##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293757
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8743903
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.07294397021559	41.0	40.0	41.0	38.0	41.0
2	40.17709940286392	41.0	40.0	41.0	38.0	41.0
3	40.1856248862779	41.0	40.0	41.0	38.0	41.0
4	40.18379412488908	41.0	40.0	41.0	38.0	41.0
5	40.17275214512329	41.0	40.0	41.0	38.0	41.0
6	40.15739584485326	41.0	40.0	41.0	38.0	41.0
7	40.15209786750837	41.0	40.0	41.0	38.0	41.0
8	40.12849216190985	41.0	40.0	41.0	38.0	41.0
9	40.105234470236006	41.0	40.0	41.0	38.0	41.0
10-14	40.02863421517828	41.0	40.0	41.0	38.0	41.0
15-19	39.88567231361098	41.0	40.0	41.0	38.0	41.0
20-24	39.698276822146816	40.4	39.6	41.0	37.4	41.0
25-29	39.603752603385466	40.6	39.0	41.0	37.0	41.0
30-34	39.3811206734567	40.0	38.8	41.0	35.8	41.0
35-39	38.96946784519453	40.0	37.8	41.0	35.0	41.0
40-44	38.50374856628671	39.4	36.6	41.0	35.0	41.0
45-49	37.737101864007414	38.2	35.2	40.6	34.8	41.0
50-54	36.929610724181174	36.6	35.0	39.2	34.0	41.0
55-59	35.919882780035415	35.4	34.6	37.4	33.4	40.0
60-64	35.52887917443732	35.0	34.8	36.6	33.6	39.0
65-69	35.31386331710222	35.0	35.0	36.0	34.0	37.2
70-74	35.092195213053024	35.0	35.0	35.2	34.0	36.6
75-79	34.988454309248404	35.0	35.0	35.0	33.8	36.0
80-84	34.852018783831426	35.0	34.8	35.0	32.8	36.0
85-89	40.04649873174485	41.0	40.0	41.0	38.0	41.0
90-94	39.9911761044235	41.0	40.0	41.0	38.0	41.0
95-99	39.84199149043173	41.0	40.0	41.0	37.8	41.0
100-104	39.68642362211206	41.0	39.6	41.0	37.0	41.0
105-109	39.46256490068111	40.2	39.0	41.0	36.2	41.0
110-114	39.254896845789496	40.0	38.6	41.0	35.0	41.0
115-119	38.65934052966753	39.4	37.4	40.4	34.8	40.8
120-124	38.62762889462848	40.0	37.0	41.0	35.0	41.0
125-129	38.06275089236522	39.0	35.6	41.0	34.8	41.0
130-134	37.2673574681087	37.2	35.0	39.8	34.0	41.0
135-139	36.573433523929	36.2	35.0	39.0	34.0	41.0
140-144	35.97571109856206	35.2	35.0	37.0	34.0	39.2
145-149	35.4034178517674	35.0	35.0	36.0	34.0	37.6
150-154	35.01243107393322	35.0	35.0	35.4	34.0	36.4
155-159	34.791434221745725	35.0	35.0	35.0	34.0	36.0
160-164	34.66888188057001	35.0	35.0	35.0	33.4	36.0
165-168	34.43956185436423	35.0	34.75	35.0	32.5	35.75
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	189.0
31	2077.0
32	13904.0
33	55933.0
34	166026.0
35	424896.0
36	1174892.0
37	3000225.0
38	3261931.0
39	640494.0
40	3330.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.62559442848348	27.683335462435938	29.08225308537846	21.608817023702116
2	21.591261934172877	28.012067379978937	28.64884251346338	21.747828172384803
3	21.742452998392135	28.14612650666413	28.178011581327013	21.933408913616724
4	21.949271395165294	28.286978938352814	28.18297503986492	21.580774626616968
5	21.724623431893058	28.48473959512131	28.059460403437686	21.731176569547948
6	21.796124682535932	28.483904727671383	27.93624311706111	21.78372747273157
7	22.234487276448515	28.398279349622246	27.860796259976812	21.50643711395243
8	21.869684510452597	28.385230257014516	27.71296753863807	22.032117693894822
9	21.86102704936228	28.374136812816886	27.78769389367654	21.97714224414429
10-14	21.764747390267253	28.476320014071522	28.20117057565712	21.55776202000411
