##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293768
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8738194
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.38952785896033	39.0	39.0	39.0	37.0	39.0
2	40.17546383154231	41.0	40.0	41.0	38.0	41.0
3	40.21576220440974	41.0	40.0	41.0	38.0	41.0
4	40.22505119478922	41.0	40.0	41.0	38.0	41.0
5	40.21841950407602	41.0	40.0	41.0	38.0	41.0
6	40.21098295597466	41.0	40.0	41.0	38.0	41.0
7	40.23275724938128	41.0	40.0	41.0	38.0	41.0
8	40.218880926653725	41.0	40.0	41.0	38.0	41.0
9	40.20569879771495	41.0	40.0	41.0	38.0	41.0
10-14	40.14679143081511	41.0	40.0	41.0	38.0	41.0
15-19	40.035358931147556	41.0	40.0	41.0	38.0	41.0
20-24	39.86917552986349	41.0	40.0	41.0	37.8	41.0
25-29	39.62260748616934	40.8	39.2	41.0	36.8	41.0
30-34	39.37880113442206	40.0	39.0	41.0	35.6	41.0
35-39	39.19960348786031	40.0	38.2	41.0	35.0	41.0
40-44	38.70207722556858	39.8	36.6	41.0	35.0	41.0
45-49	38.001697032590485	38.8	35.2	41.0	35.0	41.0
50-54	37.25368779864581	37.0	35.0	40.0	34.4	41.0
55-59	36.5455086028074	35.8	35.0	38.8	34.0	41.0
60-64	35.80510849267023	35.0	34.8	36.8	33.6	39.6
65-69	35.47316665205648	35.0	35.0	36.0	33.8	39.0
70-74	35.28070816463905	35.0	35.0	35.8	34.0	37.2
75-79	35.12891846988062	35.0	35.0	35.0	34.0	37.0
80-84	35.07261411225248	35.0	35.0	35.0	33.2	36.2
85-89	34.895414246925625	35.0	34.6	35.0	32.6	36.6
90-94	39.63812192044299	40.6	39.8	40.6	37.8	40.6
95-99	39.92085411910723	41.0	40.0	41.0	38.0	41.0
100-104	39.80809431027781	41.0	40.0	41.0	37.0	41.0
105-109	39.65877506420674	41.0	39.6	41.0	36.4	41.0
110-114	39.476206150751736	41.0	39.0	41.0	35.6	41.0
115-119	39.197322214243016	40.0	38.4	41.0	35.0	41.0
120-124	38.91814889639228	40.0	38.0	41.0	35.0	41.0
125-129	38.36667688916169	39.6	36.6	40.6	34.6	41.0
130-134	38.05614723383823	39.0	35.4	41.0	34.0	41.0
135-139	37.66025119922496	38.2	35.0	41.0	34.4	41.0
140-144	37.0370397486342	36.6	35.0	39.6	34.0	41.0
145-149	36.33711056245037	35.4	35.0	38.2	34.0	40.8
150-154	35.71484384862534	35.0	35.0	36.6	34.0	39.0
155-159	35.23806097344688	35.0	35.0	36.0	34.0	37.4
160-164	34.91465841563222	35.0	35.0	35.0	33.4	36.2
165-169	34.72409077897747	35.0	35.0	35.0	33.0	36.0
170-174	34.61439202250721	35.0	35.0	35.0	33.0	36.0
175-178	34.32394933416197	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	43.0
30	483.0
31	3833.0
32	19247.0
33	70384.0
34	198406.0
35	480335.0
36	1275154.0
37	2856397.0
38	3062155.0
39	761146.0
40	10611.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.546054711076454	27.28101481839382	27.45949563491037	22.71343483561935
2	22.817781340171663	27.798295620353585	27.828828245287298	21.55509479418745
3	21.461013568707678	26.812245184760148	28.73709372897878	22.9896475175534
4	21.840931890502773	26.19345599330937	29.226588468967385	22.739023647220467
5	22.701819163090224	26.672033145521834	27.933231969901335	22.692915721486614
6	22.983547858974063	26.668931818176617	27.39591270232728	22.951607620522044
7	22.878079841212042	27.069861346635243	27.203321418590615	22.848737393562104
8	23.015636869586555	27.05591109558794	27.139727041995176	22.788724992830325
9	23.277040999547506	26.987258465536474	26.746670994029202	22.989029540886825
10-14	23.108143398967794	27.16705076586764	26.848474639038685	22.876331196125882
