##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293716
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5776457
Total Bases	951.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.637860197003114	39.0	37.0	39.0	35.0	39.0
2	39.28647456390656	40.0	39.0	41.0	36.0	41.0
3	39.370963204607946	40.0	39.0	41.0	36.0	41.0
4	39.411132117836246	40.0	39.0	41.0	37.0	41.0
5	39.42825870598535	40.0	39.0	41.0	37.0	41.0
6	39.41019815433578	40.0	39.0	41.0	36.0	41.0
7	39.44992596673013	40.0	39.0	41.0	37.0	41.0
8	39.41735098175231	40.0	39.0	41.0	37.0	41.0
9	39.404650289961474	40.0	39.0	41.0	37.0	41.0
10-14	39.348224456617615	40.0	39.0	41.0	36.0	41.0
15-19	39.19611616601664	40.0	38.4	41.0	36.0	41.0
20-24	38.98373913282831	40.0	38.0	41.0	35.2	41.0
25-29	38.71970957284024	40.0	38.0	41.0	35.0	41.0
30-34	38.49298104357048	40.0	37.4	40.8	34.4	41.0
35-39	37.97751313651257	39.2	36.0	40.0	33.6	41.0
40-44	37.566508432418004	38.6	35.0	40.0	33.2	41.0
45-49	36.80610606120673	36.8	35.0	39.8	33.0	41.0
50-54	36.03526344955047	35.6	34.2	38.8	33.0	40.2
55-59	35.37006511084563	35.0	34.0	37.0	32.0	39.4
60-64	34.78530427907626	34.8	33.8	35.8	31.8	38.8
65-69	34.597680031202515	35.0	33.6	35.0	32.0	37.4
70-74	34.55324192666889	35.0	34.0	35.0	32.0	37.0
75-79	34.51795538337773	35.0	33.0	35.0	32.0	37.4
80-84	34.531624765838295	35.0	33.0	35.0	32.0	38.0
85-89	34.42286938169885	34.8	33.0	35.0	31.6	38.4
90-94	38.176029478342265	39.8	37.6	40.6	32.8	40.6
95-99	38.43135933695699	40.0	37.6	41.0	33.0	41.0
100-104	38.29086781274964	40.0	37.0	41.0	33.0	41.0
105-109	38.12906232954191	40.0	36.6	41.0	33.0	41.0
110-114	37.8922778467091	40.0	36.0	41.0	32.4	41.0
115-119	37.61795840347155	39.2	35.0	40.2	32.0	41.0
120-124	37.28267229444718	38.6	35.0	40.0	32.0	41.0
125-129	36.734522190696566	37.6	34.6	39.6	31.6	40.6
130-134	36.41565653839639	36.8	34.0	40.0	31.0	41.0
135-139	35.96123017064015	35.8	34.0	39.2	31.0	41.0
140-144	35.560644548335745	35.0	34.0	38.0	31.0	40.2
145-149	34.96055687949702	35.0	34.0	36.6	31.0	39.2
150-154	34.47858240585955	35.0	34.0	35.4	31.0	37.2
155-159	34.15789914129398	35.0	33.0	35.0	31.0	36.2
160-164	33.96244314783452	35.0	33.0	35.0	31.0	35.8
165-169	33.86168079209011	35.0	33.0	35.0	31.0	35.0
170-174	33.822403113423434	35.0	33.0	35.0	31.0	35.0
175-178	33.597281193225626	34.75	32.75	35.0	30.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	3.0
28	149.0
29	1586.0
30	9415.0
31	37774.0
32	120097.0
33	256824.0
34	454756.0
35	825043.0
36	1409684.0
37	1736570.0
38	863149.0
39	61206.0
40	201.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.219846179067897	25.176678368764797	26.299840888627752	24.303634563539553
2	25.320642047538826	26.075204922325224	25.25047793136866	23.35367509876729
3	22.617410637697123	26.006235309983268	27.00700446657873	24.369349585740878
4	23.399325918984594	25.018155592606334	27.25592867738823	24.32658981102084
5	24.330294504053263	25.569220025354646	25.637220185314284	24.46326528527781
6	23.81497862790288	25.979696551017344	26.046207909104147	24.159116911975627
7	24.044790777460996	25.705428085070135	25.776215420628944	24.473565716839925
8	24.57011278712886	25.78059526799905	25.2122364972162	24.43705544765589
9	24.15392341707036	26.13837513202297	25.705531954968247	24.00216949593843
