##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293798
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5191076
Total Bases	890 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.31496533666623	39.0	39.0	39.0	37.0	39.0
2	40.08685732977132	41.0	40.0	41.0	38.0	41.0
3	40.13517120535319	41.0	40.0	41.0	38.0	41.0
4	40.14860136896474	41.0	40.0	41.0	38.0	41.0
5	40.15128751727002	41.0	40.0	41.0	38.0	41.0
6	40.1387419486827	41.0	40.0	41.0	38.0	41.0
7	40.164284052092476	41.0	40.0	41.0	38.0	41.0
8	40.14768402543134	41.0	40.0	41.0	38.0	41.0
9	40.12629886366526	41.0	40.0	41.0	38.0	41.0
10-14	40.06141647704638	41.0	40.0	41.0	38.0	41.0
15-19	39.945538420165676	41.0	40.0	41.0	38.0	41.0
20-24	39.76859718486109	41.0	40.0	41.0	37.4	41.0
25-29	39.53310011257783	40.4	39.0	41.0	36.6	41.0
30-34	39.30925796501534	40.0	38.6	41.0	35.4	41.0
35-39	39.16256883929266	40.0	38.0	41.0	35.0	41.0
40-44	38.668296052687346	39.8	36.8	41.0	35.0	41.0
45-49	37.94689767593462	38.8	35.2	41.0	35.0	41.0
50-54	37.16178634256173	36.8	35.0	39.6	34.0	41.0
55-59	36.42019835579367	35.8	35.0	38.6	34.0	40.6
60-64	35.65772745380727	35.0	34.8	36.8	33.6	39.2
65-69	35.337361656812575	35.0	34.8	36.0	33.6	38.4
70-74	35.175792764351755	35.0	35.0	35.4	34.0	37.0
75-79	35.064947228667044	35.0	35.0	35.0	33.0	36.6
80-84	35.02901421593519	35.0	35.0	35.0	33.0	36.0
85-89	34.862354895208625	35.0	34.6	35.0	32.6	37.0
90-94	39.64790023604597	40.6	39.8	40.6	37.8	40.6
95-99	39.909100479248174	41.0	40.0	41.0	38.0	41.0
100-104	39.78746868935465	41.0	40.0	41.0	37.0	41.0
105-109	39.637028977705974	41.0	39.6	41.0	36.4	41.0
110-114	39.45155330492675	40.8	39.0	41.0	35.6	41.0
115-119	39.175692937504934	40.0	38.2	41.0	35.0	41.0
120-124	38.90562302467366	40.0	38.0	41.0	35.0	41.0
125-129	38.36051618007322	39.6	36.6	40.6	34.6	41.0
130-134	38.048927883362566	39.0	35.4	41.0	34.0	41.0
135-139	37.63421713102143	38.2	35.0	40.8	34.0	41.0
140-144	36.97434415732695	36.6	35.0	39.4	34.0	41.0
145-149	36.22345297268598	35.4	35.0	38.0	34.0	40.2
150-154	35.57302595848478	35.0	35.0	36.4	34.0	38.6
155-159	35.09773234724433	35.0	35.0	35.6	33.8	37.0
160-164	34.807225188524924	35.0	35.0	35.0	33.0	36.0
165-169	34.64324403014284	35.0	35.0	35.0	33.0	36.0
170-174	34.55560735740743	35.0	35.0	35.0	33.0	35.2
175-178	34.2610065150308	35.0	34.5	35.0	32.25	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	3.0
29	32.0
30	347.0
31	2413.0
32	12214.0
33	44394.0
34	123823.0
35	295935.0
36	774248.0
37	1763547.0
38	1878649.0
39	294510.0
40	961.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.236507421582733	26.957609559174244	27.96065786746332	22.845225151779708
2	23.151674142316544	27.823826890609958	27.542844681911806	21.48165428516169
3	21.16672920989791	26.685007116058408	28.935600249351	23.212663424692686
4	21.735744188680727	26.107053720654445	29.441275758628848	22.71592633203598
5	22.815000204196586	26.456557368838368	27.578193807988942	23.150248618976104
6	22.977124588428293	26.676415448357915	27.222467943062284	23.12399202015151
7	22.834841947989204	27.150902818606394	27.115264735095383	22.898990498309022
8	22.972770963091275	27.051944529419337	26.931564862467823	23.04371964502157
9	23.175734664643706	27.11869369664401	26.741180441203326	22.964391197508956
