##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293704
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7763738
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-182
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.71087857421258	39.0	37.0	39.0	35.0	39.0
2	39.36371436027336	40.0	39.0	41.0	36.0	41.0
3	39.44619035315205	40.0	39.0	41.0	37.0	41.0
4	39.49004938085237	40.0	39.0	41.0	37.0	41.0
5	39.50987604166962	40.0	39.0	41.0	37.0	41.0
6	39.49725068002037	40.0	39.0	41.0	37.0	41.0
7	39.53425978568571	40.0	39.0	41.0	37.0	41.0
8	39.522142169145845	40.0	39.0	41.0	37.0	41.0
9	39.51672042513542	40.0	39.0	41.0	37.0	41.0
10-14	39.46277777534481	40.0	39.0	41.0	36.8	41.0
15-19	39.32344777734643	40.0	39.0	41.0	36.0	41.0
20-24	39.16913079756169	40.0	38.2	41.0	36.0	41.0
25-29	38.96938029078261	40.0	38.0	41.0	35.0	41.0
30-34	38.852683926222134	40.0	38.0	41.0	35.0	41.0
35-39	38.50396824313237	40.0	37.2	41.0	34.4	41.0
40-44	38.26280655014376	39.8	36.6	41.0	34.0	41.0
45-49	37.71333720947307	38.8	35.2	40.0	33.6	41.0
50-54	37.06706388082648	37.4	35.0	40.0	33.0	41.0
55-59	36.39779670565905	36.2	35.0	39.0	33.0	40.6
60-64	35.649157841235755	35.2	33.8	37.8	32.6	39.8
65-69	35.223330462722984	35.0	34.0	36.6	32.6	39.0
70-74	34.93294384740959	35.0	34.0	36.0	32.2	38.2
75-79	34.712130960627476	35.0	34.0	35.4	32.0	37.0
80-84	34.621149425701894	35.0	34.0	35.0	32.0	37.0
85-89	34.476182426557926	34.8	33.8	35.0	31.6	37.2
90-94	37.255057789521196	39.0	36.4	39.0	32.6	39.0
95-99	38.723937038743436	40.0	38.0	41.0	33.0	41.0
100-104	38.67672128448894	40.0	38.0	41.0	33.0	41.0
105-109	38.57945272737617	40.0	38.0	41.0	33.0	41.0
110-114	38.43505217984278	40.0	37.6	41.0	33.0	41.0
115-119	38.25048622344542	40.0	37.0	41.0	33.0	41.0
120-124	38.02813025302034	40.0	36.2	41.0	33.0	41.0
125-129	37.80506093983513	39.6	35.8	40.8	32.6	41.0
130-134	37.31930007904045	38.6	35.0	40.4	32.0	40.8
135-139	37.24641312569512	38.6	35.0	40.0	32.0	41.0
140-144	37.00278796398173	37.8	35.0	40.0	32.0	41.0
145-149	36.60607093466695	36.6	35.0	39.8	32.0	41.0
150-154	36.00712593583212	35.6	34.2	38.8	32.0	40.6
155-159	35.413395522597796	35.0	34.0	37.0	32.0	39.4
160-164	34.89003342214362	35.0	34.0	36.0	32.0	38.2
165-169	34.48702088258238	35.0	34.0	35.4	32.0	36.8
170-174	34.23527775022934	35.0	34.0	35.0	32.0	36.0
175-179	34.098177713894245	35.0	34.0	35.0	31.4	36.0
180-182	33.795509002626716	34.666666666666664	33.666666666666664	35.0	30.333333333333332	35.666666666666664
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	110.0
29	1688.0
30	10063.0
31	41740.0
32	132840.0
33	287604.0
34	481387.0
35	808645.0
36	1383722.0
37	2091676.0
38	1958949.0
39	558873.0
40	6440.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.084001289069775	27.983813982388376	28.620891122291862	21.31129360624998
2	21.65092382045865	29.987050052436082	28.16258096293306	20.199445164172207
3	18.570307241176867	28.873759006293103	30.695484571993543	21.86044918053649
4	19.87372577487803	27.090275328714082	31.465770740846743	21.57022815556115
5	21.133904827803306	27.685491189939693	29.363020751086655	21.817583231170346
6	20.747969599180188	28.034704416867235	29.523407410193386	21.693918573759184
7	20.99191137052796	27.816201422562177	29.262347595964727	21.929539610945138
8	21.43436576556293	28.089304404656623	28.518891286645687	21.957438543134764
9	21.25809758134548	28.419956984638066	28.648532446612702	21.673412987403747
