##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293779
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8423856
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.05989074362145	41.0	40.0	41.0	38.0	41.0
2	40.197248267301816	41.0	40.0	41.0	38.0	41.0
3	40.243515558670516	41.0	40.0	41.0	38.0	41.0
4	40.265088933144156	41.0	40.0	41.0	39.0	41.0
5	40.27431546788074	41.0	40.0	41.0	39.0	41.0
6	40.301191639553196	41.0	40.0	41.0	39.0	41.0
7	40.25915340908012	41.0	40.0	41.0	39.0	41.0
8	40.27195099251459	41.0	40.0	41.0	39.0	41.0
9	40.23851072477972	41.0	40.0	41.0	39.0	41.0
10-14	40.20656986539181	41.0	40.0	41.0	38.2	41.0
15-19	40.119329983798394	41.0	40.0	41.0	38.0	41.0
20-24	40.00675139745979	41.0	40.0	41.0	38.0	41.0
25-29	39.84658434332211	41.0	40.0	41.0	37.6	41.0
30-34	39.60344177298377	40.6	39.2	41.0	36.6	41.0
35-39	39.228493245848455	40.0	38.4	41.0	35.4	41.0
40-44	38.79435823689294	40.0	37.4	41.0	35.0	41.0
45-49	38.251499028473425	39.0	36.2	41.0	35.0	41.0
50-54	37.58015540626526	37.6	35.0	40.4	35.0	41.0
55-59	36.7873268963762	36.2	35.0	39.0	34.6	41.0
60-64	35.8732680853044	35.2	34.8	37.0	33.4	39.6
65-69	35.592638952992544	35.0	35.0	36.2	34.0	38.8
70-74	35.30698250302474	35.0	35.0	35.8	34.0	37.0
75-79	35.139165769215424	35.0	35.0	35.0	34.0	36.4
80-84	35.07792780408403	35.0	35.0	35.0	34.0	36.0
85-89	35.97094233329725	36.2	35.8	36.2	34.6	37.0
90-94	40.07726082103872	41.0	40.0	41.0	38.0	41.0
95-99	40.03136710736051	41.0	40.0	41.0	38.0	41.0
100-104	39.924118948935714	41.0	40.0	41.0	38.0	41.0
105-109	39.79030500850029	41.0	40.0	41.0	37.2	41.0
110-114	39.62211355754868	41.0	39.4	41.0	36.6	41.0
115-119	39.39326683410308	40.2	39.0	41.0	35.6	41.0
120-124	39.09115157963795	40.0	38.0	41.0	35.0	41.0
125-129	38.15982659867525	39.2	37.0	40.2	34.2	40.6
130-134	38.442639238710356	39.6	36.4	41.0	35.0	41.0
135-139	37.9404963915873	38.6	35.2	41.0	35.0	41.0
140-144	37.16563621510196	36.8	35.0	39.6	34.8	41.0
145-149	36.38967801445297	35.6	35.0	38.2	34.0	40.6
150-154	35.72598185049544	35.0	35.0	36.6	34.0	39.0
155-159	35.24514443402949	35.0	35.0	36.0	34.0	37.0
160-164	34.95135974942048	35.0	35.0	35.0	34.0	36.2
165-169	34.792816598288894	35.0	35.0	35.0	34.0	36.0
170-174	34.70750783352844	35.0	35.0	35.0	34.0	36.0
175-176	34.45797223398795	35.0	34.5	35.0	33.0	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	23.0
30	396.0
31	2932.0
32	14170.0
33	50305.0
34	135905.0
35	322277.0
36	875242.0
37	2718223.0
38	3457387.0
39	838711.0
40	8283.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.844556697075543	28.65926245652822	29.017578173226134	20.4786026731701
2	19.42463166511868	26.92351341238502	31.287548125229108	22.36430679726719
3	20.573072474173348	26.05234467445787	31.54075758180102	21.83382526956776
4	21.80725786385712	26.93806731739004	29.017423849600466	22.23725096915237
5	22.13316561916538	27.32289108455795	28.287200066097995	22.256743230178675
6	22.06812414647164	27.82444286796925	28.0112931655052	22.096139820053903
7	22.23880607645715	27.839875230535753	27.71697426926576	22.204344423741336
8	22.54899656404383	27.85349132273866	27.40245084911233	22.195061264105178
9	22.229071816992125	28.154849750518053	27.57967372661641	22.036404705873412
10-14	22.35327147092733	27.964960464661313	27.51944952525304	22.162318539158317
