##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293710
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8569687
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.037387946607616	41.0	40.0	41.0	38.0	41.0
2	40.139635788331596	41.0	40.0	41.0	38.0	41.0
3	40.153325436506606	41.0	40.0	41.0	38.0	41.0
4	40.15402907947513	41.0	40.0	41.0	38.0	41.0
5	40.14153504089472	41.0	40.0	41.0	38.0	41.0
6	40.12248883769034	41.0	40.0	41.0	38.0	41.0
7	40.12018338592763	41.0	40.0	41.0	38.0	41.0
8	40.096544832967645	41.0	40.0	41.0	38.0	41.0
9	40.06151648245729	41.0	40.0	41.0	38.0	41.0
10-14	39.98743330999137	41.0	40.0	41.0	38.0	41.0
15-19	39.82266225125842	41.0	40.0	41.0	38.0	41.0
20-24	39.60436120945841	40.0	39.2	41.0	37.0	41.0
25-29	39.502554877441845	40.0	39.0	41.0	36.4	41.0
30-34	39.25787660622844	40.0	38.6	41.0	35.4	41.0
35-39	38.8052960160622	40.0	37.4	41.0	35.0	41.0
40-44	38.32924418359737	39.2	36.2	41.0	35.0	41.0
45-49	37.563712607006536	37.8	35.0	40.4	34.6	41.0
50-54	36.78249261612471	36.4	35.0	39.2	34.0	41.0
55-59	35.80062842435201	35.2	34.6	37.4	33.4	39.6
60-64	35.44854177287922	35.0	34.8	36.2	33.6	38.8
65-69	35.269176738893734	35.0	35.0	36.0	34.0	37.0
70-74	35.07510582358492	35.0	35.0	35.0	34.0	36.4
75-79	34.985543042587196	35.0	35.0	35.0	33.2	36.0
80-84	34.849007764227565	35.0	34.8	35.0	32.8	36.0
85-89	40.06868145826097	41.0	40.0	41.0	38.0	41.0
90-94	40.00831651111169	41.0	40.0	41.0	38.0	41.0
95-99	39.85597748351078	41.0	40.0	41.0	37.8	41.0
100-104	39.682420509541465	41.0	39.6	41.0	37.0	41.0
105-109	39.42692464631909	40.0	39.0	41.0	36.0	41.0
110-114	39.20482695412797	40.0	38.2	41.0	35.0	41.0
115-119	38.61315129504346	39.4	37.4	40.4	34.8	40.6
120-124	38.573133902233124	40.0	36.8	40.8	35.0	41.0
125-129	38.008389277229185	38.8	35.4	41.0	34.8	41.0
130-134	37.24984372992992	37.2	35.0	39.8	34.0	41.0
135-139	36.578581742726804	36.0	35.0	39.0	34.0	41.0
140-144	35.99679897578035	35.2	35.0	37.0	34.0	39.2
145-149	35.42497195460278	35.0	35.0	36.0	34.0	37.8
150-154	35.034087068714896	35.0	35.0	35.4	34.0	36.6
155-159	34.810546886739715	35.0	35.0	35.0	34.0	36.0
160-164	34.69013311511848	35.0	35.0	35.0	33.6	36.0
165-168	34.44138424599011	35.0	34.75	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	3.0
30	179.0
31	2093.0
32	13289.0
33	55254.0
34	167434.0
35	452879.0
36	1273541.0
37	2916410.0
38	3100294.0
39	586031.0
40	2279.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.90654104403113	27.736345563146003	28.579503545462043	21.77760984736082
2	21.599050233689983	28.0681313098133	28.536701515469588	21.796116941027137
3	21.646519878730693	28.181869419501552	28.317241924938447	21.854368776829304
4	22.053839305916306	28.240296290868034	27.994838084518136	21.71102631869752
5	21.60438298388261	28.441493837522884	28.188870842073925	21.765252336520575
6	21.672716868189003	28.350942105586817	28.261697305864264	21.714643720359916
7	22.099593602426786	28.250308325146534	27.985444509233538	21.66465356319315
8	21.690780538425734	28.27692539995918	28.069438242026813	21.962855819588274
9	21.724504057149346	28.223784602634844	28.126254786201642	21.92545655401417
