##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293766
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8690800
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.39638744419386	39.0	39.0	39.0	37.0	39.0
2	40.18620161550145	41.0	40.0	41.0	38.0	41.0
3	40.22245017719888	41.0	40.0	41.0	38.0	41.0
4	40.23229127353063	41.0	40.0	41.0	38.0	41.0
5	40.230558981911905	41.0	40.0	41.0	38.0	41.0
6	40.220472683757535	41.0	40.0	41.0	38.0	41.0
7	40.24111359138399	41.0	40.0	41.0	38.0	41.0
8	40.22320729967322	41.0	40.0	41.0	38.0	41.0
9	40.20970301928476	41.0	40.0	41.0	38.0	41.0
10-14	40.153321075159944	41.0	40.0	41.0	38.0	41.0
15-19	40.03688056335435	41.0	40.0	41.0	38.0	41.0
20-24	39.868589542964976	41.0	40.0	41.0	37.8	41.0
25-29	39.6298073595066	40.8	39.2	41.0	36.8	41.0
30-34	39.395046232797895	40.0	39.0	41.0	35.6	41.0
35-39	39.21259080867124	40.0	38.2	41.0	35.0	41.0
40-44	38.708694757674785	39.8	36.6	41.0	35.0	41.0
45-49	38.001966217149175	38.8	35.2	41.0	35.0	41.0
50-54	37.249165255212404	37.0	35.0	40.0	34.6	41.0
55-59	36.52903806323929	35.8	35.0	38.8	34.0	41.0
60-64	35.77588528098679	35.0	34.8	36.8	33.6	39.4
65-69	35.44017828048051	35.0	35.0	36.0	33.8	38.8
70-74	35.26054595664381	35.0	35.0	35.8	34.0	37.0
75-79	35.11800359000323	35.0	35.0	35.0	34.0	36.8
80-84	35.05489347355824	35.0	35.0	35.0	33.6	36.0
85-89	34.87340631472362	35.0	34.6	35.0	32.6	36.2
90-94	39.64342312727845	40.6	39.8	40.6	37.8	40.6
95-99	39.93590073951857	41.0	40.0	41.0	38.0	41.0
100-104	39.82257784865628	41.0	40.0	41.0	37.2	41.0
105-109	39.6732269306535	41.0	39.8	41.0	36.6	41.0
110-114	39.492481039805085	41.0	39.0	41.0	35.8	41.0
115-119	39.2102058898107	40.0	38.4	41.0	35.0	41.0
120-124	38.93478635863454	40.0	38.0	41.0	35.0	41.0
125-129	38.38037986016632	39.6	36.6	40.6	34.6	41.0
130-134	38.06679673886822	39.0	35.4	41.0	34.0	41.0
135-139	37.66272937837503	38.2	35.0	41.0	34.4	41.0
140-144	37.035470867955006	36.6	35.0	39.6	34.0	41.0
145-149	36.33368900876923	35.4	35.0	38.2	34.0	40.8
150-154	35.70907996035604	35.0	35.0	36.6	34.0	39.0
155-159	35.234623787854396	35.0	35.0	36.0	34.0	37.4
160-164	34.91366109572853	35.0	35.0	35.0	33.6	36.2
165-169	34.71591713108918	35.0	35.0	35.0	33.0	36.0
170-174	34.613341430797476	35.0	35.0	35.0	33.0	36.0
175-178	34.31942739041525	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	56.0
30	531.0
31	3856.0
32	19528.0
33	70641.0
34	196802.0
35	475328.0
36	1265117.0
37	2846564.0
38	3051344.0
39	751147.0
40	9884.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.562917107746124	27.27944493027109	27.44707046531965	22.71056749666314
2	22.83574584618217	27.78306945275464	27.785209646983017	21.595975054080178
3	21.476273760758502	26.80551847931146	28.729449532839325	22.988758227090717
4	21.88241588806554	26.181456252588948	29.20515947898928	22.730968380356238
5	22.721958853039997	26.690592350531595	27.904070971602152	22.683377824826252
6	22.99268191650941	26.67008790905325	27.34954204446081	22.987688129976526
7	22.869747319004006	27.0544944078796	27.210118746260413	22.865639526855986
8	23.008549270492935	27.063423390251764	27.104305702582042	22.823721636673262
9	23.276372715975516	26.95582685138307	26.760608919777233	23.00719151286418
10-14	23.12645556220371	27.162484466332216	26.83049891839647	22.88056105306761
