##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293781
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8515431
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-176
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.1472223778221	41.0	40.0	41.0	38.0	41.0
2	40.229252753031524	41.0	40.0	41.0	38.0	41.0
3	40.259434666313425	41.0	40.0	41.0	39.0	41.0
4	40.264012238487986	41.0	40.0	41.0	38.0	41.0
5	40.26478800661998	41.0	40.0	41.0	39.0	41.0
6	40.28921131531686	41.0	40.0	41.0	39.0	41.0
7	40.25268339324222	41.0	40.0	41.0	39.0	41.0
8	40.25853336137654	41.0	40.0	41.0	39.0	41.0
9	40.22101864250911	41.0	40.0	41.0	39.0	41.0
10-14	40.19027654619009	41.0	40.0	41.0	38.2	41.0
15-19	40.10508536796317	41.0	40.0	41.0	38.0	41.0
20-24	39.997947608288996	41.0	40.0	41.0	38.0	41.0
25-29	39.831431832399325	41.0	40.0	41.0	37.6	41.0
30-34	39.59447530019326	40.4	39.2	41.0	36.6	41.0
35-39	39.21789978686927	40.0	38.4	41.0	35.4	41.0
40-44	38.78645912344307	40.0	37.4	41.0	35.0	41.0
45-49	38.253914405506904	39.0	36.2	41.0	35.0	41.0
50-54	37.57684652720455	37.6	35.0	40.4	35.0	41.0
55-59	36.76674707363609	36.2	35.0	39.0	34.4	41.0
60-64	35.84318106740575	35.2	34.8	37.0	33.4	39.4
65-69	35.54253662556833	35.0	35.0	36.2	34.0	38.4
70-74	35.25054790532622	35.0	35.0	35.8	34.0	37.0
75-79	35.079360985955965	35.0	35.0	35.0	34.0	36.2
80-84	35.013463064875985	35.0	35.0	35.0	34.0	36.0
85-89	35.915361559502976	36.2	35.8	36.2	34.6	37.0
90-94	40.065201133120034	41.0	40.0	41.0	38.0	41.0
95-99	40.020604710876384	41.0	40.0	41.0	38.0	41.0
100-104	39.91020211598477	41.0	40.0	41.0	38.0	41.0
105-109	39.78086463799887	41.0	40.0	41.0	37.2	41.0
110-114	39.61211675467125	41.0	39.4	41.0	36.6	41.0
115-119	39.38471643472285	40.0	39.0	41.0	35.6	41.0
120-124	39.08858237539836	40.0	38.0	41.0	35.0	41.0
125-129	38.1584390137965	39.2	37.0	40.2	34.2	40.6
130-134	38.45894788890824	39.6	36.4	41.0	35.0	41.0
135-139	37.95791703474411	38.8	35.4	41.0	35.0	41.0
140-144	37.17442845395348	36.8	35.0	39.6	34.6	41.0
145-149	36.38850630983112	35.6	35.0	38.2	34.0	40.6
150-154	35.72011798708381	35.0	35.0	36.6	34.0	39.0
155-159	35.231847105303494	35.0	35.0	36.0	34.0	37.0
160-164	34.93436883961145	35.0	35.0	35.0	34.0	36.2
165-169	34.77233334998299	35.0	35.0	35.0	34.0	36.0
170-174	34.68518926413448	35.0	35.0	35.0	34.0	36.0
175-176	34.423417577041874	35.0	34.5	35.0	32.5	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	50.0
30	477.0
31	3150.0
32	15358.0
33	53368.0
34	143728.0
35	342174.0
36	908730.0
37	2759416.0
38	3459042.0
39	823174.0
40	6761.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.874723663429368	28.690432698004365	29.039857172232388	20.394986466333883
2	19.343918117591464	26.996390435199345	31.314257610683477	22.345433836525714
3	20.51267868884147	26.08532674388413	31.58799595698679	21.813998610287605
4	21.772767579233513	26.963931714084698	29.032987290954505	22.230313415727284
5	22.09377305740602	27.378085736353214	28.322805974236655	22.20533523200411
6	22.02240849582364	27.88673879219971	28.0335898441312	22.057262867845445
7	22.178654257194967	27.882687323753785	27.774612935035233	22.164045484016018
8	22.495631753695143	27.904177721597417	27.445328369168863	22.154862155538574
9	22.186581043284832	28.198737092696774	27.604979712712137	22.009702151306257
