	32.0	37.0
85-89	34.424768987314465	34.8	33.8	35.0	31.6	37.2
90-94	37.01799245209329	38.6	35.8	39.0	32.2	39.0
95-99	38.46996663057594	40.0	38.0	41.0	33.0	41.0
100-104	38.44965939339612	40.0	37.8	41.0	33.0	41.0
105-109	38.35191086154681	40.0	37.0	41.0	33.0	41.0
110-114	38.209021358917504	40.0	37.0	41.0	33.0	41.0
115-119	38.02466879831245	40.0	36.2	41.0	32.8	41.0
120-124	37.80273982416304	39.8	35.8	40.6	32.0	41.0
125-129	37.58892494840913	39.0	35.0	40.0	32.0	41.0
130-134	37.11436764183426	38.4	34.8	40.0	31.8	40.6
135-139	37.04878078812993	38.0	35.0	40.0	32.0	41.0
140-144	36.813278619162276	37.2	34.8	40.0	32.0	41.0
145-149	36.447253430092616	36.4	34.4	39.8	32.0	41.0
150-154	35.87391552183268	35.4	34.0	38.8	32.0	40.6
155-159	35.3025609981186	35.0	34.0	37.0	32.0	39.4
160-164	34.79404774073979	35.0	34.0	36.0	31.0	38.2
165-169	34.40733243993992	35.0	34.0	35.4	31.0	36.8
170-174	34.16742407483028	35.0	34.0	35.0	31.0	36.0
175-179	34.053709513661545	35.0	34.0	35.0	31.0	36.0
180-182	33.744995970038595	34.666666666666664	33.666666666666664	35.0	30.333333333333332	35.333333333333336
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	91.0
29	1363.0
30	8710.0
31	36652.0
32	115173.0
33	250312.0
34	416300.0
35	671826.0
36	1105261.0
37	1601617.0
38	1428011.0
39	386617.0
40	4142.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.031567474422825	28.088029424122762	28.692751302837866	21.187651798616546
2	21.583863197211585	30.03644494360841	28.2155585160227	20.164133343157307
3	18.52278331703749	28.917491249695495	30.77148379808021	21.78824163518681
4	19.76720837905131	27.186962129252933	31.56287773337077	21.482951758324987
5	21.0047798932506	27.803864405294586	29.47050452068643	21.72085118076838
6	20.700419311007696	28.085141972985404	29.591877035736026	21.622561680270877
7	20.860905172785742	27.915794623234092	29.364382394115175	21.858917809864995
8	21.34856911861052	28.152532427403838	28.602991399378304	21.895907054607346
9	21.195268695582335	28.506991946334566	28.69736458683893	21.600374771244173
10-14	21.33459651023319	28.57132568523862	28.317127098961247	21.77695070556694
15-19	21.026230004400873	28.905410419649535	28.510815329909544	21.557544246040045
20-24	20.901651422916007	28.953179481971354	28.600810875933195	21.544358219179447
25-29	20.928444978125068	28.942562290950196	28.455565445905428	21.673427285019304
30-34	20.909158795873132	29.019205864645585	28.471944263563888	21.599691075917395
35-39	20.94200936065194	29.01370311293784	28.39155364120864	21.65273388520158
40-44	20.9640502376671	28.878859144823267	28.468924056052398	21.68816656145724
45-49	21.07783572593509	28.81733320323209	28.43996989085435	21.66486117997848
50-54	21.065798041710725	28.874929556148977	28.412658585786172	21.646613816354122
55-59	21.019217148937386	28.750722692511676	28.49984301558759	21.730217142963347
60-64	20.94862062808368	28.837435837184927	28.554764327565735	21.659179207165657
65-69	20.974942898164578	28.8723773148563	28.497519779040292	21.655160007938832
70-74	20.933353645058574	28.825126002393596	28.5346683314316	21.70685202111623
75-79	20.904426031135355	28.86803618142221	28.58780738908703	21.639730398355415
80-84	20.93540141212955	28.91838071740217	28.496268550214104	21.649949320254176
85-89	20.954043725966947	28.838504525996683	28.519852720078536	21.68759902795783
90-94	20.811203598605953	27.896706080867972	29.47383854002117	21.818251780504905
95-99	20.586297303238283	28.44713764735759	29.849767279733864	21.116797769670267
100-104	21.282533055742686	28.377093741354308	28.924078383384856	21.416294819518146
105-109	21.38576126306274	28.649449921777176	28.625638886902117	21.339149928257967
110-114	21.252579524747127	28.68351401204435	28.894027232885133	21.169879230323396
115-119	21.320091631183463	28.558024395600007	28.905347898224548	21.21653607499198
120-124	21.34908378855188	28.64022203205766	28.871246547501713	21.13944763188875
125-129	21.384754001578738	28.688168816940674	28.813344402230463	21.113732779250128
