##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293715
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5984934
Total Bases	986.8 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.65197611201727	39.0	37.0	39.0	35.0	39.0
2	39.317183781809455	40.0	39.0	41.0	36.0	41.0
3	39.39938418702696	40.0	39.0	41.0	36.0	41.0
4	39.43901720553644	40.0	39.0	41.0	37.0	41.0
5	39.460591211197986	40.0	39.0	41.0	37.0	41.0
6	39.44592521822296	40.0	39.0	41.0	37.0	41.0
7	39.471244962768175	40.0	39.0	41.0	37.0	41.0
8	39.45938685372303	40.0	39.0	41.0	37.0	41.0
9	39.44641979343465	40.0	39.0	41.0	37.0	41.0
10-14	39.38013842759169	40.0	39.0	41.0	36.2	41.0
15-19	39.21652913799885	40.0	38.6	41.0	36.0	41.0
20-24	39.01287803675027	40.0	38.0	41.0	35.4	41.0
25-29	38.74143561148711	40.0	38.0	41.0	35.0	41.0
30-34	38.50845352680581	40.0	37.4	40.8	34.4	41.0
35-39	37.98170760111974	39.2	36.0	40.0	33.6	41.0
40-44	37.56102757357057	38.6	35.0	40.0	33.2	41.0
45-49	36.79712758737189	36.8	35.0	39.8	33.0	41.0
50-54	36.025919717744586	35.6	34.2	38.8	33.0	40.2
55-59	35.36756378599998	35.0	34.0	37.0	32.0	39.4
60-64	34.784001862008836	34.8	33.8	35.8	31.8	38.8
65-69	34.60511404135785	35.0	33.8	35.0	32.0	37.4
70-74	34.55730980492015	35.0	34.0	35.0	32.0	37.0
75-79	34.521006246685424	35.0	33.0	35.0	32.0	37.4
80-84	34.53691385736251	35.0	33.0	35.0	32.0	38.0
85-89	34.44118862463647	34.8	33.0	35.0	31.6	38.4
90-94	38.19804233873987	39.8	37.6	40.6	32.8	40.6
95-99	38.4486381230263	40.0	37.6	41.0	33.0	41.0
100-104	38.314367412190485	40.0	37.0	41.0	33.0	41.0
105-109	38.13696835774442	40.0	36.6	41.0	33.0	41.0
110-114	37.9086888451031	40.0	36.0	41.0	32.4	41.0
115-119	37.63077116322172	39.2	35.0	40.4	32.0	41.0
120-124	37.2958963941673	38.8	35.0	40.0	32.0	41.0
125-129	36.7508663824106	37.6	34.6	39.8	31.6	40.6
130-134	36.41521982893097	36.8	34.0	40.0	31.0	41.0
135-139	35.97521500835774	35.8	34.0	39.2	31.0	41.0
140-144	35.55070447790208	35.0	34.0	38.0	31.0	40.2
145-149	34.9579308192634	35.0	34.0	36.4	31.0	39.2
150-154	34.46724976489353	35.0	34.0	35.4	31.0	37.2
155-159	34.14948975527654	35.0	33.2	35.0	31.0	36.2
160-164	33.957653590455145	35.0	33.0	35.0	31.0	35.8
165-169	33.854180872384134	35.0	33.0	35.0	31.0	35.0
170-174	33.8041034194285	35.0	33.0	35.0	31.0	35.0
175-178	33.57395754760219	34.75	32.75	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	4.0
28	135.0
29	1790.0
30	10407.0
31	43046.0
32	131427.0
33	265961.0
34	459660.0
35	832528.0
36	1447914.0
37	1814320.0
38	912080.0
39	65448.0
40	214.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.28898965301873	25.085322578327514	26.24952923457468	24.376158534079075
2	25.397305968620536	26.014956890084335	25.173961818125314	23.413775323169812
3	22.619113260062683	25.96489785852275	26.97052298321084	24.44546589820372
4	23.471152731174648	24.97695045592817	27.153733023622316	24.398163789274868
5	24.41211548865869	25.504692282320907	25.537858897023757	24.545333331996645
6	23.896637790826098	25.958815920108723	25.957061514796987	24.187484774268185
7	24.1088539990583	25.68845370725893	25.739565382007555	24.463126911675218
8	24.59069724077158	25.75610691780394	25.15038595245996	24.502809888964524
9	24.20588096710841	26.131766866602035	25.631794770000806	24.03055739628875
