##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293771
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5208480
Total Bases	892.8 Mbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.14455061745461	41.0	40.0	41.0	38.0	41.0
2	40.21382533867846	41.0	40.0	41.0	38.0	41.0
3	40.23895416705066	41.0	40.0	41.0	38.0	41.0
4	40.24095302276288	41.0	40.0	41.0	38.0	41.0
5	40.25070308420115	41.0	40.0	41.0	38.0	41.0
6	40.26911363775996	41.0	40.0	41.0	39.0	41.0
7	40.23857401775566	41.0	40.0	41.0	39.0	41.0
8	40.24130821276073	41.0	40.0	41.0	39.0	41.0
9	40.1955447654594	41.0	40.0	41.0	38.0	41.0
10-14	40.157287961171015	41.0	40.0	41.0	38.0	41.0
15-19	40.06623506282064	41.0	40.0	41.0	38.0	41.0
20-24	39.92673973980893	41.0	40.0	41.0	38.0	41.0
25-29	39.69251854667773	41.0	39.6	41.0	36.8	41.0
30-34	39.35671044143396	40.0	38.6	41.0	35.4	41.0
35-39	38.881238211531965	40.0	37.6	41.0	35.0	41.0
40-44	38.39800079869751	39.6	36.4	41.0	35.0	41.0
45-49	37.888138305225326	38.8	35.0	41.0	34.8	41.0
50-54	37.29210479832888	37.0	35.0	40.0	34.8	41.0
55-59	36.58412557982367	35.8	35.0	38.8	34.0	41.0
60-64	35.74184368568181	35.0	34.8	36.6	33.4	39.4
65-69	35.50836885233312	35.0	35.0	36.0	34.0	38.4
70-74	35.246149894018984	35.0	35.0	35.6	34.0	37.0
75-79	35.08347179983412	35.0	35.0	35.0	34.0	36.4
80-84	35.0128187110251	35.0	35.0	35.0	34.0	36.0
85-89	35.90912016557614	36.2	35.8	36.2	34.6	37.0
90-94	40.06345325962976	41.0	40.0	41.0	38.0	41.0
95-99	40.01282383576466	41.0	40.0	41.0	38.0	41.0
100-104	39.897653759381214	41.0	40.0	41.0	37.8	41.0
105-109	39.75957694434567	41.0	40.0	41.0	37.0	41.0
110-114	39.565212786618154	41.0	39.2	41.0	36.0	41.0
115-119	39.274980592553085	40.0	38.6	41.0	35.0	41.0
120-124	38.89468829603568	40.0	37.8	41.0	35.0	41.0
125-129	37.88138288798511	39.0	36.0	40.2	34.2	40.6
130-134	38.14459818890113	39.0	35.4	41.0	35.0	41.0
135-139	37.67477517525535	38.0	35.0	40.8	35.0	41.0
140-144	36.97572502885128	36.4	35.0	39.4	34.4	41.0
145-149	36.28154487793321	35.4	35.0	38.0	34.0	40.4
150-154	35.68146442263054	35.0	35.0	36.6	34.0	39.0
155-159	35.23023626073379	35.0	35.0	35.8	34.0	37.0
160-164	34.94765453953879	35.0	35.0	35.0	34.0	36.2
165-169	34.785935273872035	35.0	35.0	35.0	34.0	36.0
170-174	34.69858693229874	35.0	35.0	35.0	34.0	36.0
175-176	34.42816056467127	35.0	34.5	35.0	32.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	34.0
30	333.0
31	2383.0
32	10764.0
33	36715.0
34	100677.0
35	262369.0
36	755434.0
37	1653216.0
38	1860836.0
39	517333.0
40	8384.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.997150800233467	27.91589868829294	28.33830599330323	20.748644518170366
2	20.155611617976838	26.056853439007156	30.496306024022363	23.291228918993642
3	20.976772494086564	25.386216324148315	31.272463367431573	22.364547814333548
4	22.24234325561392	26.094522778238566	28.846458083740362	22.81667588240715
5	22.566871716892454	26.374738887352933	28.12787991890148	22.930509476853132
6	22.42343255613922	27.169174116056894	27.91079163210764	22.49660169569625
7	22.6378713175437	26.96721500322551	27.65467084446902	22.740242834761773
8	22.942201947593155	26.942178908241942	27.296677725555245	22.81894141860965
9	22.479840567689614	27.47957177525881	27.604464258286487	22.43612339876509
10-14	22.76818956778177	27.104875126716433	27.391277301631185	22.73565800387061
