##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293754
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8835619
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.05770687939351	41.0	40.0	41.0	38.0	41.0
2	40.172742622786245	41.0	40.0	41.0	38.0	41.0
3	40.188145618320576	41.0	40.0	41.0	38.0	41.0
4	40.183563483214925	41.0	40.0	41.0	38.0	41.0
5	40.171115572095175	41.0	40.0	41.0	38.0	41.0
6	40.15882384697665	41.0	40.0	41.0	38.0	41.0
7	40.151664982385505	41.0	40.0	41.0	38.0	41.0
8	40.13082207369965	41.0	40.0	41.0	38.0	41.0
9	40.09991682529543	41.0	40.0	41.0	38.0	41.0
10-14	40.02781889984165	41.0	40.0	41.0	38.0	41.0
15-19	39.88223041305878	41.0	40.0	41.0	38.0	41.0
20-24	39.69740155160606	40.4	39.6	41.0	37.4	41.0
25-29	39.603108757858394	40.8	39.0	41.0	37.0	41.0
30-34	39.38536908393176	40.0	38.8	41.0	35.8	41.0
35-39	38.97431541581863	40.0	37.8	41.0	35.0	41.0
40-44	38.513630590001675	39.6	36.6	41.0	35.0	41.0
45-49	37.7517198512068	38.2	35.2	40.6	34.8	41.0
50-54	36.95139899083471	36.6	35.0	39.2	34.0	41.0
55-59	35.915460229781296	35.6	34.6	37.8	33.4	40.0
60-64	35.537669381171824	35.0	34.8	36.6	33.6	39.0
65-69	35.33142110360349	35.0	35.0	36.0	34.0	37.2
70-74	35.111605061286596	35.0	35.0	35.2	34.0	36.8
75-79	35.01078631842319	35.0	35.0	35.0	33.8	36.0
80-84	34.87432226310347	35.0	34.8	35.0	32.8	36.0
85-89	40.044097283959395	41.0	40.0	41.0	38.0	41.0
90-94	39.98807986052918	41.0	40.0	41.0	38.0	41.0
95-99	39.847127019111625	41.0	40.0	41.0	37.8	41.0
100-104	39.68782514983959	41.0	39.6	41.0	37.0	41.0
105-109	39.45983920290821	40.2	39.0	41.0	36.2	41.0
110-114	39.25308491328504	40.0	38.4	41.0	35.0	41.0
115-119	38.63695408371686	39.4	37.4	40.4	34.8	40.6
120-124	38.63038803119528	40.0	37.0	40.8	35.0	41.0
125-129	38.08236622085943	39.0	35.6	41.0	34.8	41.0
130-134	37.291692077744415	37.2	35.0	39.8	34.0	41.0
135-139	36.60087880578359	36.2	35.0	39.0	34.0	41.0
140-144	36.00574805846191	35.2	35.0	37.0	34.0	39.2
145-149	35.42295320001942	35.0	35.0	36.0	34.0	37.8
150-154	35.030544674825684	35.0	35.0	35.4	34.0	36.6
155-159	34.80252307935346	35.0	35.0	35.0	34.0	36.0
160-164	34.68086520510481	35.0	35.0	35.0	33.4	36.0
165-168	34.442412676719236	35.0	34.75	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	9.0
30	166.0
31	2179.0
32	14067.0
33	57009.0
34	167210.0
35	426423.0
36	1173588.0
37	2999121.0
38	3314833.0
39	676361.0
40	4653.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.62809419464556	27.691710111085595	29.124218688017216	21.555977006251627
2	21.588312035636665	28.020832496285774	28.643165804229447	21.747689663848114
3	21.695638981264356	28.200797250311492	28.19347461677558	21.91008915164857
4	21.931638292687815	28.29862853977746	28.192229655896206	21.577503511638515
5	21.673059918043094	28.515851577574814	28.090493716399497	21.7205947879826
6	21.79153492245422	28.507340572290406	27.95570972446865	21.745414780786724
7	22.239505800329326	28.355545887616927	27.91270198499958	21.492246327054165
8	21.845158782876446	28.393698279656466	27.78177737179478	21.97936556567231
9	21.817962046575346	28.395950527065505	27.850046499288844	21.936040927070305
