##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293720
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4862033
Total Bases	802.3 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.6274679748163	39.0	37.0	39.0	35.0	39.0
2	39.274853749450074	40.0	39.0	41.0	36.0	41.0
3	39.37491251087765	40.0	39.0	41.0	36.0	41.0
4	39.42906928027843	40.0	39.0	41.0	37.0	41.0
5	39.44964441829169	40.0	39.0	41.0	37.0	41.0
6	39.441415556003015	40.0	39.0	41.0	37.0	41.0
7	39.47953088759373	40.0	39.0	41.0	37.0	41.0
8	39.46015504213978	40.0	39.0	41.0	37.0	41.0
9	39.45294612356601	40.0	39.0	41.0	37.0	41.0
10-14	39.387473264784504	40.0	39.0	41.0	36.4	41.0
15-19	39.24014917216728	40.0	38.8	41.0	36.0	41.0
20-24	39.06604504741124	40.0	38.0	41.0	35.6	41.0
25-29	38.86017606215342	40.0	38.0	41.0	35.0	41.0
30-34	38.70029767383315	40.0	37.8	41.0	35.0	41.0
35-39	38.286329854198854	39.8	36.8	40.2	34.0	41.0
40-44	37.984312035726624	39.0	35.8	40.0	34.0	41.0
45-49	37.35052036874286	38.0	35.0	40.0	33.0	41.0
50-54	36.63691908302556	36.6	35.0	39.6	33.0	41.0
55-59	35.92688256126604	35.4	34.0	38.4	32.8	40.0
60-64	35.19839367606102	35.0	33.8	36.8	31.8	39.4
65-69	34.8301605933156	35.0	33.8	36.0	32.0	38.6
70-74	34.618995428455534	35.0	34.0	35.4	32.0	37.0
75-79	34.48027773567148	35.0	33.4	35.0	32.0	37.0
80-84	34.42083675696977	35.0	33.0	35.0	32.0	37.0
85-89	34.278327234718475	34.8	33.0	35.0	31.6	37.2
90-94	37.56374244224863	39.8	35.6	40.6	31.2	40.6
95-99	37.912258659681115	40.0	36.0	41.0	32.0	41.0
100-104	37.80883487132447	40.0	36.0	41.0	31.4	41.0
105-109	37.66208718311983	40.0	35.4	41.0	31.0	41.0
110-114	37.46673341820509	39.4	35.0	40.2	31.0	41.0
115-119	37.2417137746746	39.0	35.0	40.0	31.0	41.0
120-124	36.98786655255119	38.4	34.0	40.0	30.0	41.0
125-129	36.56926188910827	37.6	33.8	39.8	30.0	40.6
130-134	36.36296222672277	37.2	33.8	40.0	30.0	41.0
135-139	36.085415500738335	36.2	33.4	40.0	30.0	41.0
140-144	35.823460056279565	35.6	34.0	39.2	30.0	41.0
145-149	35.297045839679335	35.0	33.0	38.0	30.0	40.0
150-154	34.77494822990343	35.0	33.0	36.6	30.0	39.2
155-159	34.32545691347231	35.0	33.0	35.8	30.0	37.6
160-164	33.99021907791207	35.0	33.0	35.0	30.0	36.4
165-169	33.77712968546051	35.0	33.0	35.0	30.0	36.0
170-174	33.683537691788004	35.0	33.0	35.0	30.0	35.4
175-178	33.452068523367245	34.75	32.75	35.0	29.75	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	3.0
28	135.0
29	1817.0
30	10480.0
31	44223.0
32	141758.0
33	290329.0
34	421797.0
35	622550.0
36	978037.0
37	1263165.0
38	918756.0
39	167915.0
40	1068.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.212690247063318	27.81311850413191	29.335382956059743	21.638808292745033
2	21.676035518475505	28.54412958529899	29.24813550216545	20.53169939406006
3	19.462517017058502	26.51703515792674	31.357561744233326	22.662886080781433
4	20.679518217996463	25.54009814413024	31.670352710481396	22.110030927391897
5	21.914989059103466	26.62421254648004	29.032546673377162	22.428251721039327
6	22.37093002042561	26.841878695599146	28.227122275805204	22.56006900817004
7	22.37407685221388	27.423939738788285	27.765874892251862	22.43610851674598
8	22.41381331636375	27.570627348683153	27.55596270119927	22.459596633753822
9	22.811239660446567	27.43189937213507	27.129104224508556	22.627756742909806
