##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293729
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8353395
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.38706023119941	39.0	39.0	39.0	37.0	39.0
2	40.09835581820326	41.0	40.0	41.0	38.0	41.0
3	40.15968908449798	41.0	40.0	41.0	38.0	41.0
4	40.19357602507723	41.0	40.0	41.0	38.0	41.0
5	40.20619688162717	41.0	40.0	41.0	38.0	41.0
6	40.204791704450706	41.0	40.0	41.0	38.0	41.0
7	40.22942025368129	41.0	40.0	41.0	39.0	41.0
8	40.20353197711829	41.0	40.0	41.0	38.0	41.0
9	40.1892751390303	41.0	40.0	41.0	38.0	41.0
10-14	40.13676729042503	41.0	40.0	41.0	38.0	41.0
15-19	40.01289111792271	41.0	40.0	41.0	38.0	41.0
20-24	39.84260765832335	41.0	40.0	41.0	37.8	41.0
25-29	39.613220540869904	40.4	39.4	41.0	36.8	41.0
30-34	39.442593125310125	40.0	39.0	41.0	35.8	41.0
35-39	39.13037049008218	40.0	38.2	41.0	35.0	41.0
40-44	38.64519903584112	40.0	36.8	41.0	35.0	41.0
45-49	38.16447592864937	39.0	35.6	41.0	35.0	41.0
50-54	37.472403232458184	37.6	35.0	40.2	34.4	41.0
55-59	36.72923109705694	36.2	35.0	39.0	34.0	41.0
60-64	35.791068134572825	35.2	34.6	37.4	33.4	39.6
65-69	35.46405352554261	35.0	34.8	36.2	33.6	39.0
70-74	35.2912990706174	35.0	35.0	36.0	34.0	37.2
75-79	35.0983008704844	35.0	35.0	35.0	34.0	36.6
80-84	35.00618333025076	35.0	35.0	35.0	33.0	36.0
85-89	34.87456678392438	35.0	34.8	35.0	32.8	36.0
90-94	39.72544192557895	40.6	39.8	40.6	37.8	40.6
95-99	40.04077252400633	41.0	40.0	41.0	38.0	41.0
100-104	39.93772808317114	41.0	40.0	41.0	38.0	41.0
105-109	39.78383315468071	41.0	40.0	41.0	37.0	41.0
110-114	39.60724537575925	41.0	39.4	41.0	36.4	41.0
115-119	39.349973356207535	40.0	38.8	41.0	35.4	41.0
120-124	39.09243550813451	40.0	38.0	41.0	35.0	41.0
125-129	38.44456076058013	39.4	37.0	40.4	34.4	40.6
130-134	38.38406007112404	39.6	36.4	40.8	35.0	41.0
135-139	37.86983085991741	38.8	35.0	41.0	34.4	41.0
140-144	37.14797429817203	37.0	35.0	39.8	34.0	41.0
145-149	36.50005928921539	36.0	35.0	38.8	34.0	40.8
150-154	35.93665734439206	35.0	35.0	37.0	34.0	39.2
155-159	35.38086630464228	35.0	35.0	36.0	34.0	37.6
160-164	34.99631545429112	35.0	35.0	35.4	34.0	36.4
165-169	34.77665104477286	35.0	35.0	35.0	34.0	36.0
170-174	34.65492352782176	35.0	35.0	35.0	33.0	36.0
175-178	34.42294227442915	35.0	34.75	35.0	32.5	35.25
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	7.0
30	171.0
31	1801.0
32	11380.0
33	49417.0
34	153270.0
35	415219.0
36	1183480.0
37	2634805.0
38	3175152.0
39	725743.0
40	2950.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.756052479261427	27.793011105065663	28.286463168567987	22.16447324710492
2	22.297520948069618	28.51350857944584	28.471070744290195	20.717899728194343
3	20.113726215508784	27.02823223372054	30.37623624885451	22.481805301916165
4	21.07461696711337	26.09468365856038	30.658744139358905	22.17195523496734
5	22.032838145448647	27.022533951764522	28.56320095003289	22.38142695275394
6	22.346459134280135	27.15176284612424	28.06505618374326	22.436721835852367
7	22.47293465710648	27.51026379094967	27.498316552730955	22.518484999212895
8	22.474263458150848	27.703993406273735	27.394454590019983	22.42728854555543
9	22.732649419786803	27.65018294956721	27.230533214339797	22.386634416306183
10-14	22.5673465698677	27.732882259249088	27.23638472740724	22.463386443475976
