##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293799
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5398077
Total Bases	927.7 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.38880401298462	39.0	39.0	39.0	37.0	39.0
2	40.1480716188376	41.0	40.0	41.0	38.0	41.0
3	40.18093165399456	41.0	40.0	41.0	38.0	41.0
4	40.187914140535604	41.0	40.0	41.0	38.0	41.0
5	40.18008468571308	41.0	40.0	41.0	38.0	41.0
6	40.1754041300263	41.0	40.0	41.0	38.0	41.0
7	40.193793641698704	41.0	40.0	41.0	38.0	41.0
8	40.18127196036663	41.0	40.0	41.0	38.0	41.0
9	40.16582627480119	41.0	40.0	41.0	38.0	41.0
10-14	40.10453140998173	41.0	40.0	41.0	38.0	41.0
15-19	39.991943019708685	41.0	40.0	41.0	38.0	41.0
20-24	39.82712410363913	41.0	40.0	41.0	37.6	41.0
25-29	39.58409496567018	40.6	39.2	41.0	36.8	41.0
30-34	39.358779765460916	40.0	39.0	41.0	35.8	41.0
35-39	39.204762733099216	40.0	38.2	41.0	35.0	41.0
40-44	38.70210213748341	39.8	36.8	41.0	35.0	41.0
45-49	37.97885043136658	38.8	35.4	41.0	35.0	41.0
50-54	37.18718610349574	36.8	35.0	39.6	34.2	41.0
55-59	36.42295984292184	35.6	35.0	38.4	34.0	40.6
60-64	35.64711396299089	35.0	34.8	36.8	33.6	39.2
65-69	35.32533956073617	35.0	34.8	36.0	33.6	38.0
70-74	35.16484911200784	35.0	35.0	35.4	34.0	37.0
75-79	35.04263214474339	35.0	35.0	35.0	33.8	36.2
80-84	35.00512786312608	35.0	35.0	35.0	33.0	36.0
85-89	34.83588740953491	35.0	34.6	35.0	32.6	36.2
90-94	39.65925773402317	40.6	39.8	40.6	37.8	40.6
95-99	39.94869090964159	41.0	40.0	41.0	38.0	41.0
100-104	39.835319939126656	41.0	40.0	41.0	37.4	41.0
105-109	39.69348911237966	41.0	39.8	41.0	36.8	41.0
110-114	39.51555216853965	41.0	39.0	41.0	35.8	41.0
115-119	39.245596634618074	40.0	38.4	41.0	35.0	41.0
120-124	38.97640416892319	40.0	38.0	41.0	35.0	41.0
125-129	38.42476945059707	39.6	36.8	40.6	34.6	41.0
130-134	38.113070837098235	39.0	35.6	41.0	34.2	41.0
135-139	37.681572284381005	38.2	35.0	41.0	34.8	41.0
140-144	36.99810527464373	36.6	35.0	39.4	34.0	41.0
145-149	36.245026832494624	35.4	35.0	38.0	34.0	40.2
150-154	35.595676028105416	35.0	35.0	36.4	34.0	38.6
155-159	35.12964649932115	35.0	35.0	35.6	34.0	37.0
160-164	34.84427945300445	35.0	35.0	35.0	34.0	36.0
165-169	34.68920990471042	35.0	35.0	35.0	33.0	36.0
170-174	34.603706352976225	35.0	35.0	35.0	33.0	35.4
175-178	34.32072211783589	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	1.0
29	24.0
30	315.0
31	2281.0
32	12152.0
33	44279.0
34	121601.0
35	292352.0
36	779848.0
37	1830801.0
38	1993971.0
39	319398.0
40	1054.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.2678187065505	26.93179441493702	27.903473774086585	22.89691310442589
2	23.169473129042064	27.819258598941808	27.468893089150082	21.542375182866046
3	21.26748099369461	26.58305911531088	28.909832149485826	23.239627741508688
4	21.809914901176843	26.04827608053757	29.34980364303807	22.792005375247516
5	22.865438933160828	26.445195205626003	27.485436017307645	23.203929843905524
6	23.000857527597326	26.6361150461544	27.21157923460521	23.151448191643063
7	22.853304982496546	27.112562492161562	27.107783012357917	22.926349512983975
8	23.05076418880279	26.997577100141402	26.854415007418382	23.097243703637425
9	23.25346600280063	27.061155296599143	26.68146452894244	23.003914171657797
10-14	23.038459807075743	27.157359926507162	26.756965489747554	23.047214776669545
