##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293726
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9987060
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.36845357893114	39.0	39.0	39.0	37.0	39.0
2	40.08944323955198	41.0	40.0	41.0	38.0	41.0
3	40.157189002569325	41.0	40.0	41.0	38.0	41.0
4	40.187501727235045	41.0	40.0	41.0	38.0	41.0
5	40.20056883607388	41.0	40.0	41.0	38.0	41.0
6	40.195620132451396	41.0	40.0	41.0	38.0	41.0
7	40.22173883004608	41.0	40.0	41.0	39.0	41.0
8	40.20419542888498	41.0	40.0	41.0	38.0	41.0
9	40.182926306640795	41.0	40.0	41.0	38.0	41.0
10-14	40.12895262469635	41.0	40.0	41.0	38.0	41.0
15-19	40.005826579594	41.0	40.0	41.0	38.0	41.0
20-24	39.834532645242945	41.0	40.0	41.0	37.8	41.0
25-29	39.610547368294576	40.4	39.4	41.0	36.8	41.0
30-34	39.44950866421149	40.0	39.0	41.0	35.8	41.0
35-39	39.14964151612186	40.0	38.2	41.0	35.0	41.0
40-44	38.676288317082296	40.0	37.0	41.0	35.0	41.0
45-49	38.208568327415676	39.2	35.6	41.0	35.0	41.0
50-54	37.52995061609723	37.6	35.0	40.4	34.4	41.0
55-59	36.79862818487122	36.4	35.0	39.2	34.0	41.0
60-64	35.835004355636194	35.2	34.6	37.4	33.4	40.0
65-69	35.50919411718765	35.0	34.8	36.4	33.6	39.0
70-74	35.329963953355644	35.0	35.0	36.0	34.0	37.2
75-79	35.127054949104135	35.0	35.0	35.2	34.0	36.8
80-84	35.03332045667093	35.0	35.0	35.0	33.2	36.0
85-89	34.897764166831884	35.0	34.8	35.0	32.8	36.0
90-94	39.70931121935404	40.6	39.8	40.6	37.8	40.6
95-99	40.03314346211994	41.0	40.0	41.0	38.0	41.0
100-104	39.927257763390045	41.0	40.0	41.0	38.0	41.0
105-109	39.78351945467283	41.0	40.0	41.0	37.0	41.0
110-114	39.60457280464543	41.0	39.4	41.0	36.4	41.0
115-119	39.343856570164846	40.0	38.8	41.0	35.2	41.0
120-124	39.08694746485876	40.0	38.0	41.0	35.0	41.0
125-129	38.42470171536563	39.4	37.0	40.4	34.4	40.6
130-134	38.37813554987683	39.6	36.2	40.8	35.0	41.0
135-139	37.87605321212104	38.8	35.0	41.0	34.4	41.0
140-144	37.15745124455411	37.0	35.0	39.8	34.0	41.0
145-149	36.51030796123446	36.0	35.0	38.8	34.0	41.0
150-154	35.94796853465833	35.0	35.0	37.0	34.0	39.2
155-159	35.38823791476521	35.0	35.0	36.0	34.0	37.6
160-164	35.00880751525463	35.0	35.0	35.4	34.0	36.4
165-169	34.788908631371676	35.0	35.0	35.0	34.0	36.0
170-174	34.67203934131989	35.0	35.0	35.0	33.4	36.0
175-178	34.43507957844535	35.0	34.75	35.0	32.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	10.0
30	186.0
31	2177.0
32	14315.0
33	60537.0
34	186503.0
35	498812.0
36	1393748.0
37	3089873.0
38	3804444.0
39	930713.0
40	5742.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.754420219764377	27.84001497938332	28.289716893660398	22.115847907191906
2	22.25275506505418	28.55689261904905	28.482185948617513	20.70816636727926
3	20.083848499958947	27.08066237711599	30.40602539686354	22.429463726061524
4	21.022873598436377	26.163525602129155	30.696311026468248	22.117289772966217
5	22.00090917647436	27.051584750667363	28.57839043722577	22.369115635632507
6	22.30154820337517	27.186008695251658	28.101733643334477	22.410709458038703
7	22.43296826092964	27.524126219327812	27.545764218899254	22.49714130084329
8	22.414664575961293	27.730963867244213	27.463177351492828	22.39119420530166
9	22.690932066093524	27.652902856296045	27.275124010469547	22.38104106714088
10-14	22.530450402821252	27.771352129655774	27.268439360532533	22.429758106990448
