##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293778
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8405926
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.07495402648084	41.0	40.0	41.0	38.0	41.0
2	40.205252580144055	41.0	40.0	41.0	38.0	41.0
3	40.254818802830286	41.0	40.0	41.0	38.0	41.0
4	40.27719373213611	41.0	40.0	41.0	39.0	41.0
5	40.26996240509374	41.0	40.0	41.0	39.0	41.0
6	40.295285968494134	41.0	40.0	41.0	39.0	41.0
7	40.26135264574064	41.0	40.0	41.0	39.0	41.0
8	40.27010980110936	41.0	40.0	41.0	39.0	41.0
9	40.23897533716095	41.0	40.0	41.0	39.0	41.0
10-14	40.210633736247495	41.0	40.0	41.0	38.2	41.0
15-19	40.12022470814043	41.0	40.0	41.0	38.0	41.0
20-24	40.007148837617656	41.0	40.0	41.0	38.0	41.0
25-29	39.84656862313563	41.0	40.0	41.0	37.6	41.0
30-34	39.607395520731444	40.6	39.2	41.0	36.6	41.0
35-39	39.22953257023676	40.0	38.4	41.0	35.4	41.0
40-44	38.80095413640329	40.0	37.4	41.0	35.0	41.0
45-49	38.261429115602496	39.0	36.2	41.0	35.0	41.0
50-54	37.583715416957034	37.6	35.0	40.4	35.0	41.0
55-59	36.79433468721947	36.2	35.0	39.0	34.6	41.0
60-64	35.8719815520622	35.2	34.8	37.0	33.4	39.6
65-69	35.58732294336163	35.0	35.0	36.2	34.0	38.8
70-74	35.306077046121985	35.0	35.0	35.8	34.0	37.0
75-79	35.1395988020832	35.0	35.0	35.0	34.0	36.4
80-84	35.07643836027107	35.0	35.0	35.0	34.0	36.0
85-89	35.956167422839556	36.2	35.8	36.2	34.6	37.0
90-94	40.056224858536154	41.0	40.0	41.0	38.0	41.0
95-99	40.01396555763226	41.0	40.0	41.0	38.0	41.0
100-104	39.90076121686502	41.0	40.0	41.0	38.0	41.0
105-109	39.772450499843515	41.0	40.0	41.0	37.0	41.0
110-114	39.59997715781039	41.0	39.4	41.0	36.4	41.0
115-119	39.36842028447178	40.0	38.8	41.0	35.4	41.0
120-124	39.06892837907857	40.0	38.0	41.0	35.0	41.0
125-129	38.14085172648052	39.2	36.8	40.2	34.2	40.6
130-134	38.417186813642196	39.6	36.4	41.0	35.0	41.0
135-139	37.9292378391915	38.6	35.2	41.0	35.0	41.0
140-144	37.155442552559954	36.8	35.0	39.6	34.6	41.0
145-149	36.38248852343521	35.6	35.0	38.2	34.0	40.6
150-154	35.72258151577023	35.0	35.0	36.6	34.0	39.0
155-159	35.23996467156685	35.0	35.0	36.0	34.0	37.0
160-164	34.94390867472692	35.0	35.0	35.0	34.0	36.2
165-169	34.7888009820039	35.0	35.0	35.0	34.0	36.0
170-174	34.709795381870414	35.0	35.0	35.0	34.0	36.0
175-176	34.46073019491853	35.0	34.5	35.0	33.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	4.0
29	42.0
30	478.0
31	3116.0
32	14879.0
33	51324.0
34	136468.0
35	326077.0
36	880625.0
37	2713962.0
38	3437181.0
39	833664.0
40	8106.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.842792810690934	28.679184185061825	29.01134271227227	20.46668029197497
2	19.41699225046711	26.935735575116887	31.29454149370337	22.352730680712632
3	20.549645571469462	26.06538530079851	31.546911072022287	21.838058055709748
4	21.80894764003395	26.965595462058552	29.013281820468084	22.212175077439415
5	22.096780295234574	27.32429478917611	28.35009492113064	22.22882999445867
6	22.046030383803046	27.83645728025681	28.018638279708863	22.09887405623128
7	22.199136656687198	27.865805623318597	27.741738387894443	22.193319332099758
8	22.54287035122603	27.89300072353718	27.39263943080156	22.171489494435235
9	22.20691688220905	28.155006360988665	27.60727372570256	22.030803031099726
10-14	22.351055671915265	27.959568047589283	27.535024695673027	22.15435158482242