15-19	23.025447765935905	27.150483403626886	26.86029487908379	22.963773951353417
20-24	22.965874699453156	26.96347977251899	26.982690317311146	23.087955210716704
25-29	22.93217380930283	27.04780609835688	26.85508561660013	23.164934475740157
30-34	22.97095428612819	27.075515966148682	26.90258771781136	23.050942029911763
35-39	22.990227816313105	27.0304258349779	26.854133425662507	23.125212923046483
40-44	22.953555497633694	27.063693237425106	26.938026263797276	23.044725001143927
45-49	23.123019549369157	27.002967908757135	26.786920601540142	23.08709194033357
50-54	23.061312389578735	27.03157068711691	26.842588573671698	23.064528349632656
55-59	23.029319515079166	27.006524730936594	26.84857218598401	23.115583568000233
60-64	23.02144634098402	27.072518602457873	26.86831625410308	23.037718802455025
65-69	23.011931841653983	26.99237895272705	26.851369478427223	23.14431972719174
70-74	22.94965781332871	27.06055878787946	26.88461465073581	23.10516874805602
75-79	22.96604142549282	27.035209019804647	26.88930150496186	23.109448049740678
80-84	22.965522722258317	27.007828899069057	26.87428134129987	23.152367037372752
85-89	22.943781646686404	27.03333798313733	26.86700838094751	23.155871989228757
90-94	22.33692381410212	26.769863143545624	28.154661759710457	22.738551282641797
95-99	22.970507322358845	27.05644082149039	26.991518270144276	22.98153358600649
100-104	23.076977082992897	27.08170946630079	26.81104133123671	23.03027211946961
105-109	22.926226721345973	27.14298245261318	26.97598911894328	22.954801707097573
110-114	22.98705523587781	26.972354914064155	27.003613206137366	23.036976643920667
115-119	22.980742634188758	27.016787415622527	26.8840697582897	23.118400191899013
120-124	22.912007192716878	27.050288017628958	26.964326591403097	23.073378198251067
125-129	22.931755420783084	27.06004875166227	26.949528858024717	23.058666969529927
130-134	22.999978163720826	27.034338973106248	26.890652458527686	23.07503040464524
135-139	23.053705124644814	27.005205252915697	26.889473751081265	23.051615871358226
140-144	23.04387546493696	27.077011404033286	26.92190568031409	22.957207450715664
145-149	23.03618899992816	27.043109702237793	26.88339527226994	23.037306025564103
150-154	22.93602576941508	27.14457739476232	26.980546019290504	22.938850816532092
155-159	22.955034377690513	27.128498525867396	26.988660970451683	22.92780612599041
160-164	22.913963935967868	27.19261554750123	26.991781950396245	22.901638566134658
165-169	22.84135284429677	27.208621129511123	27.043148204412944	22.90687782177916
170-174	22.840463294298686	27.28425137288656	27.079184721237855	22.796100611576904
175-178	22.822150614346995	27.287759217705958	27.017840557912255	22.872249610034793
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	3.5
15	5.0
16	13.0
17	25.5
18	45.5
19	92.5
20	210.0
21	467.0
22	937.0
23	1749.0
24	3025.5
25	5079.0
26	8177.0
27	12444.5
28	18031.5
29	25408.0
30	35056.0
31	46507.5
32	59678.0
33	75536.0
34	93103.5
35	112209.5
36	133392.0
37	154670.5
38	175175.0
39	195831.0
40	215035.5
41	231392.0
42	245325.5
43	255439.0
44	260721.0
45	261645.0
46	258432.0
47	250132.0
48	237556.0
49	223177.0
50	206976.5
51	189813.5
52	172368.5
53	154433.5
54	138173.5
55	123216.0
56	109578.5
57	97729.5
58	86555.0
