##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293728
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10004012
Total Bases	1.7 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.37307662165939	39.0	39.0	39.0	37.0	39.0
2	40.08911234812593	41.0	40.0	41.0	38.0	41.0
3	40.147575992511804	41.0	40.0	41.0	38.0	41.0
4	40.17950308336295	41.0	40.0	41.0	38.0	41.0
5	40.185289361908005	41.0	40.0	41.0	38.0	41.0
6	40.186545757841955	41.0	40.0	41.0	38.0	41.0
7	40.21353113131012	41.0	40.0	41.0	39.0	41.0
8	40.1955990256709	41.0	40.0	41.0	38.0	41.0
9	40.17605946494267	41.0	40.0	41.0	38.0	41.0
10-14	40.12223375981556	41.0	40.0	41.0	38.0	41.0
15-19	40.000830686728484	41.0	40.0	41.0	38.0	41.0
20-24	39.82594750985904	41.0	40.0	41.0	37.8	41.0
25-29	39.59844438411309	40.2	39.2	41.0	36.8	41.0
30-34	39.43073320983621	40.0	39.0	41.0	35.8	41.0
35-39	39.12923103250976	40.0	38.2	41.0	35.0	41.0
40-44	38.647810958243554	40.0	37.0	41.0	35.0	41.0
45-49	38.18161229714639	39.2	35.6	41.0	35.0	41.0
50-54	37.49817663153543	37.6	35.0	40.4	34.4	41.0
55-59	36.76270946096427	36.4	35.0	39.2	34.0	41.0
60-64	35.817124149791105	35.2	34.6	37.4	33.4	40.0
65-69	35.485663791686775	35.0	34.8	36.2	33.6	39.0
70-74	35.30880774633217	35.0	35.0	36.0	34.0	37.2
75-79	35.106304850493984	35.0	35.0	35.2	34.0	36.8
80-84	35.01321941637015	35.0	35.0	35.0	33.0	36.0
85-89	34.878372896793806	35.0	34.8	35.0	32.8	36.0
90-94	39.706780793159666	40.6	39.8	40.6	37.8	40.6
95-99	40.027310674861425	41.0	40.0	41.0	38.0	41.0
100-104	39.92120271729725	41.0	40.0	41.0	38.0	41.0
105-109	39.77250970973045	41.0	40.0	41.0	37.0	41.0
110-114	39.59448798549952	41.0	39.4	41.0	36.4	41.0
115-119	39.334171110762924	40.0	38.6	41.0	35.2	41.0
120-124	39.076974852975795	40.0	38.0	41.0	35.0	41.0
125-129	38.430194279730756	39.4	37.0	40.4	34.4	40.6
130-134	38.36461139495341	39.4	36.2	40.8	35.0	41.0
135-139	37.84752368527376	38.8	35.0	41.0	34.4	41.0
140-144	37.133601820413084	37.0	35.0	39.8	34.0	41.0
145-149	36.48873347981993	36.0	35.0	38.8	34.0	40.8
150-154	35.92875304923061	35.0	35.0	37.0	34.0	39.2
155-159	35.371257310282395	35.0	35.0	36.0	34.0	37.6
160-164	34.99142838369141	35.0	35.0	35.4	34.0	36.4
165-169	34.77198058308369	35.0	35.0	35.0	33.8	36.0
170-174	34.657373863036064	35.0	35.0	35.0	33.0	36.0
175-178	34.42286502956004	35.0	34.75	35.0	32.5	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	7.0
30	172.0
31	2240.0
32	14645.0
33	61784.0
34	190559.0
35	508501.0
36	1419685.0
37	3127182.0
38	3782379.0
39	892605.0
40	4253.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.74057768023469	27.81173193314842	28.309802107394518	22.137888279222377
2	22.24643473038617	28.53703094318559	28.48394224237236	20.732592084055877
3	20.088290577820178	27.077716420172226	30.387958351109535	22.44603465089806
4	21.06111028255464	26.171879841807467	30.65352180705101	22.113488068586882
5	22.019415810376877	27.043440171803073	28.57934396720036	22.35780005061969
6	22.31019914810178	27.205745055083902	28.058722840396435	22.425332956417883
7	22.454521246076073	27.503995397046705	27.550436764769977	22.491046592107246
8	22.409329377053925	27.71294156784298	27.478585591460707	22.39914346364239
9	22.717135885082904	27.635672568165653	27.26890971342297	22.37828183332847
