##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293792
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5294840
Total Bases	856.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-174
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.11700315779136	40.0	39.0	41.0	36.0	41.0
2	39.33870749635494	40.0	39.0	41.0	36.0	41.0
3	39.383785345732825	40.0	39.0	41.0	37.0	41.0
4	39.40913096524163	40.0	39.0	41.0	37.0	41.0
5	39.39704882489367	40.0	39.0	41.0	37.0	41.0
6	39.37558774202809	40.0	39.0	41.0	37.0	41.0
7	39.3623015992929	40.0	39.0	41.0	36.0	41.0
8	39.33475270263124	40.0	39.0	41.0	36.0	41.0
9	39.31744283113371	40.0	39.0	41.0	36.0	41.0
10-14	39.23630712165051	40.0	38.6	41.0	36.0	41.0
15-19	39.069611886289294	40.0	38.0	41.0	35.8	41.0
20-24	38.85336259452599	40.0	38.0	41.0	35.0	41.0
25-29	38.73974231515967	40.0	38.0	41.0	35.0	41.0
30-34	38.36850669708622	40.0	37.0	40.4	34.2	41.0
35-39	38.093544771891125	39.4	36.2	40.0	34.0	41.0
40-44	37.47726390221423	38.4	35.0	40.0	33.2	41.0
45-49	36.709736838129196	36.8	35.0	39.6	33.0	41.0
50-54	35.98661088909202	35.6	34.2	38.6	32.8	40.0
55-59	35.23984660537429	35.0	33.8	36.8	31.8	39.2
60-64	34.86436727077683	35.0	34.0	36.0	32.0	38.6
65-69	34.66238813637428	35.0	34.0	35.6	32.0	37.2
70-74	34.514093230390344	35.0	34.0	35.0	32.0	37.0
75-79	34.46718371093366	35.0	33.6	35.0	32.0	37.0
80-84	34.34028371017821	34.8	33.0	35.0	31.6	37.6
85-89	37.625662456278185	39.6	36.2	40.2	32.0	40.2
90-94	38.18541408421484	40.0	37.0	41.0	32.4	41.0
95-99	38.0993881641275	40.0	36.8	41.0	32.0	41.0
100-104	37.9608636877496	40.0	36.0	41.0	32.0	41.0
105-109	37.780289417357075	40.0	36.0	40.8	32.0	41.0
110-114	37.558209492734576	39.2	35.0	40.0	32.0	41.0
115-119	37.30386289192039	39.0	35.0	40.0	31.4	41.0
120-124	36.99463550916923	38.4	34.8	39.8	31.0	40.8
125-129	36.68618730752202	38.0	34.0	40.0	31.0	40.8
130-134	36.4260145444627	37.0	34.0	40.0	31.0	41.0
135-139	36.149340558764656	36.2	34.0	39.6	31.0	41.0
140-144	35.60205607392068	35.2	34.0	38.6	31.0	40.4
145-149	35.02206540051088	35.0	34.0	36.8	31.0	39.4
150-154	34.533910926021164	35.0	33.0	36.0	30.4	38.0
155-159	34.14962440852209	35.0	33.0	35.0	30.0	36.6
160-164	33.90709976132107	35.0	33.0	35.0	30.0	36.0
165-169	33.78921612057182	35.0	33.0	35.0	30.0	35.6
170-174	33.58682606092067	34.8	32.8	35.0	29.8	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	4.0
28	105.0
29	1466.0
30	10251.0
31	45309.0
32	140982.0
33	280534.0
34	432058.0
35	681905.0
36	1091926.0
37	1417933.0
38	1015925.0
39	175308.0
40	1134.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.74799616230141	27.0897515316799	27.725294815329644	22.43695749068905
2	22.356841755369377	27.250474046430114	27.68793769027959	22.70474650792092
3	22.463096146436907	27.311892332912795	27.63076882398713	22.594242696663166
4	22.748506092724238	27.341336093253055	27.24839655211489	22.66176126190782
5	22.467270021379303	27.585271698483805	27.26998360668122	22.677474673455666
6	22.555412439280506	27.661685716659996	27.286018085532334	22.49688375852717
7	22.914592320070106	27.43038505412817	27.090922483021206	22.564100142780518
