##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293727
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3423277
Total Bases	590.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.378577602688885	39.0	39.0	39.0	37.0	39.0
2	40.1002399163141	41.0	40.0	41.0	38.0	41.0
3	40.16981360258022	41.0	40.0	41.0	38.0	41.0
4	40.199514383440196	41.0	40.0	41.0	38.0	41.0
5	40.20741587665854	41.0	40.0	41.0	38.0	41.0
6	40.20208531182256	41.0	40.0	41.0	38.0	41.0
7	40.22517283877408	41.0	40.0	41.0	39.0	41.0
8	40.20409887952392	41.0	40.0	41.0	38.0	41.0
9	40.18946728529418	41.0	40.0	41.0	38.0	41.0
10-14	40.131457606264405	41.0	40.0	41.0	38.0	41.0
15-19	40.00739811589889	41.0	40.0	41.0	38.0	41.0
20-24	39.8286035865634	41.0	40.0	41.0	37.8	41.0
25-29	39.591352730147165	40.2	39.2	41.0	36.8	41.0
30-34	39.41273574998459	40.0	39.0	41.0	35.8	41.0
35-39	39.10311090805682	40.0	38.2	41.0	35.0	41.0
40-44	38.6199717989517	40.0	36.8	41.0	35.0	41.0
45-49	38.141295314401965	39.0	35.6	41.0	35.0	41.0
50-54	37.43748519328118	37.4	35.0	40.2	34.2	41.0
55-59	36.69335978362253	36.2	35.0	39.0	34.0	41.0
60-64	35.73826996763627	35.0	34.6	37.0	33.4	39.6
65-69	35.444903056340465	35.0	34.8	36.0	33.6	38.8
70-74	35.28986862588099	35.0	35.0	36.0	34.0	37.2
75-79	35.09865336635043	35.0	35.0	35.0	34.0	36.6
80-84	35.00288051478159	35.0	35.0	35.0	33.0	36.0
85-89	34.87242160070599	35.0	34.8	35.0	32.8	36.0
90-94	39.746197916548304	40.6	39.8	40.6	37.8	40.6
95-99	40.058081559203494	41.0	40.0	41.0	38.0	41.0
100-104	39.95249681473791	41.0	40.0	41.0	38.0	41.0
105-109	39.80194459249046	41.0	40.0	41.0	37.2	41.0
110-114	39.61884320001837	41.0	39.4	41.0	36.4	41.0
115-119	39.34565732639125	40.0	38.6	41.0	35.4	41.0
120-124	39.08029954190524	40.0	38.0	41.0	35.0	41.0
125-129	38.42148649674492	39.4	37.0	40.4	34.4	40.6
130-134	38.39285440778499	39.6	36.4	40.8	35.0	41.0
135-139	37.88699424433328	38.8	35.0	41.0	34.6	41.0
140-144	37.17060890100889	37.0	35.0	39.8	34.0	41.0
145-149	36.52723051173139	36.0	35.0	39.0	34.0	41.0
150-154	35.97095538676397	35.0	35.0	37.0	34.0	39.2
155-159	35.41069418298264	35.0	35.0	36.0	34.0	37.8
160-164	35.01700474726927	35.0	35.0	35.4	34.0	36.6
165-169	34.786081613232355	35.0	35.0	35.0	34.0	36.0
170-174	34.666076033448746	35.0	35.0	35.0	33.0	36.0
175-178	34.423957719171085	35.0	34.75	35.0	32.5	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	55.0
31	664.0
32	4431.0
33	18900.0
34	60470.0
35	169042.0
36	491888.0
37	1090291.0
38	1294803.0
39	291523.0
40	1208.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.813718258849633	27.790038609203986	28.27489566284002	22.121347469106357
2	22.28741641415521	28.50581475001877	28.455628919307436	20.75113991651859
3	20.144849511155538	27.03213908778051	30.313994456189203	22.509016944874748
4	21.143570911731654	26.088335825584668	30.589695195568456	22.178398067115225
5	22.07022686157153	26.947483361702833	28.555679251197024	22.42661052552861
6	22.336842738697452	27.135986950515544	28.036293878643182	22.490876432143818
7	22.515940135723753	27.481445410348037	27.446478914794213	22.556135539133994
8	22.503408283933787	27.683123510016866	27.38525103285536	22.42821717319399
9	22.746041293181943	27.619412627140605	27.208373730784857	22.426172348892596