15-19	22.54738821760494	27.670057503565914	27.386589524379012	22.395964754450137
20-24	22.427065881596643	27.52399473507478	27.568544286484713	22.480395096843857
25-29	22.451123166089957	27.571333571559826	27.359381425157075	22.618161837193142
30-34	22.47621715482148	27.61484881296766	27.406828002267343	22.502106029943512
35-39	22.358648190346557	27.641053727257002	27.486256785414792	22.514041296981645
40-44	22.411474616009418	27.671532353013355	27.402252617049715	22.514740413927512
45-49	22.607526640365982	27.50999324226856	27.33548934295577	22.546990774409686
50-54	22.53666203980537	27.599738788839744	27.383783479651086	22.47981569170379
55-59	22.494516301455874	27.539030537883104	27.38224877430075	22.584204386360277
60-64	22.52854079089999	27.572849123021236	27.38515537694554	22.51345470913323
65-69	22.460860524373626	27.552045605409536	27.446864418598665	22.540229451618174
70-74	22.426419437845333	27.61502119796801	27.40032286274024	22.55823650144642
75-79	22.46999692939218	27.53450782586003	27.434481429406848	22.56101381534095
80-84	22.434899822168113	27.592821840700697	27.407110522129024	22.565167815002162
85-89	22.41362703427768	27.564936172657944	27.418085700484653	22.603351092579725
90-94	21.515904188158355	27.296863891737686	29.129211606552996	22.058020313550962
95-99	22.45455596083167	27.56195511560737	27.51816200436728	22.465326919193682
100-104	22.558887010604643	27.673013774395532	27.31805884321952	22.450040371780304
105-109	22.418677001639683	27.672087398481487	27.500271677718104	22.408963922160726
110-114	22.453447983868063	27.436805277040357	27.56741730867057	22.542329430421017
115-119	22.465645095371947	27.556220307851824	27.411583595816857	22.56655100095937
120-124	22.375477006566676	27.59768067430834	27.48431438715096	22.54252793197402
125-129	22.369969030496424	27.59073080980024	27.529126468733566	22.510173690969772
130-134	22.43060515545786	27.610219290829	27.482255001684397	22.47692055202874
135-139	22.468081780508065	27.584977425660952	27.42780087171034	22.51913992212065
140-144	22.46945328345574	27.662761374981663	27.47207284485689	22.395712496705713
145-149	22.373203721392485	27.688531390358307	27.523059481948685	22.415205406300522
150-154	22.28424335725161	27.80238998951818	27.583434191065265	22.32993246216494
155-159	22.282584891545802	27.754228757385395	27.66834519650599	22.294841154562814
160-164	22.199825239058246	27.901209906743862	27.67174689493359	22.227217959264298
165-169	22.12261822045835	27.906312477269346	27.73093488939097	22.24013441288134
170-174	22.13798548272146	27.982878744985705	27.753300034919548	22.125835737373283
175-178	22.018801476722388	28.065466571497666	27.788836835975122	22.126895115804825
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	2.5
13	6.5
14	17.0
15	56.0
16	113.5
17	210.0
18	437.5
19	866.5
20	1682.0
21	3233.5
22	5898.5
23	9995.0
24	15973.0
25	24191.5
26	34984.5
27	48334.0
28	63858.0
29	81812.5
30	101646.5
31	123224.5
32	146788.0
33	172113.0
34	198694.0
35	225793.0
36	252868.0
37	278583.0
38	302634.0
39	324629.0
40	342231.0
41	355168.0
42	364308.5
43	367530.0
44	363813.5
45	355641.0
46	342699.5
47	324744.5
48	303466.0
49	280688.0
50	257164.5
51	232957.5
52	210757.5
53	190786.0
54	173103.5
55	157606.5
56	144593.5