15-19	22.187632362186626	28.000103515539678	27.766789935630428	22.045474186643265
20-24	22.248469109633405	27.787400449390397	27.797813732808347	22.16631670816785
25-29	22.270138520886395	27.90669023781983	27.57554735028709	22.247623891006686
30-34	22.116510538641688	27.982256581783925	27.73116254598844	22.17007033358595
35-39	22.120252293011657	27.969466714530732	27.74269170793043	22.167589284527182
40-44	22.15968791489313	27.9791772318995	27.68619501567928	22.174939837528086
45-49	22.233283664867965	27.904904832181366	27.644912258709077	22.216899244241592
50-54	22.275057883230673	27.92079066878636	27.670601206858237	22.13355024112473
55-59	22.238378718724537	27.862119200518148	27.653962745801923	22.245539334955396
60-64	22.173083205600854	27.971821930479347	27.683122788423738	22.17197207549607
65-69	22.211101424335837	27.867332964856	27.722135800991847	22.199429809816312
70-74	22.16864580781058	27.955413767756713	27.692683730586086	22.183256693846616
75-79	22.11537566644064	27.917504762664507	27.733731440803354	22.23338813009149
80-84	22.18392859517067	27.955667808186657	27.670755530483902	22.189648066158778
85-89	22.067995939151857	28.06964886389321	27.934905345010648	21.92744985194429
90-94	21.133039991628713	27.12733818035018	29.558352173833658	22.181269654187453
95-99	22.187009999499864	28.005995922174186	27.675775901814752	22.131218176511197
100-104	22.07138732868922	28.005308944270546	27.74542973840306	22.177873988637174
105-109	22.082618832039216	27.901837199205264	27.91260637899895	22.102937589756564
110-114	22.082031625621664	27.757628367091236	27.850356325708926	22.309983681578174
115-119	22.044904978243643	27.93586033238978	27.77314848840685	22.24608620095973
120-124	21.997845747498328	27.873768212523714	27.897416538410823	22.23096950156714
125-129	22.02157066623871	27.928359806765883	27.83152172346226	22.21854780353314
130-134	22.09317183210232	27.832529509257025	27.83166625831039	22.242632400330265
135-139	22.2034319448281	27.87797998963605	27.770213546809313	22.148374518726538
140-144	22.16781231065988	27.86018260288335	27.803084489350233	22.16892059710654
145-149	22.11754851337928	27.914722112598422	27.77843924533513	22.189290128687166
150-154	22.09052948796607	27.95118961698837	27.852664696762364	22.105616198283197
155-159	22.05142203528039	27.929927058692734	27.875006935237217	22.143643970789665
160-164	21.976289283367645	28.0517870754747	27.903307146129464	22.06861649502819
165-169	22.011259799322	27.98754355273661	27.907551486722397	22.093645161218987
170-174	21.959094870404815	28.111784054717276	27.909440004452897	22.01968107042501
175-176	21.891841529611924	28.11381497057589	27.89441936172877	22.09992413808342
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	0.5
10	3.0
11	8.0
12	15.5
13	31.0
14	65.5
15	148.5
16	296.0
17	595.0
18	1175.0
19	2156.5
20	3901.0
21	6575.5
22	10459.5
23	16220.5
24	23865.0
25	33506.0
26	45338.0
27	58880.5
28	73480.5
29	89295.0
30	106483.5
31	124407.0
32	142453.5
33	160772.5
34	179887.5
35	199846.0
36	220622.0
37	242404.5
38	264899.0
39	288479.5
40	312155.5
41	333719.5
42	353915.5
43	371249.5
44	383159.0
45	391138.5
46	394186.5
47	389396.0
48	377455.5
49	361147.0
50	339753.0
51	313394.0
52	284175.5
53	252820.5
54	220704.0
55	189555.0
56	160439.0
57	133370.5
58	109309.5
59	88590.5
60	71320.5
61	56990.0
62	45635.5
