##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293705
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7569572
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-182
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.689998589088	39.0	37.0	39.0	35.0	39.0
2	39.32696960937818	40.0	39.0	41.0	36.0	41.0
3	39.409239914753435	40.0	39.0	41.0	36.0	41.0
4	39.45530368163484	40.0	39.0	41.0	37.0	41.0
5	39.47115173750907	40.0	39.0	41.0	37.0	41.0
6	39.4545983577407	40.0	39.0	41.0	37.0	41.0
7	39.506568667290566	40.0	39.0	41.0	37.0	41.0
8	39.47411386007029	40.0	39.0	41.0	37.0	41.0
9	39.469563140425905	40.0	39.0	41.0	37.0	41.0
10-14	39.425968311022075	40.0	39.0	41.0	36.6	41.0
15-19	39.299167218437184	40.0	39.0	41.0	36.0	41.0
20-24	39.13505508633777	40.0	38.0	41.0	36.0	41.0
25-29	38.94305271156678	40.0	38.0	41.0	35.0	41.0
30-34	38.833226502106065	40.0	38.0	41.0	35.0	41.0
35-39	38.49497625493225	40.0	37.2	41.0	34.2	41.0
40-44	38.26013005755147	39.8	36.6	41.0	34.0	41.0
45-49	37.71584768597221	38.8	35.4	40.0	33.6	41.0
50-54	37.06839681292417	37.4	35.0	40.0	33.0	41.0
55-59	36.39857268019909	36.2	35.0	39.0	33.0	40.6
60-64	35.65022072582176	35.2	34.0	37.8	32.6	39.8
65-69	35.2139906985494	35.0	34.0	36.6	32.2	39.0
70-74	34.92604321618184	35.0	34.0	36.0	32.0	38.2
75-79	34.703109766311755	35.0	34.0	35.4	32.0	37.0
80-84	34.60909396198358	35.0	34.0	35.0	32.0	37.0
85-89	34.45171124602554	34.8	33.8	35.0	31.6	37.2
90-94	37.23546179450824	39.0	36.4	39.0	32.6	39.0
95-99	38.66684873090858	40.0	38.0	41.0	33.0	41.0
100-104	38.631258212617624	40.0	38.0	41.0	33.0	41.0
105-109	38.52809357999517	40.0	38.0	41.0	33.0	41.0
110-114	38.39392809265447	40.0	37.4	41.0	33.0	41.0
115-119	38.207753083364295	40.0	37.0	41.0	33.0	41.0
120-124	37.987477764106444	40.0	36.0	41.0	33.0	41.0
125-129	37.76335332752116	39.2	35.6	40.6	32.2	41.0
130-134	37.28080271980135	38.6	35.0	40.2	32.0	40.8
135-139	37.222472503459116	38.6	35.0	40.0	32.0	41.0
140-144	36.97197044282974	37.8	35.0	40.0	32.0	41.0
145-149	36.608323068589286	36.6	35.0	39.8	32.0	41.0
150-154	36.01221403021826	35.6	34.0	39.0	32.0	40.6
155-159	35.42280731699435	35.0	34.0	37.2	32.0	39.6
160-164	34.89456191698053	35.0	34.0	36.0	32.0	38.2
165-169	34.49440533636571	35.0	34.0	35.4	32.0	36.8
170-174	34.24194375064139	35.0	34.0	35.0	32.0	36.0
175-179	34.11877084250464	35.0	34.0	35.0	32.0	36.0
180-182	33.8114869346222	34.666666666666664	33.666666666666664	35.0	31.333333333333332	35.666666666666664
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	85.0
29	1423.0
30	9310.0
31	38988.0
32	125643.0
33	283403.0
34	488322.0
35	808004.0
36	1367294.0
37	2028981.0
38	1880989.0
39	531514.0
40	5615.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.020174984794387	28.09059746046408	28.663839910631673	21.225387644109865
2	21.561166205962504	30.07413100767124	28.196125223460456	20.168577562905803
3	18.464888107280043	28.993184819432326	30.759017815009887	21.782909258277748
4	19.803444633329335	27.161760268612277	31.564690843815214	21.47010425424317
5	21.054321169017218	27.755347330073615	29.41345957208677	21.776871928822395
6	20.701474270936323	28.101826629035298	29.56278109251091	21.633918007517465
7	20.89067915596813	27.934842286987955	29.329187964656388	21.845290592387524
8	21.357587985159533	28.173204508788608	28.580942225002946	21.888265281048916
9	21.18242616623503	28.460023895670723	28.720012175060887	21.637537763033365