10-14	22.598081913471177	27.673839317832684	27.219301061922042	22.5087777067741
15-19	22.5921255573543	27.55611490090668	27.37142261272188	22.48033692901714
20-24	22.400464990673655	27.389752393700327	27.640746165235818	22.569036450390197
25-29	22.4380418643806	27.46532160518038	27.426465431230106	22.67017109920891
30-34	22.503133977083248	27.541939760589862	27.37646165708871	22.57846460523818
35-39	22.43118876404171	27.5184393030652	27.40100694503719	22.649364987855904
40-44	22.48574207538287	27.559019035864218	27.361607788346976	22.593631100405943
45-49	22.759224382064048	27.40741990027628	27.229189929397847	22.604165788261824
50-54	22.649986127202347	27.482668258319105	27.275409278382106	22.591936336096445
55-59	22.567892895831847	27.496691198928513	27.304730346338662	22.63068555890098
60-64	22.590632354819476	27.52970208141327	27.27745780417369	22.602207759593572
65-69	22.50645357610695	27.491249031012337	27.34431461078113	22.657982782099587
70-74	22.433356581495847	27.60122771688304	27.31728887895249	22.648126822668623
75-79	22.465544762859487	27.52181237766177	27.335240217415226	22.677402642063516
80-84	22.437490654629453	27.579146418792305	27.310727837511596	22.672635089066652
85-89	22.40791454932535	27.60223552575641	27.284997859948707	22.70485206496953
90-94	20.956669412783985	27.293332037847446	29.609152978158676	22.14084557120989
95-99	22.011634001692478	27.66059970732663	27.747480153545855	22.580286137435042
100-104	22.185490407365283	27.751592363813693	27.444831250933067	22.618085977887958
105-109	22.06846354414105	27.73664039660211	27.610909476447475	22.583986582809366
110-114	22.10167293836481	27.529315076781387	27.69269295345722	22.676319031396584
115-119	22.13581876181069	27.589008133464393	27.522609477766924	22.752563626957997
120-124	22.100504039700986	27.64664098302046	27.54270838383352	22.710146593445035
125-129	22.128372584811984	27.634556082804167	27.53572006389805	22.701351268485794
130-134	22.292248706527747	27.606901406400624	27.444308748402175	22.65654113866945
135-139	22.42149693917488	27.594150966176084	27.35390181796562	22.630450276683415
140-144	22.401466202556335	27.685821264070952	27.413335740600438	22.499376792772278
145-149	22.33953422085193	27.680030100205688	27.481755965873568	22.498679713068814
150-154	22.22190939272132	27.842929755104567	27.537427522623446	22.397733329550668
155-159	22.222957460299703	27.833993171609002	27.565140482962736	22.377908885128566
160-164	22.120679563963066	27.968935825246433	27.63914154067525	22.271243070115247
165-169	21.97723969697835	28.05465320740428	27.740859298955417	22.227247796661956
170-174	22.012742270898343	28.17033017478557	27.743364780013742	22.073562774302346
175-178	21.879913760739765	28.238645549887824	27.82174132998156	22.05969935939085
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	0.0
10	0.5
11	1.0
12	7.5
13	17.0
14	37.5
15	78.0
16	154.5
17	337.0
18	691.5
19	1324.5
20	2365.5
21	4037.5
22	6425.5
23	9779.5
24	14285.5
25	19879.0
26	26604.0
27	34473.5
28	43544.5
29	53503.0
30	64172.5
31	75495.5
32	87264.0
33	99828.0
34	113194.0
35	126004.0
36	139098.5
37	152231.0
38	164159.5
39	175628.5
40	185575.0
41	193615.5
42	199876.5
43	202747.0
44	202510.0
45	200014.0
46	194520.0
47	186099.0