10-14	22.601261890288164	27.684362089307996	27.2093143499635	22.50506167044034
15-19	22.585873126831395	27.62795415036528	27.359305133648252	22.42686758915507
20-24	22.449652774227737	27.502542154783267	27.527687651335253	22.52011741965374
25-29	22.476013480650266	27.538145467048093	27.325571375030417	22.660269677271224
30-34	22.499260211779532	27.592035350922522	27.391332924563216	22.517371512734726
35-39	22.368064284602152	27.601686921625095	27.462305854887	22.56794293888575
40-44	22.458761005901657	27.622030002246383	27.379169141147504	22.540039850704456
45-49	22.63442894045676	27.45202915218371	27.315732848963144	22.597809058396383
50-54	22.576957692877322	27.57707307939147	27.3445415021922	22.50142772553901
55-59	22.530312329384973	27.50926086320213	27.342742056806973	22.617684750605925
60-64	22.568235056643093	27.528277729205087	27.359550512564425	22.5439367015874
65-69	22.487043847167495	27.522820969497943	27.416414155208592	22.573721028125973
70-74	22.45429744656947	27.57811301860761	27.368501000649374	22.599088534173543
75-79	22.505920496646926	27.498201284909168	27.392518922658027	22.60335929578588
80-84	22.473939444573137	27.56139804053251	27.359106493573265	22.605556021321092
85-89	22.4467666507852	27.53599548035406	27.376277175349816	22.640960693510927
90-94	21.56180535985599	27.253011432918946	29.079103383926412	22.106079823298654
95-99	22.49811409097665	27.523829224988745	27.471692654654774	22.50636402937984
100-104	22.586993107998886	27.63969673204451	27.271229503373696	22.50208065658291
105-109	22.461536584287007	27.62413061258587	27.465174345622405	22.449158457504716
110-114	22.486281860335634	27.408493447148224	27.54097182605697	22.56425286645917
115-119	22.49033712025841	27.488193744645244	27.39241109413576	22.629058040960587
120-124	22.399869127597754	27.56594601679009	27.431684700656582	22.602500154955575
125-129	22.42732370455892	27.539700417381923	27.473434153159037	22.559541724900118
130-134	22.456320412876416	27.55286494368757	27.460296564307324	22.53051807912869
135-139	22.516473645039937	27.5450022864149	27.38357166001582	22.554952408529346
140-144	22.515253125932833	27.612673921045396	27.43319843369999	22.438874519321782
145-149	22.42329219421145	27.64607121343698	27.472605578975667	22.458031013375905
150-154	22.320557891240504	27.75117038894543	27.570349938958245	22.357921780855822
155-159	22.325139184171903	27.734307802076373	27.625543185028654	22.315009828723074
160-164	22.251585473338	27.861068094523887	27.63855799640543	22.248788435732678
165-169	22.151641260572923	27.85334671971269	27.718192572390926	22.276819447323458
170-174	22.173142313908897	27.950862474523948	27.70219722131493	22.173797990252233
175-178	22.090105192774256	28.042247584608987	27.7463005702717	22.121346652345057
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	1.5
13	3.5
14	10.0
15	15.5
16	31.5
17	70.5
18	147.0
19	343.0
20	683.5
21	1300.0
22	2331.5
23	3858.5
24	6333.5
25	9712.5
26	13974.5
27	19265.5
28	25700.0
29	32930.0
30	40890.0
31	49868.5
32	59601.0
33	69780.0
34	80642.0
35	91666.0
36	102500.0
37	113482.5
38	123718.5
39	132785.0
40	139883.0
41	145547.5
42	149492.5
43	150409.5
44	148899.0
45	145586.0
46	140812.5
47	133520.0