130-134	21.454482446892882	28.645283388264435	28.798738003381835	21.101496161460844
135-139	21.547783596377982	28.687381084672044	28.774694232361515	20.990141086588455
140-144	21.514013782745643	28.761185616801583	28.76377623161521	20.961024368837567
145-149	21.442144235551154	28.765995892716074	28.875151253083896	20.916708618648872
150-154	21.394828251924356	28.824511304272306	28.94309389213192	20.83756655167142
155-159	21.337938635702983	28.94578655416214	28.93071591908652	20.78555889104836
160-164	21.28840540767095	28.924765258279166	29.007719808017164	20.77910952603272
165-169	21.173314558548654	29.065387278401904	29.10250048020822	20.65879768284122
170-174	21.161805534288483	29.146714849345383	29.04407789427343	20.647401722092702
175-179	21.077157170144144	29.2223460803205	29.138093952661208	20.56240279687415
180-182	21.06802035671522	29.309555172348333	29.13019818876897	20.49222628216748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	4.0
11	6.5
12	12.0
13	26.5
14	55.5
15	120.5
16	251.0
17	525.5
18	1129.5
19	2241.0
20	4269.0
21	7664.0
22	12958.5
23	20243.0
24	29547.0
25	40991.0
26	54348.5
27	69085.5
28	84582.0
29	100556.0
30	116794.5
31	133052.5
32	149002.5
33	164481.0
34	179211.5
35	193224.0
36	206855.0
37	219595.0
38	230728.0
39	240093.5
40	247188.0
41	251714.5
42	253108.5
43	251771.0
44	246479.0
45	237902.5
46	226921.0
47	213564.0
48	198860.5
49	183426.5
50	167795.5
51	152462.5
52	138076.5
53	124841.5
54	112521.5
55	101161.0
56	91210.0
57	81881.5
58	73504.5
59	65582.5
60	58035.5
61	51226.0
62	44853.0
63	38735.0
64	32838.0
65	27430.5
66	22578.0
67	18163.0
68	14366.5
69	10985.0
70	8149.5
71	6027.0
72	4327.5
73	2961.0
74	1938.5
75	1242.0
76	761.0
77	441.5
78	258.5
79	148.5
80	80.0
81	33.5
82	13.0
83	8.0
84	3.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-182	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1517.0
92-93	3616.0
94-95	5631.0
96-97	7477.0
98-99	9205.0
100-101	10549.0
102-103	11675.0
104-105	13091.0
106-107	14227.0
108-109	15798.0
110-111	16797.0
112-113	17526.0
114-115	18721.0
116-117	19068.0
118-119	20054.0
120-121	21170.0
122-123	21319.0
124-125	21749.0
126-127	24101.0
128-129	23667.0
130-131	24406.0
132-133	25715.0
134-135	69899.0
136-137	84826.0
138-139	97541.0
140-141	92894.0
142-143	90571.0
144-145	92799.0
146-147	78909.0
148-149	92739.0
150-151	85258.0
152-153	93321.0
154-155	94507.0
156-157	90784.0
158-159	79267.0
160-161	74464.0
162-163	90147.0
164-165	76423.0
166-167	87973.0
168-169	79882.0
170-171	89837.0
172-173	93379.0
174-175	94462.0
176-177	89330.0
178-179	92059.0
180-181	197736.0
182-183	3469990.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.06609230928781
#Duplication Level	Percentage of total
1	91.02513143981216
2	7.087639963715846
3	1.0420287900482124
4	0.30415634331368646
5	0.13423991399523816
6	0.09370624172831003
7	0.06613693628758395
8	0.04551340304372244
9	0.03628331169528915
>10	0.1651636563600011
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.6594546766419806E-5	6.637818706567922E-5	0.0	0.0	0.0	0.0
2	0.0015598873960434617	6.637818706567922E-5	0.0	0.0	0.0	0.0
3	0.0015598873960434617	9.956728059851884E-5	0.0	0.0	0.0	0.0
4	0.0015764819428098816	1.1616182736493865E-4	0.0	3.318909353283961E-5	0.0	0.0
5	0.0015764819428098816	1.3275637413135845E-4	0.0	3.318909353283961E-5	0.0	0.0
6	0.0015764819428098816	1.3275637413135845E-4	0.0	3.318909353283961E-5	3.318909353283961E-5	0.0
7	0.0015764819428098816	1.3275637413135845E-4	0.0	4.978364029925942E-5	3.318909353283961E-5	0.0
8	0.0015764819428098816	1.3275637413135845E-4	0.0	4.978364029925942E-5	3.318909353283961E-5	0.0
9	0.0015930764895763015	1.3275637413135845E-4	0.0	4.978364029925942E-5	3.318909353283961E-5	0.0
10-14	0.0016030332176361535	1.360752834846424E-4	4.646473094597546E-5	7.633491512553112E-5	5.310254965254338E-5	0.0
15-19	0.0016196277644025731	1.5930764895763014E-4	1.0952400865837071E-4	1.2279964607150656E-4	6.637818706567922E-5	0.0