59	76668.0
60	67962.5
61	60093.0
62	53447.0
63	47248.0
64	41187.5
65	35619.0
66	30533.5
67	25675.5
68	20998.0
69	16919.0
70	13381.0
71	10278.5
72	7647.0
73	5477.5
74	3819.5
75	2602.5
76	1725.5
77	1118.5
78	690.0
79	389.0
80	205.0
81	115.0
82	65.5
83	31.0
84	11.5
85	5.0
86	1.5
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	581.0
92-93	1348.0
94-95	1897.0
96-97	3180.0
98-99	3222.0
100-101	3645.0
102-103	4388.0
104-105	5169.0
106-107	5538.0
108-109	6247.0
110-111	6646.0
112-113	7315.0
114-115	8600.0
116-117	8305.0
118-119	8393.0
120-121	9067.0
122-123	8765.0
124-125	9195.0
126-127	9551.0
128-129	10081.0
130-131	10234.0
132-133	11349.0
134-135	52082.0
136-137	49416.0
138-139	45713.0
140-141	44733.0
142-143	46096.0
144-145	50782.0
146-147	49691.0
148-149	54558.0
150-151	48566.0
152-153	47437.0
154-155	47246.0
156-157	44325.0
158-159	51103.0
160-161	47818.0
162-163	47884.0
164-165	49511.0
166-167	53334.0
168-169	58783.0
170-171	54505.0
172-173	57618.0
174-175	61287.0
176-177	170974.0
178-179	4021899.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.2957338013134
#Duplication Level	Percentage of total
1	92.97529114500433
2	6.091673781842009
3	0.6535375717911752
4	0.14178743783423436
5	0.053922802227571606
6	0.025117597680676333
7	0.01267456790085356
8	0.013856949661093142
9	0.008605457428255529
>10	0.02353268862978851
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.705023103597818E-5	0.0	1.852511551798909E-5	5.5575346553967275E-5	1.852511551798909E-5	0.0
2	2.593516172518473E-4	0.0	1.852511551798909E-5	7.410046207195637E-5	1.852511551798909E-5	0.0
3	2.593516172518473E-4	3.705023103597818E-5	1.852511551798909E-5	7.410046207195637E-5	1.852511551798909E-5	0.0
4	2.593516172518473E-4	3.705023103597818E-5	1.852511551798909E-5	7.410046207195637E-5	1.852511551798909E-5	0.0
5	2.593516172518473E-4	3.705023103597818E-5	1.852511551798909E-5	7.410046207195637E-5	1.852511551798909E-5	0.0
6	2.593516172518473E-4	3.705023103597818E-5	1.852511551798909E-5	9.262557758994545E-5	1.852511551798909E-5	0.0
7	2.778767327698364E-4	3.705023103597818E-5	1.852511551798909E-5	9.262557758994545E-5	1.852511551798909E-5	0.0
8	2.778767327698364E-4	3.705023103597818E-5	3.705023103597818E-5	9.262557758994545E-5	1.852511551798909E-5	0.0
9	2.778767327698364E-4	3.705023103597818E-5	3.705023103597818E-5	1.2967580862592365E-4	1.852511551798909E-5	0.0
10-14	2.8899180208062983E-4	5.928036965756509E-5	3.705023103597818E-5	1.407908779367171E-4	1.852511551798909E-5	0.0
15-19	3.223370100130102E-4	1.0374064690073892E-4	4.0755254139576E-5	1.6672603966190182E-4	1.852511551798909E-5	0.0
20-24	3.5568221794539054E-4	1.2226576241872801E-4	5.5575346553967275E-5	1.7043106276549963E-4	2.593516172518473E-5	0.0
25-29	3.705023103597818E-4	1.2967580862592365E-4	6.669041586476073E-5	2.0748129380147783E-4	3.705023103597818E-5	0.0
30-34	4.2237263381015124E-4	1.2967580862592365E-4	7.780548517555418E-5	2.5194157104465166E-4	6.669041586476073E-5	0.0
35-39	5.594584886432706E-4	1.4820092414391273E-4	1.1115069310793455E-4	3.519771948417927E-4	1.0744567000433673E-4	0.0
40-44	7.372995976159659E-4	1.7784110897269527E-4	1.3338083172952146E-4	3.8161737967057525E-4	1.2967580862592365E-4	0.0
45-49	9.410758683138459E-4	2.0377627069788E-4	1.5561097035110837E-4	4.001424951885644E-4	1.7413608586909742E-4	0.0