48	176317.0
49	165031.5
50	153091.5
51	141221.0
52	129618.5
53	118287.0
54	107672.0
55	98427.0
56	90310.5
57	83533.5
58	77040.0
59	71113.5
60	66539.5
61	61650.5
62	56116.5
63	50891.5
64	45103.5
65	39334.0
66	33920.0
67	28496.0
68	23245.0
69	18453.5
70	14176.0
71	10605.5
72	7725.5
73	5443.5
74	3713.0
75	2478.5
76	1596.0
77	1010.5
78	626.5
79	341.5
80	188.5
81	113.0
82	61.5
83	33.0
84	12.5
85	3.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1620.0
92-93	3468.0
94-95	5094.0
96-97	6734.0
98-99	8295.0
100-101	9648.0
102-103	11018.0
104-105	12129.0
106-107	13666.0
108-109	14553.0
110-111	15577.0
112-113	16417.0
114-115	16727.0
116-117	18033.0
118-119	19282.0
120-121	19767.0
122-123	20027.0
124-125	20772.0
126-127	21571.0
128-129	22272.0
130-131	23145.0
132-133	24924.0
134-135	103151.0
136-137	98316.0
138-139	83838.0
140-141	88849.0
142-143	94841.0
144-145	85978.0
146-147	88473.0
148-149	94977.0
150-151	87140.0
152-153	74409.0
154-155	79921.0
156-157	78930.0
158-159	75320.0
160-161	96679.0
162-163	72711.0
164-165	70560.0
166-167	77176.0
168-169	78485.0
170-171	78078.0
172-173	76508.0
174-175	80017.0
176-177	158509.0
178-179	2614428.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.98526951942556
#Duplication Level	Percentage of total
1	90.41122541909972
2	8.44813720174922
3	0.893240191693573
4	0.1442304783039409
5	0.04371469812288243
6	0.02208162363083385
7	0.011684825816751477
8	0.0064384747286589
9	0.0021679450559802717
>10	0.013702346966544076
>50	0.0
>100	0.00337679483188873
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.4681033633461557E-4	0.0	0.0	0.0	0.0	0.0
2	7.609985370317314E-4	0.0	0.0	2.0567528027884632E-5	0.0	0.0
3	7.81566065059616E-4	0.0	0.0	2.0567528027884632E-5	2.0567528027884632E-5	0.0
4	7.81566065059616E-4	0.0	0.0	2.0567528027884632E-5	2.0567528027884632E-5	0.0
5	7.81566065059616E-4	0.0	0.0	2.0567528027884632E-5	2.0567528027884632E-5	0.0
6	7.81566065059616E-4	0.0	0.0	2.0567528027884632E-5	4.1135056055769264E-5	0.0
7	7.81566065059616E-4	2.0567528027884632E-5	0.0	2.0567528027884632E-5	4.1135056055769264E-5	0.0
8	7.81566065059616E-4	4.1135056055769264E-5	0.0	2.0567528027884632E-5	4.1135056055769264E-5	0.0
9	7.81566065059616E-4	4.1135056055769264E-5	0.0	2.0567528027884632E-5	6.170258408365389E-5	0.0
10-14	8.144741099042315E-4	4.1135056055769264E-5	2.0567528027884632E-5	5.758907847807697E-5	8.227011211153853E-5	0.0
15-19	8.268146267209623E-4	4.1135056055769264E-5	2.0567528027884632E-5	1.0695114574500008E-4	9.461062892826932E-5	0.0
20-24	8.4326864914327E-4	4.1135056055769264E-5	2.0567528027884632E-5	1.3163217937846165E-4	1.0283764013942315E-4	0.0
25-29	8.844037051990392E-4	4.1135056055769264E-5	3.7021550450192334E-5	1.4808620180076936E-4	1.0283764013942315E-4	0.0
30-34	0.001053057435027693	4.524856166134619E-5	8.227011211153853E-5	1.6454022422307706E-4	1.3574568498403856E-4	0.0
35-39	0.0017523533879757705	6.170258408365389E-5	8.227011211153853E-5	1.8099424664538478E-4	2.180157970955771E-4	2.468103363346156E-5
40-44	0.002632643587569233	6.170258408365389E-5	9.049712332269239E-5	2.056752802788463E-4	3.085129204182695E-4	6.170258408365389E-5
45-49	0.0033319395405173105	9.46106289282693E-5	1.0695114574500008E-4	2.056752802788463E-4	3.90783032529808E-4	6.992959529480775E-5