15-19	23.073700925116214	27.147213144934874	26.929461039259905	22.849624890689004
20-24	23.001578680903943	27.029504763658117	27.061920651723664	22.90699590371427
25-29	22.953214893910804	27.14896902471579	26.952193123763056	22.945622957610347
30-34	22.970405486261335	27.203412804344822	26.92958300731808	22.896598702075757
35-39	22.96364891609518	27.16740461177337	26.976826068946476	22.892120403184975
40-44	22.95166843098449	27.243823353431214	26.984869056933768	22.819639158650524
45-49	23.093549500621346	27.142207851981404	26.852793758917475	22.91144888847977
50-54	23.035568647305197	27.19706126018318	26.908123532931377	22.859246559580246
55-59	22.99616606066185	27.1774428130897	26.93045519399825	22.895935932250193
60-64	22.98733143093846	27.199783679293045	26.940074561605375	22.872810328163116
65-69	22.966912136972432	27.15459796566484	26.9808233994569	22.89766649790583
70-74	22.912804344824412	27.255792332121327	26.95309062456851	22.878312698485757
75-79	22.952036636443136	27.229062917107743	26.9124269342293	22.90647351221982
80-84	22.95337598379896	27.257120173056563	26.893551801905463	22.895952041239013
85-89	22.9490955953422	27.265466930547245	26.86066415059603	22.924773323514522
90-94	22.225534527785705	26.89291703299073	28.19502444955642	22.68652398966714
95-99	22.870561276124253	26.989681736297054	27.142487992518895	22.997268995059798
100-104	22.948036162319735	27.055997050309482	26.99793078656414	22.998036000806643
105-109	22.82326321072839	27.1155184952695	27.160747695711223	22.900470598290884
110-114	22.842602334740985	26.96559810390856	27.156824658356815	23.034974902993643
115-119	22.82650459218734	27.02500702347683	27.082973699809266	23.065514684526562
120-124	22.752684191429825	27.084819277998445	27.154767720885843	23.007728809685883
125-129	22.726829591939186	27.10809422289131	27.146906942594118	23.018169242575386
130-134	22.820053529800592	27.080576396930116	27.09066798779342	23.008702085475868
135-139	22.85902325230606	27.093196127642216	27.056382477154067	22.99139814289766
140-144	22.8474478379145	27.11419236630218	27.107694043607882	22.930665752175436
145-149	22.81360584812921	27.12132636852723	27.121802981686884	22.943264801656678
150-154	22.693262979721574	27.259570321183872	27.204806292730115	22.84236040636444
155-159	22.70547654750751	27.24171330770085	27.19645850817809	22.856351636613553
160-164	22.65960514460671	27.35110091178152	27.21640145325621	22.77289249035556
165-169	22.588548826801773	27.40104439759723	27.260386853897607	22.750019921703394
170-174	22.585600938888327	27.523190118317153	27.216346466140827	22.674862476653693
175-178	22.57248309473119	27.53510407461025	27.222687450258963	22.66972538039959
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	4.0
11	10.0
12	22.0
13	50.5
14	96.0
15	215.0
16	419.5
17	767.0
18	1426.0
19	2583.5
20	4452.5
21	7164.5
22	11225.0
23	16825.0
24	23873.5
25	32581.5
26	42961.5
27	54624.5
28	67709.5
29	82347.5
30	98106.0
31	115466.5
32	133590.0
33	152479.5
34	172262.0
35	192978.5
36	214849.5
37	236793.5
38	258052.5
39	278790.0
40	298117.5
41	315347.0
42	329595.5
43	340480.5
44	347264.0
45	350159.0
46	349417.5
47	344412.0
48	336466.0
49	324422.0
50	309584.5
51	293773.5
52	275892.0
53	256528.0
54	237664.5
55	219206.0
56	200771.5
57	182485.5