15-19	22.528133404625585	27.704235280452906	27.405072163379412	22.362559151542097
20-24	22.394844929338564	27.554805918859003	27.600126563773525	22.450222588028907
25-29	22.445961073629274	27.585918178122494	27.385048252438654	22.583072495809578
30-34	22.44477153436547	27.652298073707378	27.4353453368659	22.46758505506125
35-39	22.340021988453056	27.665206777570177	27.51009406171586	22.484677172260906
40-44	22.3864460612032	27.687239287638203	27.446565856217948	22.479748794940654
45-49	22.569432846102856	27.528333663760908	27.380988999765698	22.52124449037054
50-54	22.50515967662155	27.620689171788293	27.422508726291824	22.451642425298335
55-59	22.47061497577866	27.578438499418244	27.408869076585102	22.542077448217995
60-64	22.48346960967492	27.61658385951421	27.406876498188655	22.493070032622214
65-69	22.434233898664875	27.572761152931896	27.485087703488315	22.50791724491492
70-74	22.393220827751108	27.647579968479214	27.446317534890145	22.51288166887953
75-79	22.427959779955263	27.570594349087717	27.470665040562487	22.53078083039453
80-84	22.39412399645141	27.61971791498199	27.447861532823474	22.53829655574313
85-89	22.377925034995283	27.597687407505312	27.439486695784343	22.58490086171506
90-94	21.506565595985023	27.324049496164406	29.13799903755878	22.03138587029179
95-99	22.437447776648398	27.587466704278068	27.540101030069124	22.434984489004407
100-104	22.520480621280054	27.690932081668162	27.363316410882838	22.425270886168946
105-109	22.394817597956056	27.69279207707447	27.534401476842486	22.37798884812699
110-114	22.428205044847925	27.469553982757482	27.605508238689968	22.496732733704626
115-119	22.439580922094287	27.57882376389107	27.44405158506123	22.537543728953413
120-124	22.34543506426864	27.626125636044247	27.52025945017273	22.508179849514388
125-129	22.36111788414478	27.604918081020323	27.55012726459562	22.483836770239275
130-134	22.393088137558276	27.636425844416006	27.5193633573361	22.451122660689617
135-139	22.436488460240124	27.628138614006133	27.44596302771509	22.489409898038655
140-144	22.435770651175684	27.69876026091842	27.47886241598244	22.386606671923456
145-149	22.342442376657207	27.71719614713818	27.54802884456607	22.392332631638542
150-154	22.265164606278777	27.82172866549748	27.618416562606974	22.29469016561677
155-159	22.262145315764247	27.787154105929062	27.671969011086922	22.278731567219772
160-164	22.17501693217976	27.92211812452752	27.721914117402076	22.180950825890648
165-169	22.080609624034313	27.91857403336705	27.777853831232473	22.222962511366163
170-174	22.107793516592274	28.017379361818072	27.773498099825456	22.1013290217642
175-178	21.98655293882706	28.085271868575173	27.83218690799148	22.09598828460629
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	3.0
12	5.5
13	8.5
14	18.5
15	47.5
16	111.0
17	250.0
18	524.5
19	1057.5
20	2051.0
21	3902.0
22	7130.5
23	12159.5
24	19471.0
25	29316.5
26	42209.5
27	58274.0
28	76947.0
29	98457.0
30	122633.0
31	148948.5
32	176960.0
33	207146.5
34	239230.0
35	271488.5
36	303092.0
37	334554.5
38	364083.0
39	389138.0
40	410279.0
41	426473.5
42	436888.5
43	440425.5
44	436467.5
45	426116.0
46	410097.5
47	388379.5
48	362455.0
49	334783.0
50	305954.5
51	278029.5
52	251843.0
53	227182.5
54	205460.0
55	187181.5
56	172059.0
57	158839.0
58	147241.0
59	137479.0