10-14	22.98800479900506	27.195768276172416	26.813716462637032	23.002510462185487
15-19	22.95476698857809	27.22335407919283	26.905211944498596	22.916666987730483
20-24	22.90644945286873	27.020197739351147	27.024046652370338	23.049306155409784
25-29	22.879553294923827	27.10582931168798	26.900037680049376	23.114579713338816
30-34	22.93213198959137	27.138820545104714	26.933953577254506	22.995093888049414
35-39	22.927924769354178	27.071713070662035	26.92786235454846	23.072499805435328
40-44	22.909454610181008	27.136890309446443	26.961412239004012	22.992242841368533
45-49	23.0769690137459	27.0435955859633	26.842061260517085	23.03737413977372
50-54	23.015378699907302	27.07489930796621	26.890794894931226	23.018927097195263
55-59	22.987153337766582	27.05692615557931	26.89420459265093	23.061715914003187
60-64	22.95753712717749	27.128375697061653	26.919243717487472	22.99484345827339
65-69	22.97342593327472	27.039272782752555	26.88175245363389	23.105548830338833
70-74	22.89923707531926	27.106700036755388	26.949661303359846	23.044401584565513
75-79	22.92465377120273	27.080616812391114	26.93615735928351	23.058572057122646
80-84	22.896335942683173	27.086446047023777	26.919921804265627	23.09729620602742
85-89	22.88372198750317	27.071235327704702	26.918561777943534	23.1264809068486
90-94	22.26360274085566	26.8672637915581	28.18045687606884	22.688676591517403
95-99	22.901171377250076	27.130805149306525	27.034121524531862	22.933901948911533
100-104	23.00146306143664	27.172287316073696	26.84444394991894	22.981805672570722
105-109	22.859194816916037	27.213224070754016	27.030900739300833	22.896680373029113
110-114	22.902406026283405	27.049236468105313	27.042026061966368	23.006331443644907
115-119	22.902859658758977	27.094993904001267	26.929061243488604	23.07308519375115
120-124	22.853387753273893	27.115885923906625	27.002623717886625	23.028102604932858
125-129	22.835322843051728	27.13509695897629	27.00652998455835	23.023050213413626
130-134	22.94804097928144	27.101613887959104	26.936047583272032	23.014297549487427
135-139	22.983831712011263	27.09984540086704	26.897088545387156	23.019234341734542
140-144	23.004368610450722	27.142179870020744	26.956959677148827	22.896491842379703
145-149	22.971190142213263	27.09893235791643	26.944285649863716	22.985591850006596
150-154	22.875671646204843	27.24996769460749	27.00736911372774	22.866991545459925
155-159	22.878132596145	27.21908330274957	27.044680944972793	22.858103156132636
160-164	22.845843821532664	27.277595366534683	27.033292568674806	22.84326824325785
165-169	22.744902227438637	27.30678884637301	27.104244424149016	22.844064502039338
170-174	22.757735083635307	27.387021112082344	27.125322031117864	22.729921773164484
175-178	22.719001173594194	27.40394831074628	27.098411335501638	22.77863918015789
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	1.0
14	2.0
15	3.0
16	8.5
17	19.0
18	43.5
19	109.5
20	260.0
21	482.5
22	921.5
23	1774.5
24	3097.0
25	5085.0
26	8002.5
27	12099.5
28	17740.5
29	25006.0
30	34247.5
31	45551.0
32	58822.0
33	74180.5
34	91146.5
35	109770.5
36	129775.5
37	150238.0
38	170787.5
39	190142.0
40	207977.5
41	224129.5
42	237118.0
43	245935.0
44	251487.5
45	252263.5
46	248016.5
47	240383.0
48	228559.5
49	214303.0
50	198454.0
51	181719.0
52	164222.0
53	147387.5