15-19	22.173473808834387	28.021034208485773	27.769511651660984	22.035980331018855
20-24	22.242106342596877	27.7901256803831	27.803925468770487	22.163842508249537
25-29	22.253184241688544	27.914723493878007	27.606702699976182	22.225389564457267
30-34	22.09403699247412	28.014971818690764	27.733058796853555	22.15793239198156
35-39	22.117363393396516	27.989998960257324	27.746268525323682	22.14636912102248
40-44	22.147337485483458	27.993087257727467	27.692330386919895	22.167244869869187
45-49	22.223931069581152	27.90416903503552	27.663731515123974	22.208168380259355
50-54	22.25273455892902	27.96339154068213	27.668066552096697	22.115807348292147
55-59	22.2233529060332	27.883654935815517	27.668085586287578	22.224906571863706
60-64	22.157187679263416	28.001224374328302	27.680619600981498	22.160968345426785
65-69	22.190982885169344	27.904073864081123	27.720053685935376	22.184889564814156
70-74	22.16596482053256	27.979727635004163	27.679891543180368	22.174416001282903
75-79	22.10969023519836	27.93791665546425	27.719739621785866	22.23265348755152
80-84	22.165051179370362	27.978488033323156	27.673122509048977	22.183338278257505
85-89	22.058528709389066	28.09454901220877	27.929489267452507	21.917433010949654
90-94	21.112837824493425	27.155910320994987	29.5686468507865	22.162605003725087
95-99	22.166778692514676	28.02261296082519	27.700043750908698	22.110564595751438
100-104	22.06595272145172	28.013821627800905	27.754178754835714	22.166046895911663
105-109	22.054235791722153	27.933191297746546	27.91945738864383	22.093115521887473
110-114	22.071287176904427	27.760398474129676	27.872548274055653	22.295766074910244
115-119	22.046330219796737	27.964037855871894	27.773268616995477	22.216363307335897
120-124	21.9759736873762	27.87124667335818	27.917501995582988	22.235277643682636
125-129	21.99258878963523	27.9597642084077	27.835721002009578	22.21192599994749
130-134	22.080571950134992	27.84229341990531	27.837261981628703	22.23987264833099
135-139	22.173857134905965	27.88611203013635	27.789470436249843	22.150560398707846
140-144	22.13276841858851	27.874599924929168	27.82691632427371	22.165715332208617
145-149	22.08330268287813	27.93146104898942	27.818435563676807	22.16680070445564
150-154	22.080838112646973	27.952522700177703	27.86656148427664	22.100077702898684
155-159	22.020437512240505	27.950991235731497	27.89843195273426	22.130139299293738
160-164	21.948293311838732	28.0663022685761	27.920558054316114	22.06484636526905
165-169	21.988017321161873	28.00803121669163	27.93241185067271	22.071539611473785
170-174	21.92242488860579	28.140084279188965	27.946510109048152	21.990980723157094
175-176	21.85773983302843	28.144464682661628	27.92294962869974	22.074845855610203
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	3.0
11	8.0
12	14.0
13	30.0
14	71.5
15	139.0
16	271.5
17	592.0
18	1192.5
19	2215.5
20	3869.5
21	6547.5
22	10648.5
23	16417.5
24	23844.5
25	33528.0
26	45128.5
27	58554.0
28	73850.0
29	89819.0
30	106470.5
31	123859.0
32	142242.5
33	160891.5
34	179975.5
35	200255.0
36	220542.0
37	242129.5
38	265256.0
39	288354.0
40	311556.5
41	333318.5
42	353375.5
43	370982.0
44	384122.5
45	391962.0
46	393294.0
47	387928.5
48	376928.5
49	360665.0
50	339241.5
51	313128.0
52	283182.5
53	251449.5
54	219286.5
55	188180.5
56	159008.0