63	37236.0
64	30409.5
65	24786.0
66	20291.5
67	16870.5
68	14100.5
69	11619.0
70	9462.5
71	7621.0
72	6091.5
73	4662.5
74	3504.0
75	2608.5
76	1817.5
77	1235.5
78	840.0
79	536.5
80	340.0
81	223.0
82	122.5
83	66.0
84	41.0
85	13.5
86	5.0
87	5.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	552.0
92-93	1521.0
94-95	2439.0
96-97	3121.0
98-99	3288.0
100-101	3752.0
102-103	5382.0
104-105	5011.0
106-107	4860.0
108-109	6048.0
110-111	5873.0
112-113	6336.0
114-115	7206.0
116-117	6604.0
118-119	7074.0
120-121	8360.0
122-123	8538.0
124-125	8543.0
126-127	9046.0
128-129	9105.0
130-131	10283.0
132-133	43084.0
134-135	74554.0
136-137	83603.0
138-139	69254.0
140-141	70301.0
142-143	73426.0
144-145	69730.0
146-147	65048.0
148-149	56847.0
150-151	66526.0
152-153	58308.0
154-155	61350.0
156-157	64983.0
158-159	64700.0
160-161	59330.0
162-163	65003.0
164-165	75537.0
166-167	70747.0
168-169	71861.0
170-171	78107.0
172-173	84911.0
174-175	131837.0
176-177	6741867.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.58194395873889
#Duplication Level	Percentage of total
1	79.49431917526387
2	15.126977079482462
3	3.37962020383679
4	0.9896542689278247
5	0.3792065661674469
6	0.17890537588052738
7	0.11480588073711681
8	0.062424378921081686
9	0.043955062822525134
>10	0.21983890676528242
>50	0.010293101195018018
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.3742096256156327E-4	1.1871048128078163E-5	0.0	0.0	1.1871048128078163E-5	0.0
2	2.3742096256156327E-4	3.561314438423449E-5	1.1871048128078163E-5	2.3742096256156326E-5	1.1871048128078163E-5	0.0
3	2.492920106896414E-4	4.748419251231265E-5	1.1871048128078163E-5	3.561314438423449E-5	2.3742096256156326E-5	0.0
4	2.492920106896414E-4	4.748419251231265E-5	1.1871048128078163E-5	5.9355240640390816E-5	2.3742096256156326E-5	0.0
5	2.492920106896414E-4	4.748419251231265E-5	2.3742096256156326E-5	7.122628876846897E-5	2.3742096256156326E-5	0.0
6	2.611630588177196E-4	4.748419251231265E-5	2.3742096256156326E-5	8.309733689654714E-5	2.3742096256156326E-5	0.0
7	2.611630588177196E-4	4.748419251231265E-5	2.3742096256156326E-5	1.1871048128078163E-4	2.3742096256156326E-5	0.0
8	2.967762032019541E-4	4.748419251231265E-5	2.3742096256156326E-5	1.1871048128078163E-4	2.3742096256156326E-5	0.0
9	3.0864725133003225E-4	7.122628876846897E-5	3.561314438423449E-5	1.1871048128078163E-4	2.3742096256156326E-5	0.0
10-14	3.395119764630355E-4	8.309733689654714E-5	4.7484192512312645E-5	1.2583311015762852E-4	2.3742096256156326E-5	0.0
15-19	3.632540727191918E-4	9.49683850246253E-5	5.9355240640390816E-5	2.1842728555663816E-4	3.561314438423449E-5	0.0
20-24	4.20235103733967E-4	1.377041582857067E-4	6.64778695172377E-5	2.896535743251072E-4	5.4606821389159555E-5	0.0
25-29	4.4160299036450767E-4	2.0418202780294437E-4	7.834891764531587E-5	3.347635572118042E-4	5.9355240640390816E-5	0.0
30-34	5.864297775270613E-4	2.397951721871789E-4	1.0446522352708784E-4	3.656282823448074E-4	7.122628876846897E-5	2.3742096256156326E-6
35-39	0.0010802653796551129	2.9202778395072285E-4	1.1871048128078163E-4	4.131124748571201E-4	8.309733689654714E-5	1.1871048128078163E-5
40-44	0.001621585174295477	3.133956705812635E-4	1.4245257753693798E-4	4.5584824811820146E-4	1.1396206202955038E-4	4.510998288669702E-5
45-49	0.0019492261026304344	3.395119764630355E-4	1.7331730266994115E-4	5.10455069507361E-4	1.543236256650161E-4	8.072312727093151E-5
