##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293793
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5066011
Total Bases	839.5 Mbp
Sequences flagged as poor quality	0
Sequence length	90-179
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.564684916791535	39.0	37.0	39.0	35.0	39.0
2	39.18425838396324	40.0	38.0	41.0	36.0	41.0
3	39.29355443562993	40.0	39.0	41.0	36.0	41.0
4	39.347882584542354	40.0	39.0	41.0	36.0	41.0
5	39.37421572910126	40.0	39.0	41.0	36.0	41.0
6	39.36439952459637	40.0	39.0	41.0	36.0	41.0
7	39.41262839737221	40.0	39.0	41.0	37.0	41.0
8	39.392653312438526	40.0	39.0	41.0	37.0	41.0
9	39.38698968478355	40.0	39.0	41.0	37.0	41.0
10-14	39.321423739506294	40.0	39.0	41.0	36.0	41.0
15-19	39.17390992637008	40.0	38.4	41.0	36.0	41.0
20-24	39.00289975683037	40.0	38.0	41.0	35.6	41.0
25-29	38.80359612326148	40.0	38.0	40.8	35.0	41.0
30-34	38.673377693021195	40.0	37.8	40.8	34.8	41.0
35-39	38.280980124204234	39.8	36.8	40.0	34.0	41.0
40-44	37.99752163980694	39.0	35.8	40.0	34.0	41.0
45-49	37.37546953609062	38.2	35.0	40.0	33.0	41.0
50-54	36.6587204804727	36.6	35.0	39.6	33.0	40.8
55-59	35.93608280755806	35.6	34.0	38.6	32.6	40.0
60-64	35.187614002417284	35.0	33.8	36.8	31.8	39.2
65-69	34.80774992395398	35.0	33.8	36.0	32.0	38.6
70-74	34.59669704625592	35.0	34.0	35.4	32.0	37.0
75-79	34.464079371323905	35.0	33.2	35.0	32.0	37.0
80-84	34.41250392073764	35.0	33.0	35.0	32.0	37.0
85-89	34.27643058808992	34.8	32.8	35.0	31.4	37.6
90-94	37.42120161892307	39.6	35.6	40.2	31.4	40.2
95-99	37.993987233201054	40.0	36.0	41.0	32.0	41.0
100-104	37.89995372119748	40.0	36.0	41.0	32.0	41.0
105-109	37.76517037288559	40.0	36.0	41.0	32.0	41.0
110-114	37.58010255351968	39.6	35.0	40.4	31.0	41.0
115-119	37.37059648222621	39.0	35.0	40.0	31.0	41.0
120-124	37.11308654082915	38.6	34.6	40.0	31.0	41.0
125-129	36.807997506204536	38.0	34.0	39.8	30.8	40.8
130-134	36.49534169729238	37.8	34.0	40.0	30.0	40.8
135-139	36.25328539823132	36.8	34.0	40.0	30.4	41.0
140-144	36.032918656913765	36.0	34.0	39.6	31.0	41.0
145-149	35.50288235204656	35.0	34.0	38.4	31.0	40.2
150-154	34.943637669696145	35.0	33.2	36.8	30.0	39.4
155-159	34.451724910271466	35.0	33.0	36.0	30.0	37.8
160-164	34.08119742772044	35.0	33.0	35.0	30.0	36.6
165-169	33.84137321681473	35.0	33.0	35.0	30.0	36.0
170-174	33.72385560281707	35.0	33.0	35.0	30.0	35.4
175-179	33.51896431059655	34.8	32.8	35.0	29.8	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
27	1.0
28	115.0
29	1636.0
30	9832.0
31	41594.0
32	136518.0
33	288326.0
34	431779.0
35	653001.0
36	1032319.0
37	1347214.0
38	963265.0
39	159644.0
40	767.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.145925068066376	27.84561660051666	27.865494172831447	22.142964158585524
2	22.1165923248094	28.024455533160115	28.5061362875051	21.352815854525385
3	20.558680192364367	26.922503721369733	29.87421464343445	22.64460144283145
4	21.37273290563325	26.086184968804844	30.112607335436103	22.4284747901258
5	22.264618059455458	26.816325507386384	28.387759126460644	22.531297306697518
6	22.375612686194327	26.946506827561173	28.030436570311434	22.647443915933067
7	22.394384852302927	27.25724440787831	27.61713308557759	22.731237654241177
8	22.484337282331207	27.369443137806055	27.49913097306737	22.647088606795364