10-14	22.336144817567074	27.991529729969038	27.551286599586096	22.121038852877795
15-19	22.152614471305093	28.036290823095158	27.791633799862858	22.01946090573689
20-24	22.20943837135196	27.815115876107736	27.83084027103267	22.14460548150763
25-29	22.22355157360796	27.941857552483253	27.634847842698747	22.199743031210048
30-34	22.07267723735886	28.03529733257189	27.748133946479044	22.143891483590203
35-39	22.069407878473797	28.019413227586483	27.783126890465088	22.128052003474632
40-44	22.119199838504944	28.030351018051817	27.70812422765213	22.142324915791107
45-49	22.205647606092985	27.932263205467816	27.673081961441532	22.18900722699767
50-54	22.254983922716303	27.985376195285944	27.659842467163436	22.099797414834317
55-59	22.213200952482616	27.914483717852917	27.672445469876976	22.199869859787487
60-64	22.139785995564992	28.01390323050002	27.700587322004015	22.14572345193097
65-69	22.178712974128967	27.90854626148694	27.742842376387056	22.169898387997037
70-74	22.14579860960649	28.000412427744408	27.70009879711315	22.153690165535956
75-79	22.08840163228379	27.956496858467883	27.74047725828558	22.214624250962753
80-84	22.152034347997184	28.00064729548041	27.684390842929734	22.162927513592674
85-89	22.026821660583003	28.11778992748576	27.946904860129806	21.90848355180143
90-94	21.081514222791796	27.147868930086776	29.620666830773395	22.149950016348036
95-99	22.13671872053318	28.05712468424498	27.70821525065011	22.09794134457173
100-104	22.038766004553334	28.045037800504936	27.792716113569547	22.12348008137218
105-109	22.0363736150563	27.968052759845342	27.921857844338234	22.073715780760132
110-114	22.046010913491717	27.790941509855916	27.888885400505785	22.27416217614658
115-119	22.014046176109193	28.003166996987556	27.782626170621015	22.200160656282236
120-124	21.936088339915162	27.923925602090904	27.934487727899178	22.205498330094755
125-129	21.988246224934382	27.981744454900976	27.84600140620528	22.184007913959366
130-134	22.052539752226057	27.88350319847195	27.844397023670563	22.219560025631424
135-139	22.1597783697705	27.918085505115197	27.805701087839253	22.116435037275046
140-144	22.11053710203769	27.908426082366628	27.82676473503145	22.15427208056423
145-149	22.07409090700857	27.95687708709253	27.83402957411973	22.135002431779167
150-154	22.049236013558975	27.998397078500265	27.889745837664442	22.06262107027632
155-159	22.00491063099291	27.983965064020584	27.899140927906487	22.111983377080023
160-164	21.91854985960111	28.09866985653769	27.936531578283454	22.04624870557775
165-169	21.95355793002426	28.03646106303156	27.950215312005728	22.059765694938452
170-174	21.901872437618955	28.173561648119843	27.960988066332405	21.9635778479288
175-176	21.837672639797294	28.17778531252853	27.945123587960342	22.039418459713836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	2.0
11	3.5
12	8.0
13	23.0
14	61.5
15	134.5
16	279.5
17	578.0
18	1191.5
19	2236.5
20	3983.5
21	6798.5
22	10875.5
23	16603.0
24	24406.5
25	34251.5
26	46194.0
27	60336.5
28	75396.0
29	91598.5
30	109203.5
31	126867.5
32	145049.5
33	163829.5
34	183147.5
35	203332.0
36	224436.5
37	246657.0
38	269366.0
39	292632.0
40	316523.0
41	339333.5
42	359628.0
43	375891.0
44	387960.0
45	396585.0
46	398298.5
47	393006.5
48	381907.0
49	364498.0
50	341674.0
51	314842.0
52	285928.0
53	254243.0
54	220857.5
55	189441.0
56	160496.5