10-14	21.38776707817806	28.516814967223265	28.2438639737714	21.851553980827276
15-19	21.096768077438984	28.840421971993386	28.430987753579522	21.6318221969881
20-24	20.988021491709276	28.86494881718059	28.53053516231485	21.61649452879528
25-29	21.017849906836116	28.873524583132504	28.35810275926364	21.750522750767736
30-34	20.984350579579065	28.951103192817683	28.404067731291292	21.660478496311956
35-39	21.02332149796915	28.931429679878427	28.31066684630522	21.7345819758472
40-44	21.055383373318367	28.82665283140673	28.363612991576996	21.754350803697907
45-49	21.136468026097738	28.74622765477145	28.38645250522364	21.73085181390717
50-54	21.14523184579387	28.790180709343876	28.346433122807596	21.71815432205466
55-59	21.106863729816748	28.69800603781323	28.398209728355077	21.796920504014945
60-64	21.01762578799027	28.766753849756395	28.47926862034757	21.736351741905768
65-69	21.055339579980675	28.807695468342697	28.418094479746742	21.71887047192989
70-74	21.02533599150306	28.737054238563946	28.436392366666674	21.801217403266314
75-79	20.98135460006507	28.797069143755238	28.521405539445045	21.700170716734647
80-84	21.016239857656196	28.845409260333106	28.416319561530802	21.722031320479903
85-89	21.042497827721647	28.75437579166118	28.442763009261775	21.760363371355396
90-94	20.898523248531113	27.794562073670203	29.402144551385827	21.904770126412853
95-99	20.66578235543476	28.375515240735893	29.79677951592713	21.161922887902215
100-104	21.396175705387364	28.2689971626014	28.85047573823622	21.484351393775015
105-109	21.505317680210855	28.574053576828973	28.524256599382063	21.39637214357811
110-114	21.372907376270195	28.587264941341946	28.792702415679948	21.247125266707908
115-119	21.399889159764836	28.45473132695373	28.83999904369819	21.305380469583245
120-124	21.44609014318259	28.54690780466421	28.79354495108684	21.213457101066357
125-129	21.485560544603427	28.624624596265935	28.70299445176192	21.18682040736872
130-134	21.549117822709363	28.547914542835308	28.723113110826898	21.179854523628432
135-139	21.629443383425397	28.59588458958595	28.719464547068043	21.05520747992061
140-144	21.604004334868286	28.673848952952884	28.68629491464857	21.03585179753026
145-149	21.53120806972742	28.661550666890072	28.81460308593632	20.99263817744619
150-154	21.452851433551615	28.761428293567175	28.87530394199334	20.91041633088787
155-159	21.416798514439773	28.856233568759798	28.871211380646137	20.855756536154292
160-164	21.364012207253534	28.852025830818256	28.92030214638588	20.86365981554233
165-169	21.246093666091653	28.972555034048053	29.026277435594537	20.755073864265754
170-174	21.271806671549363	29.060092636641066	28.96364066002	20.70446003178957
175-179	21.166177180436527	29.11901011972125	29.066079888122143	20.648732811720087
180-182	21.169643574486088	29.206077685378318	29.05196257601598	20.57231616411961
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	3.0
11	5.0
12	14.0
13	28.0
14	63.5
15	160.5
16	336.5
17	658.0
18	1408.0
19	2852.0
20	5500.0
21	9857.5
22	16370.0
23	25389.0
24	37158.5
25	51712.5
26	68737.0
27	87425.5
28	107255.0
29	127304.0
30	147790.0
31	168811.0
32	188777.5
33	207957.5
34	227504.0
35	245888.0
36	262953.5
37	279074.0
38	293307.0
39	305239.5
40	315270.0
41	322519.0
42	325489.5
43	323512.0
44	316905.0
45	306504.5
46	292782.0
47	276345.0
48	258140.5
49	239066.0
50	219222.0
51	198891.0
52	179867.0
53	163532.5