50-54	0.004084711066337888	1.3163217937846165E-4	1.3163217937846165E-4	2.180157970955771E-4	4.5248561661346187E-4	8.227011211153853E-5
55-59	0.0047634394912580815	1.6454022422307706E-4	1.4808620180076936E-4	2.4269683072903865E-4	5.265287175138466E-4	8.227011211153853E-5
60-64	0.00523237913029385	1.9333476346211555E-4	1.7276723543423092E-4	3.126264260238464E-4	5.800042903863465E-4	1.0283764013942315E-4
65-69	0.005520324522684235	2.0978878588442324E-4	2.0978878588442324E-4	3.4964797647403875E-4	6.581608968923082E-4	1.2340516816730779E-4
70-74	0.008687723838978468	2.756048755736541E-4	2.5503734754576946E-4	3.4964797647403875E-4	7.075229641592313E-4	1.4397269619519242E-4
75-79	0.06124187145582928	3.085129204182695E-4	2.9205889799596176E-4	3.743290101075003E-4	7.692255482428852E-4	1.5219970740634628E-4
80-84	0.08375097412954621	3.249669428405772E-4	3.4553447086846183E-4	4.0723705495211574E-4	9.173117500436547E-4	1.6454022422307706E-4
85-89	0.08401423848830315	3.6198849329076955E-4	3.702155045019234E-4	4.5248561661346187E-4	0.001225824670461924	1.6454022422307706E-4
90-94	0.08443792956567756	4.1546406616326957E-4	3.90783032529808E-4	4.730531446413465E-4	0.0012546192097009625	1.6454022422307706E-4
95-99	0.08482048558699622	4.5248561661346187E-4	4.113505605576926E-4	5.01847683880385E-4	0.00127107323212327	1.6454022422307706E-4
100-104	0.08514133902423122	4.730531446413465E-4	4.113505605576926E-4	5.676637735696157E-4	0.0012957542657567318	1.6454022422307706E-4
105-109	0.0855732571128168	4.730531446413465E-4	4.319180885855773E-4	6.17025840836539E-4	0.0012957542657567318	1.6454022422307706E-4
110-114	0.08593935911171315	5.265287175138466E-4	4.565991222190388E-4	6.540473912867314E-4	0.0012957542657567318	1.6454022422307706E-4
115-119	0.08627255306576487	5.429827399361542E-4	4.895071670636543E-4	7.280904921871161E-4	0.0013080947825734627	1.6454022422307706E-4
120-124	0.0869841895355297	5.55323256752885E-4	5.01847683880385E-4	7.527715258205776E-4	0.001336889321812501	1.6454022422307706E-4
125-129	0.08825526276765296	5.923448072030775E-4	5.141882006971158E-4	7.733390538484622E-4	0.0013615703554459625	1.8099424664538478E-4
130-134	0.08943995238205911	6.334798632588466E-4	5.388692343305773E-4	8.021335930875007E-4	0.001435613456346347	1.851077522509617E-4
135-139	0.09094549543370026	6.375933688644236E-4	5.63550267964039E-4	8.021335930875007E-4	0.0014890890292188473	1.851077522509617E-4
140-144	0.09218366062097892	6.375933688644236E-4	6.087988296253851E-4	8.227011211153852E-4	0.0015590186245136553	1.851077522509617E-4
145-149	0.09331076115690701	6.375933688644236E-4	6.663879081034621E-4	8.268146267209623E-4	0.0015754726469359628	1.9333476346211555E-4
150-154	0.0940964407275722	6.417068744700006E-4	6.787284249201929E-4	8.638361771711546E-4	0.0016042671861750013	2.056752802788463E-4
155-159	0.09512070362336085	6.746149193146159E-4	6.787284249201929E-4	8.761766939878854E-4	0.0016207212085973092	2.056752802788463E-4
160-164	0.09631362024897816	6.951824473425006E-4	6.910689417369237E-4	8.885172108046161E-4	0.001674196781469809	2.056752802788463E-4
165-167	0.09707873229161547	6.992959529480775E-4	6.992959529480775E-4	9.186829185788469E-4	0.0017002489836384631	2.0567528027884633E-4
>>END_MODULE