10-14	22.55035279845726	27.758939113627612	27.261262781372114	22.429445306543013
15-19	22.551396379772434	27.69504874644293	27.38215427970298	22.371400594081656
20-24	22.408657646552204	27.546518336843256	27.589679020776863	22.455144995827673
25-29	22.442522060149468	27.574055289018045	27.377530134909872	22.605892515922612
30-34	22.455292936473885	27.63249384347	27.426222599493084	22.48599062056303
35-39	22.345590948911298	27.647857679498983	27.49977708943172	22.506774282158
40-44	22.395784811133773	27.6756905129662	27.41914144045409	22.50938323544594
45-49	22.588777382514134	27.52084263793366	27.36186841839054	22.52851156116166
50-54	22.51751197419595	27.625044832013394	27.40156449232568	22.455878701464975
55-59	22.493439632019633	27.554874984156356	27.40042694870818	22.55125843511583
60-64	22.50907335976806	27.600448699981566	27.395480933049658	22.494997007200713
65-69	22.436424506488	27.580544685472187	27.461094608842934	22.521936199196883
70-74	22.415370953173586	27.62874134897079	27.417334165532786	22.538553532322833
75-79	22.4325720520927	27.55976302307514	27.452542040133498	22.55512288469866
80-84	22.397813997024393	27.6203487160951	27.44378355403812	22.538053732842382
85-89	22.382546122495654	27.598579449924692	27.418791580817775	22.60008284676188
90-94	21.506138125043524	27.301917606611394	29.131729462510346	22.06021480583473
95-99	22.44388547427545	27.568788895855175	27.53136303851942	22.455962591349955
100-104	22.528639572470667	27.688308883211455	27.344706144712315	22.438345399605563
105-109	22.396607173007553	27.6831482325061	27.520037727523043	22.400206866963302
110-114	22.42094907463083	27.460694702291764	27.59787476667947	22.52048145639794
115-119	22.439003307414847	27.565783976783152	27.4397763584305	22.5554363573715
120-124	22.36414894504154	27.607796374291965	27.495547276267974	22.532507404398523
125-129	22.37650311393825	27.59712659086929	27.532831596657264	22.4935386985352
130-134	22.40592194902778	27.615812961122217	27.5172367589026	22.461028330947403
135-139	22.446497456939973	27.60488954673903	27.438267125158184	22.510345871162812
140-144	22.457633836416967	27.66355332983443	27.486460317928245	22.392352515820356
145-149	22.355671067517385	27.697824958008464	27.546554627989188	22.399949346484956
150-154	22.26949318578421	27.804649214028597	27.60607431634119	22.319783283846
155-159	22.27511859876258	27.769374947525822	27.662541223201714	22.29296523050988
160-164	22.18327037109184	27.924902181122995	27.696347916659775	22.195479531125393
165-169	22.08732393310132	27.920430832067556	27.751361429916134	22.24088380491499
170-174	22.109101824021923	28.008212704419044	27.757596032243132	22.125089439315904
175-178	21.992687563680878	28.06963278755905	27.83249745466711	22.10518219409296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	3.0
12	6.0
13	10.5
14	24.0
15	59.0
16	135.5
17	253.5
18	511.0
19	1056.5
20	2107.5
21	3949.0
22	7045.0
23	12058.5
24	19510.0
25	29226.5
26	41762.0
27	58060.5
28	77273.5
29	98450.0
30	122414.0
31	148699.5
32	176995.0
33	207324.0
34	238558.5
35	270563.5
36	303086.5
37	334779.0
38	363991.0
39	389804.0
40	411025.5
41	427644.0
42	437845.0
43	440115.0
44	436450.0
45	426389.5
46	409982.0
47	388661.5
48	363386.0
49	335181.0
50	306260.5
51	278558.5
52	251884.5
53	227447.0