10-14	21.74582222252906	28.483188331230668	28.23620167415548	21.534787772084783
15-19	21.613949175490703	28.18259592225514	28.508338804559134	21.69511609769502
20-24	21.671545592900735	28.19384584147415	28.240830665061495	21.893777900563617
25-29	21.75244994153777	28.284365815230377	28.236194883459774	21.726989359772077
30-34	21.633266441208026	28.290945999369143	28.274603058370896	21.801184501051935
35-39	21.63488036322073	28.297793284205667	28.262626534711377	21.804699817862222
40-44	21.76068931899395	28.210455883170155	28.222683662570784	21.806171135265114
45-49	21.811384126001812	28.284698559319953	28.140167655486277	21.76374965919196
50-54	21.79586512274918	28.200231359002693	28.13900644652061	21.864897071727516
55-59	21.782408227425833	28.283175179916654	28.183161813563938	21.751254779093575
60-64	21.76119635760664	28.23552486815015	28.18574454149732	21.81753423274589
65-69	21.706501830828152	28.261863713227108	28.166246190561182	21.865388265383558
70-74	21.73196467615908	28.24518576457405	28.239531378616483	21.783318180650387
75-79	21.68928515364911	28.271843772349172	28.18004035710458	21.858830716897142
80-84	21.66109471220975	28.22631215764283	28.209491604379956	21.903101525767465
85-89	21.640000547782787	28.217907539924482	28.43012809855201	21.711963813740724
90-94	21.82506816471129	28.453004647680903	27.956796382967315	21.765130804640496
95-99	21.58106967124312	28.469644835783775	28.314365782975347	21.634919709997753
100-104	21.607228482153932	28.150960133471326	28.492507213091773	21.74930417128297
105-109	21.644580286014833	28.29504172508488	28.203516451177475	21.856861537722818
110-114	21.627814969677566	28.272812549201703	28.221173907664408	21.87819857345632
115-119	21.620725419667146	28.28278376055059	28.30767621447881	21.788814605303454
120-124	21.59284081872065	28.33490287683169	28.287579335295177	21.784676969152482
125-129	21.666845578867147	28.259410929337285	28.154577657068714	21.91916583472685
130-134	21.764703997472488	28.30417785802397	28.18156555905822	21.749552585445322
135-139	21.717771587797188	28.32412832258349	28.16989961484802	21.788200474771298
140-144	21.62759456277265	28.379806753844385	28.230960643771947	21.76163803961102
145-149	21.647227612288432	28.376634750087447	28.289300110684806	21.68683752693931
150-154	21.58133095253656	28.4707209208233	28.279580921445756	21.66836720519438
155-159	21.536023151146402	28.423462869384224	28.325568790408962	21.71494518906041
160-164	21.536575868556326	28.53725271174411	28.34473973530738	21.581431684392186
165-168	21.46715150756206	28.55269807585175	28.320470877820487	21.6596795387657
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	1.5
10	8.0
11	20.0
12	40.5
13	66.5
14	155.5
15	308.0
16	575.0
17	1096.5
18	1867.5
19	3109.5
20	5229.0
21	8332.0
22	12815.5
23	19011.0
24	26931.0
25	36854.5
26	48786.5
27	62668.5
28	78462.0
29	96235.5
30	115856.5
31	137433.0
32	161042.5
33	186116.0
34	212694.0
35	240955.5
36	269413.0
37	297413.5
38	324773.5
39	350781.0
40	373588.0
41	391500.5
42	405137.5
43	413674.5
44	415490.0
45	410582.0
46	399393.0
47	382875.0
48	361542.0
49	335666.0
50	307807.5
51	279050.5
52	249417.5
53	220023.5