15-19	22.549603723159155	27.25390133013854	27.61769268577397	22.57880226092833
20-24	22.55901913802107	27.068561269314657	27.671696925014594	22.70072266764968
25-29	22.644575768746353	27.112516511535034	27.447163087887443	22.795744631831166
30-34	22.539785119651032	27.221527201794	27.503114152305468	22.7355735262495
35-39	22.52427579639357	27.25027647221454	27.494032808036124	22.731414923355757
40-44	22.619251681872637	27.18466039996314	27.416125241913186	22.779962676251035
45-49	22.681941756520136	27.199770374466254	27.35031333517648	22.767974533837126
50-54	22.74817989125426	27.208294934414646	27.329397444167974	22.71412773016312
55-59	22.737382115319633	27.152720179399747	27.31113107854883	22.79876662673179
60-64	22.629181642244955	27.27640693638067	27.381443338555588	22.712968082818787
65-69	22.713171597087825	27.222901883082972	27.30503717015329	22.75888934967591
70-74	22.68345467391638	27.257798820385222	27.294304672380427	22.764441833317974
75-79	22.616536878321504	27.29486145670138	27.340552330046386	22.748049334930727
80-84	22.665683654348292	27.325027647221457	27.26875403188646	22.74053466654379
85-89	22.670184007618346	27.443423033207388	27.44598424108377	22.4404087180905
90-94	21.625575501667484	26.405256322487464	29.255693918615616	22.713474257229436
95-99	22.581276789888356	27.21458242134156	27.555865383540272	22.64827540522981
100-104	22.503425849870005	27.304942716343938	27.52655947132294	22.665071962463117
105-109	22.443100366222716	27.254836378359165	27.686612819659157	22.615450435758966
110-114	22.49554226213293	27.12977240313499	27.573788752128554	22.800896582603528
115-119	22.45411943030543	27.343857255234845	27.49435842901487	22.70766488544486
120-124	22.463231664038556	27.297665454993	27.492739180770954	22.74636370019749
125-129	22.469046026430647	27.32816648197528	27.44273886953638	22.760048622057695
130-134	22.569546879546202	27.2885785002383	27.37432886861465	22.76754575160085
135-139	22.666920478365533	27.353580459808626	27.3122296650657	22.667269396760144
140-144	22.61690851096147	27.35400536681687	27.34641891549795	22.68266720672371
145-149	22.515423543994554	27.491994502297857	27.38716242413653	22.605419529571062
150-154	22.521982831877025	27.5234985985481	27.40596345434288	22.548555115231988
155-159	22.46979090822489	27.56575805826055	27.395637622905756	22.568813410608808
160-164	22.377243127379685	27.727188925351648	27.446244382954553	22.449323564314117
165-169	22.38214361111919	27.764033409758742	27.437663911195926	22.416159067926145
170-174	22.336037164918494	27.809248837652106	27.482645498561187	22.37206849886821
175-176	22.236613703867985	27.956558271602557	27.464211773302484	22.34261625122698
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	3.5
10	9.5
11	24.5
12	52.5
13	93.5
14	182.5
15	328.0
16	535.0
17	907.5
18	1569.0
19	2509.5
20	3975.0
21	6272.5
22	9324.5
23	13214.5
24	18197.0
25	24233.0
26	31304.0
27	39283.0
28	47818.0
29	57212.0
30	66828.0
31	76835.0
32	87673.5
33	98674.0
34	110511.0
35	122940.5
36	135754.0
37	149167.0
38	162858.5
39	175808.0
40	187748.5
41	198113.0
42	205878.5
43	211717.5
44	214021.5
45	212775.0
46	209517.0
47	202873.5
48	193203.0
49	182072.5
50	169652.0
51	156756.5
52	144120.0
53	132288.0
54	121079.0
55	110725.0
56	102305.5
