##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293776
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7708958
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.89488358867697	41.0	40.0	41.0	38.0	41.0
2	40.03446445031871	41.0	40.0	41.0	38.0	41.0
3	40.08859070188215	41.0	40.0	41.0	38.0	41.0
4	40.11653741011431	41.0	40.0	41.0	38.0	41.0
5	40.11238665459067	41.0	40.0	41.0	38.0	41.0
6	40.132681874774775	41.0	40.0	41.0	38.0	41.0
7	40.1193665862494	41.0	40.0	41.0	38.0	41.0
8	40.09302113203886	41.0	40.0	41.0	38.0	41.0
9	40.06746488954798	41.0	40.0	41.0	38.0	41.0
10-14	40.00284972365915	41.0	40.0	41.0	38.0	41.0
15-19	39.83831804505875	41.0	40.0	41.0	38.0	41.0
20-24	39.608591796712346	40.0	39.4	41.0	37.0	41.0
25-29	39.27949909702452	40.0	38.6	41.0	35.6	41.0
30-34	39.04336254004756	40.0	38.0	41.0	35.0	41.0
35-39	38.52666726164547	39.8	36.6	41.0	35.0	41.0
40-44	37.96099475441429	39.0	35.2	41.0	34.0	41.0
45-49	37.409856195869786	37.4	35.0	40.2	34.0	41.0
50-54	36.75241234937329	36.2	35.0	39.4	34.0	41.0
55-59	36.13973387843078	35.0	35.0	38.0	33.2	40.4
60-64	35.23774569792701	34.8	34.4	36.4	32.2	39.4
65-69	35.34081153380262	35.0	35.0	36.0	33.0	38.8
70-74	35.17381949674651	35.0	35.0	35.4	33.0	37.2
75-79	35.04211702800819	35.0	35.0	35.0	33.0	37.0
80-84	34.98244515017464	35.0	35.0	35.0	33.0	37.0
85-89	35.787828030714394	36.2	35.6	36.2	33.4	38.0
90-94	39.78277950640149	41.0	40.0	41.0	37.0	41.0
95-99	39.717776717306414	41.0	40.0	41.0	37.0	41.0
100-104	39.55840350912502	41.0	39.2	41.0	36.0	41.0
105-109	39.36138980651209	40.2	39.0	41.0	35.2	41.0
110-114	39.09485804694923	40.0	38.0	41.0	35.0	41.0
115-119	38.70348893913622	40.0	37.6	41.0	35.0	41.0
120-124	38.43450718058739	40.0	36.6	41.0	34.8	41.0
125-129	37.50054233858487	38.4	35.2	40.0	33.6	40.6
130-134	37.46278865767938	37.8	35.0	40.6	34.0	41.0
135-139	36.80712221959067	36.4	35.0	39.6	33.0	41.0
140-144	36.141323134269406	35.2	35.0	38.6	33.0	40.6
145-149	35.78110946829395	35.0	35.0	37.0	33.0	39.4
150-154	35.31649530199202	35.0	35.0	36.0	33.0	38.2
155-159	34.93441849655202	35.0	35.0	35.2	33.0	36.6
160-164	34.685714040419114	35.0	35.0	35.0	33.0	36.0
165-169	34.538830478654575	35.0	35.0	35.0	33.0	36.0
170-174	34.44401034091446	35.0	34.4	35.0	33.0	35.0
175-176	34.02141246078788	35.0	33.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	5.0
30	160.0
31	2078.0
32	13990.0
33	61764.0
34	209016.0
35	614934.0
36	1651444.0
37	2618931.0
38	2097442.0
39	435571.0
40	3623.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.97556193716453	26.30642169797786	26.723456010526974	22.994560354330638
2	21.28177374944837	25.405521731990238	28.893671492308037	24.419033026253352
3	22.363554192408365	24.556846722994212	29.268832441427232	23.810766643170194
4	23.665948108680837	25.258990903829027	26.887018971954447	24.188042015535693
5	23.46070636264979	25.887065411434335	26.891105127307736	23.761123098608138
6	23.414785759631844	25.893849726512975	26.703621942161316	23.987742571693865
7	23.936257014242393	25.92616278360837	25.926850295461463	24.210729906687778
8	23.866273496366176	26.292230415576267	26.18487479111963	23.656621296937928
9	23.470409360123636	26.259307678158315	26.300065456317185	23.97021750540086
10-14	23.95885669632653	26.19624857211571	25.926621989638548	23.918272741919207
