##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293770
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9028424
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.01798087905486	41.0	40.0	41.0	38.0	41.0
2	40.16455695922123	41.0	40.0	41.0	38.0	41.0
3	40.21388937869998	41.0	40.0	41.0	38.0	41.0
4	40.2418498510925	41.0	40.0	41.0	38.0	41.0
5	40.253703193381256	41.0	40.0	41.0	38.0	41.0
6	40.27939239450872	41.0	40.0	41.0	39.0	41.0
7	40.23961933998669	41.0	40.0	41.0	39.0	41.0
8	40.25095243643852	41.0	40.0	41.0	39.0	41.0
9	40.21911454313621	41.0	40.0	41.0	39.0	41.0
10-14	40.182264324316186	41.0	40.0	41.0	38.2	41.0
15-19	40.084635147839755	41.0	40.0	41.0	38.0	41.0
20-24	39.93908919208934	41.0	40.0	41.0	38.0	41.0
25-29	39.711360011448285	41.0	39.6	41.0	36.8	41.0
30-34	39.36923799768376	40.2	38.8	41.0	35.4	41.0
35-39	38.89833740639562	40.0	37.6	41.0	35.0	41.0
40-44	38.41483262195041	39.6	36.4	41.0	35.0	41.0
45-49	37.89148442740394	38.8	35.0	41.0	35.0	41.0
50-54	37.30482219266618	37.0	35.0	40.0	35.0	41.0
55-59	36.62159490958776	35.8	35.0	39.0	34.4	41.0
60-64	35.796522161564404	35.0	34.8	36.8	33.4	39.6
65-69	35.57373390970561	35.0	35.0	36.0	34.0	38.8
70-74	35.31779123355305	35.0	35.0	35.8	34.0	37.0
75-79	35.157619602269456	35.0	35.0	35.0	34.0	36.6
80-84	35.09441264610523	35.0	35.0	35.0	34.0	36.0
85-89	35.9765042492466	36.2	35.8	36.2	34.6	37.0
90-94	40.073206619696684	41.0	40.0	41.0	38.0	41.0
95-99	40.02430386265557	41.0	40.0	41.0	38.0	41.0
100-104	39.9092917722006	41.0	40.0	41.0	37.8	41.0
105-109	39.76685589338275	41.0	40.0	41.0	37.0	41.0
110-114	39.56766936988995	41.0	39.2	41.0	35.8	41.0
115-119	39.276193254109614	40.2	38.6	41.0	35.0	41.0
120-124	38.89035972757997	40.0	37.6	41.0	35.0	41.0
125-129	37.906791482152244	39.0	36.0	40.2	34.2	40.6
130-134	38.11832612072526	39.0	35.2	41.0	35.0	41.0
135-139	37.649896176576526	38.0	35.0	40.8	35.0	41.0
140-144	36.965845599000176	36.4	35.0	39.4	34.6	41.0
145-149	36.282115645544955	35.2	35.0	37.8	34.0	40.4
150-154	35.6876718074336	35.0	35.0	36.4	34.0	39.0
155-159	35.24508880275693	35.0	35.0	35.8	34.0	37.0
160-164	34.96642100486454	35.0	35.0	35.0	34.0	36.2
165-169	34.80958664647229	35.0	35.0	35.0	34.0	36.0
170-174	34.722076961072915	35.0	35.0	35.0	34.0	36.0
175-176	34.46827519311019	35.0	34.5	35.0	33.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	6.0
29	39.0
30	474.0
31	3457.0
32	16932.0
33	59750.0
34	165952.0
35	430630.0
36	1284577.0
37	2855100.0
38	3258530.0
39	935597.0
40	17380.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.98783264941921	27.87071143313606	28.30863947018882	20.83281644725591
2	20.204323589587727	26.03833182845644	30.443718637937252	23.31362594401858
3	21.031987421060418	25.401853080892078	31.18357090894269	22.38258858910481
4	22.287865523373735	26.034776390652453	28.82911790584935	22.84824018012446
5	22.661972898038464	26.318070573557467	28.095678714247356	22.924277814156714
6	22.476503097329058	27.11228449173411	27.86981426658739	22.541398144349444
7	22.592348343409657	26.930392281089148	27.680401363515937	22.796858011985258
8	23.019765132873687	26.870005219072564	27.228805381758765	22.88142426629498
9	22.505677624356142	27.41012163363174	27.550134995875247	22.534065746136868
10-14	22.806565132519253	27.063055523311707	27.366568074339444	22.763811269829596