10-14	21.327269230017233	28.565128913497357	28.321878700671583	21.78572315581383
15-19	21.030473057129253	28.904506093607406	28.497674637350702	21.567346211912643
20-24	20.902938765890593	28.946408066400586	28.59514646270621	21.555506705002607
25-29	20.937281526617358	28.92919705367754	28.44246676033995	21.691054659365154
30-34	20.924179068512725	29.017344705882977	28.45920746906166	21.599268756542642
35-39	20.941915870540633	29.010205596828985	28.387095069575928	21.66078346305445
40-44	20.981104347775542	28.88668210038824	28.438408406710447	21.693805145125776
45-49	21.08794262079811	28.821999447260694	28.430006346461862	21.660051585479337
50-54	21.075545618695482	28.87429302475754	28.401986268180025	21.64817508836695
55-59	21.037392867126435	28.76591437402273	28.464180009120728	21.732512749730105
60-64	20.968894938841984	28.83224044899764	28.546718361355172	21.6521462508052
65-69	20.991694114277532	28.851359099299138	28.485483723518318	21.671463062905012
70-74	20.93985234568084	28.806307674991398	28.530817858658324	21.723022120669437
75-79	20.915235366015413	28.850835952151588	28.592908555463904	21.6410201263691
80-84	20.950637631823835	28.910781217220737	28.491452356883585	21.647128794071843
85-89	20.96015732461492	28.82915176710123	28.516539640550352	21.6941512677335
90-94	20.825042834318328	27.842238195282626	29.479982865215486	21.852736105183556
95-99	20.586153955296833	28.435976085528324	29.86451115929323	21.113358799881606
100-104	21.31280204385439	28.3674104643531	28.91028303940704	21.409504452385466
105-109	21.411994242556702	28.662265834057653	28.583174584949923	21.34256533843572
110-114	21.29234270558863	28.65487218040222	28.852093591785277	21.20069152222387
115-119	21.327766548958998	28.514217186243794	28.909506922978295	21.248509341818913
120-124	21.363837728922274	28.612699506496032	28.853969204998688	21.169493559583007
125-129	21.408904449063844	28.68620250413436	28.782887047773237	21.122005999028556
130-134	21.45693802982844	28.63691699584964	28.802122413530913	21.104022560791005
135-139	21.55684386385991	28.654344665241467	28.78914567252682	20.999665798371804
140-144	21.524266720234973	28.75298297555964	28.757463760799062	20.96528654340633
145-149	21.459740692899416	28.73676023571069	28.87825013151068	20.92524893987922
150-154	21.39998030948421	28.827862515134363	28.92547331813485	20.84668385724658
155-159	21.370049095299123	28.927592199395253	28.920027000911496	20.782331704394128
160-164	21.292414082946493	28.923088411769708	28.982517014954407	20.801980490329395
165-169	21.1949317664547	29.044926243784275	29.092223517797784	20.667918471963244
170-174	21.1836367497172	29.14417808591504	29.04288301280065	20.62930215156711
175-179	21.092599303105757	29.20110831431513	29.131829661472647	20.57446272110646
180-182	21.097040926938448	29.255538938056286	29.14401567558709	20.503404459418174
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	3.5
11	6.5
12	21.0
13	40.0
14	71.0
15	148.0
16	328.5
17	695.5
18	1423.0
19	2894.0
20	5640.5
21	9948.0
22	16288.0
23	25260.5
24	37093.5
25	51679.5
26	68373.0
27	86962.5
28	106943.0
29	127135.0
30	147055.0
31	166698.5
32	186177.0
33	205810.5
34	224702.5
35	242367.5
36	258944.5
37	274026.0
38	288314.0
39	300641.0
40	309952.5
41	315290.5
42	317386.0
43	315710.0
44	308556.5
45	298495.0
46	285079.0
47	268374.5
48	250144.5
49	231214.5
50	211616.5
51	192587.5
52	174393.0
53	157137.5
54	141705.0
