##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293767
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8776770
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.369922989892636	39.0	39.0	39.0	37.0	39.0
2	40.155909748119186	41.0	40.0	41.0	38.0	41.0
3	40.200868428818346	41.0	40.0	41.0	38.0	41.0
4	40.21123477087812	41.0	40.0	41.0	38.0	41.0
5	40.20581079372024	41.0	40.0	41.0	38.0	41.0
6	40.19546553002984	41.0	40.0	41.0	38.0	41.0
7	40.21597512524539	41.0	40.0	41.0	38.0	41.0
8	40.20281698164587	41.0	40.0	41.0	38.0	41.0
9	40.18579887589626	41.0	40.0	41.0	38.0	41.0
10-14	40.1246218369628	41.0	40.0	41.0	38.0	41.0
15-19	40.013039033721974	41.0	40.0	41.0	38.0	41.0
20-24	39.837763049504545	41.0	40.0	41.0	37.6	41.0
25-29	39.59724695987248	40.6	39.2	41.0	36.6	41.0
30-34	39.361797107591975	40.0	39.0	41.0	35.6	41.0
35-39	39.192527205338635	40.0	38.2	41.0	35.0	41.0
40-44	38.69679433322282	39.8	36.6	41.0	35.0	41.0
45-49	37.996954073081554	38.8	35.2	41.0	35.0	41.0
50-54	37.25106696427046	37.0	35.0	40.0	34.2	41.0
55-59	36.545908916378124	35.8	35.0	39.0	34.0	41.0
60-64	35.80116771887608	35.0	34.8	37.0	33.6	39.6
65-69	35.45749939898163	35.0	35.0	36.0	33.6	38.8
70-74	35.257761135360724	35.0	35.0	35.8	34.0	37.2
75-79	35.10697962918021	35.0	35.0	35.0	33.8	37.0
80-84	35.048989913145725	35.0	35.0	35.0	33.0	36.2
85-89	34.86778593947432	35.0	34.6	35.0	32.6	36.6
90-94	39.64658207318348	40.6	39.8	40.6	37.8	40.6
95-99	39.91006568576628	41.0	40.0	41.0	38.0	41.0
100-104	39.791780759354424	41.0	40.0	41.0	37.0	41.0
105-109	39.6368633752184	41.0	39.6	41.0	36.4	41.0
110-114	39.44996185729912	40.8	39.0	41.0	35.6	41.0
115-119	39.16805294571487	40.0	38.2	41.0	35.0	41.0
120-124	38.89092115793074	40.0	38.0	41.0	35.0	41.0
125-129	38.34068626407331	39.6	36.6	40.6	34.6	41.0
130-134	38.02537872013745	39.0	35.4	41.0	34.0	41.0
135-139	37.64536050584071	38.2	35.0	41.0	34.0	41.0
140-144	37.034537631056516	36.6	35.0	39.6	34.0	41.0
145-149	36.32881076815246	35.4	35.0	38.4	34.0	40.8
150-154	35.696681395958066	35.0	35.0	36.6	34.0	39.2
155-159	35.20631926274542	35.0	35.0	36.0	33.6	37.4
160-164	34.87695958804176	35.0	35.0	35.0	33.0	36.4
165-169	34.67525258535898	35.0	35.0	35.0	33.0	36.0
170-174	34.56143314408003	35.0	35.0	35.0	33.0	35.8
175-178	34.256089932731044	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	44.0
30	565.0
31	4036.0
32	19784.0
33	72911.0
34	204257.0
35	499510.0
36	1309861.0
37	2878597.0
38	3043310.0
39	735475.0
40	8417.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.45405770004227	27.350528725259977	27.54400536871765	22.651408205980104
2	22.784133570778316	27.817910233491368	27.89825869881517	21.49969749691515
3	21.421570805660853	26.879170811129832	28.79402103507327	22.905237348136044
4	21.815360320482363	26.242467331375895	29.295629257688194	22.646543090453548
5	22.641974211469595	26.732921108790592	27.969526374736947	22.655578305002866
6	22.934952152101513	26.707592884398245	27.48292367237606	22.874531291124185
7	22.825652261595096	27.116763912008636	27.26005124892187	22.797532577474403
8	22.9457306047669	27.11664997487686	27.192862522317434	22.7447568980388
9	23.203798208224665	27.059886495829332	26.817086468028673	22.919228827917333