57	133453.5
58	109270.0
59	88511.5
60	71130.0
61	56835.5
62	45399.5
63	36514.0
64	29864.0
65	24535.0
66	20089.0
67	16670.0
68	13766.0
69	11233.0
70	9274.5
71	7481.5
72	5871.0
73	4511.5
74	3384.0
75	2504.5
76	1756.5
77	1161.0
78	760.0
79	516.0
80	332.0
81	193.0
82	115.0
83	65.0
84	34.5
85	16.5
86	5.5
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-176	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	495.0
92-93	1110.0
94-95	1942.0
96-97	2372.0
98-99	2952.0
100-101	3526.0
102-103	3901.0
104-105	4373.0
106-107	4850.0
108-109	5287.0
110-111	5618.0
112-113	5884.0
114-115	6415.0
116-117	6821.0
118-119	6994.0
120-121	7485.0
122-123	8034.0
124-125	10685.0
126-127	8648.0
128-129	8957.0
130-131	9387.0
132-133	37261.0
134-135	58766.0
136-137	74401.0
138-139	66470.0
140-141	65420.0
142-143	60746.0
144-145	60255.0
146-147	61059.0
148-149	60692.0
150-151	63855.0
152-153	57791.0
154-155	72008.0
156-157	61910.0
158-159	62886.0
160-161	61276.0
162-163	64605.0
164-165	67788.0
166-167	66824.0
168-169	73898.0
170-171	70362.0
172-173	74353.0
174-175	133101.0
176-177	6923968.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.38237335073352
#Duplication Level	Percentage of total
1	79.12716115848242
2	15.418415818118703
3	3.408536994236263
4	1.0311362598677796
5	0.3750306653193482
6	0.18913162584263324
7	0.10339198364883616
8	0.09567757252755568
9	0.0334736191943407
>10	0.2151666736765243
>50	0.0028776290855980465
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.348677359959819E-4	1.1743386799799094E-5	1.1743386799799094E-5	0.0	0.0	0.0
2	2.348677359959819E-4	1.1743386799799094E-5	1.1743386799799094E-5	2.3486773599598187E-5	0.0	0.0
3	2.348677359959819E-4	4.6973547199196374E-5	1.1743386799799094E-5	4.6973547199196374E-5	0.0	0.0
4	2.4661112279578096E-4	4.6973547199196374E-5	2.3486773599598187E-5	5.871693399899547E-5	0.0	0.0
5	2.4661112279578096E-4	4.6973547199196374E-5	2.3486773599598187E-5	8.220370759859366E-5	0.0	0.0
6	2.4661112279578096E-4	4.6973547199196374E-5	3.523016039939728E-5	8.220370759859366E-5	1.1743386799799094E-5	0.0
7	2.5835450959558006E-4	4.6973547199196374E-5	3.523016039939728E-5	8.220370759859366E-5	1.1743386799799094E-5	0.0
8	2.5835450959558006E-4	5.871693399899547E-5	3.523016039939728E-5	9.394709439839275E-5	3.523016039939728E-5	0.0
9	2.5835450959558006E-4	7.046032079879456E-5	5.871693399899547E-5	1.0569048119819185E-4	4.6973547199196374E-5	0.0
10-14	2.5835450959558006E-4	8.92497396784731E-5	5.871693399899547E-5	1.4092064159758913E-4	4.6973547199196374E-5	0.0
15-19	2.5835450959558006E-4	1.1978254535795075E-4	7.515767551871421E-5	1.8319683407686584E-4	4.9322224559156195E-5	0.0
20-24	2.747952511152988E-4	1.3387460951770966E-4	1.1743386799799095E-4	2.0433493031650425E-4	5.871693399899547E-5	0.0
25-29	3.2881483039437465E-4	2.1138096239638372E-4	1.5501270575734804E-4	2.536571548756604E-4	5.871693399899547E-5	0.0
30-34	4.5564340783220484E-4	2.3956509071590155E-4	1.8789418879678552E-4	3.1237408887465587E-4	1.0099312647827222E-4	0.0
35-39	9.018921062245704E-4	2.7009789639537916E-4	2.0668360767646404E-4	3.6169631343381205E-4	1.4326931895754894E-4	9.394709439839274E-6
40-44	0.001376324932936454	2.8184128319517826E-4	2.137296397563435E-4	4.0632118327304866E-4	1.5971006047726767E-4	3.288148303943747E-5