50-54	0.0010707516769397694	2.223013862158691E-4	2.1489134000867346E-4	4.7053793415692297E-4	2.000712475942822E-4	0.0
55-59	0.0011485571621153237	2.6676166345904293E-4	2.223013862158691E-4	5.001781189857055E-4	2.2971143242306473E-4	1.1115069310793454E-5
60-64	0.0012041325086692908	2.778767327698364E-4	2.223013862158691E-4	5.335233269180859E-4	2.6676166345904293E-4	2.9640184828782546E-5
65-69	0.001263412878326856	3.1122194070221673E-4	2.85286778977032E-4	5.816886272648574E-4	2.8528677897703196E-4	3.705023103597818E-5
70-74	0.002174848561811919	3.3345207932380363E-4	3.223370100130102E-4	6.002137427828466E-4	3.2974705622020587E-4	3.705023103597818E-5
75-79	0.018091627814868148	3.3345207932380363E-4	3.3345207932380363E-4	6.52084066233216E-4	3.519771948417927E-4	3.705023103597818E-5
80-84	0.025368293190334255	3.445671486345971E-4	3.593872410489883E-4	7.150694589943789E-4	4.48307795535336E-4	3.705023103597818E-5
85-89	0.02545721374482061	3.8902742587777094E-4	3.8902742587777094E-4	7.410046207195636E-4	6.446740200260203E-4	3.705023103597818E-5
90-94	0.025572069461032142	4.001424951885644E-4	4.446027724317382E-4	7.410046207195636E-4	6.483790431296182E-4	3.705023103597818E-5
95-99	0.025620234761378913	4.186676107065535E-4	5.112931882964991E-4	7.706448055483461E-4	6.483790431296182E-4	3.705023103597818E-5
100-104	0.025657284992414892	4.2978268001734693E-4	5.187032345036946E-4	7.928749441699333E-4	6.52084066233216E-4	3.705023103597818E-5
105-109	0.025824011032076793	4.6683291105332504E-4	5.187032345036946E-4	8.188101058951179E-4	6.669041586476073E-4	3.705023103597818E-5
110-114	0.025909226563459543	4.927680727785098E-4	5.187032345036946E-4	8.706804293454873E-4	6.669041586476073E-4	3.705023103597818E-5
115-119	0.025949981817599117	5.001781189857055E-4	5.298183038144881E-4	9.225507527958566E-4	6.743142048548029E-4	3.705023103597818E-5
120-124	0.02610188776484663	5.187032345036946E-4	5.483434193324771E-4	9.262557758994546E-4	7.2988955140877E-4	3.705023103597818E-5
125-129	0.026446454913481226	5.335233269180859E-4	5.557534655396728E-4	9.262557758994546E-4	7.595297362375527E-4	3.705023103597818E-5
130-134	0.026739151738665458	5.372283500216837E-4	5.742785810576618E-4	9.484859145210415E-4	8.002849903771287E-4	3.705023103597818E-5
135-139	0.027024438517642486	5.483434193324771E-4	6.1132881209364E-4	0.0010151763303858023	8.447452676203025E-4	3.705023103597818E-5
140-144	0.027394940828002267	5.557534655396728E-4	6.1132881209364E-4	0.0010670466538361717	8.706804293454873E-4	3.705023103597818E-5
145-149	0.027691342676290094	5.816886272648574E-4	6.557890893368137E-4	0.0010966868386649543	8.706804293454873E-4	3.705023103597818E-5
150-154	0.028009974663199506	5.928036965756509E-4	6.854292741655964E-4	0.0011485571621153237	8.780904755526829E-4	3.705023103597818E-5
155-159	0.028213750933897386	5.928036965756509E-4	7.261845283051722E-4	0.001204132508669291	8.892055448634764E-4	4.446027724317382E-5
160-164	0.028458282458734847	6.187388583008356E-4	7.410046207195636E-4	0.0012263626472908777	9.114356834850634E-4	5.5575346553967275E-5
165-167	0.028800379591967042	6.298539276116291E-4	7.410046207195637E-4	0.0012473577782112655	9.324308144054509E-4	6.175038505996363E-5
>>END_MODULE