60	128271.5
61	118905.5
62	109166.5
63	99297.5
64	89230.5
65	78237.5
66	67171.5
67	56650.5
68	46630.5
69	37447.0
70	29320.5
71	22382.0
72	16620.0
73	11948.0
74	8290.5
75	5685.0
76	3746.0
77	2407.0
78	1490.5
79	874.5
80	522.5
81	288.5
82	139.5
83	76.5
84	42.5
85	20.0
86	8.5
87	3.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	907.0
92-93	2074.0
94-95	3258.0
96-97	4279.0
98-99	5345.0
100-101	7821.0
102-103	7317.0
104-105	9159.0
106-107	8849.0
108-109	9870.0
110-111	10459.0
112-113	12118.0
114-115	11336.0
116-117	11875.0
118-119	12370.0
120-121	13250.0
122-123	13065.0
124-125	14021.0
126-127	14331.0
128-129	15177.0
130-131	15639.0
132-133	17284.0
134-135	88625.0
136-137	94204.0
138-139	90160.0
140-141	102474.0
142-143	95907.0
144-145	97439.0
146-147	87115.0
148-149	82184.0
150-151	82115.0
152-153	83025.0
154-155	74615.0
156-157	73984.0
158-159	84042.0
160-161	80209.0
162-163	81070.0
164-165	83313.0
166-167	88706.0
168-169	89562.0
170-171	92353.0
172-173	96663.0
174-175	103777.0
176-177	246993.0
178-179	7668721.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.22467438647264
#Duplication Level	Percentage of total
1	89.0986751917387
2	9.26521639136169
3	1.2072572704562772
4	0.2515915856692285
5	0.07340895357035723
6	0.030740166896483564
7	0.024715019742835277
8	0.016558873209178412
9	0.007280584403325335
>10	0.02455596295191336
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0025913532110553E-5	0.0	0.0	1.0012956766055276E-5	1.0012956766055276E-5	0.0
2	5.0064783830276375E-5	0.0	0.0	3.0038870298165825E-5	2.0025913532110553E-5	0.0
3	5.0064783830276375E-5	1.0012956766055276E-5	1.0012956766055276E-5	4.0051827064221105E-5	2.0025913532110553E-5	0.0
4	8.010365412844221E-5	2.0025913532110553E-5	2.0025913532110553E-5	4.0051827064221105E-5	2.0025913532110553E-5	0.0
5	8.010365412844221E-5	2.0025913532110553E-5	2.0025913532110553E-5	7.009069736238693E-5	3.0038870298165825E-5	0.0
6	8.010365412844221E-5	2.0025913532110553E-5	2.0025913532110553E-5	7.009069736238693E-5	3.0038870298165825E-5	0.0
7	8.010365412844221E-5	2.0025913532110553E-5	3.0038870298165825E-5	7.009069736238693E-5	3.0038870298165825E-5	0.0
8	8.010365412844221E-5	2.0025913532110553E-5	3.0038870298165825E-5	8.010365412844221E-5	3.0038870298165825E-5	0.0
9	8.010365412844221E-5	2.0025913532110553E-5	5.0064783830276375E-5	9.011661089449748E-5	4.0051827064221105E-5	0.0
10-14	1.2015548119266332E-4	3.6046644357799E-5	6.808810600917587E-5	1.2416066389908542E-4	4.0051827064221105E-5	0.0
15-19	1.3617621201835175E-4	4.205441841743216E-5	1.0813993307339698E-4	1.702202650229397E-4	4.0051827064221105E-5	0.0
20-24	1.6621508231651758E-4	5.406996653669849E-5	1.2616325525229648E-4	2.1627986614679396E-4	4.605960112385427E-5	0.0
25-29	1.8023322178899496E-4	8.611142818807537E-5	1.4819176013761807E-4	2.763576067431256E-4	5.006478383027637E-5	0.0
30-34	4.0252086199542205E-4	1.4618916878440702E-4	1.7622803908257285E-4	3.3643534733945727E-4	6.608551465596482E-5	8.01036541284422E-6
35-39	8.070443153440552E-4	1.742254477293618E-4	2.102720920871608E-4	3.62469034931201E-4	9.612438495413064E-5	1.4018139472477387E-5
40-44	0.0011414770713303014	2.18282457500005E-4	2.18282457500005E-4	4.125338187614774E-4	1.3617621201835175E-4	2.403109623853266E-5