58	165207.0
59	148758.0
60	133291.5
61	119008.0
62	105111.5
63	92248.5
64	80142.0
65	68568.0
66	57885.5
67	48054.0
68	39032.0
69	31058.5
70	24237.0
71	18509.0
72	13921.5
73	10195.0
74	7230.5
75	5043.0
76	3420.5
77	2263.0
78	1512.5
79	1012.0
80	621.0
81	358.0
82	203.0
83	124.0
84	71.5
85	30.0
86	15.0
87	7.5
88	4.5
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1003.0
92-93	2239.0
94-95	3317.0
96-97	4781.0
98-99	5121.0
100-101	6120.0
102-103	6960.0
104-105	8098.0
106-107	9151.0
108-109	11081.0
110-111	11818.0
112-113	11425.0
114-115	13100.0
116-117	13759.0
118-119	13702.0
120-121	13388.0
122-123	14393.0
124-125	17909.0
126-127	15594.0
128-129	16146.0
130-131	16690.0
132-133	19221.0
134-135	86109.0
136-137	89579.0
138-139	79914.0
140-141	76456.0
142-143	73816.0
144-145	78198.0
146-147	80684.0
148-149	86190.0
150-151	85693.0
152-153	97982.0
154-155	76314.0
156-157	75744.0
158-159	104991.0
160-161	79824.0
162-163	80586.0
164-165	84548.0
166-167	86850.0
168-169	92559.0
170-171	86875.0
172-173	95209.0
174-175	97577.0
176-177	260631.0
178-179	6399455.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.1743317060116
#Duplication Level	Percentage of total
1	90.82250830639624
2	7.960846944755426
3	0.918971485198291
4	0.18404156960544502
5	0.05215571121672504
6	0.02674203960654823
7	0.013447735476990715
8	0.009363281556480632
9	0.005231551304714574
>10	0.005291363086326709
>50	0.0014000117968286604
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.451926174805541E-5	1.1506420582685139E-5	1.1506420582685139E-5	0.0	0.0	0.0
2	1.495834675749068E-4	1.1506420582685139E-5	1.1506420582685139E-5	3.451926174805541E-5	0.0	0.0
3	1.495834675749068E-4	2.3012841165370277E-5	2.3012841165370277E-5	3.451926174805541E-5	0.0	0.0
4	1.8410272932296222E-4	3.451926174805541E-5	3.451926174805541E-5	3.451926174805541E-5	0.0	0.0
5	1.9560914990564735E-4	3.451926174805541E-5	3.451926174805541E-5	5.753210291342569E-5	0.0	0.0
6	2.3012841165370276E-4	3.451926174805541E-5	3.451926174805541E-5	5.753210291342569E-5	0.0	0.0
7	2.3012841165370276E-4	4.6025682330740555E-5	3.451926174805541E-5	8.054494407879597E-5	0.0	0.0
8	2.4163483223638792E-4	4.6025682330740555E-5	3.451926174805541E-5	8.054494407879597E-5	0.0	0.0
9	2.5314125281907304E-4	4.6025682330740555E-5	3.451926174805541E-5	1.0355778524416625E-4	1.1506420582685139E-5	0.0
10-14	3.014682192663507E-4	5.753210291342569E-5	4.6025682330740555E-5	1.081603534772403E-4	1.1506420582685139E-5	0.0
15-19	3.405900492474801E-4	6.67372393795738E-5	5.292953468035164E-5	1.3577576287568464E-4	1.1506420582685139E-5	0.0
20-24	3.6590417452938743E-4	1.1506420582685138E-4	7.133980761264786E-5	1.5418603580798085E-4	2.5314125281907304E-5	0.0
25-29	4.0962857274359093E-4	1.4728218345836977E-4	1.1506420582685138E-4	1.818014452064252E-4	6.443595526303678E-5	0.0
30-34	5.223914944539052E-4	1.933078657891103E-4	1.426796152252957E-4	2.0481428637179547E-4	1.3577576287568464E-4	0.0
35-39	9.711418971786257E-4	2.2782712753716575E-4	1.67993740507203E-4	2.5314125281907304E-4	2.3933354811985088E-4	0.0
40-44	0.0014267961522529571	2.807566622175173E-4	2.0941685460486949E-4	2.669489575182952E-4	3.3828876513094304E-4	2.0711557048833248E-5
45-49	0.0019123671008422702	3.1757720808210985E-4	2.3012841165370276E-4	2.761540939844433E-4	4.1192985686012794E-4	3.451926174805541E-5