54	148028.0
55	133436.5
56	120464.5
57	108285.5
58	97255.0
59	87205.0
60	77306.5
61	68323.0
62	60017.5
63	51932.5
64	44135.5
65	36870.5
66	30352.0
67	24448.0
68	19220.5
69	14710.0
70	11063.0
71	8168.0
72	5747.0
73	3892.5
74	2607.0
75	1687.5
76	1009.5
77	592.5
78	364.5
79	207.0
80	104.0
81	54.5
82	29.0
83	15.5
84	5.0
85	1.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-182	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1905.0
92-93	4537.0
94-95	6670.0
96-97	8810.0
98-99	10678.0
100-101	13043.0
102-103	14144.0
104-105	15548.0
106-107	16337.0
108-109	19068.0
110-111	19926.0
112-113	20768.0
114-115	21740.0
116-117	22572.0
118-119	24062.0
120-121	24406.0
122-123	25465.0
124-125	25525.0
126-127	26955.0
128-129	27900.0
130-131	28855.0
132-133	29670.0
134-135	83891.0
136-137	99384.0
138-139	120193.0
140-141	103361.0
142-143	108273.0
144-145	111980.0
146-147	102651.0
148-149	94585.0
150-151	93417.0
152-153	109203.0
154-155	116684.0
156-157	91251.0
158-159	95978.0
160-161	93671.0
162-163	90781.0
164-165	92010.0
166-167	97827.0
168-169	95967.0
170-171	114625.0
172-173	94585.0
174-175	118028.0
176-177	96570.0
178-179	110065.0
180-181	248093.0
182-183	4772081.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.9732081450087
#Duplication Level	Percentage of total
1	89.12620818501503
2	8.414367727272595
3	1.3315635142806919
4	0.41855415839150406
5	0.17526841766286078
6	0.09077825262007963
7	0.07163639197319883
8	0.0553783262988736
9	0.05362994508946358
>10	0.2612494894520827
>50	0.0013655919436151437
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.5760786878691682E-5	2.5760786878691682E-5	1.2880393439345841E-5	1.2880393439345841E-5	0.0	0.0
2	0.0016358099667969218	3.8641180318037525E-5	1.2880393439345841E-5	1.2880393439345841E-5	0.0	0.0
3	0.0016358099667969218	5.1521573757383364E-5	1.2880393439345841E-5	1.2880393439345841E-5	0.0	0.0
4	0.0016358099667969218	6.440196719672921E-5	2.5760786878691682E-5	1.2880393439345841E-5	0.0	0.0
5	0.0016358099667969218	6.440196719672921E-5	2.5760786878691682E-5	2.5760786878691682E-5	0.0	0.0
6	0.0016358099667969218	6.440196719672921E-5	2.5760786878691682E-5	2.5760786878691682E-5	0.0	0.0
7	0.0016358099667969218	6.440196719672921E-5	2.5760786878691682E-5	2.5760786878691682E-5	0.0	0.0
8	0.0016358099667969218	6.440196719672921E-5	3.8641180318037525E-5	3.8641180318037525E-5	0.0	0.0
9	0.0016486903602362676	6.440196719672921E-5	5.1521573757383364E-5	3.8641180318037525E-5	1.2880393439345841E-5	0.0
10-14	0.0016486903602362679	6.440196719672921E-5	5.152157375738337E-5	5.4097652445252536E-5	1.545647212721501E-5	0.0
15-19	0.0016564185962998751	7.470628194820588E-5	5.66737311331217E-5	7.470628194820588E-5	2.5760786878691685E-5	0.0
20-24	0.001674451147114959	7.728236063607505E-5	7.21302032603367E-5	1.4941256389641177E-4	5.66737311331217E-5	0.0
25-29	0.0016873315405543051	8.243451801181338E-5	1.1592354095411257E-4	1.9062982290231844E-4	1.0304314751476674E-4	0.0
30-34	0.0018650809700172777	9.016275407542089E-5	1.4168432783280425E-4	2.1639060978101014E-4	1.622929573357576E-4	0.0
35-39	0.0023622641567760273	9.016275407542089E-5	1.622929573357576E-4	2.75640419602001E-4	2.2154276715674847E-4	0.0
40-44	0.0029444579402344595	9.273883276329005E-5	1.9578198027805677E-4	3.2716199335938435E-4	3.297380720472535E-4	0.0
45-49	0.003493162700750592	1.0819530489050507E-4	2.0608629502953348E-4	4.0702043268332853E-4	4.2505298349841274E-4	0.0