15-19	21.633501652522906	28.154635292729118	28.492422662968696	21.719440391779276
20-24	21.69054254147147	28.172984078162806	28.226520811129767	21.90995256923596
25-29	21.74452072489825	28.267543681580182	28.218805720969232	21.769129872552337
30-34	21.654965751564262	28.265944853230874	28.254931464816114	21.82415793038875
35-39	21.649487648707904	28.282445493734322	28.236100057377122	21.831966800180652
40-44	21.781597988907244	28.224697826588425	28.185843324199734	21.807860860304604
45-49	21.827314415541892	28.255279135644574	28.11770212912929	21.799704319684242
50-54	21.811280385887173	28.18411411928975	28.11976070640308	21.88484478842
55-59	21.786275533934905	28.272905131724357	28.157412084740645	21.783407249600092
60-64	21.779182591572667	28.223098998239116	28.153466478299222	21.844251931888998
65-69	21.724657741514285	28.246006388680208	28.144948543001906	21.884387326803605
70-74	21.747585717728114	28.21315149539056	28.224313558830648	21.81494922805068
75-79	21.714991577559815	28.264435229896762	28.14889186213525	21.871681330408173
80-84	21.67491336534726	28.210001872161666	28.178544524110116	21.93654023838096
85-89	21.65762360355553	28.189775206792667	28.41800280721321	21.734598382438598
90-94	21.848772925185607	28.448257561449818	27.93291441386045	21.770055099504127
95-99	21.59448233739711	28.452867542468326	28.28659869171161	21.666051428422954
100-104	21.631070475159323	28.13254921032956	28.47636866214766	21.760011652363453
105-109	21.682637180079116	28.259081599863013	28.178036323643514	21.880244896414357
110-114	21.649238712257873	28.248876602919417	28.200192379793478	21.90169230502924
115-119	21.633380144854545	28.268816924133876	28.283804057562133	21.813998873449446
120-124	21.62042505581915	28.304791020383163	28.26236520243161	21.812418721366072
125-129	21.688408951725226	28.241865885399246	28.129768187581476	21.939956975294052
130-134	21.791824966674874	28.306378148105466	28.14091377317115	21.76088311204851
135-139	21.737506240374262	28.319015325842127	28.127448943699317	21.816029490084286
140-144	21.65206064497965	28.37009040022486	28.21111419522715	21.766734759568344
145-149	21.67867482736239	28.336910722818132	28.271011664272205	21.713402785547274
150-154	21.616150351770287	28.462863984443494	28.232119313479647	21.688866350306572
155-159	21.569308955973305	28.410077175901915	28.292246651623724	21.728367216501052
160-164	21.575332672682485	28.488446197715255	28.32213495237828	21.614086177223975
165-168	21.481925805886107	28.521341970608326	28.325139077965584	21.671593145539987
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	1.5
10	4.5
11	15.0
12	45.0
13	93.0
14	163.0
15	307.5
16	571.0
17	1020.5
18	1804.0
19	3130.5
20	5200.0
21	8257.5
22	12687.0
23	18572.5
24	25902.5
25	35795.0
26	47786.5
27	61605.0
28	77346.5
29	94892.5
30	114225.0
31	135018.5
32	157886.0
33	182725.0
34	209168.5
35	237044.5
36	265554.5
37	294328.0
38	321435.0
39	346229.5
40	369281.0
41	387486.0
42	400551.0
43	408725.5
44	410769.0
45	406018.5
46	394947.5
47	378445.5
48	356814.5
49	332399.0
50	305665.0
51	277209.0
52	248421.0
53	219574.5
54	191698.0
55	165739.0
56	142123.0
57	120984.5
58	102159.0
59	85760.5