10-14	24.280909363411528	25.766873953831404	25.5592994007954	24.392917281961672
15-19	24.129068089973924	25.85906544666992	25.72640901303172	24.285457450324433
20-24	24.211491722381567	25.723688849367427	25.6617767213473	24.4030427069037
25-29	24.21050591368259	25.64770806160937	25.57094531034093	24.570840714367108
30-34	24.038300171731215	25.815980593938043	25.778559964069782	24.367159270260956
35-39	24.073037396903622	25.8080607071022	25.697710283856097	24.42119161213808
40-44	24.21423861984109	25.75715287754218	25.609241472002864	24.419367030613873
45-49	24.30715192515072	25.70901199578809	25.62634775922341	24.35748831983778
50-54	24.348098074264477	25.833394319803695	25.52011768216659	24.29838992376524
55-59	24.28933385063227	25.690037684626095	25.555997777084926	24.464630687656705
60-64	24.187224119764732	25.8604589457461	25.61041441726843	24.341902517220742
65-69	24.173817121458647	25.846707749826482	25.605515449293176	24.373959679421695
70-74	24.15807759951906	25.84544457800203	25.572124939055303	24.424352883423612
75-79	24.060840102831545	25.86786420702384	25.6863049784676	24.38499071167702
80-84	24.08811191568696	25.914715183158243	25.574751534436302	24.422421366718496
85-89	24.059971254486683	25.843703539587903	25.543155530203006	24.553169675722405
90-94	23.80201123649563	25.739178509667997	26.13466349005524	24.324146763781133
95-99	23.943395917123322	26.01866508288871	25.653679667320812	24.384259332667156
100-104	24.028207124772475	26.02594340456929	25.575709218311076	24.370140252347163
105-109	23.975511174980397	25.979442071584817	25.632074238298035	24.412972515136747
110-114	23.953156902453525	25.868448088467037	25.714780033430223	24.46361497564921
115-119	24.01635318297349	25.938673214095292	25.546848257508724	24.498125345422494
120-124	23.941935681587225	25.876097897155383	25.670119602993918	24.51184681826348
125-129	23.88145721167833	25.964194474382367	25.717302270333448	24.437046043605854
130-134	24.104771161857823	25.970887637491714	25.531773557250524	24.39256764339994
135-139	24.272744586555483	25.838235559707556	25.474238441564413	24.414781412172545
140-144	24.169553199568224	25.984657380138877	25.59537979905397	24.250409621238937
145-149	24.17241260963466	25.97856492774274	25.566535848688353	24.282486613934246
150-154	24.124809814247556	26.060989719330824	25.583990597621014	24.230209868800607
155-159	23.99070770066563	26.059971158238092	25.764152070772784	24.18516907032349
160-164	24.01800561274041	26.225033602109594	25.65628240638629	24.10067837876371
165-169	23.942335718372696	26.132571106107893	25.784606808002536	24.14048636751687
170-174	23.82628132594227	26.34748144809255	25.838464664837957	23.987772561127223
175-178	23.876969650510304	26.337981907073672	25.72887489228583	24.056173550130193
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	5.0
16	6.0
17	10.5
18	18.0
19	31.5
20	88.5
21	178.0
22	375.0
23	668.5
24	1085.0
25	1860.0
26	3061.0
27	4704.0
28	7072.5
29	10366.0
30	14882.0
31	20668.5
32	28161.0
33	37640.5
34	49764.0
35	65216.5
36	83470.0
37	104570.5
38	128479.0
39	153986.0
40	180027.5
41	206525.5
42	231333.0
43	253502.0
44	272349.5
45	286140.5
46	293596.0
47	296338.5
48	294345.5
49	285807.5
50	273073.5
51	256965.5
52	238638.5
53	220082.0
54	201351.0
55	182727.0
56	165612.0
57	150458.0