57	133998.5
58	124769.5
59	116470.0
60	108346.5
61	100691.5
62	92890.5
63	84531.0
64	75561.0
65	66313.0
66	57139.0
67	48019.5
68	39678.5
69	32043.5
70	24853.5
71	18769.5
72	13982.5
73	10125.0
74	6984.0
75	4743.5
76	3162.0
77	2015.5
78	1272.5
79	751.5
80	431.0
81	248.5
82	125.0
83	63.5
84	37.0
85	19.0
86	9.0
87	3.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	700.0
92-93	1676.0
94-95	2645.0
96-97	3495.0
98-99	4373.0
100-101	5205.0
102-103	6238.0
104-105	6600.0
106-107	7343.0
108-109	8043.0
110-111	8498.0
112-113	8932.0
114-115	9166.0
116-117	9548.0
118-119	9846.0
120-121	10267.0
122-123	10526.0
124-125	11284.0
126-127	11678.0
128-129	12277.0
130-131	13170.0
132-133	14293.0
134-135	73220.0
136-137	76428.0
138-139	75082.0
140-141	76117.0
142-143	77264.0
144-145	80390.0
146-147	70780.0
148-149	67270.0
150-151	67338.0
152-153	66541.0
154-155	60448.0
156-157	60704.0
158-159	63192.0
160-161	65454.0
162-163	67453.0
164-165	68250.0
166-167	70503.0
168-169	74649.0
170-171	77273.0
172-173	81983.0
174-175	85170.0
176-177	182646.0
178-179	6489437.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.0549835699281
#Duplication Level	Percentage of total
1	90.61716485161152
2	8.101077316750702
3	0.9577937352270222
4	0.18009286691890225
5	0.05732013943669336
6	0.02986124486677153
7	0.018645234508373396
8	0.013546243127636809
9	0.006289290691519459
>10	0.018209076860866154
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.3942361159744034E-5	2.3942361159744034E-5	0.0	0.0	0.0
2	3.591354173961605E-5	2.3942361159744034E-5	2.3942361159744034E-5	0.0	0.0	0.0
3	3.591354173961605E-5	4.788472231948807E-5	2.3942361159744034E-5	2.3942361159744034E-5	0.0	0.0
4	3.591354173961605E-5	5.985590289936008E-5	2.3942361159744034E-5	4.788472231948807E-5	0.0	0.0
5	3.591354173961605E-5	7.18270834792321E-5	2.3942361159744034E-5	5.985590289936008E-5	0.0	0.0
6	3.591354173961605E-5	7.18270834792321E-5	3.591354173961605E-5	5.985590289936008E-5	0.0	0.0
7	3.591354173961605E-5	7.18270834792321E-5	3.591354173961605E-5	7.18270834792321E-5	1.1971180579872017E-5	0.0
8	3.591354173961605E-5	8.379826405910412E-5	3.591354173961605E-5	7.18270834792321E-5	1.1971180579872017E-5	0.0
9	7.18270834792321E-5	8.379826405910412E-5	3.591354173961605E-5	7.18270834792321E-5	1.1971180579872017E-5	0.0
10-14	8.379826405910412E-5	9.337520852300173E-5	3.591354173961605E-5	1.0534638910287375E-4	1.1971180579872017E-5	0.0
15-19	9.098097240702732E-5	1.1252909745079695E-4	5.746166678338568E-5	1.580195836543106E-4	1.1971180579872017E-5	0.0
20-24	1.1013486133482256E-4	1.1971180579872014E-4	7.661555571118091E-5	1.9632736150990105E-4	1.1971180579872017E-5	0.0
25-29	1.2928875026261777E-4	1.2210604191469456E-4	9.098097240702732E-5	2.6336597275718437E-4	1.1971180579872017E-5	0.0
30-34	2.4181784771341473E-4	1.4844263919041301E-4	1.0295215298689935E-4	3.160391673086212E-4	3.591354173961605E-5	2.3942361159744035E-6
35-39	7.517901404159625E-4	1.7238500035015705E-4	1.1013486133482256E-4	3.878662507878533E-4	6.703861124728329E-5	1.436541669584642E-5
40-44	0.0011516275717836878	2.0351006985782429E-4	1.4125993084248976E-4	4.788472231948807E-4	1.1252909745079696E-4	2.873083339169284E-5
45-49	0.0014293589612367188	2.274524310175683E-4	1.7238500035015705E-4	5.387031260942408E-4	1.436541669584642E-4	3.591354173961605E-5