9	22.69318009771396	27.336083557655126	27.304875571726946	22.66586077290397
10-14	22.622477527190526	27.54720429939848	27.199317174794924	22.63100099861607
15-19	22.54717172939419	27.47149581791275	27.374531954233817	22.606800498459243
20-24	22.424136070766526	27.350055102525438	27.565250055714447	22.66055877099359
25-29	22.491471100240407	27.37642298842225	27.383264663262675	22.74884124807467
30-34	22.516232199258944	27.430473404025374	27.336482293465213	22.716812103250465
35-39	22.504463571042386	27.425191141511533	27.348949696319252	22.721395591126825
40-44	22.5456083691883	27.398231073718556	27.346687561475886	22.70947299561726
45-49	22.76763315357981	27.31214756541192	27.213474269992705	22.70674501101557
50-54	22.69298270374857	27.37733889642166	27.26992104833566	22.659757351494108
55-59	22.638809903886905	27.35786795567558	27.287362779117537	22.71595936131998
60-64	22.619390285571825	27.406371600851244	27.27454796288441	22.69969015069253
65-69	22.58204334732001	27.42432260806382	27.298712932127465	22.694921112488704
70-74	22.53219347530039	27.470295662603178	27.27951439505362	22.71799646704281
75-79	22.519035193567483	27.454871298147598	27.29027631404669	22.73581719423823
80-84	22.48961559696574	27.489675802125184	27.291681759080273	22.72902684182881
85-89	22.492450174308743	27.49530547801811	27.229905343671778	22.782339004001372
90-94	21.740968216197224	27.43108996284501	28.565665492625087	22.26227632833268
95-99	22.114561769140547	27.408652233091928	27.728020902230348	22.748765095537173
100-104	22.270025486262792	27.698871581810458	27.32322948211032	22.707873449816425
105-109	22.260024765468387	27.63519486490103	27.411339887435076	22.693440482195506
110-114	22.18597539651017	27.51405159252458	27.56324694837644	22.73672606258881
115-119	22.23021087197617	27.530749456142946	27.424921005442375	22.814118666438507
120-124	22.27411545774069	27.56239183202019	27.392187855204742	22.771304855034376
125-129	22.27637966308416	27.534360487805813	27.419580592514052	22.769679256595975
130-134	22.37335224273739	27.56057304937749	27.327968829837285	22.738105878047836
135-139	22.5780438428735	27.516479052973775	27.2218021204629	22.683674983689826
140-144	22.500304229712256	27.58815392857668	27.30241530053587	22.609126541175193
145-149	22.454402241797418	27.62319813899082	27.347207358483814	22.575192260727945
150-154	22.433633131961717	27.68200789044822	27.355640312624484	22.528718664965584
155-159	22.348976644760704	27.748877805608053	27.40912567823065	22.493019871400595
160-164	22.267282905684706	27.847404746418793	27.432462816752167	22.452849531144334
165-169	22.23607022447835	27.872215264717866	27.48990075551005	22.40181375529374
170-174	22.14870026129501	27.982005567026892	27.54524157103098	22.324052600647118
175-179	22.115877823498607	28.04863545289564	27.560734781971984	22.27475194163377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	2.5
14	6.5
15	25.5
16	62.5
17	142.5
18	325.0
19	734.5
20	1506.0
21	2686.5
22	4505.5
23	7318.0
24	11278.0
25	16453.0
26	22906.0
27	30613.5
28	39615.5
29	49974.0
30	61271.0
31	72842.5
32	84342.0
33	96254.5
34	108284.5
35	120381.5
36	132885.5
37	144818.0
38	156265.0
39	167405.0