50-54	0.0040727804055211555	1.1592354095411257E-4	2.3699923928396346E-4	4.7657455725579604E-4	5.152157375738337E-4	0.0
55-59	0.004629213402100895	1.1592354095411257E-4	2.550317900990477E-4	5.20367894949572E-4	5.847698621463012E-4	5.152157375738336E-6
60-64	0.005033657756096355	1.3653217045706592E-4	2.6533610485052434E-4	6.079545703371236E-4	6.800847735974605E-4	1.2880393439345843E-5
65-69	0.005298993860946879	1.4683648520854258E-4	2.833686556656085E-4	6.929651670368063E-4	7.290302686669746E-4	1.2880393439345843E-5
70-74	0.008233147486429861	1.622929573357576E-4	3.245859146715152E-4	7.522149768577971E-4	8.011604719273113E-4	1.2880393439345843E-5
75-79	0.05920859256198496	1.6744511471149592E-4	3.6065101630168355E-4	7.985843932394422E-4	8.990514620663397E-4	2.3184708190822513E-5
80-84	0.08092493590072206	1.9578198027805677E-4	3.735314097410294E-4	8.11464786678788E-4	0.0010613444194020973	3.8641180318037525E-5
85-89	0.08111556572362437	2.421513966597018E-4	3.992921966197211E-4	8.475298883089564E-4	0.00123909384886507	3.8641180318037525E-5
90-94	0.08125982613014504	2.679121835383935E-4	4.147486687469361E-4	8.990514620663397E-4	0.0012700067931195	3.8641180318037525E-5
95-99	0.08149424929074114	3.1428159992003853E-4	4.2505298349841274E-4	9.479969571358539E-4	0.0012751589504952383	3.8641180318037525E-5
100-104	0.08163335753988607	3.22009835983646E-4	4.2505298349841274E-4	9.866381374538913E-4	0.0012751589504952383	3.8641180318037525E-5
105-109	0.08193475874636677	3.3489022942299185E-4	4.379333769377586E-4	0.0010381597112112749	0.0012983436586860608	3.8641180318037525E-5
110-114	0.08216660582827498	3.4261846548659934E-4	4.7399847856792693E-4	0.0010974095210322654	0.0013704738619463974	3.8641180318037525E-5
115-119	0.08242163761837404	3.4777062286233773E-4	5.04911422822357E-4	0.0011360507013503031	0.0014091150422644353	3.8641180318037525E-5
120-124	0.08270758235272753	3.6065101630168355E-4	5.69313390019086E-4	0.0012210612980499858	0.001504429953715594	3.8641180318037525E-5
125-129	0.08338766712632498	3.735314097410294E-4	6.234110424643386E-4	0.001288039343934584	0.0015507993700972392	3.8641180318037525E-5
130-134	0.08446704409654215	3.735314097410294E-4	6.311392785279462E-4	0.0013447130750677057	0.0015817123143516694	3.8641180318037525E-5
135-139	0.08549232341431408	3.8898788186824437E-4	6.388675145915537E-4	0.0013704738619463974	0.0016564185962998751	3.8641180318037525E-5
140-144	0.08661291764353718	4.0702043268332864E-4	6.59476144094507E-4	0.0013833542553857432	0.001749157429063165	3.8641180318037525E-5
145-149	0.08759955578099107	4.2505298349841274E-4	6.800847735974602E-4	0.0014091150422644349	0.001841896261826455	3.8641180318037525E-5
150-154	0.08832858604965806	4.2505298349841274E-4	7.084216391640213E-4	0.0014426040652067342	0.001903722150335315	3.8641180318037525E-5
155-159	0.08899321435112828	4.379333769377586E-4	7.290302686669747E-4	0.0014632126947096875	0.0019500915667169604	3.8641180318037525E-5
160-164	0.0899618199377671	4.73998478567927E-4	7.496388981699279E-4	0.0014992777963398559	0.0020325260847287737	4.3793337693775855E-5
165-169	0.09088663218671214	4.765745572557961E-4	7.80551842424358E-4	0.0015353428979700242	0.0020608629502953347	5.152157375738337E-5
170-171	0.09141215223903744	4.765745572557961E-4	7.985843932394422E-4	0.0015649678028805197	0.0020673031470150073	5.1521573757383364E-5
>>END_MODULE