15-19	23.06568611317167	27.153118825240092	26.936682797383533	22.84451226420471
20-24	22.99712503521895	27.04909046423094	27.058096901945643	22.89568759860447
25-29	22.950067256460546	27.14897609277157	26.9616078562687	22.939348794499185
30-34	22.985133999084937	27.19198726876515	26.942214832950604	22.88066389919931
35-39	22.965308392100244	27.186713867877042	26.966201482823564	22.881776257199142
40-44	22.937311760301956	27.24924852892943	26.989226835659636	22.824212875108977
45-49	23.07984464524363	27.164496462312464	26.84719519845863	22.90846369398528
50-54	23.0350550697318	27.19696770293724	26.927088137434346	22.840889089896606
55-59	22.994492912379837	27.174214717594964	26.947723980492995	22.883568389532208
60-64	22.97599938843198	27.203898196812755	26.952020062726923	22.86808235202835
65-69	22.958226837261797	27.169797328830192	26.983463631043207	22.888512202864803
70-74	22.908585000516123	27.26877888039565	26.959014643071555	22.863621476016668
75-79	22.938849835560987	27.238353829177974	26.918088566127054	22.90470776913399
80-84	22.958110108335887	27.243432681856227	26.906241724548575	22.892215485259314
85-89	22.94586730393031	27.26806019642045	26.885063435304822	22.901009064344414
90-94	22.225488083323043	26.886008460442095	28.20697220750677	22.68153124872809
95-99	22.864997462435845	26.98005392999806	27.167713146452144	22.98723546111395
100-104	22.94293017042942	27.07771747307549	26.985075647773765	22.994276708721326
105-109	22.812511956577783	27.12735515679449	27.16364908622469	22.896483800403036
110-114	22.832321908818752	26.965851316361267	27.184160316861238	23.01766645795874
115-119	22.808897336887796	27.02612186341396	27.115619782589658	23.049361017108588
120-124	22.744034841914626	27.08948345557689	27.165447581542253	23.001034120966235
125-129	22.72647544206086	27.100007221365914	27.152522845781103	23.020994490792127
130-134	22.802097977704715	27.069332142291408	27.124065428318627	23.004504451685246
135-139	22.860577561542414	27.090532593509124	27.05526978786086	22.993620057087604
140-144	22.84481489615896	27.14043275391657	27.110815197241344	22.903937152683117
145-149	22.810192703273614	27.134945638342145	27.126462992235673	22.928398666148563
150-154	22.697857026992306	27.254468327027265	27.221715622677667	22.825959023302765
155-159	22.692167903585673	27.25392290275941	27.200207655737817	22.853701537917097
160-164	22.640005888575413	27.377287844324798	27.217407126731906	22.76529914036788
165-169	22.58042169776807	27.425300056256418	27.26068159579832	22.733596650177187
170-174	22.580948564825615	27.515344554102406	27.236204957098842	22.667501923973138
175-178	22.561826990564583	27.552670024460124	27.224612450285978	22.66089053468931
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	5.5
11	16.0
12	27.0
13	46.0
14	96.5
15	210.5
16	423.5
17	813.5
18	1476.5
19	2662.0
20	4537.0
21	7266.5
22	11241.0
23	16856.5
24	24126.5
25	32830.5
26	43135.5
27	55361.0
28	68435.5
29	82974.0
30	99206.0
31	116384.5
32	134514.0
33	153794.0
34	173611.0
35	194538.0
36	216482.5
37	238507.0
38	260387.5
39	281014.5
40	300359.5
41	317429.5
42	331991.0
43	342860.5
44	349346.5
45	352379.5
46	350699.0
47	345236.0
48	336748.5
49	324965.5
50	310906.0