15-19	23.752089452296925	26.140990779817454	26.13012809253858	23.976791675347044
20-24	23.741141150334457	25.996698905351412	26.256230738317683	24.005929205996452
25-29	23.8939451998571	26.01967477316649	25.947153947394707	24.139226079581704
30-34	23.885923363442892	25.955933344039494	25.94369303867008	24.214450253847534
35-39	23.818806640274858	26.018927590473318	26.00362072280067	24.158645046451156
40-44	24.03771041429983	25.9556557449139	25.809249447201555	24.19738439358471
45-49	24.133085171822184	25.830847696926096	25.776093215191985	24.25997391605973
50-54	24.08134277031993	25.924419357324297	25.866572369443446	24.127665502912325
55-59	24.13762534443695	25.900519888680158	25.766200827660494	24.195653939222396
60-64	24.026035684718998	25.8700851658551	25.83648010535276	24.267399044073144
65-69	24.015377953803874	25.91930842015224	25.88335803619633	24.18195558984755
70-74	24.0325449950564	25.928593721745536	25.831945121506696	24.20691616169137
75-79	23.96309073158785	25.886772246002636	25.84150542784122	24.308631594568293
80-84	23.936197343402313	25.9372252384823	25.89029801433605	24.236279403779342
85-89	23.98885296819622	25.946993095564924	26.011305289249208	24.052848646989645
90-94	22.942640233713902	25.51666978133667	27.501326091729894	24.03936389321953
95-99	23.787979736710664	26.239501277204052	26.06818299723678	23.904335988848505
100-104	23.785334942544885	26.189988350698606	26.000267287587747	24.02440941916876
105-109	23.677468118180027	26.166338489840346	26.256704910967937	23.899488481011684
110-114	23.82161742199516	26.028565020206155	26.059702074431463	24.090115483367217
115-119	23.780787250660843	26.067050418605536	25.98312505295906	24.169037277774564
120-124	23.81191548787964	26.090129126187207	26.004763160765126	24.09319222516802
125-129	23.85277116023832	26.094919780612518	25.93938829271104	24.11292076643812
130-134	23.961331965389434	25.986551818977873	25.850941532847514	24.201174682785183
135-139	23.993866284111316	26.109074596363392	25.862909823126973	24.034149296398326
140-144	23.98516753935123	26.14349268563201	25.839342466769665	24.031997308247092
145-149	23.803854550743615	26.21481580064055	25.998500620324094	23.98282902829174
150-154	23.76060855333168	26.314393702064407	26.10810687656029	23.816890868043618
155-159	23.73963091435232	26.370451817188044	26.076692517653516	23.81322475080612
160-164	23.605213014082256	26.41882386593366	26.177843889411328	23.798119230572752
165-169	23.546247969598273	26.55303043924937	26.260206121016182	23.640515470136176
170-174	23.474378772213804	26.652402047505376	26.280799492997055	23.59241968728377
175-176	23.3771415625921	26.609845450168777	26.31558522413736	23.697427763101764
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	1.0
9	1.5
10	3.0
11	8.0
12	13.5
13	32.5
14	63.5
15	111.0
16	188.0
17	354.0
18	655.5
19	1181.5
20	2096.5
21	3676.5
22	6085.5
23	9566.0
24	14334.5
25	20496.0
26	27906.5
27	36645.5
28	46670.5
29	57619.5
30	69864.0
31	82886.5
32	96572.0
33	111428.5
34	127262.0
35	143596.0
36	160599.0
37	178046.5
38	196012.0
39	213776.5
40	230097.5
41	245500.5
42	259566.0
43	270473.5
44	278421.5
45	283646.0
46	284986.0
47	283071.5
48	277918.0
49	269641.5
50	259327.5
51	248432.0
52	236688.5
53	223765.0
54	211859.0
55	201780.0
56	192799.5
