##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293777
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7673199
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.89801254991562	41.0	40.0	41.0	38.0	41.0
2	40.031429525026006	41.0	40.0	41.0	38.0	41.0
3	40.08587513499911	41.0	40.0	41.0	38.0	41.0
4	40.10999388911978	41.0	40.0	41.0	38.0	41.0
5	40.10865077264385	41.0	40.0	41.0	38.0	41.0
6	40.126794313558136	41.0	40.0	41.0	38.0	41.0
7	40.113543907827754	41.0	40.0	41.0	38.0	41.0
8	40.092397968565656	41.0	40.0	41.0	38.0	41.0
9	40.07189830473574	41.0	40.0	41.0	38.0	41.0
10-14	40.00300002645572	41.0	40.0	41.0	38.0	41.0
15-19	39.8420366525096	41.0	40.0	41.0	38.0	41.0
20-24	39.61197985351351	40.0	39.4	41.0	37.0	41.0
25-29	39.27376339385959	40.0	38.6	41.0	35.6	41.0
30-34	39.0265122278205	40.0	38.0	41.0	35.0	41.0
35-39	38.504298428856075	39.8	36.6	41.0	35.0	41.0
40-44	37.92446845181521	39.0	35.2	41.0	34.0	41.0
45-49	37.366584210835654	37.4	35.0	40.2	34.0	41.0
50-54	36.69572828751085	36.2	35.0	39.2	34.0	41.0
55-59	36.08130744426151	35.0	35.0	38.0	33.0	40.4
60-64	35.21346530957949	34.8	34.4	36.4	32.4	39.2
65-69	35.29557810243159	35.0	35.0	36.0	33.0	38.6
70-74	35.13792182374001	35.0	35.0	35.2	33.0	37.0
75-79	35.013331753809595	35.0	35.0	35.0	33.0	37.0
80-84	34.95220387220506	35.0	35.0	35.0	33.0	37.0
85-89	35.77838903956486	36.2	35.6	36.2	33.4	38.0
90-94	39.813209594370235	41.0	40.0	41.0	37.2	41.0
95-99	39.74745263592157	41.0	40.0	41.0	37.0	41.0
100-104	39.59139058641405	41.0	39.4	41.0	36.2	41.0
105-109	39.39458967478181	40.2	39.0	41.0	35.4	41.0
110-114	39.13013293269061	40.0	38.0	41.0	35.0	41.0
115-119	38.73042770246358	40.0	37.6	41.0	35.0	41.0
120-124	38.46937571641938	40.0	36.6	41.0	34.8	41.0
125-129	37.55694344491233	38.8	35.2	40.2	33.6	40.6
130-134	37.49045702014997	38.2	35.0	40.6	34.0	41.0
135-139	36.84203699369205	36.6	35.0	39.8	33.2	41.0
140-144	36.18174841231447	35.2	35.0	38.6	33.0	40.8
145-149	35.814845113668056	35.0	35.0	37.0	33.4	39.6
150-154	35.349685029765546	35.0	35.0	36.0	33.0	38.2
155-159	34.957834662029235	35.0	35.0	35.2	33.0	36.8
160-164	34.70111038343977	35.0	35.0	35.0	33.0	36.0
165-169	34.54946061648767	35.0	35.0	35.0	33.0	36.0
170-174	34.452309470725176	35.0	34.4	35.0	33.0	35.0
175-176	34.03726583194869	35.0	33.5	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	6.0
30	164.0
31	2022.0
32	13884.0
33	61664.0
34	206360.0
35	606215.0
36	1650126.0
37	2623017.0
38	2082216.0
39	424667.0
40	2858.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.98996819970393	26.262084431799565	26.713200061669195	23.034747306827306
2	21.298509265822506	25.312975722381235	28.90127051311976	24.487244498676496
3	22.42158713725527	24.50479910660469	29.201653704015758	23.871960052124283
4	23.73081944049672	25.14601797763879	26.857142633730728	24.266019948133756
5	23.52546832162179	25.840982359508725	26.84404509774867	23.789504221120815
6	23.446453037383755	25.86506618686678	26.579592162278082	24.108888613471382
7	23.954090073774967	25.872651028599673	25.899289201283587	24.273969696341773
8	23.919019955040916	26.266528471371586	26.09841084533322	23.716040728254278
9	23.4946597892222	26.2231436979544	26.23633246055524	24.04586405226816
10-14	23.98407756660553	26.139475856158562	25.892455024299515	23.983991552936395