50-54	0.0024600727205780827	3.33686196897869E-4	2.3012841165370276E-4	3.129746398490358E-4	4.694619597735537E-4	3.912182998112947E-5
55-59	0.003173470796704561	3.659041745293874E-4	2.715515257513693E-4	3.6590417452938743E-4	5.338979150365903E-4	4.8326966447277584E-5
60-64	0.0037119712799742258	4.0962857274359093E-4	3.129746398490358E-4	3.705067427624615E-4	5.960325861830903E-4	5.753210291342569E-5
65-69	0.003999631794541355	4.3724398214203526E-4	3.33686196897869E-4	4.0502600451051686E-4	6.742762461453491E-4	6.443595526303678E-5
70-74	0.008491738390021633	4.3724398214203526E-4	3.590003221797763E-4	4.5105168684125743E-4	7.755327472729783E-4	6.903852349611083E-5
75-79	0.08956367653150459	4.487504027247204E-4	4.0502600451051686E-4	5.062825056381461E-4	8.30763566069867E-4	6.903852349611083E-5
80-84	0.12256178947852903	4.7406452800662774E-4	4.2573756155935016E-4	5.431030515027386E-4	9.688406130620887E-4	6.903852349611083E-5
85-89	0.12271827679845353	5.177889262208312E-4	4.2573756155935016E-4	5.523081879688866E-4	0.0012173792976480874	6.903852349611083E-5
90-94	0.12283564228839691	5.385004832696644E-4	4.487504027247204E-4	5.845261656004051E-4	0.0012426934229299949	6.903852349611083E-5
95-99	0.12293920007364108	5.707184609011829E-4	4.717632438900906E-4	6.259492796980716E-4	0.0012426934229299949	7.364109172918488E-5
100-104	0.12315552078059558	6.075390067657754E-4	5.039812215216091E-4	6.512634049799788E-4	0.0012611036958622914	8.054494407879597E-5
105-109	0.12344778386339576	6.397569843972937E-4	5.292953468035164E-4	6.788788143784232E-4	0.0012795139687945874	8.054494407879597E-5
110-114	0.12368251484328255	6.512634049799788E-4	5.84526165600405E-4	7.087955078934044E-4	0.0012887191052607355	8.054494407879597E-5
115-119	0.12392184839140241	6.558659732130528E-4	5.960325861830903E-4	7.133980761264786E-4	0.00131633451465918	8.054494407879597E-5
120-124	0.12441892576057441	6.627698255626639E-4	6.075390067657753E-4	7.133980761264786E-4	0.0013692640493395314	8.054494407879597E-5
125-129	0.12551433700004605	6.834813826114972E-4	6.420582685138308E-4	7.318083490587749E-4	0.0014406038569521793	9.435264877801813E-5
130-134	0.12660974823951765	7.203019284760896E-4	6.673723937957379E-4	7.640263266902932E-4	0.0015280526533805864	1.0355778524416626E-4
135-139	0.12773277488838772	7.456160537579968E-4	7.064942237768674E-4	7.893404519722005E-4	0.0016016937451097714	1.0585906936070327E-4
140-144	0.12863717954618678	7.548211902241451E-4	7.663276108068303E-4	8.169558613706449E-4	0.0016431168592074379	1.2196805817646246E-4
145-149	0.13004096285727434	7.663276108068303E-4	8.192571454871819E-4	8.422699866525522E-4	0.001728264371519308	1.3117319464261057E-4
150-154	0.13132738067841856	7.939430202052745E-4	8.560776913517743E-4	8.629815437013853E-4	0.0018065080314815668	1.3807704699222165E-4
155-159	0.1325056381460855	8.169558613706449E-4	8.744879642840705E-4	8.813918166336816E-4	0.0018341234408800109	1.495834675749068E-4
160-164	0.1336539789202375	8.491738390021632E-4	9.044046577990518E-4	9.06705941915589E-4	0.0019031619643761217	1.495834675749068E-4
165-167	0.13472484312913272	8.514751231187002E-4	9.166781730872493E-4	9.243491201423728E-4	0.0019445850784737884	1.495834675749068E-4
>>END_MODULE