54	206022.0
55	187609.5
56	172039.0
57	159349.0
58	148489.0
59	138519.5
60	129381.0
61	119984.0
62	110387.0
63	100306.0
64	89597.0
65	78679.0
66	67910.0
67	57515.0
68	47312.5
69	37909.0
70	29677.0
71	22583.0
72	16729.5
73	12093.5
74	8575.0
75	5856.0
76	3775.5
77	2376.5
78	1494.0
79	892.5
80	495.5
81	268.0
82	140.5
83	71.5
84	38.5
85	15.0
86	4.5
87	3.5
88	3.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	873.0
92-93	1952.0
94-95	3134.0
96-97	4310.0
98-99	5310.0
100-101	6371.0
102-103	7348.0
104-105	8085.0
106-107	9039.0
108-109	10013.0
110-111	10533.0
112-113	11328.0
114-115	12384.0
116-117	11873.0
118-119	12319.0
120-121	12531.0
122-123	13203.0
124-125	13952.0
126-127	14556.0
128-129	15129.0
130-131	15781.0
132-133	17567.0
134-135	86307.0
136-137	91885.0
138-139	92022.0
140-141	92170.0
142-143	94232.0
144-145	101542.0
146-147	88962.0
148-149	82336.0
150-151	83563.0
152-153	82643.0
154-155	75975.0
156-157	73748.0
158-159	76612.0
160-161	77808.0
162-163	82067.0
164-165	85383.0
166-167	86466.0
168-169	91663.0
170-171	93830.0
172-173	98202.0
174-175	107158.0
176-177	216478.0
178-179	7725369.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.28754860308017
#Duplication Level	Percentage of total
1	89.20129340371307
2	9.231068340602882
3	1.1416367390103952
4	0.2394141422566535
5	0.09277863773093119
6	0.029714589416557455
7	0.020595066739821942
8	0.007244177046719099
9	0.012480339678992087
>10	0.023774563803998932
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	9.995989608968881E-6	9.995989608968881E-6	0.0	9.995989608968881E-6	0.0	0.0
2	3.9983958435875525E-5	9.995989608968881E-6	0.0	1.9991979217937763E-5	0.0	0.0
3	3.9983958435875525E-5	1.9991979217937763E-5	0.0	3.9983958435875525E-5	0.0	0.0
4	3.9983958435875525E-5	1.9991979217937763E-5	0.0	5.997593765381329E-5	0.0	0.0
5	3.9983958435875525E-5	1.9991979217937763E-5	0.0	6.997192726278217E-5	0.0	0.0
6	3.9983958435875525E-5	1.9991979217937763E-5	0.0	6.997192726278217E-5	0.0	0.0
7	4.997994804484441E-5	1.9991979217937763E-5	0.0	7.996791687175105E-5	9.995989608968881E-6	0.0
8	4.997994804484441E-5	2.9987968826906645E-5	0.0	7.996791687175105E-5	9.995989608968881E-6	0.0
9	5.997593765381329E-5	3.9983958435875525E-5	0.0	7.996791687175105E-5	9.995989608968881E-6	0.0
10-14	6.997192726278217E-5	5.3978343888431956E-5	2.199117713973154E-5	1.0795668777686391E-4	1.3994385452556434E-5	0.0
15-19	9.596150024610127E-5	5.9975937653813284E-5	3.9983958435875525E-5	1.4993984413453324E-4	1.9991979217937763E-5	0.0
20-24	1.2395027115121412E-4	6.397433349740085E-5	3.9983958435875525E-5	1.9392219841399632E-4	1.9991979217937763E-5	0.0
25-29	1.4794064621273943E-4	8.396631271533861E-5	4.99799480448444E-5	1.999197921793776E-4	2.199117713973154E-5	0.0
30-34	2.4790054230242823E-4	1.1795267738583281E-4	7.996791687175105E-5	2.4190294853704692E-4	3.798476051408175E-5	3.998395843587553E-6
35-39	6.577361162701524E-4	1.1995187530762657E-4	9.196310440251371E-5	2.778885111293349E-4	6.397433349740085E-5	1.7992781296143985E-5
40-44	0.0010635732943942888	1.259494690730079E-4	1.4394225036915188E-4	3.3986364670494197E-4	1.2195107322942034E-4	3.598556259228797E-5
45-49	0.0013794465660377055	1.3794465660377055E-4	1.4993984413453322E-4	3.9184279267158017E-4	1.6793262543067722E-4	4.99799480448444E-5