45-49	0.0017497646331700652	3.2881483039437465E-4	2.348677359959819E-4	4.6268943991208437E-4	2.1138096239638372E-4	4.93222245591562E-5
50-54	0.0020198625295654443	3.945777964732496E-4	2.442624454358212E-4	5.026169550314013E-4	2.3251905863602208E-4	8.220370759859366E-5
55-59	0.002242986878761627	4.157158927128879E-4	2.8184128319517826E-4	5.354984380708386E-4	2.7244657375533895E-4	1.127365132780713E-4
60-64	0.002374512810919377	4.3215663423260665E-4	3.170714435945755E-4	6.036100815096734E-4	3.100254115146961E-4	1.3857196423762932E-4
65-69	0.0025999858374755195	4.6503811727204416E-4	3.49952926634013E-4	6.4823495134891E-4	3.3820953983421397E-4	1.6440741519718733E-4
70-74	0.011076362429570505	4.932222455915619E-4	3.6169631343381205E-4	6.717217249485082E-4	3.523016039939728E-4	1.6675609255714714E-4
75-79	0.06739294816668703	5.542878569505173E-4	4.0632118327304866E-4	6.905111438281868E-4	3.687423455136915E-4	1.8319683407686584E-4
80-84	0.07925376883448412	6.106561135895528E-4	4.2276192479276744E-4	7.492280778271821E-4	5.401957927907583E-4	1.9494022087666497E-4
85-89	0.07938999212136179	6.646756928686289E-4	4.603407625521245E-4	7.938529476664187E-4	7.280899815875438E-4	1.9963757559658457E-4
90-94	0.07952621540823947	7.163465947877447E-4	5.237550512710396E-4	8.619645911052536E-4	7.398333683873429E-4	1.9963757559658457E-4
95-99	0.07958962969695839	7.327873363074634E-4	5.777746305501155E-4	9.159841703843295E-4	7.398333683873429E-4	1.9963757559658457E-4
100-104	0.07968827414607668	7.703661740668206E-4	6.200508230293922E-4	9.629577175835257E-4	7.398333683873429E-4	1.9963757559658457E-4
105-109	0.07977282653103523	8.05596334466218E-4	6.364915645491109E-4	0.0010686481987817176	7.398333683873429E-4	2.0198625295654444E-4
110-114	0.079845635529194	8.220370759859366E-4	6.646756928686289E-4	0.0011461545516603913	7.398333683873429E-4	2.1138096239638372E-4
115-119	0.0799748127839918	8.220370759859366E-4	6.811164343883475E-4	0.0011978254535795075	7.398333683873429E-4	2.1138096239638372E-4
120-124	0.08044689693334371	8.220370759859366E-4	6.905111438281868E-4	0.001256542387578503	7.421820457473027E-4	2.1842699447626314E-4
125-129	0.08109982923941253	8.314317854257758E-4	7.163465947877447E-4	0.0012847265158980208	7.633201419869411E-4	2.3017038127606226E-4
130-134	0.08168230122468258	8.455238495855349E-4	7.421820457473026E-4	0.0013176079989374583	8.126423665460973E-4	2.489598001557408E-4
135-139	0.08231409543451176	8.666619458251731E-4	7.844582382265794E-4	0.001413903770695811	8.337804627857357E-4	2.8888731527505773E-4
140-144	0.08301634996513976	8.784053326249723E-4	8.03247657106258E-4	0.0015477783802135205	8.455238495855349E-4	2.959333473549372E-4
145-149	0.08348843411449168	9.089381383044499E-4	8.126423665460973E-4	0.0015900545726927975	8.619645911052534E-4	3.147227662346157E-4
150-154	0.08398400503744322	9.277275571841284E-4	8.455238495855349E-4	0.0016370281198919937	8.854513647048517E-4	3.2646615303441487E-4
155-159	0.08443729976791545	9.512143307837267E-4	8.619645911052534E-4	0.0016558175387716722	9.112868156644097E-4	3.569989587138924E-4
160-164	0.08488589714366776	9.676550723034452E-4	8.995434288646105E-4	0.0016886990218111097	9.159841703843295E-4	3.75788377593571E-4
165	0.08542139558173861	9.864444911831239E-4	9.159841703843294E-4	0.0017027910859708686	9.159841703843294E-4	3.992751511931692E-4
>>END_MODULE