10-14	21.75888570959476	28.249293118873535	28.250565043974184	21.741256127557516
15-19	21.67473094408232	28.113430513856574	28.372387462926007	21.839451079135095
20-24	21.695441152051412	28.107908725254493	28.21391493061532	21.982735192078778
25-29	21.776288912302164	28.184863694555006	28.152799512981048	21.886047880161787
30-34	21.708967900461243	28.166153559634093	28.20143606178382	21.923442478120847
35-39	21.697326868530904	28.19060719487188	28.19974871894388	21.91231721765334
40-44	21.82904463138502	28.13063767673195	28.109292673116297	21.931025018766732
45-49	21.919589361898513	28.144988259197802	28.036116138197347	21.89930624070634
50-54	21.852562409805632	28.093616487976746	28.071744043860647	21.982077058356975
55-59	21.89457561285494	28.145581046308926	28.04823093305508	21.911612407781053
60-64	21.85784848384778	28.106023008775	28.079165551787366	21.95696295558986
65-69	21.79332570722828	28.136178135794225	28.10124337096559	21.969252786011907
70-74	21.814628702308497	28.143692996021908	28.08983805359519	21.951840248074404
75-79	21.81142205077035	28.14053068682672	28.06965295231903	21.978394310083903
80-84	21.758682668340164	28.129501112467704	28.096064652069558	22.015751567122578
85-89	21.706459057372808	28.186497359821892	28.319045958154597	21.7879976246507
90-94	21.75488481770564	28.306123178734616	28.147399221969486	21.791592781590253
95-99	21.63274943756637	28.281697491582324	28.322930673201004	21.7626223976503
100-104	21.68729110573565	28.11866809224042	28.33549326639533	21.858547535628606
105-109	21.75216770616287	28.17010528324577	28.145088670138545	21.93263834045281
110-114	21.672746264458056	28.20135099928963	28.182212590311202	21.943690145941115
115-119	21.709861853498236	28.198964681216637	28.185696193545205	21.905477271739922
120-124	21.68911222267896	28.231350738197904	28.179506068823212	21.900030970299923
125-129	21.74999654030338	28.179660244521877	28.095914259428717	21.974428955746024
130-134	21.871386665626144	28.218703949262615	28.05517046086008	21.854738924251162
135-139	21.80057788332406	28.226844797881377	28.099825660587786	21.87275165820678
140-144	21.73953707306778	28.276571721749335	28.169487310756953	21.814403894425933
145-149	21.74188740535973	28.286553421083855	28.19022395916872	21.781335214387695
150-154	21.694626458385834	28.386670198993762	28.180977575475495	21.737725767144905
155-159	21.596979038492243	28.39442574525085	28.25559634112261	21.752998875134296
160-164	21.612814654893256	28.489575626176833	28.265833900875524	21.631775818054386
165-168	21.542487103513803	28.508021738336986	28.290572656627944	21.658918501521267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	4.0
10	12.5
11	25.0
12	50.5
13	111.0
14	247.5
15	430.0
16	750.0
17	1324.0
18	2235.5
19	3721.5
20	5975.0
21	9266.5
22	13760.5
23	19705.5
24	27265.5
25	36554.0
26	48249.5
27	61698.0
28	76832.0
29	94247.0
30	113612.5
31	134715.0
32	157525.5
33	182324.0
34	207943.0
35	234246.0
36	261903.5
37	289816.5
38	316009.0
39	340863.5
40	363289.0
41	380166.0
42	391877.0
43	399131.5
44	399512.5
45	391574.0