15-19	23.792897851339447	26.085276818703644	26.094258730941295	24.02756659901561
20-24	23.776523976505757	25.947297861035533	26.2095040152093	24.066674147249405
25-29	23.94180054498782	25.974280088395986	25.88733851422334	24.196580852392856
30-34	23.917953906838598	25.92348771353382	25.887320268899582	24.271238110728003
35-39	23.8599702679417	25.970221807097666	25.961284204932	24.208523720028634
40-44	24.07092530768458	25.904069476107683	25.772054132832995	24.252951083374743
45-49	24.17616954805942	25.785420135721747	25.736997046473054	24.301413269745773
50-54	24.130717840108147	25.85004246599104	25.816723898337578	24.202515795563233
55-59	24.168266716398207	25.85555776671503	25.72818455509886	24.247990961787906
60-64	24.06973675516561	25.82578921776954	25.793028435728043	24.31144559133681
65-69	24.065639376744954	25.865246033629518	25.840625272458073	24.22848931716746
70-74	24.09934630914694	25.870868199821224	25.76677602131784	24.263009469714
75-79	24.021748947212238	25.815511887545206	25.807788902646735	24.354950262595825
80-84	23.98467705581466	25.87511936025639	25.846216160951908	24.29398742297704
85-89	24.043072517733478	25.888975380411743	25.960126930110896	24.107825171743887
90-94	22.98547201445525	25.469668751001386	27.456924470460788	24.08793476408258
95-99	23.851341390172625	26.206805408042396	25.990500519172965	23.951352682612015
100-104	23.831043820386462	26.14180016521151	25.962852413561073	24.064303600840955
105-109	23.736913430882893	26.10986103072111	26.196959005685322	23.95626653271068
110-114	23.86704167290337	25.978173979734198	26.01637630929946	24.138408038062973
115-119	23.84024794425084	26.029912273948653	25.925266627686476	24.204573154114033
120-124	23.86597106640836	26.024085366639444	25.949530196677223	24.160413370274973
125-129	23.89927643981969	26.035107689438565	25.893864823266387	24.171751047475354
130-134	24.040812119730052	25.933800464649686	25.782035413925147	24.24335200169511
135-139	24.050902916227095	26.041784578271898	25.82315782638383	24.084154679117177
140-144	24.031029999700714	26.09436072882041	25.790061923990677	24.084547347488208
145-149	23.870691150124195	26.164068594059696	25.941095388980102	24.024144866836007
150-154	23.826842404515734	26.246766234264	26.059876124776316	23.86651523644395
155-159	23.81178933420747	26.30723866998877	26.019756377487514	23.861215618316248
160-164	23.685623521096836	26.356055599021655	26.118422164604087	23.839898715277418
165-169	23.607012643979846	26.479205632505977	26.20627075390119	23.707510969612983
170-174	23.552492999177165	26.561403494021558	26.23651192550093	23.649591581300346
175-176	23.432131345784697	26.567091830113505	26.269760120373007	23.73101670372879
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.5
8	0.5
9	1.0
10	2.0
11	6.0
12	14.0
13	24.5
14	49.5
15	99.5
16	194.0
17	334.5
18	612.5
19	1109.5
20	2043.5
21	3576.0
22	5923.0
23	9382.5
24	14011.0
25	19810.5
26	27018.5
27	35598.5
28	45526.5
29	56752.0
30	68858.5
31	81333.5
32	94904.0
33	109449.0
34	124598.0
35	140982.5
36	158185.5
37	175617.0
38	193281.0
39	211233.0
40	228108.0
41	243373.5
42	256388.5
43	267531.0
44	276323.0
45	281918.0
46	283268.0
47	280657.5
48	276110.0
49	268363.0
50	258275.5
51	247175.0
52	235100.5
53	223365.5
54	212190.5
55	201895.5
56	193352.0
57	185567.0
58	178201.0