40	178412.5
41	188273.0
42	197205.5
43	205122.5
44	211240.0
45	215830.5
46	218277.0
47	217753.0
48	214004.0
49	207287.0
50	197192.0
51	184540.0
52	169419.5
53	153237.0
54	136761.0
55	120504.5
56	106045.0
57	92570.5
58	80598.0
59	70370.0
60	61251.0
61	53646.5
62	46716.5
63	40156.0
64	34496.5
65	29079.5
66	24034.0
67	19685.5
68	15857.0
69	12414.5
70	9530.5
71	7195.0
72	5189.0
73	3611.0
74	2473.5
75	1687.5
76	1089.5
77	689.5
78	414.5
79	227.0
80	115.0
81	67.0
82	34.5
83	13.0
84	4.0
85	3.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1677.0
92-93	3930.0
94-95	5725.0
96-97	7571.0
98-99	9008.0
100-101	10700.0
102-103	11650.0
104-105	13287.0
106-107	14639.0
108-109	15546.0
110-111	16768.0
112-113	17393.0
114-115	18320.0
116-117	19323.0
118-119	20465.0
120-121	20860.0
122-123	21439.0
124-125	22190.0
126-127	22970.0
128-129	23847.0
130-131	24123.0
132-133	26460.0
134-135	97964.0
136-137	99280.0
138-139	88138.0
140-141	89316.0
142-143	97474.0
144-145	86172.0
146-147	90427.0
148-149	95401.0
150-151	87892.0
152-153	78184.0
154-155	82076.0
156-157	79682.0
158-159	76655.0
160-161	84690.0
162-163	89274.0
164-165	72273.0
166-167	77982.0
168-169	79973.0
170-171	79578.0
172-173	77641.0
174-175	82220.0
176-177	91710.0
178-179	2834118.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.00451761774357
#Duplication Level	Percentage of total
1	85.45725086573827
2	11.195591509078119
3	2.1329829300865355
4	0.5481114228300418
5	0.20461212922051616
6	0.14581045636992293
7	0.10170423598214508
8	0.07168392455073994
9	0.043218665535354396
>10	0.0990338606083561
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.9478793078025293E-5	0.0	0.0	0.0	0.0	0.0
2	2.3687275846815176E-4	0.0	0.0	0.0	0.0	0.0
3	2.3687275846815176E-4	0.0	5.921818961703794E-5	0.0	0.0	0.0
4	2.3687275846815176E-4	0.0	5.921818961703794E-5	0.0	0.0	0.0
5	2.3687275846815176E-4	0.0	5.921818961703794E-5	0.0	1.9739396539012647E-5	0.0
6	2.3687275846815176E-4	0.0	5.921818961703794E-5	0.0	1.9739396539012647E-5	0.0
7	2.3687275846815176E-4	0.0	7.895758615605059E-5	0.0	3.9478793078025293E-5	0.0
8	2.3687275846815176E-4	0.0	7.895758615605059E-5	0.0	3.9478793078025293E-5	0.0
9	2.3687275846815176E-4	0.0	7.895758615605059E-5	0.0	3.9478793078025293E-5	0.0
10-14	2.447685170837568E-4	0.0	7.895758615605059E-5	1.9739396539012647E-5	5.527031030923541E-5	0.0
15-19	2.566121550071644E-4	1.9739396539012647E-5	8.685334477165565E-5	1.9739396539012647E-5	7.895758615605059E-5	0.0
20-24	2.6056003431496695E-4	3.1583034462420235E-5	9.869698269506324E-5	1.9739396539012647E-5	1.0659274131066829E-4	0.0
25-29	2.9609094808518967E-4	5.921818961703795E-5	9.869698269506324E-5	1.9739396539012647E-5	1.6581093092770625E-4	0.0
30-34	3.7899641354904287E-4	7.106182754044554E-5	9.869698269506324E-5	1.9739396539012647E-5	2.092376033135341E-4	0.0
35-39	7.264097926356653E-4	1.184363792340759E-4	1.1054062061847082E-4	1.9739396539012647E-5	2.8819518946958465E-4	0.0
40-44	0.0011054062061847084	1.184363792340759E-4	1.184363792340759E-4	1.9739396539012647E-5	3.5136125839442517E-4	0.0
45-49	0.0014054450335777007	1.184363792340759E-4	1.184363792340759E-4	4.737455169363035E-5	4.2242308593487066E-4	1.9739396539012647E-5