60	71747.5
61	60138.5
62	50407.5
63	41672.5
64	34291.0
65	28194.0
66	22828.0
67	18217.0
68	14343.5
69	11047.0
70	8337.5
71	6075.5
72	4430.5
73	3173.0
74	2186.0
75	1469.5
76	939.0
77	589.5
78	378.5
79	203.0
80	104.0
81	68.0
82	30.0
83	9.0
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	765.0
92-93	1833.0
94-95	2878.0
96-97	3729.0
98-99	4443.0
100-101	5332.0
102-103	6014.0
104-105	6661.0
106-107	7421.0
108-109	7858.0
110-111	8214.0
112-113	8552.0
114-115	9274.0
116-117	9611.0
118-119	10081.0
120-121	10568.0
122-123	11193.0
124-125	11888.0
126-127	12319.0
128-129	46147.0
130-131	81134.0
132-133	82721.0
134-135	83210.0
136-137	71677.0
138-139	68980.0
140-141	74677.0
142-143	73167.0
144-145	67739.0
146-147	66205.0
148-149	68429.0
150-151	69677.0
152-153	70594.0
154-155	73309.0
156-157	75874.0
158-159	79218.0
160-161	80051.0
162-163	83144.0
164-165	87552.0
166-167	184334.0
168-169	7017430.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.97327421254829
#Duplication Level	Percentage of total
1	80.41691320002347
2	13.963326356594996
3	3.403645372722731
4	1.1150020045604951
5	0.4166931999467682
6	0.17358855303950174
7	0.10640080606896553
8	0.06752888080341299
9	0.05642007310575416
>10	0.2788091444485077
>50	0.0016724086853703646
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.1436540409929066E-5	1.1436540409929066E-5	1.1436540409929066E-5	1.1436540409929066E-5	2.2873080819858133E-5	0.0
2	1.1436540409929066E-5	2.2873080819858133E-5	1.1436540409929066E-5	1.1436540409929066E-5	4.5746161639716266E-5	0.0
3	1.1436540409929066E-5	3.43096212297872E-5	1.1436540409929066E-5	1.1436540409929066E-5	6.86192424595744E-5	0.0
4	2.2873080819858133E-5	4.5746161639716266E-5	1.1436540409929066E-5	1.1436540409929066E-5	8.005578286950347E-5	0.0
5	3.43096212297872E-5	4.5746161639716266E-5	2.2873080819858133E-5	2.2873080819858133E-5	8.005578286950347E-5	0.0
6	4.5746161639716266E-5	4.5746161639716266E-5	2.2873080819858133E-5	2.2873080819858133E-5	8.005578286950347E-5	0.0
7	4.5746161639716266E-5	5.7182702049645336E-5	3.43096212297872E-5	3.43096212297872E-5	8.005578286950347E-5	0.0
8	4.5746161639716266E-5	6.86192424595744E-5	4.5746161639716266E-5	3.43096212297872E-5	8.005578286950347E-5	0.0
9	5.7182702049645336E-5	8.005578286950347E-5	5.7182702049645336E-5	5.7182702049645336E-5	9.149232327943253E-5	0.0
10-14	8.234309095148928E-5	9.606693944340416E-5	5.718270204964534E-5	9.606693944340417E-5	1.1436540409929066E-4	0.0
15-19	1.1436540409929066E-4	1.3266386875517719E-4	7.548116670553186E-5	1.6468618190297856E-4	1.509623334110637E-4	0.0
20-24	1.395257930011346E-4	1.6239887382099274E-4	9.606693944340418E-5	2.127196516246806E-4	1.8984657080482254E-4	0.0
25-29	1.7154810614893601E-4	1.7383541423092184E-4	1.0292886368936161E-4	3.270850557239713E-4	3.225104395599997E-4	0.0
30-34	2.2186888395262392E-4	1.8298464655886506E-4	1.1436540409929066E-4	4.094281466754606E-4	4.8490931338099244E-4	1.1436540409929066E-5
35-39	3.636819850357443E-4	1.852719546408509E-4	1.372384849191488E-4	4.460250759872336E-4	6.564574195299284E-4	5.032077780368789E-5
40-44	4.3687584365929036E-4	1.9442118696879414E-4	1.555369495750353E-4	5.077823942008506E-4	8.280055256788644E-4	9.149232327943255E-5