57	184646.0
58	177676.0
59	171093.5
60	163974.0
61	156577.5
62	148336.0
63	138373.0
64	126495.5
65	113360.0
66	99661.5
67	85298.5
68	70664.0
69	56661.5
70	44354.5
71	33927.0
72	24761.0
73	17326.5
74	11857.0
75	7829.0
76	4876.0
77	2845.0
78	1636.0
79	921.0
80	478.0
81	243.5
82	110.5
83	49.5
84	24.5
85	9.0
86	5.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	990.0
92-93	2456.0
94-95	3949.0
96-97	5224.0
98-99	7748.0
100-101	8071.0
102-103	9577.0
104-105	10607.0
106-107	11216.0
108-109	12367.0
110-111	13809.0
112-113	13221.0
114-115	13994.0
116-117	14529.0
118-119	14768.0
120-121	15427.0
122-123	16289.0
124-125	16993.0
126-127	17640.0
128-129	18497.0
130-131	19581.0
132-133	57168.0
134-135	100317.0
136-137	102153.0
138-139	108135.0
140-141	106644.0
142-143	106386.0
144-145	92195.0
146-147	87575.0
148-149	85695.0
150-151	86144.0
152-153	79012.0
154-155	77575.0
156-157	84312.0
158-159	84036.0
160-161	84848.0
162-163	86839.0
164-165	91771.0
166-167	92181.0
168-169	95919.0
170-171	100485.0
172-173	106392.0
174-175	231025.0
176-177	5315198.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.73116054534269
#Duplication Level	Percentage of total
1	94.0041551370936
2	4.8164902220712635
3	0.6247199381326666
4	0.22725975875678225
5	0.14975516979430892
6	0.06813621556685018
7	0.05195208681545887
8	0.016731917377976774
9	0.012629714336779178
>10	0.01868852964847251
>50	0.005658539642397941
>100	0.0038227707634443248
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	3.8915765269443676E-5	3.8915765269443676E-5	1.2971921756481227E-5	0.0	0.0
2	0.0	3.8915765269443676E-5	3.8915765269443676E-5	2.5943843512962453E-5	0.0	0.0
3	1.2971921756481227E-5	6.485960878240613E-5	6.485960878240613E-5	3.8915765269443676E-5	0.0	0.0
4	1.2971921756481227E-5	9.080345229536859E-5	7.783153053888735E-5	3.8915765269443676E-5	0.0	0.0
5	1.2971921756481227E-5	9.080345229536859E-5	1.0377537405184981E-4	5.1887687025924906E-5	0.0	0.0
6	1.2971921756481227E-5	9.080345229536859E-5	1.0377537405184981E-4	5.1887687025924906E-5	0.0	0.0
7	3.8915765269443676E-5	1.0377537405184981E-4	1.2971921756481226E-4	6.485960878240613E-5	0.0	0.0
8	3.8915765269443676E-5	1.1674729580833104E-4	1.2971921756481226E-4	9.080345229536859E-5	0.0	0.0
9	3.8915765269443676E-5	1.2971921756481226E-4	1.2971921756481226E-4	9.080345229536859E-5	0.0	0.0
10-14	4.6698918323332415E-5	1.323136019161085E-4	1.4009675496999723E-4	9.858660534925731E-5	7.783153053888736E-6	0.0
15-19	8.561468359277609E-5	1.452855236725897E-4	1.7122936718555218E-4	1.8420128894203338E-4	1.2971921756481227E-5	0.0
20-24	1.1415291145703478E-4	1.6344621413166344E-4	2.023619794011071E-4	2.1273951680629214E-4	2.5943843512962453E-5	0.0
25-29	1.5306867672647845E-4	1.8939005764462592E-4	2.179282855088846E-4	2.4906089772443955E-4	3.8915765269443676E-5	0.0
30-34	2.1533390115758836E-4	1.997675950498109E-4	2.5684405077832826E-4	2.905710473451795E-4	3.8915765269443676E-5	0.0
35-39	2.9575981604777193E-4	2.3868336031925458E-4	2.775991255886982E-4	3.113261221555494E-4	5.707645572851739E-5	7.783153053888736E-6
40-44	3.7099696223536307E-4	2.5165528207573575E-4	2.905710473451795E-4	4.047239588022142E-4	9.858660534925731E-5	1.8160690459073717E-5
45-49	4.4623410842295415E-4	2.698159725348095E-4	3.320811969659194E-4	4.1769588055869545E-4	1.608518297803672E-4	2.5943843512962453E-5