59	171642.0
60	165325.5
61	158137.0
62	149802.0
63	139512.5
64	127397.0
65	114589.5
66	100448.5
67	85686.0
68	71740.0
69	58163.5
70	45193.5
71	34169.5
72	25078.5
73	17756.5
74	12120.0
75	7869.0
76	4918.0
77	2975.0
78	1731.0
79	970.5
80	515.5
81	260.0
82	117.0
83	57.0
84	24.5
85	10.5
86	6.0
87	2.0
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	954.0
92-93	2344.0
94-95	3693.0
96-97	4955.0
98-99	6449.0
100-101	7644.0
102-103	8622.0
104-105	9505.0
106-107	10584.0
108-109	11495.0
110-111	11967.0
112-113	12644.0
114-115	13064.0
116-117	13562.0
118-119	13959.0
120-121	14599.0
122-123	15109.0
124-125	15909.0
126-127	16684.0
128-129	17636.0
130-131	18490.0
132-133	54425.0
134-135	95887.0
136-137	98227.0
138-139	97294.0
140-141	100548.0
142-143	102228.0
144-145	87749.0
146-147	83857.0
148-149	83228.0
150-151	82061.0
152-153	75808.0
154-155	75914.0
156-157	78468.0
158-159	79415.0
160-161	82769.0
162-163	84727.0
164-165	86891.0
166-167	90580.0
168-169	93938.0
170-171	98283.0
172-173	107923.0
174-175	204904.0
176-177	5398207.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.65366051125132
#Duplication Level	Percentage of total
1	93.87096714359767
2	4.909026512492059
3	0.6419519569067291
4	0.23338891069510817
5	0.14771766855080762
6	0.08259978366611964
7	0.047803476129194944
8	0.023010751659651746
9	0.010527090517867746
>10	0.02318654017392183
>50	0.0029785621647937474
>100	0.006841603446051447
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.3032374111501604E-5	0.0	0.0	1.3032374111501604E-5	0.0
2	0.0	2.606474822300321E-5	0.0	0.0	1.3032374111501604E-5	0.0
3	1.3032374111501604E-5	3.909712233450481E-5	0.0	0.0	1.3032374111501604E-5	0.0
4	1.3032374111501604E-5	5.212949644600642E-5	1.3032374111501604E-5	0.0	1.3032374111501604E-5	0.0
5	1.3032374111501604E-5	5.212949644600642E-5	1.3032374111501604E-5	1.3032374111501604E-5	1.3032374111501604E-5	0.0
6	1.3032374111501604E-5	6.516187055750802E-5	2.606474822300321E-5	2.606474822300321E-5	1.3032374111501604E-5	0.0
7	2.606474822300321E-5	6.516187055750802E-5	2.606474822300321E-5	3.909712233450481E-5	1.3032374111501604E-5	0.0
8	2.606474822300321E-5	9.122661878051123E-5	2.606474822300321E-5	3.909712233450481E-5	2.606474822300321E-5	0.0
9	2.606474822300321E-5	9.122661878051123E-5	3.909712233450481E-5	6.516187055750802E-5	2.606474822300321E-5	0.0
10-14	3.649064751220449E-5	1.1729136700351444E-4	6.776834537980834E-5	6.776834537980834E-5	2.606474822300321E-5	0.0
15-19	5.734244609060706E-5	1.4074964040421733E-4	9.904604324741219E-5	1.0947194253661348E-4	2.606474822300321E-5	0.0
20-24	8.080071949130994E-5	1.5638848933801925E-4	1.2250431664811507E-4	1.7463381309412149E-4	2.606474822300321E-5	0.0
25-29	1.0425899289201283E-4	1.6160143898261988E-4	1.4856906487111828E-4	2.2155035989552727E-4	3.127769786760385E-5	0.0
30-34	1.2771726629271573E-4	1.7463381309412149E-4	1.876661872056231E-4	2.3458273400702888E-4	4.691654680140577E-5	2.606474822300321E-6
35-39	2.1633741025092664E-4	2.1112446060632598E-4	2.1112446060632598E-4	2.476151081185305E-4	5.734244609060706E-5	2.3458273400702886E-5
40-44	3.0756402903143787E-4	2.397956836516295E-4	2.606474822300321E-4	2.788928059861343E-4	7.558776984670931E-5	4.1703597156805135E-5
45-49	3.518741010105433E-4	2.7107338151923335E-4	2.997446045645369E-4	3.2320287796523976E-4	8.862014395821091E-5	6.776834537980834E-5