45-49	5.649650962504958E-4	2.1043234354269484E-4	1.8755926272283672E-4	5.878381770703541E-4	0.0011116317278451054	1.0521617177134742E-4
50-54	6.724685761038292E-4	2.561785051824111E-4	2.0585772737872322E-4	6.427335710380136E-4	0.0012488702127642541	1.1894002026326231E-4
55-59	7.250766619895028E-4	2.6304042942836855E-4	2.355927324445388E-4	6.747558841858149E-4	0.0014890375613727646	1.2580194450921973E-4
60-64	7.502370508913468E-4	2.6532773751035437E-4	2.607531213463827E-4	6.861924245957441E-4	0.0016674475917676577	1.2580194450921973E-4
65-69	0.0011322175005829777	2.744769698382976E-4	2.8133889408425505E-4	7.250766619895028E-4	0.0018229845413426933	1.3266386875517719E-4
70-74	0.008748953413595736	2.973500506581558E-4	3.2479774764198547E-4	7.639608993832617E-4	0.0019533611020158847	1.372384849191488E-4
75-79	0.01205640090014722	3.796931416096451E-4	3.4309621229787203E-4	7.822593640391482E-4	0.0021134726677548917	1.372384849191488E-4
80-84	0.012111296294114882	4.1171545475744643E-4	3.4309621229787203E-4	8.234309095148929E-4	0.002468005420462692	1.372384849191488E-4
85-89	0.012152467839590628	4.3687584365929036E-4	3.476708284618437E-4	8.531659145807084E-4	0.0024977404255285087	1.372384849191488E-4
90-94	0.012207363233558286	4.734727729710634E-4	3.7511852544567343E-4	9.172105408763112E-4	0.0025046023497744658	1.372384849191488E-4
95-99	0.012237098238624101	5.192189346107796E-4	3.888423739375883E-4	9.789678590899282E-4	0.0025183261982663803	1.372384849191488E-4
100-104	0.01227140785985389	5.375173992666661E-4	4.002789143475174E-4	9.858297833358857E-4	0.0025526358194961677	1.372384849191488E-4
105-109	0.012282844400263818	5.443793235126236E-4	4.323012274953187E-4	0.0010292886368936161	0.0026441281427756003	1.4867502532907786E-4
110-114	0.012289706324509774	5.855508689883682E-4	4.3916315174127613E-4	0.0011207809601730485	0.0027653554711208487	1.6011156573900694E-4
115-119	0.012408646344773037	6.061366417262406E-4	4.5746161639716274E-4	0.0011756763541407081	0.0028865827994660966	1.7383541423092184E-4
120-124	0.012555034062020132	6.221477983001412E-4	4.5746161639716274E-4	0.0012282844400263817	0.003028395900549217	1.8298464655886506E-4
125-129	0.01274716794090694	6.312970306280846E-4	4.963458537909216E-4	0.0012831798339940415	0.003211380547108082	1.852719546408509E-4
130-134	0.012831798339940415	6.404462629560277E-4	5.192189346107797E-4	0.0013472244602896442	0.0034241001987327623	2.035704192967374E-4
135-139	0.012937014511711762	6.473081872019852E-4	5.352300911846803E-4	0.0013815340815194314	0.0036025102291276563	2.0585772737872322E-4
140-144	0.013017070294581264	6.518828033659569E-4	5.489539396765952E-4	0.0014524406320609916	0.003833528345408223	2.1729426778865227E-4
145-149	0.013170319936074315	6.770431922678007E-4	5.626777881685101E-4	0.0015050487179466652	0.004064546461688791	2.1729426778865227E-4
150-154	0.01329840918866552	6.953416569236873E-4	6.061366417262406E-4	0.0015782425765702112	0.00421779610318184	2.218688839526239E-4
155-157	0.013437934981666655	7.205020458255312E-4	6.061366417262406E-4	0.0016239887382099277	0.004334448815363116	2.2873080819858134E-4
>>END_MODULE