54	132103.5
55	117725.0
56	104356.0
57	92405.0
58	82071.5
59	72612.5
60	64417.5
61	57137.0
62	50342.5
63	44100.5
64	38581.5
65	33317.5
66	28248.5
67	23655.5
68	19498.5
69	15697.0
70	12242.0
71	9377.5
72	7060.5
73	5188.0
74	3648.5
75	2435.0
76	1595.0
77	1000.0
78	625.0
79	383.0
80	204.5
81	107.0
82	56.5
83	27.5
84	12.5
85	5.0
86	3.0
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	698.0
92-93	1407.0
94-95	2136.0
96-97	2762.0
98-99	3372.0
100-101	3875.0
102-103	4519.0
104-105	5228.0
106-107	5739.0
108-109	6312.0
110-111	6808.0
112-113	7174.0
114-115	7577.0
116-117	7960.0
118-119	8480.0
120-121	8632.0
122-123	8948.0
124-125	9611.0
126-127	9874.0
128-129	10435.0
130-131	10755.0
132-133	11927.0
134-135	54495.0
136-137	47545.0
138-139	54568.0
140-141	48328.0
142-143	48713.0
144-145	48951.0
146-147	50785.0
148-149	51804.0
150-151	50829.0
152-153	47206.0
154-155	47598.0
156-157	47420.0
158-159	48449.0
160-161	51137.0
162-163	51687.0
164-165	52748.0
166-167	52298.0
168-169	55692.0
170-171	55730.0
172-173	61716.0
174-175	61901.0
176-177	168929.0
178-179	3788318.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.5238033560839
#Duplication Level	Percentage of total
1	93.38737144752383
2	5.787376898064775
3	0.5857832914030962
4	0.10697572786536232
5	0.047772589634003555
6	0.031455566906635736
7	0.014819920650170487
8	0.01175542629750988
9	0.00647308159819545
>10	0.020216050056446194
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.9263828924870298E-5	0.0	0.0	0.0	1.9263828924870298E-5	0.0
2	2.1190211817357325E-4	0.0	1.9263828924870298E-5	0.0	1.9263828924870298E-5	0.0
3	2.1190211817357325E-4	3.8527657849740596E-5	1.9263828924870298E-5	0.0	1.9263828924870298E-5	0.0
4	2.1190211817357325E-4	3.8527657849740596E-5	3.8527657849740596E-5	0.0	1.9263828924870298E-5	0.0
5	2.1190211817357325E-4	3.8527657849740596E-5	3.8527657849740596E-5	0.0	1.9263828924870298E-5	0.0
6	2.1190211817357325E-4	3.8527657849740596E-5	3.8527657849740596E-5	0.0	1.9263828924870298E-5	0.0
7	2.5042977602331384E-4	3.8527657849740596E-5	3.8527657849740596E-5	0.0	1.9263828924870298E-5	0.0
8	2.5042977602331384E-4	3.8527657849740596E-5	5.779148677461089E-5	0.0	1.9263828924870298E-5	0.0
9	2.5042977602331384E-4	3.8527657849740596E-5	5.779148677461089E-5	1.9263828924870298E-5	1.9263828924870298E-5	0.0
10-14	2.5042977602331384E-4	4.623318941968872E-5	5.779148677461089E-5	4.623318941968872E-5	1.9263828924870298E-5	0.0
15-19	2.61988073378236E-4	8.476084726942931E-5	5.779148677461089E-5	6.164425255958495E-5	1.9263828924870298E-5	0.0
20-24	3.120740285828988E-4	9.631914462435149E-5	6.934978412953306E-5	8.861361305440337E-5	1.9263828924870298E-5	0.0
25-29	3.4674892064766533E-4	9.631914462435149E-5	7.705531569948119E-5	9.631914462435149E-5	1.9263828924870298E-5	0.0
30-34	3.9683487585232815E-4	1.0017191040932554E-4	8.090808148445526E-5	1.3484680247409208E-4	2.311659470984436E-5	0.0
35-39	4.931540204766796E-4	1.232885051191699E-4	1.6181616296891049E-4	1.5796339718393643E-4	5.779148677461089E-5	0.0
40-44	6.780867781554345E-4	1.3484680247409208E-4	2.1190211817357323E-4	2.080493523885992E-4	5.779148677461089E-5	0.0
45-49	7.975225174896303E-4	1.3484680247409208E-4	2.3116594709844356E-4	2.4272424445336574E-4	5.779148677461089E-5	1.155829735492218E-5