50-54	0.0016113535249657838	1.4993984413453322E-4	1.6193503166529586E-4	4.398235427946308E-4	2.259093651626967E-4	8.396631271533861E-5
55-59	0.0018432604838938618	1.5593743789991455E-4	1.6993182335247098E-4	4.997994804484441E-4	2.638941256767785E-4	8.996390648071993E-5
60-64	0.0019991979217937766	1.8192701088323365E-4	1.7393021919605856E-4	5.757690014766075E-4	3.278684591741793E-4	9.99598960896888E-5
65-69	0.002115151401257815	2.0391818802296518E-4	1.8192701088323363E-4	6.237497515996582E-4	3.618548238446735E-4	9.99598960896888E-5
70-74	0.004208311625375899	2.0991578178834654E-4	1.8992380257040875E-4	6.717305017227089E-4	3.7784840721902366E-4	9.99598960896888E-5
75-79	0.04507191714684069	2.259093651626967E-4	2.119149797101403E-4	7.097152622367906E-4	4.0383798020234284E-4	1.0595748985507015E-4
80-84	0.0623809727537312	2.49899740224222E-4	2.3990375061525314E-4	7.377040331419036E-4	5.17792261744588E-4	1.099558856986577E-4
85-89	0.06248493104566448	3.0587728203444774E-4	2.459013443806345E-4	7.856847832649541E-4	6.757288975662964E-4	1.099558856986577E-4
90-94	0.06253890938955291	3.1787246956521047E-4	2.638941256767785E-4	8.096751583264795E-4	6.797272934098839E-4	1.099558856986577E-4
95-99	0.0626048829209721	3.238700633305917E-4	2.8788450073830376E-4	8.21670345857242E-4	6.797272934098839E-4	1.099558856986577E-4
100-104	0.0626748548482349	3.4786043839211706E-4	3.0187888619086017E-4	8.616543042931177E-4	6.877240850970591E-4	1.099558856986577E-4
105-109	0.06275282356718484	3.798476051408175E-4	3.198716674870042E-4	8.996390648071994E-4	6.997192726278217E-4	1.099558856986577E-4
110-114	0.06279080832769893	3.898435947497864E-4	3.618548238446735E-4	9.396230232430749E-4	7.137136580803782E-4	1.099558856986577E-4
115-119	0.06284278747366556	4.198315635766931E-4	3.858451989061988E-4	9.83605377522538E-4	7.277080435329345E-4	1.099558856986577E-4
120-124	0.06315066395362182	4.438219386382183E-4	4.258291573420744E-4	0.001035584523489176	7.536976165162536E-4	1.099558856986577E-4
125-129	0.0636684562153664	4.5581712616898104E-4	4.538179282471872E-4	0.0011035572528301646	7.816863874213666E-4	1.1995187530762657E-4
130-134	0.06421423724801609	4.5981552201256856E-4	4.5981552201256856E-4	0.0011915219613890906	8.036775645610981E-4	1.2994786491659545E-4
135-139	0.06476201747858759	4.7181070954333123E-4	4.5981552201256856E-4	0.0012295067219031727	8.256687417008297E-4	1.2994786491659545E-4
140-144	0.06540975760524878	4.8380589707409385E-4	4.6981151162153745E-4	0.0012974794512441608	8.296671375444172E-4	1.2994786491659545E-4
145-149	0.06588156831479211	5.037978762920316E-4	4.898034908394752E-4	0.0013214698263056862	8.49659116762355E-4	1.2994786491659545E-4
150-154	0.06609348329450224	5.317866471971445E-4	5.377842409625259E-4	0.0013594545868197679	8.596551063713238E-4	1.2994786491659545E-4
155-159	0.066493322878861	5.437818347279071E-4	5.717706056330201E-4	0.0013934409514902621	8.656527001367051E-4	1.2994786491659545E-4
160-164	0.06694714080710819	5.657730118676386E-4	5.797673973201951E-4	0.0014594144829094566	8.836454814328491E-4	1.359454586819768E-4
165-167	0.06748958984322156	5.89763386929164E-4	5.797673973201951E-4	0.0014694104725184256	8.929750717345534E-4	1.3994385452556433E-4
>>END_MODULE