57	132278.5
58	108430.0
59	87741.0
60	70527.0
61	56402.5
62	45461.0
63	36831.0
64	29689.0
65	24381.0
66	20264.5
67	16700.5
68	13882.0
69	11466.0
70	9263.0
71	7393.0
72	5799.5
73	4491.5
74	3407.5
75	2499.0
76	1744.5
77	1213.0
78	824.0
79	519.0
80	333.0
81	206.5
82	119.5
83	64.5
84	33.0
85	18.0
86	7.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	621.0
92-93	1181.0
94-95	2025.0
96-97	2563.0
98-99	3150.0
100-101	4146.0
102-103	4196.0
104-105	4818.0
106-107	4986.0
108-109	6015.0
110-111	6313.0
112-113	6140.0
114-115	6453.0
116-117	7317.0
118-119	7218.0
120-121	7509.0
122-123	7985.0
124-125	8408.0
126-127	8760.0
128-129	9101.0
130-131	9615.0
132-133	46468.0
134-135	68236.0
136-137	86345.0
138-139	76490.0
140-141	89607.0
142-143	60218.0
144-145	62685.0
146-147	64115.0
148-149	65345.0
150-151	66305.0
152-153	65960.0
154-155	62061.0
156-157	60011.0
158-159	62541.0
160-161	75737.0
162-163	65327.0
164-165	68467.0
166-167	64965.0
168-169	77241.0
170-171	69660.0
172-173	75639.0
174-175	128966.0
176-177	6725017.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.56001400972663
#Duplication Level	Percentage of total
1	79.40983356369642
2	15.287914828146661
3	3.362562003302097
4	0.9836049912700796
5	0.3434537704116577
6	0.1645233039048945
7	0.09826622175796541
8	0.057228365305190765
9	0.049193677893671614
>10	0.23863036782950778
>50	0.004788906481821873
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.4982375528882837E-4	1.1896369299468018E-5	0.0	1.1896369299468018E-5	0.0	0.0
2	2.4982375528882837E-4	2.3792738598936035E-5	2.3792738598936035E-5	1.1896369299468018E-5	0.0	0.0
3	2.4982375528882837E-4	4.758547719787207E-5	2.3792738598936035E-5	4.758547719787207E-5	0.0	0.0
4	2.4982375528882837E-4	7.137821579680811E-5	2.3792738598936035E-5	4.758547719787207E-5	0.0	0.0
5	2.617201245882964E-4	7.137821579680811E-5	3.5689107898404055E-5	4.758547719787207E-5	0.0	0.0
6	2.617201245882964E-4	7.137821579680811E-5	3.5689107898404055E-5	4.758547719787207E-5	0.0	0.0
7	2.617201245882964E-4	7.137821579680811E-5	3.5689107898404055E-5	7.137821579680811E-5	0.0	0.0
8	2.617201245882964E-4	7.137821579680811E-5	3.5689107898404055E-5	7.137821579680811E-5	1.1896369299468018E-5	0.0
9	2.8551286318723244E-4	8.327458509627613E-5	3.5689107898404055E-5	8.327458509627613E-5	1.1896369299468018E-5	0.0
10-14	3.0692632792627485E-4	1.0230877597542496E-4	3.5689107898404055E-5	1.0944659755510576E-4	1.1896369299468018E-5	0.0
15-19	3.3309834038510453E-4	1.1896369299468019E-4	5.9481846497340094E-5	1.5465280089308424E-4	2.1413464739042432E-5	0.0
20-24	3.830630914428702E-4	1.5703207475297785E-4	6.899894193691451E-5	1.9034190879148828E-4	3.5689107898404055E-5	0.0
25-29	4.306485686407423E-4	1.7606626563212665E-4	7.137821579680811E-5	2.783750416075516E-4	3.806838175829766E-5	0.0
30-34	6.209904774322307E-4	2.0223827809095633E-4	1.0468804983531855E-4	3.3071906652521095E-4	4.758547719787207E-5	2.3792738598936036E-6
35-39	0.0010968452494109515	2.355481121294668E-4	1.2610151457436098E-4	4.2113147320116786E-4	6.66196680770209E-5	1.903419087914883E-5
40-44	0.00158459639068914	2.498237552888283E-4	1.7368699177223304E-4	5.091646060172312E-4	1.0468804983531855E-4	2.617201245882964E-5
45-49	0.0019462460173929677	2.9265068476691324E-4	2.0699682581074353E-4	5.615086309348905E-4	1.332393361540418E-4	4.520620333797847E-5