10-14	24.248386164737312	25.804564978151834	25.603254728633836	24.343794128477022
15-19	24.069594909128554	25.913174805940738	25.76680134553066	24.250428939400052
20-24	24.139450877934348	25.778524794696818	25.726652167583	24.355372159785833
25-29	24.15306822157596	25.69732969534786	25.62461037968429	24.524991703391887
30-34	24.001601673828784	25.85565165636999	25.821464610573575	24.321282059227656
35-39	24.032399098617024	25.86405473112671	25.74656056471986	24.356985605536405
40-44	24.156056212311455	25.830532452678174	25.655781736105716	24.357629598904655
45-49	24.255317749270876	25.74118356632794	25.691395261836103	24.312103422565077
50-54	24.283944293188714	25.896576396223498	25.55926928911615	24.26021002147164
55-59	24.257744842556605	25.733528354837574	25.603275502613453	24.405451299992365
60-64	24.14497675651355	25.89448168661171	25.666231047855113	24.294310509019628
65-69	24.12223963581829	25.883506100711905	25.673941656624464	24.320312606845338
70-74	24.105506195233513	25.88975560624791	25.62054560433844	24.384192594180135
75-79	23.987129134692772	25.912098021330376	25.750680737344705	24.350092106632147
80-84	24.03307425295471	25.9798523558645	25.605661047939936	24.38141234324085
85-89	24.000140570595434	25.89452669690089	25.606215020729834	24.49911771177384
90-94	23.74403490102508	25.77541090594681	26.167396196192865	24.31315799683524
95-99	23.87845710081324	26.06885180447677	25.709137862392613	24.343553232317376
100-104	23.942621364924374	26.07300150656848	25.654430740616274	24.329946387890867
105-109	23.91111425795817	26.026840785120687	25.690510958861683	24.371533998059462
110-114	23.896491406574192	25.922734263561516	25.763474396547952	24.417299933316336
115-119	23.94644585494769	25.985944610836253	25.606237888550627	24.461371645665427
120-124	23.88491684799897	25.9501087993809	25.69847535945823	24.466498993161906
125-129	23.826330925828664	26.024080427814393	25.759270201566682	24.390318444790264
130-134	24.041067059584336	26.021451137995637	25.598374378045502	24.33910742437452
135-139	24.202423273751577	25.897597855646694	25.527336018703483	24.372642851898245
140-144	24.106702790945146	26.04145479304521	25.66211402066226	24.189728395347387
145-149	24.11663809805017	26.02876966948494	25.622543306861118	24.232048925603767
150-154	24.08566611679763	26.12135390312472	25.621112248162632	24.17186773191502
155-159	23.935280104614094	26.13040591687478	25.81204002275721	24.12227395575392
160-164	23.96840120448955	26.30529544364311	25.69915336699729	24.027149984870043
165-169	23.874139751771164	26.213397715740417	25.810876156737383	24.101586375751033
170-174	23.778929986927473	26.41127050085743	25.893533993074406	23.91626551914069
175-178	23.818881090472736	26.412120946976327	25.74826254343641	24.020735419114523
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	4.5
17	9.5
18	19.0
19	41.0
20	76.0
21	164.5
22	339.5
23	620.5
24	1086.5
25	1873.5
26	3001.0
27	4578.0
28	6904.5
29	10116.5
30	14480.0
31	20198.5
32	27568.5
33	37145.5
34	49053.0
35	63757.0
36	81311.0
37	101899.5
38	125580.0
39	150851.0
40	176591.5
41	201935.0
42	225711.0
43	247395.0
44	264935.0
45	277519.5
46	285678.0
47	288047.0
48	284359.0
49	276071.0