55	127736.0
56	115196.5
57	103336.0
58	92497.5
59	82443.0
60	73033.5
61	64769.5
62	56943.5
63	49087.0
64	41564.5
65	34549.0
66	28405.0
67	23041.0
68	18135.0
69	13829.5
70	10405.0
71	7632.0
72	5326.5
73	3596.0
74	2365.5
75	1523.5
76	935.0
77	556.5
78	330.5
79	183.5
80	80.5
81	41.0
82	26.5
83	14.5
84	6.5
85	2.5
86	2.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-182	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1621.0
92-93	4252.0
94-95	6459.0
96-97	8832.0
98-99	11078.0
100-101	12318.0
102-103	14131.0
104-105	15352.0
106-107	17162.0
108-109	18298.0
110-111	19789.0
112-113	20355.0
114-115	21530.0
116-117	22072.0
118-119	22961.0
120-121	24113.0
122-123	25087.0
124-125	26426.0
126-127	26897.0
128-129	27599.0
130-131	28960.0
132-133	29846.0
134-135	79235.0
136-137	96665.0
138-139	116119.0
140-141	111635.0
142-143	105263.0
144-145	106243.0
146-147	95490.0
148-149	99256.0
150-151	106142.0
152-153	105197.0
154-155	104730.0
156-157	101277.0
158-159	88644.0
160-161	90013.0
162-163	92821.0
164-165	100747.0
166-167	100070.0
168-169	100744.0
170-171	105452.0
172-173	99078.0
174-175	109322.0
176-177	110901.0
178-179	115732.0
180-181	249603.0
182-183	4574055.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.03847653244407
#Duplication Level	Percentage of total
1	89.23830936646901
2	8.310258988841086
3	1.3704857668410557
4	0.3769947463605359
5	0.18051674570621423
6	0.10531004509494708
7	0.08625932683264768
8	0.05753638261244107
9	0.04527868021619823
>10	0.22762435542561182
>50	0.0014255956002851495
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.284314621751402E-5	2.642157310875701E-5	1.3210786554378504E-5	0.0	0.0	0.0
2	0.0017702453982867195	3.963235966313551E-5	1.3210786554378504E-5	0.0	0.0	0.0
3	0.001783456184841098	5.284314621751402E-5	1.3210786554378504E-5	1.3210786554378504E-5	0.0	0.0
4	0.001783456184841098	5.284314621751402E-5	2.642157310875701E-5	1.3210786554378504E-5	1.3210786554378504E-5	0.0
5	0.001783456184841098	5.284314621751402E-5	2.642157310875701E-5	2.642157310875701E-5	1.3210786554378504E-5	0.0
6	0.001783456184841098	5.284314621751402E-5	2.642157310875701E-5	2.642157310875701E-5	1.3210786554378504E-5	0.0
7	0.001783456184841098	6.605393277189252E-5	2.642157310875701E-5	3.963235966313551E-5	1.3210786554378504E-5	0.0
8	0.0017966669713954766	6.605393277189252E-5	3.963235966313551E-5	5.284314621751402E-5	1.3210786554378504E-5	0.0
9	0.0017966669713954766	6.605393277189252E-5	3.963235966313551E-5	6.605393277189252E-5	1.3210786554378504E-5	0.0
10-14	0.0018045934433281036	6.869609008276822E-5	5.548530352838972E-5	8.983334856977384E-5	1.8495101176129905E-5	0.0
15-19	0.0018098777579498549	7.926471932627103E-5	9.247550588064953E-5	1.2946570823290935E-4	3.170588773050841E-5	0.0
20-24	0.0018257307018151093	7.926471932627103E-5	1.1625492167853083E-4	2.0873042755918035E-4	6.341177546101682E-5	0.0
25-29	0.0018495101176129905	7.926471932627103E-5	1.3210786554378504E-4	3.012059334398299E-4	9.775982050240094E-5	0.0
30-34	0.0020714513317265496	7.926471932627103E-5	1.5060296671991495E-4	3.4876476503559244E-4	1.9023532638305047E-4	0.0
35-39	0.0028165396933934965	8.983334856977381E-5	1.7966669713954762E-4	4.095343831857336E-4	3.144167199942084E-4	0.0
40-44	0.003603902572034456	1.1889707898940652E-4	2.1665689949180747E-4	4.6766184402499895E-4	4.3331379898361495E-4	0.0
45-49	0.004214240910846743	1.5588728134166633E-4	2.9327946150720276E-4	5.310736194860159E-4	5.892010803252813E-4	0.0