50-54	0.0022912407270775396	3.2358124494553014E-4	2.1413464739042433E-4	6.471624898910603E-4	1.4275643159361622E-4	7.375748965670172E-5
55-59	0.002598167055003815	3.4737398354446614E-4	2.593408507284028E-4	6.804723239295707E-4	1.6892844405244585E-4	8.327458509627613E-5
60-64	0.0028051638808145587	3.7592526986318935E-4	2.9978850634659404E-4	7.280578011274426E-4	2.1413464739042433E-4	8.327458509627613E-5
65-69	0.0030407119929440254	3.9733873460223176E-4	3.378568881048917E-4	7.68505456745634E-4	2.617201245882964E-4	1.1658441913478658E-4
70-74	0.011656062639618764	4.0923510390169983E-4	3.5927035284393416E-4	7.994360169242508E-4	3.283397926653173E-4	1.4275643159361622E-4
75-79	0.06858256901143314	4.3778639022042304E-4	3.8068381758297657E-4	8.232287555231868E-4	3.521325312642534E-4	1.4275643159361622E-4
80-84	0.08037425026106583	4.7585477197872075E-4	3.9733873460223176E-4	8.660556850012716E-4	5.163024275969119E-4	1.4275643159361622E-4
85-89	0.0804932139540605	5.04406058297444E-4	4.116143777615934E-4	8.969862451798886E-4	7.708847306055276E-4	1.4275643159361622E-4
90-94	0.08060266055161561	5.567500832151034E-4	4.3540711636052946E-4	9.041240667595695E-4	7.732640044654212E-4	1.4275643159361622E-4
95-99	0.08071924497075039	5.876806433937202E-4	4.5206203337978476E-4	9.18399709918931E-4	7.732640044654212E-4	1.4275643159361622E-4
100-104	0.08082869156830551	5.948184649734009E-4	4.5206203337978476E-4	9.493302700975478E-4	7.780225521852084E-4	1.4275643159361622E-4
105-109	0.08098334436919859	5.948184649734009E-4	4.710962242589336E-4	9.85019377995952E-4	7.994360169242508E-4	1.4275643159361622E-4
110-114	0.08105948113271519	6.067148342728691E-4	4.901304151380823E-4	0.0010159499381745688	8.113323862237188E-4	1.4275643159361622E-4
115-119	0.08114513499167135	6.30507572871805E-4	5.091646060172313E-4	0.0010587768676526537	8.327458509627612E-4	1.451357054535098E-4
120-124	0.08172805708734529	6.54300311470741E-4	5.234402491765928E-4	0.0011135001664302068	8.731935065809526E-4	1.5465280089308424E-4
125-129	0.08249180399637114	6.661966807702091E-4	5.543708093552097E-4	0.0011729820129275466	8.874691497403142E-4	1.5941134861287144E-4
130-134	0.08316275922486113	6.78093050069677E-4	5.900599172536136E-4	0.0012015332992462698	9.18399709918931E-4	1.6654917019255227E-4
135-139	0.08393126468160676	7.351956227071236E-4	5.948184649734009E-4	0.0012633944196035036	9.469509962376542E-4	1.7844553949202026E-4
140-144	0.08459746136237697	7.518505397263787E-4	6.043355604129754E-4	0.0013942544818976517	9.826401041360584E-4	1.7844553949202026E-4
145-149	0.08517562491033112	7.780225521852084E-4	6.18611203572337E-4	0.0014632534238345663	0.0010064328427349944	1.8558336107170106E-4
150-154	0.08571809935038685	7.946774692044637E-4	6.733345023498899E-4	0.0015203559964720127	0.0010230877597542495	2.0223827809095633E-4
155-159	0.08627247015974207	8.041945646440381E-4	6.899894193691451E-4	0.0015536658305105235	0.0010302255813339303	2.2603101668989233E-4
160-164	0.08691487410191333	8.232287555231868E-4	6.97127240948826E-4	0.0015917342122688208	0.0010349841290537177	2.4982375528882837E-4
165	0.08742641798179046	8.565385895616973E-4	7.01885788668613E-4	0.0016179062247276505	0.0010349841290537177	2.4982375528882837E-4
>>END_MODULE