50	264117.5
51	248269.0
52	230298.5
53	211283.0
54	192194.0
55	174608.0
56	158291.0
57	143250.0
58	129624.5
59	117491.5
60	106094.0
61	95111.0
62	84897.5
63	74824.0
64	65011.0
65	55586.0
66	46449.5
67	37925.0
68	30369.0
69	23885.0
70	18128.0
71	13383.0
72	9533.5
73	6570.0
74	4538.5
75	2951.5
76	1783.5
77	1088.5
78	674.5
79	390.5
80	193.0
81	92.5
82	49.5
83	21.0
84	14.0
85	10.0
86	4.0
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	2226.0
92-93	4795.0
94-95	7215.0
96-97	9016.0
98-99	11062.0
100-101	12875.0
102-103	14823.0
104-105	16338.0
106-107	17865.0
108-109	19766.0
110-111	20838.0
112-113	21578.0
114-115	23171.0
116-117	23735.0
118-119	24799.0
120-121	25926.0
122-123	26589.0
124-125	28226.0
126-127	28943.0
128-129	29369.0
130-131	31131.0
132-133	33082.0
134-135	122362.0
136-137	109214.0
138-139	99849.0
140-141	102579.0
142-143	100582.0
144-145	101967.0
146-147	98402.0
148-149	98759.0
150-151	103283.0
152-153	97630.0
154-155	93082.0
156-157	92584.0
158-159	88392.0
160-161	92910.0
162-163	94856.0
164-165	97251.0
166-167	96951.0
168-169	95808.0
170-171	99419.0
172-173	97088.0
174-175	107804.0
176-177	207362.0
178-179	3044955.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.76741280932309
#Duplication Level	Percentage of total
1	88.32724329412025
2	9.701163658698036
3	1.4269378468465812
4	0.30141812679032415
5	0.09721264508807836
6	0.05111550338128255
7	0.028409969573947275
8	0.02228771423863591
9	0.01284563286748407
>10	0.031365608395420734
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.7311649684226855E-5	0.0	0.0	0.0	0.0	0.0
2	4.6741454147412507E-4	0.0	0.0	1.7311649684226855E-5	0.0	0.0
3	4.847261911583519E-4	1.7311649684226855E-5	0.0	3.462329936845371E-5	0.0	0.0
4	4.847261911583519E-4	1.7311649684226855E-5	0.0	3.462329936845371E-5	0.0	0.0
5	4.847261911583519E-4	1.7311649684226855E-5	0.0	3.462329936845371E-5	0.0	0.0
6	4.847261911583519E-4	1.7311649684226855E-5	1.7311649684226855E-5	3.462329936845371E-5	0.0	0.0
7	4.847261911583519E-4	1.7311649684226855E-5	1.7311649684226855E-5	3.462329936845371E-5	0.0	0.0
8	4.847261911583519E-4	1.7311649684226855E-5	1.7311649684226855E-5	3.462329936845371E-5	0.0	0.0
9	4.847261911583519E-4	3.462329936845371E-5	3.462329936845371E-5	3.462329936845371E-5	0.0	0.0
10-14	5.193494905268056E-4	3.462329936845371E-5	3.462329936845371E-5	5.193494905268056E-5	3.462329936845371E-6	0.0
15-19	5.366611402110325E-4	3.462329936845371E-5	4.154795924214445E-5	8.655824842113426E-5	1.7311649684226855E-5	0.0
20-24	5.574351198321047E-4	3.462329936845371E-5	6.578426880006203E-5	1.3849319747381484E-4	1.7311649684226855E-5	0.0
25-29	5.712844395794862E-4	4.847261911583519E-5	7.617125861059817E-5	1.7311649684226854E-4	1.7311649684226855E-5	0.0
30-34	6.059077389479399E-4	5.193494905268056E-5	8.655824842113427E-5	1.8350348665280462E-4	1.7311649684226855E-5	0.0
35-39	9.14055103327178E-4	5.193494905268056E-5	8.655824842113427E-5	2.2505144589494912E-4	1.7311649684226855E-5	0.0
40-44	0.0011945038282116529	7.617125861059816E-5	1.177192178527426E-4	2.735240650107843E-4	3.462329936845371E-5	0.0
45-49	0.001409168284296066	1.0386989810536112E-4	1.3849319747381484E-4	2.908357146950111E-4	7.270892867375279E-5	0.0