58	136764.5
59	123568.0
60	111264.5
61	100534.5
62	89754.0
63	79192.0
64	69337.5
65	59165.0
66	49453.5
67	40661.5
68	32473.0
69	25410.0
70	19288.5
71	14146.5
72	10278.5
73	7140.0
74	4830.5
75	3197.0
76	1987.5
77	1183.5
78	693.0
79	408.0
80	230.0
81	122.5
82	58.0
83	31.0
84	16.0
85	9.5
86	3.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2425.0
92-93	4658.0
94-95	6961.0
96-97	8915.0
98-99	11595.0
100-101	13215.0
102-103	15314.0
104-105	17073.0
106-107	17949.0
108-109	19610.0
110-111	21126.0
112-113	22160.0
114-115	23714.0
116-117	24514.0
118-119	25591.0
120-121	26342.0
122-123	27503.0
124-125	28392.0
126-127	29550.0
128-129	30591.0
130-131	31469.0
132-133	33139.0
134-135	129677.0
136-137	110167.0
138-139	100213.0
140-141	109294.0
142-143	109102.0
144-145	99158.0
146-147	97527.0
148-149	100196.0
150-151	100869.0
152-153	100237.0
154-155	102284.0
156-157	89352.0
158-159	95229.0
160-161	95580.0
162-163	96513.0
164-165	94251.0
166-167	98676.0
168-169	96290.0
170-171	113701.0
172-173	95328.0
174-175	103867.0
176-177	201526.0
178-179	3204091.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.3552499410863
#Duplication Level	Percentage of total
1	87.55963327018446
2	10.313305169036656
3	1.5519954563438105
4	0.34109081482078385
5	0.09296436814147487
6	0.04791478992891379
7	0.02205760323234234
8	0.01588718966739914
9	0.011747753652138412
>10	0.043403584992019156
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.341724403310045E-5	3.341724403310045E-5	1.6708622016550226E-5	0.0	0.0	0.0
2	6.850535026785592E-4	3.341724403310045E-5	3.341724403310045E-5	0.0	0.0	0.0
3	6.850535026785592E-4	5.0125866049650674E-5	3.341724403310045E-5	0.0	0.0	0.0
4	6.850535026785592E-4	5.0125866049650674E-5	3.341724403310045E-5	0.0	0.0	0.0
5	6.850535026785592E-4	5.0125866049650674E-5	5.0125866049650674E-5	0.0	1.6708622016550226E-5	0.0
6	6.850535026785592E-4	5.0125866049650674E-5	5.0125866049650674E-5	0.0	1.6708622016550226E-5	0.0
7	6.850535026785592E-4	6.68344880662009E-5	5.0125866049650674E-5	0.0	1.6708622016550226E-5	0.0
8	6.850535026785592E-4	6.68344880662009E-5	5.0125866049650674E-5	0.0	1.6708622016550226E-5	0.0
9	7.017621246951095E-4	6.68344880662009E-5	5.0125866049650674E-5	1.6708622016550226E-5	1.6708622016550226E-5	0.0
10-14	7.017621246951095E-4	7.685966127613103E-5	6.349276366289085E-5	3.6758968436410494E-5	1.6708622016550226E-5	0.0
15-19	7.151290223083495E-4	8.688483448606117E-5	6.68344880662009E-5	6.68344880662009E-5	1.6708622016550226E-5	0.0
20-24	7.351793687282098E-4	1.0025173209930135E-4	7.685966127613103E-5	8.354311008275112E-5	1.6708622016550226E-5	0.0
25-29	7.385210931315199E-4	1.0025173209930135E-4	9.691000769599131E-5	1.1361862971254152E-4	1.6708622016550226E-5	0.0
30-34	7.953304079877908E-4	1.1027690530923148E-4	1.4369414934233194E-4	1.336689761324018E-4	1.6708622016550226E-5	0.0
35-39	0.001052643187042664	1.269855273257817E-4	1.8379484218205246E-4	1.336689761324018E-4	1.6708622016550226E-5	1.0025173209930136E-5
40-44	0.001259830100047887	1.5706104695557211E-4	1.8379484218205246E-4	1.8045311777874245E-4	1.6708622016550226E-5	2.6733795226480362E-5
45-49	0.001353398383340568	1.7376966897212232E-4	2.2055381061846297E-4	2.038451886019127E-4	3.00755196297904E-5	4.678414164634063E-5