50-54	0.0016502135506614574	1.184363792340759E-4	1.184363792340759E-4	9.869698269506324E-5	4.737455169363036E-4	1.9739396539012647E-5
55-59	0.0018515553953593865	1.184363792340759E-4	1.3028001715748348E-4	9.869698269506324E-5	5.369115858611439E-4	3.553091377022277E-5
60-64	0.0020331578435183024	1.5396729300429864E-4	1.5791517231210117E-4	1.263321378496809E-4	5.724424996313667E-4	3.9478793078025293E-5
65-69	0.0021279069469055632	1.7765456885111383E-4	1.9739396539012648E-4	1.5791517231210117E-4	6.790352409420351E-4	3.9478793078025293E-5
70-74	0.0033556974116321497	1.8949820677452144E-4	2.01341844697929E-4	1.7765456885111383E-4	7.303576719434679E-4	4.342667238582782E-5
75-79	0.026198127086577584	2.68455792930572E-4	2.1713336192913908E-4	1.8160244815891636E-4	8.014194994839134E-4	5.921818961703795E-5
80-84	0.03592570170100302	3.276739825476099E-4	2.1713336192913908E-4	2.092376033135341E-4	8.961686028711741E-4	5.921818961703795E-5
85-89	0.03599676352854346	3.9478793078025296E-4	2.1713336192913908E-4	2.487163963915594E-4	0.0011054062061847082	5.921818961703795E-5
90-94	0.0360480859595449	4.2637096524267314E-4	2.1713336192913908E-4	2.921430687773872E-4	0.0011054062061847082	5.921818961703795E-5
95-99	0.036194157493933586	4.3426672385827816E-4	2.2502912054474418E-4	3.118824653163998E-4	0.001133041361339326	5.921818961703795E-5
100-104	0.03628101083870525	4.3426672385827816E-4	2.368727584681518E-4	3.3556974116321503E-4	0.0011448849992627336	5.921818961703795E-5
105-109	0.036379707821400306	4.69797637628501E-4	2.368727584681518E-4	3.434654997788201E-4	0.0011448849992627336	5.921818961703795E-5
110-114	0.036573153907482635	4.895370341675136E-4	2.368727584681518E-4	4.2637096524267314E-4	0.0011448849992627336	5.921818961703795E-5
115-119	0.03670343392464011	5.013806720909213E-4	2.368727584681518E-4	4.461103617816859E-4	0.0011448849992627336	5.921818961703795E-5
120-124	0.03696794183826289	5.44807344476749E-4	2.5266427569936193E-4	4.737455169363036E-4	0.0011448849992627336	5.921818961703795E-5
125-129	0.037568019493048874	5.527031030923541E-4	2.724036722383745E-4	4.737455169363036E-4	0.0011883116716485615	6.711394823264299E-5
130-134	0.03814835775129584	5.645467410157617E-4	2.842473101617821E-4	4.737455169363036E-4	0.0012475298612655995	7.895758615605059E-5
135-139	0.03892608997493294	5.803382582469719E-4	2.960909480851897E-4	4.895370341675136E-4	0.0012830607750358221	7.895758615605059E-5
140-144	0.03950248035387211	6.277128099406021E-4	3.553091377022276E-4	4.934849134753162E-4	0.0013185916888060448	7.895758615605059E-5
145-149	0.040090714370734686	6.869309995576403E-4	3.8294429285684535E-4	5.092764307065263E-4	0.0013343832060372551	7.895758615605059E-5
150-154	0.04054472049113198	7.145661547122578E-4	3.9478793078025296E-4	5.211200686299339E-4	0.001393601395654293	7.895758615605059E-5
155-159	0.04101451812876048	7.303576719434679E-4	3.9478793078025296E-4	5.329637065533415E-4	0.0014370280680401208	7.895758615605059E-5
160-164	0.04155142971462163	7.303576719434679E-4	4.145273273192656E-4	5.44807344476749E-4	0.0014567674645791335	7.895758615605059E-5
165-168	0.04202024038242318	7.303576719434679E-4	4.2933187472352503E-4	5.625728013618604E-4	0.0014804547404259486	7.895758615605059E-5
>>END_MODULE