8	22.5754508162664	27.55320274078159	27.107561323854924	22.763785119097083
9	22.50880102137175	27.58334529466424	27.27260880404318	22.63524487992083
10-14	22.563858397987474	27.4803997854515	27.379939714892238	22.575802101668792
15-19	22.431873295510346	27.36350484622765	27.5624985835266	22.642123274735404
20-24	22.46093177508669	27.383750972645064	27.400624003747044	22.754693248521203
25-29	22.49501023638108	27.41726662184315	27.37268359383853	22.715039547937238
30-34	22.480917270399107	27.400805312341824	27.39269930725008	22.72557811000899
35-39	22.466431469128434	27.41876241775011	27.408170974004882	22.706635139116575
40-44	22.59385741589925	27.35880970907525	27.29371992354821	22.753612951477287
45-49	22.761401666528165	27.35733657674264	27.224569580950508	22.656692175778684
50-54	22.66521745699587	27.381968104796368	27.243127271079015	22.709687167128752
55-59	22.64879014285606	27.384525311435283	27.270274455885353	22.6964100898233
60-64	22.59903604263774	27.417395048764458	27.28264499021689	22.700923918380912
65-69	22.533965143422652	27.464637269492563	27.290951190215377	22.710446396869404
70-74	22.547963677844844	27.450068368449283	27.28495667480037	22.7170112789055
75-79	22.515826729419587	27.484656760166505	27.2842049995845	22.715311510829412
80-84	22.481472527970627	27.519683314321114	27.23442445852944	22.764419699178823
85-89	21.75290282614772	27.441527978182535	28.55983939080312	22.245729804866624
90-94	22.135779643162504	27.40018044999126	27.72186515378402	22.742174753062216
95-99	22.298123896587242	27.65766475486451	27.317380416117555	22.726830932430698
100-104	22.282006256729083	27.616851387947587	27.400888602668154	22.700253752655172
105-109	22.22285016152077	27.498965274040867	27.539050720646614	22.739133843791752
110-114	22.270396936057267	27.517814108316784	27.4279717467289	22.78381720889704
115-119	22.310886848142374	27.558604399233023	27.354519770813056	22.77598898181154
120-124	22.30383087936928	27.534538976398608	27.396067654473764	22.765562489758345
125-129	22.395571703538504	27.553903695675764	27.31211681016	22.738407790625732
130-134	22.598437752764582	27.50783806027889	27.21271520981004	22.681008977146483
135-139	22.525112612869858	27.55407445649051	27.309783047902865	22.611029882736762
140-144	22.47904066075025	27.593212092716435	27.338242717473836	22.58950452905948
145-149	22.438993516791303	27.648661536134245	27.364558762784274	22.547786184290175
150-154	22.37567999993507	27.712597422842034	27.41361836689837	22.49810421032453
155-159	22.285926550605105	27.802045471116717	27.447035572192867	22.46499240608531
160-164	22.27426259452971	27.84752886174146	27.476790041131615	22.401418502597213
165-169	22.188410378555616	27.954719536514677	27.5328069976021	22.32406308732761
170-174	22.115800045460084	28.020851494648895	27.551875254503567	22.311473205387458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	2.0
14	8.0
15	22.0
16	48.0
17	136.5
18	335.0
19	663.0
20	1297.0
21	2424.5
22	4203.0
23	6804.5
24	10493.0
25	15505.5
26	21837.5
27	29662.0
28	39038.0
29	49783.0
30	61276.5
31	73572.5
32	86488.5
33	99623.5
34	112540.0
35	125526.0
36	139020.5
37	151944.5
38	164436.5
39	177102.0