46	377423.0
47	358167.5
48	334626.5
49	308232.5
50	279740.0
51	250568.0
52	222850.0
53	197263.5
54	173609.5
55	152216.5
56	133727.0
57	118368.0
58	104582.0
59	92118.5
60	81675.0
61	72151.0
62	63503.0
63	55182.0
64	47373.0
65	40357.0
66	33299.0
67	26809.5
68	21366.0
69	16738.5
70	12611.0
71	9269.5
72	6673.5
73	4584.5
74	3062.0
75	2014.5
76	1297.0
77	799.0
78	457.5
79	262.5
80	148.5
81	76.0
82	37.5
83	14.5
84	7.5
85	5.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	719.0
92-93	1751.0
94-95	2676.0
96-97	3787.0
98-99	4483.0
100-101	5010.0
102-103	5858.0
104-105	6649.0
106-107	7102.0
108-109	7641.0
110-111	8053.0
112-113	8474.0
114-115	8890.0
116-117	9455.0
118-119	9869.0
120-121	10324.0
122-123	11037.0
124-125	11460.0
126-127	12083.0
128-129	45578.0
130-131	79777.0
132-133	81573.0
134-135	82079.0
136-137	70271.0
138-139	67758.0
140-141	72987.0
142-143	74329.0
144-145	67378.0
146-147	65874.0
148-149	67255.0
150-151	67695.0
152-153	70782.0
154-155	71511.0
156-157	73654.0
158-159	77526.0
160-161	77243.0
162-163	81575.0
164-165	91750.0
166-167	187004.0
168-169	6860767.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.70377208566192
#Duplication Level	Percentage of total
1	89.90910636662733
2	8.797298849242269
3	0.992874258010394
4	0.17390601254245913
5	0.06444507855118194
6	0.028809316698688484
7	0.01345361499310764
8	0.0031227168908005485
9	0.006281644025087566
>10	0.010702142418688958
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.1669037620627218E-5	0.0	0.0
2	0.0	0.0	1.1669037620627218E-5	1.1669037620627218E-5	0.0	0.0
3	0.0	1.1669037620627218E-5	2.3338075241254436E-5	1.1669037620627218E-5	0.0	0.0
4	0.0	2.3338075241254436E-5	2.3338075241254436E-5	2.3338075241254436E-5	0.0	0.0
5	1.1669037620627218E-5	3.500711286188165E-5	3.500711286188165E-5	2.3338075241254436E-5	0.0	0.0
6	2.3338075241254436E-5	4.667615048250887E-5	3.500711286188165E-5	4.667615048250887E-5	0.0	0.0
7	2.3338075241254436E-5	5.8345188103136086E-5	3.500711286188165E-5	4.667615048250887E-5	0.0	0.0
8	2.3338075241254436E-5	5.8345188103136086E-5	3.500711286188165E-5	5.8345188103136086E-5	0.0	0.0
9	2.3338075241254436E-5	5.8345188103136086E-5	4.667615048250887E-5	8.168326334439052E-5	0.0	0.0
10-14	2.3338075241254436E-5	8.401707086851598E-5	4.667615048250887E-5	9.101849344089229E-5	2.3338075241254437E-6	0.0
15-19	2.3338075241254436E-5	1.1902418373039763E-4	6.768041819963785E-5	1.2135799125452305E-4	1.400284514475266E-5	0.0
20-24	4.667615048250887E-5	1.400284514475266E-4	7.00142257237633E-5	1.7270175678528286E-4	2.800569028950532E-5	0.0
25-29	4.667615048250887E-5	1.7503556430940826E-4	7.00142257237633E-5	2.357145599366698E-4	3.967472791013254E-5	0.0
30-34	1.1202276115802127E-4	2.0537506212303902E-4	8.401707086851598E-5	2.800569028950532E-4	6.301280315138698E-5	0.0
35-39	2.7772309537092775E-4	2.1004267717128993E-4	9.801991601326861E-5	3.0806259318455855E-4	9.101849344089229E-5	1.867046019300355E-5
40-44	4.597600822527124E-4	2.2871313736429346E-4	1.0968895363389585E-4	3.2439924585343663E-4	1.4702987401990294E-4	8.401707086851598E-5