20-24	0.001636222311168993	1.7590219572404995E-4	1.1616182736493865E-4	1.8917783313718582E-4	7.965382447881507E-5	0.0
25-29	0.0016594546766419804	1.991345611970377E-4	1.1616182736493865E-4	2.4891820149629707E-4	1.028861899518028E-4	0.0
30-34	0.001905053968784994	1.991345611970377E-4	1.3275637413135845E-4	2.6219383890943294E-4	1.69264377017482E-4	0.0
35-39	0.0026883165761600086	1.991345611970377E-4	1.5598873960434616E-4	2.8210729502913673E-4	2.6551274826271695E-4	0.0
40-44	0.003508087186421147	1.991345611970377E-4	1.991345611970377E-4	2.887451137357047E-4	3.6508002886123577E-4	0.0
45-49	0.004244885062850186	1.991345611970377E-4	2.157291079634575E-4	3.385287540349641E-4	5.376633152320016E-4	0.0
50-54	0.0047460403751960634	2.0245347055032164E-4	2.323236547298773E-4	3.7835566627437164E-4	6.339116864772366E-4	0.0
55-59	0.005207368775302536	2.157291079634575E-4	2.987018417955565E-4	4.28139306573631E-4	6.936520548363478E-4	0.0
60-64	0.005715161906354982	2.157291079634575E-4	3.5512330080138385E-4	4.480527626933348E-4	7.567113325487433E-4	6.637818706567922E-6
65-69	0.005954123379791426	2.3564256408316122E-4	3.982691223940754E-4	4.978364029925942E-4	8.064949728480025E-4	1.6594546766419806E-5
70-74	0.00894114179774699	2.4891820149629707E-4	4.181825785137791E-4	5.575767713517055E-4	8.994244347399535E-4	2.655127482627169E-5
75-79	0.062395495841738466	2.621938389094329E-4	4.480527626933347E-4	5.708524087648414E-4	9.127000721530894E-4	3.318909353283961E-5
80-84	0.08447951867848995	2.887451137357046E-4	4.746040375196065E-4	6.637818706567922E-4	0.001032180808871312	3.318909353283961E-5
85-89	0.08466205869292058	3.1529638856197633E-4	5.14430949759014E-4	6.969709641896319E-4	0.0012180397326552136	3.318909353283961E-5
90-94	0.08482800416058477	3.4516657274153193E-4	5.409822245852857E-4	6.969709641896319E-4	0.0012412720981282016	3.318909353283961E-5
95-99	0.08501054417501537	3.717178475678037E-4	5.675334994115574E-4	6.969709641896319E-4	0.001287736829074177	3.318909353283961E-5
100-104	0.08525946237651168	3.982691223940754E-4	5.808091368246932E-4	7.301600577224714E-4	0.0013242448319603004	3.318909353283961E-5
105-109	0.08549510494059484	4.148636691604952E-4	5.808091368246932E-4	8.098138822012866E-4	0.0013441582880800043	3.318909353283961E-5
110-114	0.08577721223562398	4.31458215926915E-4	5.97403683591113E-4	9.45889165685929E-4	0.0013474771974332884	3.318909353283961E-5
115-119	0.08620203263284432	4.31458215926915E-4	6.538251425969404E-4	0.0010056295340450405	0.0014171742938522513	3.318909353283961E-5
120-124	0.08647418319981362	4.546905813999027E-4	7.135655109560517E-4	0.001032180808871312	0.0014868713902712147	3.318909353283961E-5
125-129	0.0872010243481828	4.8124185622617436E-4	7.334789670757554E-4	0.0010786455398172873	0.0015632063053967458	3.318909353283961E-5
130-134	0.08817346478869499	4.8787967493274227E-4	7.467546044888913E-4	0.0011284291801165467	0.0016395412205222767	3.318909353283961E-5
135-139	0.08931516960622468	5.14430949759014E-4	7.832626073750149E-4	0.0011782128204158061	0.0017158761356478082	3.318909353283961E-5
140-144	0.09059294970723901	5.14430949759014E-4	8.131327915545706E-4	0.0012213586420084977	0.001871864875252154	3.318909353283961E-5
145-149	0.09187072980825334	5.310254965254338E-4	8.49640794440694E-4	0.0012611855542479053	0.0019415619716711171	3.318909353283961E-5
150-154	0.09265731132498163	5.310254965254338E-4	8.728731599136818E-4	0.001287736829074177	0.001984707793263809	3.318909353283961E-5
155-159	0.09331113646757858	5.310254965254338E-4	9.127000721530894E-4	0.0013142881039004488	0.0020245347055032163	3.318909353283961E-5
160-164	0.09430349036421048	5.310254965254338E-4	9.160189815063732E-4	0.0013308826506668683	0.00206104270838934	3.318909353283961E-5
165-169	0.09506352060611249	5.310254965254338E-4	9.292946189195092E-4	0.0013673906535529921	0.0021041885299820312	3.318909353283961E-5
170-171	0.09558458937457809	5.310254965254338E-4	9.292946189195092E-4	0.0013856446549960538	0.002124101986101735	3.318909353283961E-5
>>END_MODULE