57	94559.0
58	87389.5
59	81182.0
60	75220.0
61	69730.0
62	64194.5
63	58241.5
64	51897.5
65	45428.0
66	39103.0
67	32976.0
68	27254.5
69	21968.5
70	17167.0
71	13100.5
72	9704.5
73	6940.5
74	4912.0
75	3285.5
76	2131.0
77	1378.0
78	850.5
79	510.0
80	289.5
81	171.5
82	104.5
83	59.0
84	33.0
85	13.0
86	3.0
87	2.0
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	365.0
92-93	779.0
94-95	1276.0
96-97	1665.0
98-99	2054.0
100-101	2383.0
102-103	2693.0
104-105	2947.0
106-107	3164.0
108-109	3616.0
110-111	3865.0
112-113	3940.0
114-115	4361.0
116-117	4481.0
118-119	4744.0
120-121	4932.0
122-123	5314.0
124-125	6026.0
126-127	5769.0
128-129	6056.0
130-131	6258.0
132-133	25404.0
134-135	38172.0
136-137	46793.0
138-139	42589.0
140-141	41521.0
142-143	39477.0
144-145	38957.0
146-147	38734.0
148-149	37998.0
150-151	37497.0
152-153	36497.0
154-155	40588.0
156-157	38300.0
158-159	38241.0
160-161	38640.0
162-163	40715.0
164-165	40916.0
166-167	41733.0
168-169	42690.0
170-171	43957.0
172-173	44132.0
174-175	83175.0
176-177	4215066.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.15933549189415
#Duplication Level	Percentage of total
1	92.60108416376414
2	6.657679811321839
3	0.5847079661486099
4	0.0987340725007805
5	0.0280359089245212
6	0.01362811057646353
7	0.005291637495510837
8	0.004273603478150889
9	0.004314145097740394
>10	0.002250580692248874
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.9199459343224895E-5	0.0	1.9199459343224895E-5	1.9199459343224895E-5	0.0	0.0
2	1.9199459343224895E-5	0.0	1.9199459343224895E-5	3.839891868644979E-5	0.0	0.0
3	1.9199459343224895E-5	3.839891868644979E-5	1.9199459343224895E-5	3.839891868644979E-5	0.0	0.0
4	1.9199459343224895E-5	3.839891868644979E-5	1.9199459343224895E-5	5.759837802967468E-5	1.9199459343224895E-5	0.0
5	3.839891868644979E-5	3.839891868644979E-5	1.9199459343224895E-5	7.679783737289958E-5	3.839891868644979E-5	0.0
6	3.839891868644979E-5	3.839891868644979E-5	1.9199459343224895E-5	7.679783737289958E-5	3.839891868644979E-5	0.0
7	3.839891868644979E-5	3.839891868644979E-5	1.9199459343224895E-5	9.599729671612447E-5	5.759837802967468E-5	0.0
8	3.839891868644979E-5	3.839891868644979E-5	1.9199459343224895E-5	1.1519675605934936E-4	7.679783737289958E-5	0.0
9	3.839891868644979E-5	3.839891868644979E-5	1.9199459343224895E-5	1.1519675605934936E-4	7.679783737289958E-5	0.0
10-14	5.37584861610297E-5	3.839891868644979E-5	6.143826989831967E-5	1.1519675605934935E-4	7.679783737289958E-5	0.0
15-19	6.527816176696465E-5	4.223881055509477E-5	8.063772924154456E-5	1.3055632353392926E-4	9.215740484747948E-5	0.0
20-24	8.447762111018952E-5	6.143826989831967E-5	9.599729671612447E-5	1.7279513408902407E-4	1.1903664792799434E-4	0.0
25-29	9.983718858476945E-5	1.1903664792799434E-4	1.228765397966393E-4	1.9199459343224894E-4	1.420759991398642E-4	0.0
30-34	2.2655362025005373E-4	1.420759991398642E-4	1.5743556661444415E-4	2.0735416090682883E-4	1.7279513408902407E-4	0.0
35-39	8.217368598900255E-4	1.7279513408902407E-4	1.996743771695389E-4	2.457530795932786E-4	2.4959297146192363E-4	1.5359567474579917E-5
40-44	0.0014245998832672871	1.8431480969495896E-4	2.303935121186987E-4	2.5727275519921356E-4	3.7630940312720796E-4	3.0719134949159833E-5
45-49	0.001793229502657205	2.303935121186987E-4	2.8031210641108346E-4	2.726323226737935E-4	5.798236721653918E-4	3.839891868644979E-5