40	189200.5
41	199044.0
42	208089.5
43	216959.5
44	223908.5
45	228532.0
46	230948.5
47	229865.5
48	224916.0
49	217452.0
50	206745.0
51	193265.0
52	177669.5
53	160663.0
54	143445.0
55	126425.0
56	110288.5
57	95858.0
58	83637.0
59	73108.0
60	63649.0
61	55364.5
62	47990.5
63	41324.0
64	35494.5
65	30102.5
66	25140.5
67	20436.0
68	16349.5
69	12941.5
70	9880.5
71	7426.0
72	5387.5
73	3748.0
74	2590.5
75	1763.5
76	1121.0
77	692.0
78	427.5
79	255.0
80	132.0
81	61.5
82	37.5
83	15.5
84	5.5
85	3.5
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1938.0
92-93	4259.0
94-95	6293.0
96-97	8088.0
98-99	9942.0
100-101	11804.0
102-103	13116.0
104-105	14865.0
106-107	16060.0
108-109	17153.0
110-111	18246.0
112-113	19067.0
114-115	20413.0
116-117	21204.0
118-119	23061.0
120-121	22931.0
122-123	23505.0
124-125	24288.0
126-127	25741.0
128-129	58205.0
130-131	85879.0
132-133	79714.0
134-135	82708.0
136-137	90621.0
138-139	95468.0
140-141	100086.0
142-143	86768.0
144-145	93207.0
146-147	97729.0
148-149	94921.0
150-151	85747.0
152-153	81440.0
154-155	77721.0
156-157	80463.0
158-159	84841.0
160-161	84088.0
162-163	86745.0
164-165	89089.0
166-167	90500.0
168-169	95133.0
170-171	94594.0
172-173	177461.0
174-175	2899738.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.01080432273467
#Duplication Level	Percentage of total
1	85.52425036613998
2	11.017914658465381
3	2.190172318621222
4	0.5607471605077301
5	0.22809924774368726
6	0.13711524661028157
7	0.09615141486542103
8	0.07786163802220616
9	0.051679090437833704
>10	0.11600885858624878
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.8886311956546375E-5	0.0	0.0	0.0
2	0.0	0.0	1.8886311956546375E-5	0.0	0.0	0.0
3	0.0	0.0	1.8886311956546375E-5	0.0	0.0	0.0
4	1.8886311956546375E-5	0.0	1.8886311956546375E-5	0.0	0.0	0.0
5	1.8886311956546375E-5	1.8886311956546375E-5	1.8886311956546375E-5	0.0	0.0	0.0
6	1.8886311956546375E-5	1.8886311956546375E-5	1.8886311956546375E-5	0.0	0.0	0.0
7	1.8886311956546375E-5	3.777262391309275E-5	1.8886311956546375E-5	1.8886311956546375E-5	0.0	0.0
8	1.8886311956546375E-5	3.777262391309275E-5	1.8886311956546375E-5	3.777262391309275E-5	0.0	0.0
9	1.8886311956546375E-5	3.777262391309275E-5	1.8886311956546375E-5	3.777262391309275E-5	0.0	0.0
10-14	1.8886311956546375E-5	5.288167347832985E-5	1.8886311956546375E-5	3.777262391309275E-5	0.0	0.0
15-19	1.8886311956546375E-5	9.065429739142258E-5	1.8886311956546375E-5	7.176798543487623E-5	0.0	0.0
20-24	1.8886311956546375E-5	1.1331787173927824E-4	3.02180991304742E-5	1.1331787173927824E-4	0.0	0.0
25-29	1.8886311956546375E-5	1.1331787173927824E-4	8.687703500011331E-5	1.208723965218968E-4	3.777262391309275E-6	0.0
30-34	1.208723965218968E-4	1.4731323326106172E-4	1.0198608456535043E-4	1.4353597086975246E-4	4.910441108702057E-5	0.0
35-39	4.94821373261515E-4	1.775313323915359E-4	1.1331787173927824E-4	1.5486775804368028E-4	1.0576334695665969E-4	0.0
40-44	0.0010274153704361226	1.8886311956546373E-4	1.51090495652371E-4	1.6997680760891736E-4	1.1331787173927824E-4	1.8886311956546375E-5
45-49	0.0013295963617408646	1.8886311956546373E-4	1.6997680760891736E-4	1.8886311956546373E-4	1.4731323326106172E-4	1.8886311956546375E-5