10-14	23.045999838209273	27.22386253712926	26.908256682127934	22.82188094253353
15-19	23.01053348783208	27.219266313233685	26.9865292129109	22.78367098602333
20-24	22.94312828067729	27.0934067999959	27.127414755086438	22.836050164240376
25-29	22.886551658525857	27.22942722664488	27.008204612858716	22.87581650197054
30-34	22.902468675834047	27.272119469918888	27.007607582288244	22.81780427195882
35-39	22.888376931376804	27.25811431768179	27.03414126153471	22.819367489406694
40-44	22.878345906295824	27.30739668465734	27.045058717500858	22.769198691545977
45-49	23.02051893806036	27.21522610254114	26.921307041200805	22.842947918197698
50-54	22.979617786497766	27.257845426050814	26.979392190976863	22.783144596474557
55-59	22.93218120105688	27.251180103842305	26.992351400344315	22.8242872947565
60-64	22.908701036941835	27.273322646030373	27.013463950861194	22.804512366166595
65-69	22.900226393080825	27.242944727958008	27.022054810596607	22.834774068364556
70-74	22.85144534948506	27.33304393301864	27.019126626310136	22.796384091186166
75-79	22.872890596426704	27.3059519618265	26.975044350028544	22.84611309171825
80-84	22.869878098662717	27.332986964452754	26.96680213791634	22.830332798968186
85-89	22.87544734566361	27.340759755582067	26.934924807189887	22.848868091564437
90-94	22.147981155635392	26.96891830331434	28.274881598246587	22.60821894280369
95-99	22.771012930493704	27.06946478050242	27.226345549019733	22.933176739984145
100-104	22.868473515887505	27.188760110817096	27.031617820298564	22.911148552996828
105-109	22.719129729867632	27.207930348856184	27.235679580444994	22.837260340831193
110-114	22.74797574221331	27.052385909343812	27.22849820885499	22.971140139587888
115-119	22.73576728506504	27.11859593209166	27.160548269727137	22.98508851311616
120-124	22.652257134360184	27.1730458759292	27.229978599193466	22.944718390517142
125-129	22.63663672642555	27.17717481375981	27.224628394404494	22.961560065410147
130-134	22.73673268817436	27.17240841359263	27.161806280925855	22.929052617307153
135-139	22.776589555191055	27.177345763284517	27.137504752242457	22.908559929281967
140-144	22.756997240849415	27.217589015988263	27.16599718820021	22.85941655496211
145-149	22.726216741622675	27.217689897107206	27.196997495751145	22.859095865518974
150-154	22.616644240996628	27.367384943967117	27.269525604165295	22.746445210870956
155-159	22.616252847241167	27.34880381346206	27.2599766752187	22.774966664078075
160-164	22.560216570403405	27.461114547300713	27.304197385355057	22.67447149694083
165-169	22.466562058705296	27.537246102838964	27.34593885692016	22.650252981535584
170-174	22.464009968504097	27.63369743830492	27.332769224456094	22.569523368734888
175-178	22.43354599459794	27.686790594875315	27.328247142015428	22.55141626851132
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.5
9	4.0
10	6.5
11	10.0
12	28.5
13	52.5
14	102.0
15	239.0
16	427.5
17	761.5
18	1496.5
19	2709.5
20	4628.5
21	7563.5
22	11810.5
23	17412.0
24	24737.5
25	33767.0
26	44242.0
27	56574.5
28	70521.0
29	85789.0
30	101618.0
31	119062.5
32	137666.5
33	156598.0
34	177018.5
35	198352.5
36	220245.5
37	242236.0
38	263373.0
39	283928.0
40	303803.5
41	321418.5
42	336072.0
43	346210.5
44	352080.0
45	354826.5
46	352812.0
47	347158.5
48	338443.5