50-54	0.002046662365987774	2.303935121186987E-4	3.340705925721132E-4	3.2255091696617823E-4	7.564586981230609E-4	4.6078702423739753E-5
55-59	0.0021503394464411885	2.303935121186987E-4	3.801492949958529E-4	3.839891868644979E-4	9.2541394034344E-4	5.7598378029674675E-5
60-64	0.002296255337449697	2.303935121186987E-4	4.3390778115688265E-4	4.031886462077227E-4	0.0011519675605934938	6.143826989831967E-5
65-69	0.0024498510121954966	2.457530795932786E-4	4.646269161060424E-4	4.031886462077227E-4	0.0013977206401867724	8.063772924154456E-5
70-74	0.01080545571836697	2.8031210641108346E-4	4.991859429238474E-4	4.223881055509477E-4	0.0015436365311952815	1.1519675605934935E-4
75-79	0.06598086197892668	3.1103124136024334E-4	5.183854022670722E-4	4.7230669984333246E-4	0.001616594476699536	1.1519675605934935E-4
80-84	0.07770021196203117	3.263908088348232E-4	5.299050778730071E-4	5.145455103984272E-4	0.0017663502595766903	1.1519675605934935E-4
85-89	0.07775781034006082	3.263908088348232E-4	5.37584861610297E-4	5.52944429084877E-4	0.001989063987958099	1.1519675605934935E-4
90-94	0.0778499677449083	3.3023070070346816E-4	5.49104537216232E-4	6.259023745891315E-4	0.0020351426903818387	1.1519675605934935E-4
95-99	0.07794596504162442	3.4559026817804813E-4	5.759837802967469E-4	6.681411851442263E-4	0.002088901176542868	1.1519675605934935E-4
100-104	0.07804964212207785	3.686296193899179E-4	5.759837802967469E-4	6.758209688815163E-4	0.0020927410684115134	1.1519675605934935E-4
105-109	0.07808420114889565	4.031886462077227E-4	5.990231315086167E-4	7.833379412035757E-4	0.0021042607440174485	1.1519675605934935E-4
110-114	0.07813795963505668	4.185482136823027E-4	6.412619420637115E-4	8.409363192332503E-4	0.0021426596627038986	1.1519675605934935E-4
115-119	0.07819939790495499	4.223881055509477E-4	6.681411851442263E-4	8.639756704451202E-4	0.0022156176082081527	1.1519675605934935E-4
120-124	0.07867554449666697	4.415875648941726E-4	6.911805363560962E-4	8.946948053942802E-4	0.0022962553374496976	1.305563235339293E-4
125-129	0.07927456762817559	4.799864835806224E-4	7.41099130648481E-4	9.215740484747948E-4	0.0024460111203268515	1.3439621540257426E-4
130-134	0.07981599238165453	5.183854022670721E-4	7.871778330722208E-4	9.446133996866647E-4	0.0025765674438607807	1.3439621540257426E-4
135-139	0.0803497373513962	5.37584861610297E-4	8.370964273646054E-4	0.0010022117777163395	0.0026802445243141954	1.420759991398642E-4
140-144	0.08107931680643873	5.491045372162319E-4	8.524559948391854E-4	0.0010559702638773694	0.0028261604153227045	1.5743556661444415E-4
145-149	0.08146714588517187	5.798236721653918E-4	8.870150216569901E-4	0.001109728750038399	0.0030066353331490185	1.7279513408902407E-4
150-154	0.08189337388259146	5.951832396399717E-4	9.522931834239547E-4	0.0011519675605934938	0.003133351764814303	1.7279513408902407E-4
155-159	0.08242711885233311	6.105428071145516E-4	9.599729671612448E-4	0.0011673271280680738	0.0032907873314287467	1.7279513408902407E-4
160-164	0.0830146223082358	6.681411851442263E-4	9.791724265044697E-4	0.0012095659386231685	0.0034175037630940312	1.7663502595766903E-4
165	0.08369044327711732	6.911805363560963E-4	9.983718858476945E-4	0.0012287653979663933	0.003475102141123706	1.9199459343224894E-4
>>END_MODULE