50-54	0.0016515313798752419	1.0386989810536112E-4	1.3849319747381484E-4	3.0468503444239264E-4	8.655824842113427E-5	1.7311649684226855E-5
55-59	0.0018523465162122734	1.1079455797905188E-4	1.5234251722119632E-4	3.496953236213825E-4	1.0733222804220649E-4	1.7311649684226855E-5
60-64	0.001904281465264954	1.9389047646334076E-4	1.904281465264954E-4	3.6354464336876396E-4	1.4541785734750555E-4	3.116096943160834E-5
65-69	0.001970065734065016	2.3890076564233058E-4	2.1812678602125835E-4	3.7393163317930004E-4	1.558048471580417E-4	3.462329936845371E-5
70-74	0.002607134442444564	2.4236309557917595E-4	2.2505144589494907E-4	3.947056128003723E-4	1.558048471580417E-4	3.462329936845371E-5
75-79	0.014780686500392886	2.596747452634028E-4	2.3197610576863983E-4	4.154795924214445E-4	1.8350348665280468E-4	3.462329936845371E-5
80-84	0.02031348973947179	2.596747452634028E-4	2.6313707520024814E-4	4.224042522951352E-4	2.5967474526340285E-4	3.462329936845371E-5
85-89	0.020393123328019232	2.8737338475816576E-4	2.839110548213204E-4	4.570275516635889E-4	3.9816794273721766E-4	3.462329936845371E-5
90-94	0.020448520607008757	3.185343541897741E-4	2.942980446318565E-4	4.951131809688879E-4	3.9816794273721766E-4	3.462329936845371E-5
95-99	0.020503917885998285	3.808562930529908E-4	2.942980446318565E-4	5.124248306531148E-4	3.9816794273721766E-4	3.462329936845371E-5
100-104	0.020562777494924658	4.120172624845991E-4	3.2545901406346486E-4	5.22811820463651E-4	3.9816794273721766E-4	3.462329936845371E-5
105-109	0.020645873413408945	4.3279124210567136E-4	3.462329936845371E-4	5.366611402110325E-4	3.9816794273721766E-4	3.462329936845371E-5
110-114	0.020753205641451153	4.3279124210567136E-4	3.531576535582278E-4	5.50510459958414E-4	3.9816794273721766E-4	3.462329936845371E-5
115-119	0.020891698838924964	4.3279124210567136E-4	3.808562930529908E-4	5.6089744976895E-4	3.9816794273721766E-4	3.462329936845371E-5
120-124	0.021064815335767234	4.6395221153727967E-4	3.9816794273721766E-4	5.851337593268677E-4	3.9816794273721766E-4	3.462329936845371E-5
125-129	0.021379887360020163	4.7433920134781575E-4	4.258665822319806E-4	6.232193886321668E-4	4.154795924214445E-4	3.462329936845371E-5
130-134	0.021715733363894166	4.985755109057333E-4	4.431782319162075E-4	6.266817185690122E-4	4.258665822319806E-4	3.462329936845371E-5
135-139	0.022193534895178824	5.158871605899602E-4	4.708768714109704E-4	6.578426880006204E-4	4.3279124210567136E-4	4.501028917898982E-5
140-144	0.022539767888863364	5.297364803373417E-4	5.020378408425787E-4	6.682296778111566E-4	4.3279124210567136E-4	5.193494905268056E-5
145-149	0.022754432344947777	5.539727898952594E-4	5.020378408425787E-4	6.99390647242765E-4	4.3279124210567136E-4	5.193494905268056E-5
150-154	0.02293447350166374	5.539727898952594E-4	5.055001707794241E-4	7.09777637053301E-4	4.3279124210567136E-4	5.193494905268056E-5
155-159	0.023235696206169283	5.816714293900222E-4	5.193494905268056E-4	7.09777637053301E-4	4.3279124210567136E-4	6.924659873690742E-5
160-164	0.023623477159095966	5.920584192005584E-4	5.262741504004963E-4	7.09777637053301E-4	4.3279124210567136E-4	6.924659873690742E-5
165-167	0.023901617664022542	6.059077389479399E-4	5.366611402110325E-4	7.09777637053301E-4	4.3279124210567136E-4	9.232879831587655E-5
>>END_MODULE