50-54	0.004742672373021882	1.7174022520692055E-4	3.1177456268333267E-4	6.18264810744914E-4	6.948873727603093E-4	1.3210786554378504E-5
55-59	0.005170701857383746	1.7174022520692055E-4	3.672598662117224E-4	6.790344288950551E-4	7.926471932627101E-4	1.3210786554378504E-5
60-64	0.005667427431828378	1.7174022520692055E-4	4.1481869780748504E-4	7.265932604908177E-4	8.904070137651113E-4	1.3210786554378504E-5
65-69	0.0059818441518225875	1.7438238251779627E-4	4.3331379898361495E-4	7.530148335995748E-4	9.643874184696308E-4	2.1137258487005608E-5
70-74	0.009067883890925405	2.0344611293742894E-4	4.7030400133587477E-4	8.111422944388401E-4	0.0010568629243502803	3.170588773050841E-5
75-79	0.061353534915844644	2.3515200066793733E-4	5.442844060403943E-4	8.692697552781057E-4	0.0011255590144330484	3.963235966313551E-5
80-84	0.08286862189830549	2.642157310875701E-4	5.548530352838971E-4	8.904070137651111E-4	0.0012576668799768335	3.963235966313551E-5
85-89	0.08305621506737766	2.879951468854514E-4	5.812746083926542E-4	9.062599576303654E-4	0.0014346914198055053	3.963235966313551E-5
90-94	0.08317775430367794	3.329118211703383E-4	6.209069680557897E-4	9.326815307391224E-4	0.001463755150225138	3.963235966313551E-5
95-99	0.08335213668619573	3.5933339427909525E-4	6.341177546101682E-4	9.72313890402258E-4	0.001500745352577398	3.963235966313551E-5
100-104	0.08359257300148541	4.068922258748579E-4	6.394020692319196E-4	0.0010040197781327662	0.0015245247683752792	3.963235966313551E-5
105-109	0.08382244068753161	4.438824282271177E-4	6.499706984754224E-4	0.0010542207670394047	0.0015694414426601663	3.963235966313551E-5
110-114	0.08399946522736028	4.7558831595762615E-4	7.001716873820606E-4	0.001101779598635167	0.0015985051730797993	3.963235966313551E-5
115-119	0.0841923427110542	4.7558831595762615E-4	7.50372676288699E-4	0.0011519805875418055	0.0016170002742559287	3.963235966313551E-5
120-124	0.0844327790263439	4.86156945201129E-4	7.95289350573586E-4	0.001207465891070195	0.0016962649935821999	3.963235966313551E-5
125-129	0.08514087718565858	4.914412598228803E-4	8.243530809932186E-4	0.0012312453068680765	0.001764961083664968	3.963235966313551E-5
130-134	0.08624265678429374	5.12578518309886E-4	8.74554069899857E-4	0.001270877666531212	0.0018310150164368606	3.963235966313551E-5
135-139	0.0872255393039395	5.152206756207616E-4	8.956913283868625E-4	0.0012946570823290935	0.001891784634587002	3.963235966313551E-5
140-144	0.08828768654291154	5.205049902425131E-4	9.08902114941241E-4	0.0013369315993031047	0.0019736915112241486	3.963235966313551E-5
145-149	0.0892388631748268	5.284314621751402E-4	9.168285868738681E-4	0.001429407105183754	0.002002755241643781	3.963235966313551E-5
150-154	0.09008699567161789	5.284314621751402E-4	9.591031038478793E-4	0.0014611129929142624	0.0020925885902135554	3.963235966313551E-5
155-159	0.0908690742356371	5.310736194860159E-4	9.775982050240094E-4	0.0014848924087121438	0.0021533582083636963	3.963235966313551E-5
160-164	0.09181760871024147	5.416422487295187E-4	9.775982050240094E-4	0.001545662026862285	0.00220620135458121	3.963235966313551E-5
165-169	0.09284540790417213	5.416422487295187E-4	9.960933062001392E-4	0.001590578701147172	0.002248475871555221	3.963235966313551E-5
170-171	0.09332099622012975	5.416422487295187E-4	0.0010040197781327662	0.0016117159596341776	0.0022722552873531026	3.963235966313551E-5
>>END_MODULE