50-54	5.059049485027677E-4	2.879766629938832E-4	3.969408057483255E-4	4.358565710177693E-4	1.8420128894203343E-4	2.5943843512962453E-5
55-59	5.448207137722115E-4	3.165148908581419E-4	4.280734179638805E-4	4.618004145307317E-4	2.0495636375240334E-4	2.5943843512962453E-5
60-64	5.785477103390626E-4	3.346755813172156E-4	4.540172614768429E-4	5.214712546105453E-4	2.672215881835133E-4	2.5943843512962453E-5
65-69	6.174634756085063E-4	3.554306561275856E-4	5.162824859079528E-4	5.993027851494326E-4	2.85382278642587E-4	2.5943843512962453E-5
70-74	0.0015177148455083034	4.33262186666473E-4	5.889252477442477E-4	6.615680095805425E-4	3.217036595607344E-4	2.5943843512962453E-5
75-79	0.007217577265306155	4.7477233628721285E-4	6.25246628662395E-4	7.004837748499862E-4	3.398643500198081E-4	4.1510149620739925E-5
80-84	0.008496608750495203	5.370375607183228E-4	6.511904721753575E-4	7.238332340116525E-4	4.7217795193591665E-4	5.1887687025924906E-5
85-89	0.008545902053169832	5.759533259877663E-4	6.875118530935049E-4	7.549658462272074E-4	6.226522443110988E-4	5.1887687025924906E-5
90-94	0.008597789740195757	5.915196320955439E-4	7.316163870655413E-4	8.353917611173911E-4	6.25246628662395E-4	5.1887687025924906E-5
95-99	0.008634111121113905	6.408129347701727E-4	7.549658462272074E-4	9.158176760075745E-4	6.3562416606758E-4	5.1887687025924906E-5
100-104	0.008683404423788533	6.589736252292463E-4	7.964759958479473E-4	0.0010066211283029432	6.3562416606758E-4	5.1887687025924906E-5
105-109	0.008704159498598903	6.693511626344312E-4	8.66524373332946E-4	0.0010299705874646094	6.460017034727651E-4	5.1887687025924906E-5
110-114	0.008711942651652791	6.745399313370237E-4	9.521390569257219E-4	0.0010662919683827568	6.771343156883198E-4	5.1887687025924906E-5
115-119	0.008717131420355384	6.9788939049869E-4	9.936492065464619E-4	0.001100018964949608	6.9788939049869E-4	5.1887687025924906E-5
120-124	0.00876901910738131	7.679377679836888E-4	0.0010221874344107206	0.001120774039759978	7.108613122551712E-4	5.1887687025924906E-5
125-129	0.00885982255967668	8.224198393609097E-4	0.0011026133493009043	0.0011363403458677554	7.186444653090599E-4	5.448207137722115E-5
130-134	0.008924682168459083	8.457692985225759E-4	0.0011363403458677554	0.0011778504954884955	7.368051557681337E-4	6.745399313370237E-5
135-139	0.009062184539077785	9.054401386023895E-4	0.0011545010363268292	0.0012011999546501615	7.393995401194299E-4	7.783153053888735E-5
140-144	0.009140016069616671	9.495446725744257E-4	0.0011674729580833103	0.001247898872973494	7.49777077524615E-4	7.783153053888735E-5
145-149	0.009173743066183524	9.599222099796106E-4	0.0012037943390014578	0.0012971921756481225	7.835040740914661E-4	8.042591489018361E-5
150-154	0.009220441984506856	9.806772847899808E-4	0.001237521335568309	0.0013568630157279361	7.912872271453548E-4	9.080345229536859E-5
155-159	0.009259357749776298	9.858660534925732E-4	0.001289409022594234	0.001398373165348676	7.912872271453548E-4	9.599222099796107E-5
160-164	0.009316434205504816	9.858660534925732E-4	0.0013023809443507151	0.0014139394714564536	7.912872271453548E-4	1.0377537405184981E-4
165	0.009352755586422965	9.988379752490545E-4	0.0013101640974046039	0.001426911393212935	7.912872271453548E-4	1.0377537405184981E-4
>>END_MODULE