50-54	0.0015906608159755816	2.005034641986027E-4	2.506293302482534E-4	2.305789838283931E-4	3.341724403310045E-5	5.0125866049650674E-5
55-59	0.0017878225557708739	2.1052863740853283E-4	2.8070484987804376E-4	2.4394588144163327E-4	5.3467590452960723E-5	5.0125866049650674E-5
60-64	0.001951567051533066	2.5731277905487345E-4	3.141220939111442E-4	2.506293302482534E-4	8.354311008275112E-5	7.351793687282099E-5
65-69	0.002061843956842298	3.0075519629790403E-4	3.3751416473431454E-4	2.606545034581835E-4	8.354311008275112E-5	9.691000769599131E-5
70-74	0.0026433040030182453	3.1746381831445426E-4	3.5756451115417475E-4	2.673379522648036E-4	1.1027690530923148E-4	1.0025173209930135E-4
75-79	0.01346714934533948	3.441976135409346E-4	3.976652039938954E-4	2.7067967666811365E-4	1.336689761324018E-4	1.0025173209930135E-4
80-84	0.018579987682403848	3.675896843641049E-4	4.1437382601044564E-4	2.840465742813538E-4	1.6374449576219219E-4	1.1361862971254153E-4
85-89	0.01864682217047005	3.675896843641049E-4	4.444493456402359E-4	3.0075519629790403E-4	3.0075519629790403E-4	1.1696035411585157E-4
90-94	0.018713656658536252	3.9432347959058526E-4	4.544745188501661E-4	3.0075519629790403E-4	3.0075519629790403E-4	1.1696035411585157E-4
95-99	0.018793858044215694	4.444493456402359E-4	4.678414164634063E-4	3.1746381831445426E-4	3.0075519629790403E-4	1.1696035411585157E-4
100-104	0.018857350807878582	4.5113279444685604E-4	4.678414164634063E-4	3.3083071592769446E-4	3.0075519629790403E-4	1.1696035411585157E-4
105-109	0.018907476673928232	4.611579676567862E-4	4.812083140766464E-4	3.3751416473431454E-4	3.0075519629790403E-4	1.1696035411585157E-4
110-114	0.018974311161994432	4.678414164634063E-4	4.8455003847995657E-4	3.5422278675086477E-4	3.0075519629790403E-4	1.1696035411585157E-4
115-119	0.019091271516110286	4.678414164634063E-4	4.8455003847995657E-4	3.976652039938954E-4	3.0075519629790403E-4	1.1696035411585157E-4
120-124	0.01931182532672875	4.7452486527002635E-4	5.046003848998168E-4	4.010069283972054E-4	3.0075519629790403E-4	1.1696035411585157E-4
125-129	0.01961258052302665	4.979169360931967E-4	5.27992455722987E-4	4.076903772038255E-4	3.0075519629790403E-4	1.1696035411585157E-4
130-134	0.01996346158537421	5.179672825130569E-4	5.447010777395373E-4	4.5447451885016613E-4	3.0075519629790403E-4	1.1696035411585157E-4
135-139	0.020421277828627685	5.513845265461574E-4	6.015103925958081E-4	4.812083140766464E-4	3.0409692070121406E-4	1.1696035411585157E-4
140-144	0.020812259583814958	5.513845265461574E-4	6.015103925958081E-4	5.012586604965067E-4	3.1746381831445426E-4	1.1696035411585157E-4
145-149	0.02106288891406321	5.714348729660176E-4	6.015103925958081E-4	5.179672825130569E-4	3.1746381831445426E-4	1.269855273257817E-4
150-154	0.02118319099258237	5.848017705792579E-4	6.081938414024281E-4	5.346759045296072E-4	3.1746381831445426E-4	1.336689761324018E-4
155-159	0.021330226866328017	5.848017705792579E-4	6.182190146123583E-4	5.781183217726377E-4	3.1746381831445426E-4	1.336689761324018E-4
160-164	0.02156414757455972	5.848017705792579E-4	6.182190146123583E-4	5.848017705792579E-4	3.1746381831445426E-4	1.336689761324018E-4
165-167	0.02173234770285966	5.848017705792579E-4	6.182190146123583E-4	5.959408519236247E-4	3.1746381831445426E-4	1.336689761324018E-4
>>END_MODULE