50-54	3.935776981673484E-4	2.997446045645369E-4	3.2320287796523976E-4	3.596935254774443E-4	9.643956842511187E-5	9.904604324741219E-5
55-59	4.352812953241535E-4	3.388417268990417E-4	3.518741010105433E-4	4.013971226342494E-4	1.3553669075961667E-4	1.4074964040421733E-4
60-64	5.212949644600642E-4	3.4926762618824297E-4	3.8315179887814715E-4	4.6916546801405775E-4	2.0330503613942504E-4	1.6942086344952085E-4
65-69	5.682115112614699E-4	3.909712233450481E-4	4.0661007227885007E-4	5.343273385715658E-4	2.4240215847392985E-4	2.0330503613942504E-4
70-74	0.0014257417277982755	4.14429496745751E-4	4.30068345679553E-4	5.656050364391697E-4	2.919251800976359E-4	2.1894388507322696E-4
75-79	0.007076579142545371	4.1703597156805133E-4	4.4570719461335483E-4	6.02095683951374E-4	3.518741010105433E-4	2.4240215847392985E-4
80-84	0.008296409359381921	4.326748205018533E-4	4.561330939025561E-4	6.516187055750801E-4	4.7698489248095867E-4	2.476151081185305E-4
85-89	0.008322474107604925	4.4310071979105454E-4	4.665589931917574E-4	6.724705041534827E-4	6.125215832405754E-4	2.476151081185305E-4
90-94	0.008340719431361027	4.4310071979105454E-4	4.717719428363581E-4	6.90715827909585E-4	6.203410077074763E-4	2.476151081185305E-4
95-99	0.00837199712922863	4.665589931917574E-4	4.95230216237061E-4	7.402388495332912E-4	6.307669069966777E-4	2.476151081185305E-4
100-104	0.008392848927807034	5.239014392823645E-4	5.134755399931632E-4	7.793359718677959E-4	6.385863314635786E-4	2.476151081185305E-4
105-109	0.008418913676030036	5.395402882161665E-4	5.265079141046649E-4	8.184330942023008E-4	6.490122307527799E-4	2.476151081185305E-4
110-114	0.008418913676030036	5.551791371499684E-4	5.629985616168693E-4	8.783820151152082E-4	6.516187055750802E-4	2.476151081185305E-4
115-119	0.008431946050141538	5.708179860837702E-4	6.09915108418275E-4	9.670021590734189E-4	6.516187055750802E-4	2.476151081185305E-4
120-124	0.008486682021409845	5.968827343067736E-4	6.333733818189779E-4	0.0010321640296309272	6.646510796865818E-4	2.502215829408308E-4
125-129	0.008572695690545754	6.125215832405754E-4	6.542251803973804E-4	0.0010842935260769334	6.90715827909585E-4	2.606474822300321E-4
130-134	0.008637857561103263	6.464057559304795E-4	7.115676264879877E-4	0.0010999323750107354	6.90715827909585E-4	2.7107338151923335E-4
135-139	0.008744723028817576	6.82896403442684E-4	7.532712236447928E-4	0.0011259971232337386	6.90715827909585E-4	2.736798563415337E-4
140-144	0.008880259719577194	7.193870509548886E-4	8.027942452684988E-4	0.0011416359721675407	7.089611516656873E-4	2.736798563415337E-4
145-149	0.0089402086404901	7.298129502440898E-4	8.262525186692017E-4	0.001201584893080448	7.298129502440898E-4	2.736798563415337E-4
150-154	0.008966273388713104	7.584841732893933E-4	8.36678417958403E-4	0.001227649641303451	7.584841732893933E-4	2.736798563415337E-4
155-159	0.00901318993551451	7.689100725785946E-4	8.575302165368056E-4	0.0012563208643487547	7.949748208015979E-4	2.788928059861343E-4
160-164	0.009078351806072017	7.71516547400895E-4	8.809884899375085E-4	0.0012797791377494574	8.080071949130994E-4	2.8931870527533563E-4
165	0.00917479137449713	7.819424466900963E-4	8.862014395821091E-4	0.0012902050370386588	8.080071949130995E-4	2.997446045645369E-4
>>END_MODULE