15-19	22.581992161644155	27.195065273850673	27.590841989698312	22.632100574806856
20-24	22.60369030076567	27.01488764816539	27.63611456440238	22.745307486666555
25-29	22.677120613741668	27.06597076078837	27.409997580973155	22.846911044496803
30-34	22.579271864059553	27.206642045167573	27.464972845759128	22.749113245013746
35-39	22.58322382732579	27.202368874124655	27.4569027772732	22.75750452127636
40-44	22.65199773515289	27.148902178276074	27.377517936685297	22.82158214988574
45-49	22.70929234160912	27.157067501481986	27.328100674048983	22.80553948285991
50-54	22.75628614695101	27.181767271895957	27.30738166483984	22.754564916313193
55-59	22.760455202369762	27.130296494714912	27.282604361514256	22.826643941401066
60-64	22.654837654943986	27.234363383908423	27.351652957371076	22.759146003776518
65-69	22.719367189666766	27.199732755129798	27.285543966477427	22.795356088726006
70-74	22.693548730099515	27.23113358433321	27.264906920631994	22.810410764935277
75-79	22.627322332225425	27.259060939096347	27.320163519125817	22.793453209552407
80-84	22.70182702983378	27.29096683983827	27.247450939388756	22.75975519093919
85-89	22.70152132863942	27.37665842897941	27.448580173018016	22.473240069363158
90-94	21.67931100785791	26.37096009327748	29.20436834200892	22.745360556855694
95-99	22.62842113102011	27.184578053366927	27.505351677194362	22.681649138418603
100-104	22.56300113803839	27.25292635192917	27.489863232294944	22.6942092777375
105-109	22.500829279872903	27.233366482294052	27.643860853162955	22.621943384670086
110-114	22.53943775056026	27.083089349996758	27.55007596240413	22.827396937038856
115-119	22.507657170947066	27.313524420690605	27.448131117988932	22.730687290373393
120-124	22.521359476219533	27.269148973577018	27.443288999544368	22.766202550659084
125-129	22.520030514993945	27.275323391330197	27.423831251401154	22.780814842274705
130-134	22.613122945139054	27.27185002562178	27.358875519830118	22.75615150940905
135-139	22.711249226835534	27.303903647036915	27.29007751189351	22.694769614234044
140-144	22.65538467976916	27.316064980911612	27.321911249634873	22.706639089684355
145-149	22.55924729130328	27.44954175174118	27.36813697902067	22.623073977934872
150-154	22.56571153213932	27.490154213500016	27.35281399645449	22.591320257906176
155-159	22.531418278506436	27.513722083877106	27.34539215147253	22.609467486143924
160-164	22.434323935125494	27.668519953967834	27.40944626561777	22.487709845288897
165-169	22.462890119196675	27.696222576195666	27.377657023534535	22.46323028107312
170-174	22.41232959920058	27.750191159434557	27.397928668797363	22.439550572567505
175-176	22.290780576699365	27.868136882573346	27.456001205714962	22.385081335012323
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	6.5
10	18.0
11	44.0
12	87.0
13	164.5
14	331.0
15	567.0
16	953.5
17	1572.0
18	2620.5
19	4279.0
20	6870.5
21	10732.5
22	16096.5
23	22983.0
24	31677.0
25	42303.5
26	54245.0
27	67543.0
28	82291.0
29	98141.0
30	114946.5
31	132942.0
32	151169.5
33	169866.5
34	189791.5
35	211087.0
36	234202.5
37	257617.5
38	280442.5
39	302930.5
40	323666.0
41	341164.5
42	354782.0
43	365508.5
44	370768.5
45	369024.5
46	362256.0
47	351034.5
48	335151.5
49	314826.5
50	293226.0
51	271706.5
52	250208.5
53	229533.5
54	210787.0
55	193631.5
56	178127.0
57	165149.0
58	153367.0
59	142481.0
60	132233.5