51	294445.0
52	276569.5
53	258133.0
54	238863.5
55	219483.0
56	200781.5
57	182838.5
58	165676.5
59	149541.0
60	134317.5
61	119705.0
62	105993.5
63	92957.0
64	80695.0
65	69070.5
66	58234.5
67	48323.0
68	39142.0
69	31192.5
70	24470.5
71	18677.0
72	13870.0
73	10036.5
74	7163.0
75	5102.0
76	3542.0
77	2341.5
78	1508.5
79	981.5
80	607.5
81	355.5
82	206.0
83	110.5
84	60.5
85	30.0
86	17.0
87	6.0
88	2.0
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1070.0
92-93	2163.0
94-95	4313.0
96-97	5291.0
98-99	5442.0
100-101	6296.0
102-103	7329.0
104-105	8274.0
106-107	9755.0
108-109	10590.0
110-111	11585.0
112-113	12994.0
114-115	14060.0
116-117	13867.0
118-119	13718.0
120-121	15831.0
122-123	15005.0
124-125	17080.0
126-127	16826.0
128-129	17330.0
130-131	17893.0
132-133	19739.0
134-135	90680.0
136-137	84336.0
138-139	83745.0
140-141	82290.0
142-143	77014.0
144-145	83281.0
146-147	84153.0
148-149	93474.0
150-151	86755.0
152-153	78549.0
154-155	81752.0
156-157	77113.0
158-159	79010.0
160-161	83129.0
162-163	80148.0
164-165	82587.0
166-167	85702.0
168-169	105088.0
170-171	90833.0
172-173	97523.0
174-175	97059.0
176-177	265247.0
178-179	6422275.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.23468845519106
#Duplication Level	Percentage of total
1	90.94440019051765
2	7.822977403289482
3	0.9462182823973937
4	0.18605965484809958
5	0.04908210586046654
6	0.02468136733993187
7	0.011376845957443944
8	0.002080298242045041
9	0.0020921229886231405
>10	0.009612756957607195
>50	0.0014189716012590842
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	5.722006171984737E-5	0.0	1.1444012343969475E-5	0.0	0.0	0.0
2	2.746562962552674E-4	0.0	1.1444012343969475E-5	0.0	0.0	0.0
3	2.861003085992369E-4	0.0	2.288802468793895E-5	1.1444012343969475E-5	0.0	0.0
4	2.861003085992369E-4	0.0	2.288802468793895E-5	4.57760493758779E-5	0.0	0.0
5	2.975443209432063E-4	1.1444012343969475E-5	2.288802468793895E-5	4.57760493758779E-5	0.0	0.0
6	3.089883332871758E-4	1.1444012343969475E-5	2.288802468793895E-5	4.57760493758779E-5	0.0	0.0
7	3.089883332871758E-4	3.4332037031908426E-5	2.288802468793895E-5	4.57760493758779E-5	0.0	0.0
8	3.089883332871758E-4	4.57760493758779E-5	2.288802468793895E-5	6.866407406381685E-5	0.0	0.0
9	3.089883332871758E-4	5.722006171984737E-5	2.288802468793895E-5	6.866407406381685E-5	0.0	0.0
10-14	3.227211480999392E-4	7.324167900140463E-5	3.6620839500702324E-5	8.697449381416801E-5	0.0	0.0
15-19	3.410315678502904E-4	1.0986251850210694E-4	4.806485184467179E-5	9.61297036893436E-5	0.0	0.0
20-24	3.7994120981978656E-4	1.373281481276337E-4	5.722006171984737E-5	1.2130653084607643E-4	2.975443209432063E-5	0.0
25-29	4.2342845672687057E-4	1.9225940737868717E-4	7.324167900140463E-5	1.6708258022195434E-4	6.179766665743517E-5	0.0
30-34	5.699118147296799E-4	2.3345785181697732E-4	1.0299611109572527E-4	1.8081539503471772E-4	1.0986251850210694E-4	0.0
35-39	0.0011833108763664437	2.494794690985345E-4	1.6479377775316042E-4	1.922594073786872E-4	1.6021617281557267E-4	2.5176827156732845E-5
40-44	0.001718890654064215	2.6092348144250406E-4	2.0141461725386276E-4	1.9912581478506884E-4	2.265914444105956E-4	4.1198444438290106E-5
45-49	0.0023437337280449483	2.975443209432063E-4	2.288802468793895E-4	2.288802468793895E-4	3.1356593822476364E-4	5.264245678225959E-5