50-54	0.002165279178561457	3.632540727191918E-4	1.8993677004925058E-4	6.244171315369113E-4	1.9231097967486625E-4	1.1158785240393474E-4
55-59	0.002397951721871789	3.8224774972411683E-4	2.2554991443348506E-4	7.217597261871522E-4	2.0180781817732879E-4	1.305815294088598E-4
60-64	0.0025878884919210395	4.2498352298519815E-4	2.492920106896414E-4	8.214765304630089E-4	2.0180781817732879E-4	1.305815294088598E-4
65-69	0.0028253094544826033	4.5347403849258584E-4	2.967762032019541E-4	8.760833518521683E-4	2.4216938181279452E-4	1.305815294088598E-4
70-74	0.011220514690659478	4.819645539999733E-4	3.632540727191918E-4	8.784575614777839E-4	2.682856876945665E-4	1.5907204491624738E-4
75-79	0.06608375071938552	5.294487465122861E-4	4.0361563635465757E-4	9.093222866107872E-4	3.395119764630355E-4	1.6619467379309428E-4
80-84	0.07780284943142428	5.888039871526768E-4	4.3685457111327633E-4	9.710517368767936E-4	5.508166331428268E-4	1.6619467379309428E-4
85-89	0.0778835725586952	6.125460834088333E-4	4.5584824811820146E-4	0.0010019164620097966	7.146370973103054E-4	1.6619467379309428E-4
90-94	0.07799990883035038	6.719013240492241E-4	4.65345086620664E-4	0.0010707685411526502	7.312565646896147E-4	1.6619467379309428E-4
95-99	0.07807350932874446	7.24133935812768E-4	5.10455069507361E-4	0.0011324979914186567	7.360049839408461E-4	1.6619467379309428E-4
100-104	0.07810199984425184	7.716181283250806E-4	5.365713753891329E-4	0.0011966016513102788	7.360049839408461E-4	1.6619467379309428E-4
105-109	0.07819696822927646	8.119796919605465E-4	5.626876812709049E-4	0.0012464600534482072	7.360049839408461E-4	1.6619467379309428E-4
110-114	0.0783204271298085	8.333475785910871E-4	5.959266160295237E-4	0.0013366800192216011	7.43127612817693E-4	1.6619467379309428E-4
115-119	0.07846287970734542	8.737091422265527E-4	6.054234545319863E-4	0.0013936610502363763	7.929860149556211E-4	1.6619467379309428E-4
120-124	0.07887361797257693	9.23567544364481E-4	6.054234545319863E-4	0.0014910036448866175	8.262249497142402E-4	1.7094309304432553E-4
125-129	0.07946004775010398	9.473096406206374E-4	6.054234545319863E-4	0.0015337394181476984	8.452186267191651E-4	1.8993677004925058E-4
130-134	0.08014856854153253	9.710517368767936E-4	6.26791341162527E-4	0.0015930946587880894	8.737091422265529E-4	2.0180781817732879E-4
135-139	0.08083234091370983	9.829227850048719E-4	6.719013240492241E-4	0.0016405788513004019	9.235675443644809E-4	2.1842728555663816E-4
140-144	0.08146862909337481	9.971680427585657E-4	7.265081454383836E-4	0.001737921445950643	9.615548983743312E-4	2.2792412405910073E-4
145-149	0.08192922576074425	0.001013787510137875	7.407534031920774E-4	0.0017949024769654182	9.852969946304874E-4	2.611630588177196E-4
150-154	0.08240644189549298	0.0010446522352708784	7.716181283250806E-4	0.0018186445732215745	9.852969946304874E-4	2.611630588177196E-4
155-159	0.08283617383772943	0.0010755169604038817	7.787407572019273E-4	0.001830515621349653	9.924196235073343E-4	2.6828568769456644E-4
160-164	0.08342022940563086	0.0010826395892807286	8.024828534580837E-4	0.0018590061368570405	0.0010185359293891064	2.730341069457977E-4
165	0.08389269712112837	0.0010921364277831909	8.07231272709315E-4	0.0018637545561082716	0.0010327811871428001	2.730341069457977E-4
>>END_MODULE