48	124802.5
49	115586.0
50	105762.0
51	96074.5
52	87064.5
53	79111.5
54	71759.0
55	65415.5
56	60088.5
57	55427.5
58	51442.5
59	47888.0
60	44639.0
61	41593.5
62	38316.5
63	34918.5
64	31224.0
65	27218.5
66	23444.0
67	19879.5
68	16379.0
69	13244.5
70	10476.0
71	7866.0
72	5739.0
73	4151.5
74	2922.0
75	1954.0
76	1270.5
77	849.5
78	526.5
79	289.0
80	174.0
81	98.5
82	48.5
83	25.0
84	10.0
85	5.5
86	2.5
87	2.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	283.0
92-93	637.0
94-95	981.0
96-97	1395.0
98-99	1739.0
100-101	2143.0
102-103	2568.0
104-105	2727.0
106-107	3080.0
108-109	3251.0
110-111	3483.0
112-113	3488.0
114-115	3739.0
116-117	3806.0
118-119	4078.0
120-121	4274.0
122-123	4286.0
124-125	4547.0
126-127	4840.0
128-129	4974.0
130-131	5319.0
132-133	5912.0
134-135	28199.0
136-137	30577.0
138-139	30728.0
140-141	31338.0
142-143	31788.0
144-145	33282.0
146-147	29432.0
148-149	28370.0
150-151	27351.0
152-153	27089.0
154-155	24224.0
156-157	24599.0
158-159	25156.0
160-161	25912.0
162-163	28030.0
164-165	28315.0
166-167	28522.0
168-169	30798.0
170-171	31462.0
172-173	33031.0
174-175	35660.0
176-177	80675.0
178-179	2657189.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.7980411462759
#Duplication Level	Percentage of total
1	95.71550714722457
2	3.9784900248916477
3	0.2346412997310683
4	0.04072601786069915
5	0.011654827648013845
6	0.008599577174846286
7	0.004362035241149433
8	0.0011062146112736902
9	0.0
>10	0.004912855616735328
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	2.9211775734186863E-5	0.0	0.0	0.0
2	0.0	2.9211775734186863E-5	2.9211775734186863E-5	0.0	0.0	0.0
3	0.0	2.9211775734186863E-5	2.9211775734186863E-5	0.0	0.0	0.0
4	0.0	2.9211775734186863E-5	2.9211775734186863E-5	2.9211775734186863E-5	0.0	0.0
5	0.0	2.9211775734186863E-5	2.9211775734186863E-5	2.9211775734186863E-5	0.0	0.0
6	0.0	2.9211775734186863E-5	2.9211775734186863E-5	2.9211775734186863E-5	0.0	0.0
7	0.0	5.8423551468373726E-5	2.9211775734186863E-5	2.9211775734186863E-5	2.9211775734186863E-5	0.0
8	0.0	5.8423551468373726E-5	2.9211775734186863E-5	5.8423551468373726E-5	2.9211775734186863E-5	0.0
9	0.0	8.763532720256059E-5	5.8423551468373726E-5	5.8423551468373726E-5	2.9211775734186863E-5	0.0
10-14	4.673884117469898E-5	9.347768234939796E-5	5.8423551468373726E-5	8.763532720256059E-5	2.9211775734186863E-5	0.0
15-19	9.347768234939796E-5	1.1684710293674745E-4	5.8423551468373726E-5	1.519012338177717E-4	2.9211775734186863E-5	0.0
20-24	1.1684710293674745E-4	1.1684710293674745E-4	8.179297205572322E-5	2.0448243013930804E-4	2.9211775734186863E-5	0.0
25-29	1.1684710293674745E-4	1.1684710293674745E-4	1.1100474778991008E-4	2.0448243013930804E-4	5.8423551468373726E-5	0.0
30-34	2.8627540219503125E-4	1.4021652352409693E-4	1.4605887867093431E-4	2.0448243013930804E-4	5.8423551468373726E-5	0.0
35-39	7.06924972767322E-4	1.9864007499247066E-4	1.7527065440512118E-4	2.1032478528614542E-4	5.8423551468373726E-5	0.0
40-44	0.0010107274404028653	2.336942058734949E-4	1.8111300955195856E-4	2.9211775734186863E-4	7.595061690888585E-5	0.0
45-49	0.0013086875528915715	2.336942058734949E-4	2.0448243013930804E-4	3.5054130881024236E-4	2.2200949557982016E-4	2.336942058734949E-5