54	192531.0
55	166979.5
56	142578.5
57	121069.0
58	102366.0
59	85703.0
60	71640.0
61	60110.0
62	50387.0
63	41758.0
64	34334.5
65	28076.0
66	22581.0
67	18019.5
68	14270.0
69	10992.5
70	8375.0
71	6200.0
72	4438.0
73	3153.5
74	2155.0
75	1414.0
76	919.5
77	577.0
78	364.5
79	220.0
80	126.0
81	69.5
82	38.0
83	17.5
84	6.0
85	4.0
86	2.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	761.0
92-93	1922.0
94-95	2998.0
96-97	3917.0
98-99	4943.0
100-101	6022.0
102-103	7184.0
104-105	7052.0
106-107	7679.0
108-109	8122.0
110-111	8515.0
112-113	8976.0
114-115	9530.0
116-117	10171.0
118-119	10785.0
120-121	11470.0
122-123	11740.0
124-125	12169.0
126-127	12656.0
128-129	46906.0
130-131	93509.0
132-133	89038.0
134-135	85344.0
136-137	76281.0
138-139	72223.0
140-141	76827.0
142-143	77004.0
144-145	70662.0
146-147	70535.0
148-149	76758.0
150-151	71616.0
152-153	72746.0
154-155	75380.0
156-157	80382.0
158-159	80799.0
160-161	83453.0
162-163	85431.0
164-165	91082.0
166-167	218756.0
168-169	6994275.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.93423499248165
#Duplication Level	Percentage of total
1	80.30603816142697
2	14.129471628774992
3	3.4144280598734915
4	1.0671100497117962
5	0.39516172686956924
6	0.18791770531555743
7	0.11565984890950416
8	0.05088666058077394
9	0.044907087267918006
>10	0.28673135778733205
>50	0.0
>100	0.0016877134820959525
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.1317826176072102E-5	1.1317826176072102E-5	0.0
2	0.0	0.0	0.0	1.1317826176072102E-5	3.3953478528216305E-5	0.0
3	0.0	3.3953478528216305E-5	0.0	1.1317826176072102E-5	4.5271304704288406E-5	0.0
4	2.2635652352144203E-5	3.3953478528216305E-5	0.0	2.2635652352144203E-5	5.658913088036051E-5	0.0
5	3.3953478528216305E-5	3.3953478528216305E-5	2.2635652352144203E-5	3.3953478528216305E-5	6.790695705643261E-5	0.0
6	4.5271304704288406E-5	3.3953478528216305E-5	2.2635652352144203E-5	4.5271304704288406E-5	6.790695705643261E-5	0.0
7	4.5271304704288406E-5	3.3953478528216305E-5	2.2635652352144203E-5	4.5271304704288406E-5	6.790695705643261E-5	0.0
8	4.5271304704288406E-5	3.3953478528216305E-5	2.2635652352144203E-5	4.5271304704288406E-5	6.790695705643261E-5	0.0
9	4.5271304704288406E-5	3.3953478528216305E-5	2.2635652352144203E-5	5.658913088036051E-5	7.92247832325047E-5	0.0
10-14	6.564339182121818E-5	5.206200040993167E-5	3.3953478528216305E-5	7.243408752686146E-5	1.1770539223114983E-4	0.0
15-19	1.1770539223114983E-4	8.601547893814795E-5	5.658913088036051E-5	1.2675965317200753E-4	1.5392243599458058E-4	0.0
20-24	1.471317402889373E-4	1.335503488776508E-4	6.790695705643261E-5	1.584495664650094E-4	1.8334878405236804E-4	0.0
25-29	1.675038274058671E-4	1.5165887075936613E-4	8.148834846771912E-5	2.1503869734536995E-4	2.7841852393137366E-4	0.0
30-34	1.924030449932257E-4	1.584495664650094E-4	1.0186043558464892E-4	2.5578287157922944E-4	3.6896113333995047E-4	2.2635652352144205E-6
35-39	2.897363501074458E-4	1.8108521881715362E-4	1.1996895746636428E-4	3.0784487198916115E-4	4.6403087321895617E-4	3.168991329300188E-5
40-44	3.6443400286952164E-4	2.2635652352144203E-4	1.3581391411286522E-4	3.282169591060909E-4	6.473796572713242E-4	3.3953478528216305E-5