49	325785.0
50	310670.0
51	293430.0
52	275365.5
53	256889.0
54	237950.5
55	219099.0
56	200120.5
57	181663.5
58	164208.5
59	147807.0
60	132219.5
61	117510.5
62	103975.5
63	90979.5
64	78473.5
65	67179.5
66	56868.5
67	47086.5
68	38149.0
69	30517.5
70	23824.5
71	18114.0
72	13434.0
73	9690.5
74	6780.5
75	4713.0
76	3278.5
77	2164.0
78	1435.5
79	950.0
80	575.0
81	350.5
82	225.0
83	112.0
84	42.0
85	29.0
86	19.5
87	8.5
88	3.5
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1107.0
92-93	2243.0
94-95	3449.0
96-97	4321.0
98-99	5552.0
100-101	6175.0
102-103	7438.0
104-105	8502.0
106-107	9287.0
108-109	10328.0
110-111	11263.0
112-113	11890.0
114-115	12546.0
116-117	13137.0
118-119	13787.0
120-121	14504.0
122-123	15128.0
124-125	15808.0
126-127	16357.0
128-129	16918.0
130-131	17864.0
132-133	20086.0
134-135	92321.0
136-137	82297.0
138-139	93069.0
140-141	79436.0
142-143	82660.0
144-145	80467.0
146-147	83823.0
148-149	86147.0
150-151	83920.0
152-153	81254.0
154-155	79246.0
156-157	80377.0
158-159	82823.0
160-161	87910.0
162-163	87764.0
164-165	91352.0
166-167	88635.0
168-169	93384.0
170-171	93575.0
172-173	103418.0
174-175	104546.0
176-177	275822.0
178-179	6424834.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.02872345866
#Duplication Level	Percentage of total
1	90.56015356107154
2	8.157248150459798
3	0.9572615249318498
4	0.20738866664925917
5	0.04907559290027628
6	0.02673390314906389
7	0.013442879894542549
8	0.008319440813618033
9	0.0052290714621062865
>10	0.015147208667958559
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	1.1393713176943226E-4	0.0	0.0	2.278742635388645E-5	0.0	0.0
3	1.253308449463755E-4	2.278742635388645E-5	0.0	3.418113953082968E-5	0.0	0.0
4	1.253308449463755E-4	2.278742635388645E-5	0.0	3.418113953082968E-5	0.0	0.0
5	1.367245581233187E-4	6.836227906165935E-5	1.1393713176943226E-5	4.55748527077729E-5	0.0	0.0
6	1.4811827130026194E-4	9.11497054155458E-5	3.418113953082968E-5	4.55748527077729E-5	0.0	0.0
7	1.4811827130026194E-4	9.11497054155458E-5	3.418113953082968E-5	4.55748527077729E-5	0.0	0.0
8	1.709056976541484E-4	1.1393713176943226E-4	4.55748527077729E-5	5.696856588471613E-5	0.0	0.0
9	1.709056976541484E-4	1.253308449463755E-4	4.55748527077729E-5	6.836227906165935E-5	0.0	0.0
10-14	1.959718666434235E-4	1.4583952866487328E-4	6.152605115549341E-5	1.0482216122787767E-4	1.5951198447720513E-5	0.0
15-19	2.5066168989275095E-4	1.7318444028953703E-4	7.291976433243664E-5	1.41282043394096E-4	2.5066168989275095E-5	0.0
20-24	2.962365426005239E-4	1.959718666434235E-4	1.0937964649865498E-4	1.5951198447720517E-4	4.7853595343161546E-5	0.0
25-29	3.304176821313536E-4	2.2331677826808722E-4	1.41282043394096E-4	1.9141438137264618E-4	7.519850696782529E-5	0.0
30-34	4.830934387023928E-4	2.278742635388645E-4	1.8913563873725758E-4	2.0052935191420081E-4	1.1393713176943226E-4	0.0
35-39	0.001066451553361886	2.552191751635282E-4	2.1648055036192128E-4	2.3698923408041911E-4	1.6862695501875975E-4	6.8362279061659355E-6
40-44	0.0016543671532921564	2.894003146943579E-4	2.3015300617425317E-4	2.620554030696942E-4	3.0990899841285577E-4	2.278742635388645E-5
45-49	0.002249118981128593	2.9851528523591254E-4	2.780066015174147E-4	2.7572785888202607E-4	3.987799611930129E-4	2.7344911624663742E-5