45-49	0.0014799150100229697	2.623394672706482E-4	2.3830837103211558E-4	4.70608968004598E-4	1.9224876990826128E-4	3.204146165137688E-5
50-54	0.0017542700254128845	2.7836019809633665E-4	2.843679721559698E-4	5.206737518348743E-4	2.2429023155963817E-4	4.0051827064221105E-5
55-59	0.001918482516376191	2.803627894495477E-4	2.983861116284472E-4	5.607255788990954E-4	2.7435501538991455E-4	5.006478383027637E-5
60-64	0.0020846975986927086	2.8236538080275876E-4	3.063964770412914E-4	6.007774059633165E-4	3.3643534733945727E-4	5.006478383027637E-5
65-69	0.0022288841761239045	2.90375746215603E-4	3.2041461651376884E-4	6.408292330275377E-4	3.9250790522936673E-4	5.006478383027637E-5
70-74	0.004487807222545974	2.9638352027523614E-4	3.344327559862462E-4	7.109199303899246E-4	4.3656491500001E-4	5.006478383027637E-5
75-79	0.04769171307672128	3.0038870298165825E-4	3.564612608715678E-4	7.60984714220201E-4	4.70608968004598E-4	5.80751492431206E-5
80-84	0.06636187226270793	3.0839906839450246E-4	3.604664435779899E-4	7.990339499312109E-4	5.887618578440502E-4	8.010365412844221E-5
85-89	0.06648803551796026	3.5045348681193465E-4	3.704794003440452E-4	8.470961424082762E-4	8.250676375229547E-4	8.010365412844221E-5
90-94	0.06656813917208868	3.7248199169725624E-4	4.0652604470184416E-4	9.051712916513969E-4	8.310754115825878E-4	8.010365412844221E-5
95-99	0.06663021950403823	3.9451049658257784E-4	4.225467755275327E-4	9.492283014220402E-4	8.310754115825878E-4	8.010365412844221E-5
100-104	0.06668629206192814	4.0051827064221094E-4	4.4457528041285424E-4	0.001013311224724794	8.310754115825878E-4	8.010365412844221E-5
105-109	0.0667864216295887	4.125338187614774E-4	4.646011939449648E-4	0.0010854045134403919	8.310754115825878E-4	8.010365412844221E-5
110-114	0.06685250714424465	4.225467755275327E-4	4.806219247706532E-4	0.0011514900280963566	8.450935510550654E-4	8.010365412844221E-5
115-119	0.0669686574427309	4.4057009770643213E-4	5.026504296559749E-4	0.0011975496292202109	8.611142818807538E-4	8.010365412844221E-5
120-124	0.06725302541488687	4.4257268905964324E-4	5.146659777752413E-4	0.0012476144130504874	8.751324213532311E-4	8.010365412844221E-5
125-129	0.06783577949867128	4.5058305447248745E-4	5.226763431880854E-4	0.0012876662401147085	8.831427867660753E-4	8.010365412844221E-5
130-134	0.06833842992832725	4.5859341988533167E-4	5.427022567201959E-4	0.001341736206651407	8.971609262385527E-4	8.010365412844221E-5
135-139	0.0688550984974557	4.70608968004598E-4	5.507126221330401E-4	0.001375780259655995	9.191894311238742E-4	8.811401954128643E-5
140-144	0.06943985517259334	4.906348815367085E-4	5.667333529587286E-4	0.001415832086720216	9.51230892775251E-4	9.011661089449748E-5
145-149	0.06984037344323554	5.08658203715608E-4	5.80751492431206E-4	0.0014318528175459044	9.79267171720206E-4	9.011661089449748E-5
150-154	0.07007667922291445	5.206737518348743E-4	5.84756675137628E-4	0.001481917601376181	0.0010053008593119497	9.011661089449748E-5
155-159	0.07035303682965759	5.406996653669849E-4	5.907644491972612E-4	0.001519966837087191	0.0010333371382569042	9.011661089449748E-5
160-164	0.0707215136386484	5.487100307798291E-4	5.947696319036833E-4	0.001539992750619301	0.0010633760085550703	9.612438495413064E-5
165-167	0.07116875904086555	5.607255788990954E-4	6.107903627293718E-4	0.0015520082987385676	0.0010713863739679145	1.1681782893731155E-4
>>END_MODULE