45-49	4.391316556315975E-4	2.3767434969751414E-4	1.7203095787629593E-4	3.734882638103793E-4	9.529609640252708E-4	4.074417423385956E-5
50-54	5.206200040993165E-4	2.444650454031574E-4	1.8108521881715362E-4	4.210231337498821E-4	0.001097829139078994	6.111626135078935E-5
55-59	5.953176568613926E-4	2.557828715792295E-4	1.8561234928758246E-4	4.595037427485273E-4	0.0012472244446031454	8.601547893814797E-5
60-64	6.903873967403981E-4	2.69364262990516E-4	2.0824800163972667E-4	4.776122646302426E-4	0.0013332399235412933	9.054260940857681E-5
65-69	0.001072929921491635	2.897363501074458E-4	2.2862008875665645E-4	5.138293083936734E-4	0.0015369607947105911	9.506973987900566E-5
70-74	0.008366137109352497	3.2368982863566204E-4	2.6031000204965833E-4	5.523099173923185E-4	0.0016388212302952403	1.1317826176072102E-4
75-79	0.011446849394479323	3.8254252475123697E-4	2.738913934609449E-4	5.975812220966069E-4	0.001804061492465893	1.1317826176072102E-4
80-84	0.011498911394889256	4.52713047042884E-4	2.85209219637017E-4	6.066354830374647E-4	0.0021481234082184847	1.1317826176072102E-4
85-89	0.011564554786710474	4.7308513415981383E-4	3.055813067539467E-4	6.2474400491918E-4	0.00220697610433406	1.1317826176072102E-4
90-94	0.011587190439062617	4.934572212767437E-4	3.0784487198916115E-4	6.609610486826107E-4	0.002247720278567919	1.1317826176072102E-4
95-99	0.011636988874237333	5.138293083936735E-4	3.485890462230207E-4	6.994416576812559E-4	0.002295255148507422	1.1317826176072102E-4
100-104	0.011668678787530336	5.455192216866753E-4	3.93860350927309E-4	7.220773100334E-4	0.0023450535836821392	1.1317826176072102E-4
105-109	0.01173658574458677	5.839998306853203E-4	4.029146118681668E-4	7.922478323250471E-4	0.00238127062744557	1.1317826176072102E-4
110-114	0.011775066353585415	6.066354830374646E-4	4.1649600327945335E-4	8.78263311263195E-4	0.002460495410678075	1.1317826176072102E-4
115-119	0.011842973310641845	6.202168744487511E-4	4.187595685146678E-4	9.348524421435555E-4	0.002614417846672655	1.1317826176072102E-4
120-124	0.01196294226810821	6.292711353896089E-4	4.210231337498821E-4	9.959687034943449E-4	0.0027434410650798774	1.244960879367931E-4
125-129	0.012089701921280218	6.586974834473962E-4	4.300773946907399E-4	0.0010276586167873465	0.0029562161971900323	1.3355034887765077E-4
130-134	0.012225515835393084	6.677517443882539E-4	4.459223513372408E-4	0.0011068834000198514	0.003164464198829759	1.5165887075936613E-4
135-139	0.012322849140507302	6.813331357995405E-4	4.7082156892459934E-4	0.0011340461828424245	0.003295750982472196	1.584495664650094E-4
140-144	0.01237717470615245	6.903873967403981E-4	4.979843517471724E-4	0.0011498911394889253	0.003497208288406279	1.584495664650094E-4
145-149	0.012524306446441386	7.062323533868991E-4	5.206200040993167E-4	0.0011815810527819274	0.0036850842029290767	1.6297669693543824E-4
150-154	0.012644275403907751	7.356587014446866E-4	5.38728525981032E-4	0.0012064802703692858	0.0039001229002744464	1.6976739264108152E-4
155-157	0.012774053144060044	7.545217450714735E-4	5.545734826275329E-4	0.0012185526182904296	0.004074417423385956	1.7731261009179623E-4
>>END_MODULE