45-49	5.57779998265981E-4	3.080625931845585E-4	1.3536083639927574E-4	3.5473874366706744E-4	1.9603983202653728E-4	1.0968895363389585E-4
50-54	6.884732196170059E-4	3.267330533775621E-4	1.5869891164053016E-4	3.944134715772E-4	2.4038217498492065E-4	1.330270288751503E-4
55-59	7.841593281061489E-4	3.384020909981893E-4	1.8903840945416094E-4	4.4575723710795975E-4	2.9405974803980593E-4	1.446960664957775E-4
60-64	8.285016710645324E-4	3.52404936142942E-4	2.3804836746079525E-4	4.900995800663432E-4	3.5707255119119284E-4	1.5169748906815382E-4
65-69	0.0017806951409077136	3.664077812876946E-4	2.613864427020497E-4	5.111038477834722E-4	4.177515468184544E-4	1.610327191646556E-4
70-74	0.021193306126583152	3.897458565289491E-4	2.800569028950532E-4	5.251066929282248E-4	4.480910446320852E-4	1.703679492611574E-4
75-79	0.029641689363917256	4.107501242460781E-4	2.917259405156804E-4	5.344419230247266E-4	5.694490358866082E-4	1.8670460193003549E-4
80-84	0.02969069932192389	4.3175439196320713E-4	2.9872736308805673E-4	5.367757305488521E-4	8.705102064987905E-4	1.8670460193003549E-4
85-89	0.029730374049834023	4.597600822527124E-4	3.0339497813630764E-4	5.531123832177301E-4	9.008497043124211E-4	1.8670460193003549E-4
90-94	0.029779384007840658	5.181052703558485E-4	3.0806259318455855E-4	5.834518810313608E-4	9.101849344089229E-4	1.8670460193003549E-4
95-99	0.029791053045461292	5.857856885554863E-4	3.5007112861881653E-4	6.044561487484898E-4	9.101849344089229E-4	1.8670460193003549E-4
100-104	0.029814391120702544	5.974547261761136E-4	3.780768189083218E-4	6.347956465621206E-4	9.218539720295502E-4	1.8670460193003549E-4
105-109	0.029844730618516176	6.417970691344969E-4	3.8741204900482365E-4	6.62801336851626E-4	9.335230096501774E-4	1.8670460193003549E-4
110-114	0.02989140676899868	6.674689518998769E-4	3.990810866254508E-4	7.071436798100095E-4	9.568610848914319E-4	1.8670460193003549E-4
115-119	0.029977757647391322	6.768041819963786E-4	4.1541773929432895E-4	7.468184077201419E-4	9.918681977533134E-4	1.8903840945416094E-4
120-124	0.03028115262552763	7.094774873341349E-4	4.2008535434257986E-4	8.168326334439053E-4	0.0010362105407116969	2.170440997436663E-4
125-129	0.03061955471652582	7.374831776236401E-4	4.387558145355834E-4	8.611749764022887E-4	0.0010922219212907078	2.26379329840168E-4
130-134	0.030967292037620508	7.538198302925182E-4	4.6909531234921414E-4	8.985158967882958E-4	0.0011388980717732164	2.403821749849207E-4
135-139	0.03113999379440579	7.771579055337726E-4	4.994348101628449E-4	9.428582397466792E-4	0.0011739051846350983	2.520512126055479E-4
140-144	0.0313243645888117	7.888269431543998E-4	5.274405004523504E-4	9.52193469843181E-4	0.0012509208329312376	2.567188276537988E-4
145-149	0.031553077726176	8.215002484921561E-4	5.671152283624828E-4	0.0010012034278498152	0.0012765927156966176	2.567188276537988E-4
150-154	0.03179345990116091	8.635087839264143E-4	5.881194960796118E-4	0.0010502133858564494	0.0013232688661791265	2.567188276537988E-4
155-157	0.03208985345672485	8.635087839264142E-4	5.951209186519881E-4	0.0010813308195114554	0.0013613877224065089	2.567188276537988E-4
>>END_MODULE