61	122392.5
62	112696.5
63	102207.0
64	91323.0
65	79980.0
66	69107.5
67	58659.5
68	48271.5
69	38712.5
70	30380.0
71	23235.0
72	17244.0
73	12525.0
74	8764.0
75	5974.5
76	3988.0
77	2550.5
78	1608.5
79	975.5
80	578.5
81	334.0
82	172.5
83	95.0
84	42.5
85	17.5
86	9.0
87	6.0
88	2.5
89	1.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	646.0
92-93	1717.0
94-95	2867.0
96-97	3879.0
98-99	3916.0
100-101	4406.0
102-103	6636.0
104-105	5983.0
106-107	5958.0
108-109	7469.0
110-111	7202.0
112-113	7553.0
114-115	8847.0
116-117	7876.0
118-119	8273.0
120-121	9407.0
122-123	10071.0
124-125	9982.0
126-127	10361.0
128-129	10820.0
130-131	11933.0
132-133	49984.0
134-135	86043.0
136-137	97405.0
138-139	79309.0
140-141	80389.0
142-143	83360.0
144-145	78865.0
146-147	73412.0
148-149	62873.0
150-151	74269.0
152-153	64402.0
154-155	67942.0
156-157	71816.0
158-159	71140.0
160-161	65425.0
162-163	71091.0
164-165	82885.0
166-167	76839.0
168-169	78903.0
170-171	85089.0
172-173	94751.0
174-175	146242.0
176-177	7140188.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.86724914601402
#Duplication Level	Percentage of total
1	88.37983221675123
2	9.96753664798624
3	1.2478274793439879
4	0.23977643840263752
5	0.08377429165209924
6	0.031846118865590085
7	0.014461597030440775
8	0.016618702052172
9	0.006266322831086574
>10	0.010717299162993176
>50	0.0013428859214840673
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.107613023048098E-5	2.215226046096196E-5	1.107613023048098E-5	0.0	1.107613023048098E-5	0.0
2	1.107613023048098E-5	3.322839069144294E-5	3.322839069144294E-5	0.0	1.107613023048098E-5	0.0
3	1.107613023048098E-5	3.322839069144294E-5	3.322839069144294E-5	1.107613023048098E-5	1.107613023048098E-5	0.0
4	2.215226046096196E-5	3.322839069144294E-5	3.322839069144294E-5	2.215226046096196E-5	1.107613023048098E-5	0.0
5	2.215226046096196E-5	3.322839069144294E-5	4.430452092192392E-5	3.322839069144294E-5	1.107613023048098E-5	0.0
6	2.215226046096196E-5	3.322839069144294E-5	5.538065115240489E-5	5.538065115240489E-5	1.107613023048098E-5	0.0
7	2.215226046096196E-5	3.322839069144294E-5	6.645678138288587E-5	6.645678138288587E-5	1.107613023048098E-5	0.0
8	3.322839069144294E-5	3.322839069144294E-5	6.645678138288587E-5	8.860904184384784E-5	1.107613023048098E-5	0.0
9	5.538065115240489E-5	4.430452092192392E-5	6.645678138288587E-5	9.96851720743288E-5	1.107613023048098E-5	0.0
10-14	5.7595877198501086E-5	5.0950199060212505E-5	1.1740698044309837E-4	1.0854607625871359E-4	1.107613023048098E-5	0.0
15-19	8.196336370555926E-5	7.088723347507827E-5	1.6614195345721468E-4	1.3512878881186794E-4	6.202632929069349E-5	0.0
20-24	1.0854607625871359E-4	1.063308502126174E-4	2.104464743791386E-4	1.8829421391817664E-4	1.3955924090406033E-4	0.0
25-29	1.3291356276577175E-4	1.3069833671967555E-4	2.2816828274790815E-4	2.503205432088701E-4	1.8164853577988806E-4	0.0
30-34	4.0538636643560385E-4	1.7500285764159948E-4	2.4367486507058156E-4	3.079164204073712E-4	2.2816828274790815E-4	2.215226046096196E-6
35-39	0.0011408414137395405	2.0601602228694622E-4	2.724728036698321E-4	3.699427496980647E-4	3.212077766839484E-4	1.107613023048098E-5
40-44	0.0017478133503698984	2.3702918693229298E-4	2.9684029017689026E-4	4.1203204457389243E-4	4.496908873575277E-4	1.107613023048098E-5