50-54	0.0015540664690587408	2.9211775734186863E-4	2.0448243013930804E-4	4.089648602786161E-4	3.271718882228929E-4	2.9211775734186863E-5
55-59	0.001653386506554976	3.096448227823807E-4	2.2200949557982016E-4	4.849154771875019E-4	3.5054130881024236E-4	2.9211775734186863E-5
60-64	0.001770233609491724	3.5054130881024236E-4	2.336942058734949E-4	5.433390286558756E-4	3.7391072939759183E-4	2.9211775734186863E-5
65-69	0.001910450133015821	4.089648602786161E-4	2.336942058734949E-4	5.550237389495504E-4	3.91437794838104E-4	2.9211775734186863E-5
70-74	0.004042909761611461	4.673884117469898E-4	2.4537891616716967E-4	5.783931595368999E-4	4.615460566001524E-4	2.9211775734186863E-5
75-79	0.04848570536360335	4.673884117469898E-4	2.9211775734186863E-4	6.134472904179241E-4	5.199696080685262E-4	2.9211775734186863E-5
80-84	0.0680342256849212	4.966001874811767E-4	2.9211775734186863E-4	6.543437764457858E-4	6.309743558584362E-4	2.9211775734186863E-5
85-89	0.06811017630183008	4.966001874811767E-4	2.9211775734186863E-4	7.536638139420211E-4	9.172497580534674E-4	2.9211775734186863E-5
90-94	0.06812186101212377	5.02442542628014E-4	3.096448227823807E-4	7.887179448230454E-4	9.347768234939796E-4	2.9211775734186863E-5
95-99	0.06814523043271112	5.258119632153635E-4	3.213295330760555E-4	7.945602999698826E-4	9.347768234939796E-4	2.9211775734186863E-5
100-104	0.06820365398417949	5.258119632153635E-4	3.5054130881024236E-4	8.296144308509069E-4	9.347768234939796E-4	2.9211775734186863E-5
105-109	0.06827960460108838	5.783931595368999E-4	3.5054130881024236E-4	8.471414962914189E-4	9.464615337876544E-4	2.9211775734186863E-5
110-114	0.06830881637682255	6.42659066152111E-4	3.5054130881024236E-4	8.705109168787685E-4	9.639885992281664E-4	2.9211775734186863E-5
115-119	0.06837308228343777	6.42659066152111E-4	3.6806837425075445E-4	9.581462440813291E-4	9.639885992281664E-4	2.9211775734186863E-5
120-124	0.06865351533048596	6.42659066152111E-4	3.797530845444292E-4	0.001051623926430727	9.639885992281664E-4	2.9211775734186863E-5
125-129	0.06915595787311399	6.42659066152111E-4	3.972801499849413E-4	0.0011217321881927754	9.639885992281664E-4	2.9211775734186863E-5
130-134	0.06982782871500029	6.543437764457858E-4	4.089648602786161E-4	0.0012444216462763602	9.639885992281664E-4	2.9211775734186863E-5
135-139	0.0705698078186486	6.718708418862978E-4	4.3233428086596556E-4	0.0012677910668637098	0.0010107274404028653	2.9211775734186863E-5
140-144	0.07142863402523372	6.718708418862978E-4	4.498613463064777E-4	0.0012911604874510593	0.0010224121506965402	5.8423551468373726E-5
145-149	0.07166817058625405	6.718708418862978E-4	4.673884117469898E-4	0.0013787958146536199	0.0010633086367244017	5.8423551468373726E-5
150-154	0.07193691892300856	6.718708418862978E-4	5.491813838027131E-4	0.0014722734970030178	0.0011217321881927754	8.763532720256059E-5
155-159	0.07241014968990239	6.718708418862978E-4	5.725508043900625E-4	0.0015423817587650662	0.0011392592536332877	8.763532720256059E-5
160-164	0.0729418040082646	7.06924972767322E-4	6.076049352710868E-4	0.0016124900205271148	0.001145101608780125	8.763532720256059E-5
165-167	0.07346761597147995	7.78980686244983E-4	6.329218075740487E-4	0.0016358594411144643	0.0011684710293674745	8.763532720256059E-5
>>END_MODULE