50-54	0.0028438708089650287	3.3269642476674223E-4	3.1902396895441034E-4	3.2586019686057626E-4	4.62584754983895E-4	3.873862480160697E-5
55-59	0.0032768319096888716	3.577625937560173E-4	3.645988216621832E-4	3.668775642975719E-4	5.377832619517202E-4	5.4689823249327484E-5
60-64	0.003577625937560173	3.965012185576242E-4	4.1700990227612207E-4	3.873862480160697E-4	6.585566216273184E-4	6.380479379088205E-5
65-69	0.003953618472399299	4.3979732863000853E-4	4.4207607126539713E-4	4.147311596407334E-4	7.633787828551961E-4	7.519850696782529E-5
70-74	0.008410839067219488	4.671422402546723E-4	4.808146960670041E-4	4.3979732863000853E-4	8.294623192814668E-4	9.11497054155458E-5
75-79	0.08639169079285433	5.26389548774777E-4	4.990446371501134E-4	4.7625721079622683E-4	8.887096278015717E-4	1.0937964649865497E-4
80-84	0.11872704878901921	5.674069162117726E-4	5.149958355978337E-4	5.081596076916678E-4	0.0010505003549141655	1.253308449463755E-4
85-89	0.11887744580295485	5.970305704718251E-4	5.446194898578862E-4	5.377832619517202E-4	0.0012829321037238074	1.253308449463755E-4
90-94	0.11904151527270282	6.243754820964889E-4	5.765218867533272E-4	5.765218867533272E-4	0.0013011620448069164	1.253308449463755E-4
95-99	0.11921014222772157	6.35769195273432E-4	6.107030262841569E-4	6.289329673672661E-4	0.001310277015348471	1.253308449463755E-4
100-104	0.11936509672692801	6.494416510857639E-4	6.699503348042618E-4	6.676715921688731E-4	0.0013171132432546369	1.253308449463755E-4
105-109	0.11954283865248834	6.767865627104277E-4	7.041314743350914E-4	6.950165037935367E-4	0.0013216707285254143	1.253308449463755E-4
110-114	0.11974336800440252	7.041314743350914E-4	7.383126138659211E-4	7.155251875120346E-4	0.001337621926973135	1.253308449463755E-4
115-119	0.11994161861368136	7.497063270428641E-4	7.611000402198074E-4	7.383126138659211E-4	0.0013672455812331872	1.253308449463755E-4
120-124	0.1206115689484856	7.816087239383054E-4	7.907236944798598E-4	7.930024371152484E-4	0.0014173779192117372	1.3216707285254142E-4
125-129	0.1218945010522094	8.066748929275805E-4	8.499710029999648E-4	8.249048340106896E-4	0.0014971339114503398	1.4583952866487328E-4
130-134	0.12312046459004851	8.340198045522441E-4	8.795946572600171E-4	8.408560324584102E-4	0.0016110710432197723	1.4811827130026194E-4
135-139	0.12433047692943985	8.773159146246285E-4	9.046608262492922E-4	8.613647161769079E-4	0.0017181719470830386	1.4811827130026194E-4
140-144	0.1254857994455819	9.11497054155458E-4	9.547931642278424E-4	8.932671130723489E-4	0.0017888129687800864	1.4811827130026194E-4
145-149	0.1266980905276087	9.228907673324013E-4	0.001014040472747947	9.001033409785149E-4	0.0018936351300079643	1.5495449920642788E-4
150-154	0.12802659748404024	9.320057378739558E-4	0.0010687302959972746	9.342844805093444E-4	0.002023523460225117	1.5951198447720517E-4
155-159	0.1293232020435764	9.616293921340082E-4	0.0010710090386326632	9.661868774047856E-4	0.0021169519082760513	1.5951198447720517E-4
160-164	0.13054688683878007	9.798593332171176E-4	0.0011097476634342703	9.730231053109515E-4	0.002196707900514654	1.5951198447720517E-4
165-167	0.13162017462004816	0.001002646759571004	0.0011165838913404361	0.0010064446639633186	0.0022255719738962436	1.67107793261834E-4
>>END_MODULE