45-49	0.002323772122354909	2.835489339003131E-4	3.2785345482223697E-4	4.231081748043734E-4	5.693130938467224E-4	1.772180836876957E-5
50-54	0.0026183971864857034	3.0127074226908265E-4	3.610818455136799E-4	4.474756613114315E-4	7.288093691656484E-4	2.215226046096196E-5
55-59	0.0028709329557406694	3.234230027300446E-4	3.765884278363533E-4	4.6962792177239346E-4	9.50331973775268E-4	4.651974696802011E-5
60-64	0.003083594656165904	3.5443616737539134E-4	4.275386268965658E-4	4.7627359991068215E-4	0.001109828249094194	5.538065115240489E-5
65-69	0.0032696736440379847	3.9874068829731516E-4	4.541213394497201E-4	5.139324426943174E-4	0.0012405265858138697	6.645678138288587E-5
70-74	0.010934355763530823	4.7405837386458585E-4	4.7184314781848966E-4	5.626674157084337E-4	0.001357933566256968	9.746994602823261E-5
75-79	0.06424598578888188	5.338694771091832E-4	5.050715385099327E-4	6.180480668608386E-4	0.0015107841634376055	1.1076130230480978E-4
80-84	0.07600440564156048	5.626674157084337E-4	5.294390250169908E-4	6.955809784742055E-4	0.0017256610899089365	1.1076130230480978E-4
85-89	0.07611738216991139	5.914653543076843E-4	5.560217375701451E-4	7.199484649812637E-4	0.0019183857559193055	1.1076130230480978E-4
90-94	0.07621042166384742	6.114023887225501E-4	5.914653543076843E-4	7.57607307764899E-4	0.001936107564288075	1.1076130230480978E-4
95-99	0.07625694141081543	6.44630779413993E-4	6.025414845381653E-4	8.04127054732919E-4	0.0019538293726568444	1.1076130230480978E-4
100-104	0.0763366895484749	6.667830398749549E-4	6.246937449991272E-4	8.240640891477849E-4	0.0019981338935787687	1.1076130230480978E-4
105-109	0.07642086813822657	7.066571087046865E-4	6.424155533678968E-4	8.683686100697087E-4	0.002031362284270211	1.1076130230480978E-4
110-114	0.07654492079680794	7.110875607968789E-4	6.579221356905701E-4	9.060274528533439E-4	0.0020845277093765203	1.1076130230480978E-4
115-119	0.07661359280423694	7.443159514883218E-4	7.066571087046865E-4	9.082426788994402E-4	0.002186428107496945	1.1076130230480978E-4
120-124	0.07714746228134613	7.753291161336685E-4	7.398854993961294E-4	9.193188091299213E-4	0.002310480766078332	1.1076130230480978E-4
125-129	0.07787627165051177	8.04127054732919E-4	7.642529859031875E-4	9.614081040057491E-4	0.002430102972567527	1.1076130230480978E-4
130-134	0.07870255096570564	8.284945412399772E-4	8.04127054732919E-4	0.001005712624927673	0.00255637085719501	1.1076130230480978E-4
135-139	0.07942914510882518	8.59507705885324E-4	8.307097672860735E-4	0.0010322953374808273	0.002678208289730301	1.1076130230480978E-4
140-144	0.0800671302101009	9.126731309916326E-4	8.37355445424362E-4	0.0010610932760800776	0.002804476174357784	1.1076130230480978E-4
145-149	0.0806475194341781	9.746994602823262E-4	8.572924798392279E-4	0.0010898912146793285	0.0029152374766625933	1.1076130230480978E-4
150-154	0.08119246504151777	0.0010012821728354808	8.92736096576767E-4	0.0011076130230480978	0.0030968860124424816	1.1076130230480978E-4
155-159	0.0818149435604708	0.00101900398120425	9.215340351760174E-4	0.001154132770016118	0.003256382287761408	1.1740698044309837E-4
160-164	0.08236431961990265	0.0010478019198035006	9.436862956369795E-4	0.0011696393523387913	0.0033161933910060047	1.2183743253529078E-4
165	0.08297129155653302	0.001063308502126174	9.636233300518452E-4	0.0011851459346614646	0.0033892958505271796	1.2183743253529077E-4
>>END_MODULE