50-54	0.0017073226008717923	2.228584810872472E-4	1.92640381956773E-4	2.228584810872472E-4	2.379675306524843E-4	1.8886311956546375E-5
55-59	0.0019452901315242764	2.7574015456557704E-4	2.1530395630462866E-4	2.2663574347855648E-4	2.7574015456557704E-4	1.8886311956546375E-5
60-64	0.0021681486126115235	2.984037289134327E-4	2.417447930437936E-4	2.2663574347855648E-4	3.286218280439069E-4	3.399536152178348E-5
65-69	0.0037432670297874913	3.626171895656904E-4	2.455220554351029E-4	2.379675306524843E-4	3.7394897673961815E-4	3.777262391309275E-5
70-74	0.03140415952134531	3.928352886961646E-4	2.6063110500034E-4	2.455220554351029E-4	4.3438517500056663E-4	4.9104411087020574E-5
75-79	0.043317645103534766	3.9661255108747385E-4	2.644083673916492E-4	2.455220554351029E-4	5.288167347832984E-4	5.665893586963912E-5
80-84	0.043457403812013205	4.154988630440203E-4	2.681856297829585E-4	2.455220554351029E-4	7.516752158705457E-4	5.665893586963912E-5
85-89	0.04354805810940463	4.306079126092573E-4	2.832946793481956E-4	2.455220554351029E-4	7.932251021749477E-4	5.665893586963912E-5
90-94	0.04368026229310045	4.6460327413104085E-4	2.832946793481956E-4	2.455220554351029E-4	7.932251021749477E-4	5.665893586963912E-5
95-99	0.043808689214404964	4.721577989136593E-4	2.9840372891343274E-4	2.6063110500034E-4	7.97002364566257E-4	5.665893586963912E-5
100-104	0.04395977971005734	4.721577989136593E-4	3.0973551608736057E-4	2.644083673916492E-4	8.121114141314941E-4	5.665893586963912E-5
105-109	0.04405421126984007	4.721577989136593E-4	3.2106730326128834E-4	2.644083673916492E-4	8.234432013054219E-4	5.665893586963912E-5
110-114	0.04415619735440542	4.721577989136593E-4	3.3617635282652536E-4	2.7574015456557704E-4	8.309977260880406E-4	5.665893586963912E-5
115-119	0.04428840153810125	4.910441108702058E-4	3.3995361521783473E-4	2.832946793481956E-4	8.309977260880406E-4	5.665893586963912E-5
120-124	0.04469634587636265	4.910441108702058E-4	3.3995361521783473E-4	2.832946793481956E-4	8.612158252185146E-4	5.665893586963912E-5
125-129	0.04530826238375475	5.099304228267521E-4	3.3995361521783473E-4	2.832946793481956E-4	8.763248747837517E-4	5.665893586963912E-5
130-134	0.045995724138973036	5.401485219572262E-4	3.3995361521783473E-4	2.832946793481956E-4	9.329838106533908E-4	5.665893586963912E-5
135-139	0.04683049912745239	5.477030467398448E-4	3.4373087760914404E-4	3.0973551608736057E-4	9.669791721751744E-4	8.309977260880404E-5
140-144	0.04756328803136639	5.477030467398448E-4	3.588399271743811E-4	3.2106730326128834E-4	0.0010085290584795762	9.443155978273186E-5
145-149	0.04806944119180183	5.62812096305082E-4	3.588399271743811E-4	3.286218280439069E-4	0.0010236381080448134	9.443155978273186E-5
150-154	0.04839428575745443	5.665893586963912E-4	3.7772623913092746E-4	3.512854023917626E-4	0.0010500789447839783	9.443155978273186E-5
155-159	0.04893065701702034	5.968074578268655E-4	3.9661255108747385E-4	3.588399271743811E-4	0.001087851568697071	9.443155978273186E-5
160-163	0.04932632525250999	6.185267165768937E-4	4.0133412907661045E-4	3.588399271743811E-4	0.0011379002953819189	9.443155978273186E-5
>>END_MODULE