50-54	9.901608067383332E-4	1.6952169453885861E-4	2.851046680880804E-4	2.735463707331582E-4	9.246637883937742E-5	3.467489206476653E-5
55-59	0.0011481242039222696	2.1190211817357323E-4	3.197795601528469E-4	2.928101996580285E-4	1.1943573933419585E-4	3.8527657849740596E-5
60-64	0.0012482961143315953	2.1190211817357323E-4	3.4674892064766533E-4	3.3133785750776907E-4	1.3869956825906613E-4	3.8527657849740596E-5
65-69	0.001352320790525895	2.2731318131346948E-4	3.8142381271243184E-4	3.698655153575097E-4	1.6952169453885861E-4	3.8527657849740596E-5
70-74	0.002273131813134695	2.3116594709844356E-4	4.430680652720168E-4	4.160987047771984E-4	1.9649105503367702E-4	3.8527657849740596E-5
75-79	0.01703693030115529	2.3116594709844356E-4	4.623318941968871E-4	4.2765700213212056E-4	2.465770102383398E-4	3.8527657849740596E-5
80-84	0.023713773406515336	2.5042977602331384E-4	5.047123178316017E-4	4.430680652720168E-4	3.005157312279766E-4	3.8527657849740596E-5
85-89	0.02384091467741948	2.8125190230310636E-4	5.316816783264202E-4	4.6618465998186123E-4	4.2380423634714645E-4	3.8527657849740596E-5
90-94	0.02393723382204383	3.27485091722795E-4	5.817676335310829E-4	4.970067862616537E-4	4.2380423634714645E-4	3.8527657849740596E-5
95-99	0.0239719087141086	3.27485091722795E-4	6.665284808005121E-4	5.355344441113942E-4	4.2380423634714645E-4	3.8527657849740596E-5
100-104	0.024037405732453155	3.27485091722795E-4	7.08908904435227E-4	5.85620399316057E-4	4.2380423634714645E-4	3.8527657849740596E-5
105-109	0.024075933390302902	3.27485091722795E-4	7.435837964999934E-4	6.241480571657976E-4	4.2380423634714645E-4	3.8527657849740596E-5
110-114	0.024102902750797718	3.27485091722795E-4	7.821114543497341E-4	6.703812465854862E-4	4.2380423634714645E-4	3.8527657849740596E-5
115-119	0.024164547003357302	3.3904338907771723E-4	8.321974095543968E-4	6.742340123704604E-4	4.2380423634714645E-4	3.8527657849740596E-5
120-124	0.024349479761036054	3.4674892064766533E-4	8.553140042642411E-4	6.780867781554345E-4	4.2380423634714645E-4	3.8527657849740596E-5
125-129	0.02460376230284434	3.6601274957253564E-4	9.092527252538779E-4	7.204672017901492E-4	4.430680652720168E-4	5.393872098963683E-5
130-134	0.02486575037622258	4.0454040742227625E-4	9.439276173186446E-4	7.358782649300453E-4	4.815957231217575E-4	5.779148677461089E-5
135-139	0.02526258525207491	4.0454040742227625E-4	9.439276173186446E-4	7.859642201347081E-4	5.124178494015499E-4	5.779148677461089E-5
140-144	0.025574659280657802	4.0454040742227625E-4	9.70896977813463E-4	8.553140042642411E-4	5.702093361761608E-4	5.779148677461089E-5
145-149	0.0257827086330464	4.0454040742227625E-4	0.0010286884645880738	8.668723016191633E-4	5.971786966709792E-4	5.779148677461089E-5
150-154	0.0260061690485749	4.122459389922244E-4	0.0011018910145025808	8.707250674041374E-4	6.048842282409273E-4	5.779148677461089E-5
155-159	0.02620651286939355	4.5077359684196497E-4	0.0011365659065673474	9.208110226088003E-4	6.357063545207197E-4	5.779148677461089E-5
160-164	0.026599494979460903	4.815957231217575E-4	0.0011442714381372956	9.554859146735668E-4	6.357063545207197E-4	5.779148677461089E-5
165-167	0.02686019879757748	4.8159572312175743E-4	0.0011558297354922178	9.824552751683852E-4	6.485489071372999E-4	5.779148677461089E-5
>>END_MODULE