50-54	0.0028495590736483992	3.112771357559697E-4	2.494794690985345E-4	2.792339011928552E-4	4.051180369765194E-4	6.866407406381685E-5
55-59	0.0031699914192795443	3.433203703190842E-4	2.5176827156732845E-4	3.204323456311453E-4	4.8522612338430576E-4	7.781928393899243E-5
60-64	0.0034995789747858655	3.822300122885804E-4	2.975443209432063E-4	3.639195925382293E-4	5.653342097920921E-4	9.15520987517558E-5
65-69	0.003781101678447514	4.028292345077255E-4	3.2043234563114534E-4	3.936740246325499E-4	6.477310986686722E-4	9.15520987517558E-5
70-74	0.008189335233344554	4.3945007400842783E-4	3.433203703190842E-4	4.188508517892828E-4	7.392831974204281E-4	9.38409012205497E-5
75-79	0.08693329536972973	4.508940863523973E-4	3.8680761722616826E-4	4.5776049375877896E-4	8.262576912345962E-4	1.1672892590848863E-4
80-84	0.12020103925364897	4.8751492585309957E-4	3.959628271013438E-4	4.966701357282751E-4	9.81896259112581E-4	1.373281481276337E-4
85-89	0.1204184754881844	5.126917530098325E-4	4.2571725919566454E-4	5.195581604162142E-4	0.0012748629751181996	1.373281481276337E-4
90-94	0.12050773878446737	5.287133702913898E-4	4.669157036339545E-4	5.355797776977714E-4	0.0012931733948685507	1.373281481276337E-4
95-99	0.12061531250050068	5.538901974481225E-4	5.081141480722447E-4	6.08821456699176E-4	0.0013137726170876958	1.373281481276337E-4
100-104	0.12075492945109709	5.676230122608861E-4	5.195581604162142E-4	6.5230870360626E-4	0.001327505431900459	1.373281481276337E-4
105-109	0.12094947766094458	5.813558270736493E-4	5.424461851041531E-4	6.683303208878174E-4	0.001329794234369253	1.373281481276337E-4
110-114	0.12110969383376016	5.927998394176187E-4	5.6991181472968E-4	7.026623579197257E-4	0.0013412382467132224	1.373281481276337E-4
115-119	0.1213088196485452	6.179766665743516E-4	5.950886418864126E-4	7.186839752012831E-4	0.0013641262714011615	1.373281481276337E-4
120-124	0.12184211062377419	6.454422961998783E-4	6.339982838559088E-4	7.553048147019853E-4	0.0014099023207770392	1.373281481276337E-4
125-129	0.1230414431174222	6.706191233566113E-4	6.5230870360626E-4	7.713264319835426E-4	0.0014671223824968868	1.4877216047160316E-4
130-134	0.12415380111725605	6.866407406381685E-4	6.545975060750539E-4	7.781928393899242E-4	0.0015220536417479402	1.4877216047160316E-4
135-139	0.12552708259853235	6.889295431069624E-4	6.706191233566112E-4	7.873480492650998E-4	0.0015746960985301996	1.5106096294039705E-4
140-144	0.1267287038946492	6.98084752982138E-4	6.935071480445503E-4	7.965032591402754E-4	0.0016273385553124593	1.6479377775316042E-4
145-149	0.12782732907967023	7.278391850764586E-4	7.324167900140463E-4	8.193912838282144E-4	0.0016891362219698942	1.7166018515954212E-4
150-154	0.12887560061037784	7.736152344523365E-4	7.667488270459548E-4	8.26257691234596E-4	0.0017303346664081843	1.7166018515954212E-4
155-159	0.13026719251140456	7.919256542026876E-4	7.759040369211304E-4	8.377017035785656E-4	0.001764666703440093	1.7166018515954212E-4
160-164	0.13146881380752135	8.10236073953039E-4	7.896368517338936E-4	8.628785307352985E-4	0.0018264643700975282	1.8081539503471772E-4
165-167	0.1328878713381735	8.163395472031559E-4	8.087102056405095E-4	8.773742797043264E-4	0.001880632695192317	1.9073353906615793E-4
>>END_MODULE