50-54	0.001726244239617545	2.561832644092612E-4	1.987215976258755E-4	5.698281956019079E-4	1.6520229200223382E-4	3.591354173961605E-5
55-59	0.0019273600733593944	2.96885278380826E-4	2.274524310175683E-4	6.201071540373704E-4	2.0351006985782429E-4	3.591354173961605E-5
60-64	0.0020183410457664222	3.1125069507667244E-4	2.4181784771341473E-4	6.416552790811401E-4	2.4660631994536357E-4	4.0702013971564856E-5
65-69	0.0020853796570137048	3.1125069507667244E-4	2.8012562556900517E-4	6.655976402408842E-4	3.0885645896069804E-4	5.027895843546247E-5
70-74	0.00425216334197054	3.1125069507667244E-4	2.992795144968004E-4	7.134823625603722E-4	3.3040458400446763E-4	7.18270834792321E-5
75-79	0.04594778530166477	3.3040458400446763E-4	3.160391673086212E-4	7.78126737691681E-4	3.8307777855590454E-4	9.816368075495053E-5
80-84	0.06404581610231527	3.4716423681628843E-4	3.351930562364164E-4	8.236172238951947E-4	4.764529870789062E-4	1.1971180579872014E-4
85-89	0.06416792214422998	3.711065979760325E-4	3.543469451642117E-4	8.715019462146829E-4	6.512322235450377E-4	1.1971180579872014E-4
90-94	0.06420144144985362	3.8068354243993015E-4	3.7828930632395575E-4	9.145981963022221E-4	6.799630569367306E-4	1.1971180579872014E-4
95-99	0.06426608582498494	3.8547201467187893E-4	4.165970841795462E-4	9.6248291862171E-4	6.94328473632577E-4	1.1971180579872014E-4
100-104	0.06435227832516	4.0702013971564857E-4	4.33356736991367E-4	9.888195158974287E-4	7.086938903284233E-4	1.1971180579872014E-4
105-109	0.0644360765892191	4.1420284806357175E-4	4.668760426150086E-4	0.0010031849325932748	7.374247237201163E-4	1.1971180579872014E-4
110-114	0.06450550943658237	4.1899132029552053E-4	4.980011121226759E-4	0.001027127293753019	7.54184376531937E-4	1.1971180579872014E-4
115-119	0.064541422978322	4.285682647594182E-4	5.243377093983942E-4	0.001046281182680814	7.54184376531937E-4	1.1971180579872014E-4
120-124	0.06483591402058685	4.3575097310734144E-4	5.45885834442164E-4	0.0010869831966523792	7.733382654597323E-4	1.3647145861054097E-4
125-129	0.06538898256337691	4.45327917571239E-4	5.674339594859336E-4	0.0011205025022760207	7.900979182715531E-4	1.436541669584642E-4
130-134	0.06597557041179065	4.7166451484695744E-4	5.841936122977544E-4	0.0011731756968274575	8.140402794312971E-4	1.436541669584642E-4
135-139	0.06657173520466828	4.81241459310855E-4	5.865878484137288E-4	0.00128331055816228	8.619250017507851E-4	1.436541669584642E-4
140-144	0.06718705388647371	4.908184037747526E-4	5.961647928776263E-4	0.001324012572133845	8.882615990265036E-4	1.436541669584642E-4
145-149	0.06755576624833376	4.908184037747526E-4	6.320783346172424E-4	0.0013623203499894356	9.074154879542988E-4	1.5562534753833622E-4
150-154	0.06776167055430755	4.956068760067014E-4	6.655976402408842E-4	0.0014102050723089235	9.600886825057357E-4	1.5562534753833622E-4
155-159	0.06807052701326825	5.027895843546247E-4	6.991169458645258E-4	0.0014317531973526935	9.696656269696333E-4	1.5562534753833622E-4
160-164	0.0684967010419117	5.147607649344967E-4	7.230593070242698E-4	0.0014556955585124372	9.720598630856076E-4	1.5562534753833622E-4
165-167	0.06897395210769593	5.147607649344967E-4	7.50193983005313E-4	0.00148442639190413	9.856272010761293E-4	1.5562534753833622E-4
>>END_MODULE
