##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293800
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7476925
Total Bases	1.2 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.3517741584943	39.0	39.0	39.0	37.0	39.0
2	40.12619599634877	41.0	40.0	41.0	38.0	41.0
3	40.16350518428364	41.0	40.0	41.0	38.0	41.0
4	40.176199841512386	41.0	40.0	41.0	38.0	41.0
5	40.179648050502045	41.0	40.0	41.0	38.0	41.0
6	40.168730861951936	41.0	40.0	41.0	38.0	41.0
7	40.18995736883812	41.0	40.0	41.0	38.0	41.0
8	40.17113586133337	41.0	40.0	41.0	38.0	41.0
9	40.155475278941545	41.0	40.0	41.0	38.0	41.0
10-14	40.09705939273164	41.0	40.0	41.0	38.0	41.0
15-19	39.979832457862024	41.0	40.0	41.0	38.0	41.0
20-24	39.81051287795451	41.0	40.0	41.0	37.6	41.0
25-29	39.57555390752215	40.6	39.2	41.0	36.6	41.0
30-34	39.356540369202584	40.0	39.0	41.0	35.6	41.0
35-39	39.196120945442146	40.0	38.2	41.0	35.0	41.0
40-44	38.69394081122921	39.8	36.8	41.0	35.0	41.0
45-49	37.96598435854312	38.8	35.2	41.0	35.0	41.0
50-54	37.17570691159801	36.8	35.0	39.6	34.2	41.0
55-59	36.41625563985195	35.6	35.0	38.4	34.0	40.6
60-64	35.643483865359094	35.0	34.8	36.8	33.6	39.2
65-69	35.32169331643691	35.0	35.0	36.0	33.6	38.0
70-74	35.17631371185347	35.0	35.0	35.4	34.0	37.0
75-79	35.07027723295339	35.0	35.0	35.0	34.0	36.4
80-84	35.03272832080033	35.0	35.0	35.0	33.0	36.0
85-89	34.863304928162314	35.0	34.6	35.0	32.6	36.4
90-94	39.64380364477243	40.6	39.8	40.6	37.8	40.6
95-99	39.938140742300114	41.0	40.0	41.0	38.0	41.0
100-104	39.82221789777087	41.0	40.0	41.0	37.2	41.0
105-109	39.67735380243522	41.0	39.8	41.0	36.6	41.0
110-114	39.4975206846251	41.0	39.0	41.0	35.8	41.0
115-119	39.22184729765083	40.0	38.4	41.0	35.0	41.0
120-124	38.95541815189692	40.0	38.0	41.0	35.0	41.0
125-129	38.41157169480001	39.6	36.6	40.6	34.6	41.0
130-134	38.09160648368006	39.0	35.4	41.0	34.0	41.0
135-139	37.6524419294895	38.0	35.0	41.0	34.6	41.0
140-144	36.977149229252035	36.6	35.0	39.4	34.0	41.0
145-149	36.230675054508026	35.4	35.0	37.8	34.0	40.2
150-154	35.586301870250495	35.0	35.0	36.4	34.0	38.6
155-159	35.125004449512815	35.0	35.0	35.6	34.0	37.0
160-164	34.84166640280904	35.0	35.0	35.0	33.8	36.0
165-169	34.68039869638768	35.0	35.0	35.0	33.0	36.0
170-174	34.60349008219741	35.0	35.0	35.0	33.0	35.4
175-178	34.31878956756446	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	36.0
30	514.0
31	3435.0
32	17621.0
33	63966.0
34	173348.0
35	411055.0
36	1078494.0
37	2518731.0
38	2754253.0
39	453437.0
40	2033.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.256235016400456	26.91253423031527	27.936885818702205	22.894344934582065
2	23.15343540292299	27.812115274661707	27.523988805558435	21.510460516856863
3	21.238570669091906	26.6324190760239	28.87594833437543	23.253061920508767
4	21.798974845942684	26.065876011863164	29.36322084279299	22.771928299401157
5	22.867555846822054	26.402498353266886	27.523774813843925	23.206170986067136
6	23.027514653417015	26.638865576423466	27.19845390986268	23.135165860296848
7	22.864452966961686	27.11403417848915	27.088167929997958	22.933344924551204
8	23.01262885477653	26.980730714832635	26.858849058938	23.147791371452836
9	23.228412749893838	27.079086656613516	26.675953015444183	23.016547578048463
10-14	23.053953329744516	27.139229027976075	26.764821099583052	23.041996542696364
15-19	23.013474122048837	27.16149219097423	26.859515108149406	22.965518578827528
20-24	22.953864055076117	26.980904583100674	26.972374338381083	23.09285702344212
25-29	22.94626467431464	27.052976992547066	26.858084038558633	23.142674294579656
30-34	22.974286889329505	27.090136653771435	26.882944525991636	23.052631930907424
35-39	22.99199202880863	27.03342082473744	26.85195050104154	23.122636645412385
40-44	22.94061261815519	27.085003527519667	26.922578466415004	23.051805387910136
45-49	23.113127388598922	26.9962290647559	26.80124248939236	23.089401057252815
50-54	23.07501011445213	27.026078234033374	26.829377050057346	23.06953460145715
55-59	23.020937083092313	27.00433667583933	26.844623959716056	23.130102281352293
60-64	23.01547761947592	27.070414642383067	26.876532799245677	23.037574938895336
65-69	23.024352925835153	26.990301494264017	26.85576757824908	23.129578001651748
70-74	22.95301878780381	27.05459530488804	26.899026003336935	23.093359903971216
75-79	22.971036890165408	27.032826997729682	26.8993897892516	23.096746322853313
80-84	22.97388297996837	27.023304366434065	26.866293295706456	23.13651935789111
85-89	22.942915703982585	27.018561775061272	26.874443705132794	23.16407881582335
90-94	22.30922726304274	26.82244461811209	28.126402852838854	22.741925266006312
95-99	22.964871136560987	27.05234250216767	26.98697164391415	22.995814717357195
100-104	23.049576445555335	27.107956503520853	26.801213080549303	23.04125397037451
105-109	22.91536454321953	27.148701946570718	26.978976287817897	22.956957222391853
110-114	22.95711836870216	26.997386697658953	27.00565776828554	23.039837165353347
115-119	22.98558437346701	27.028719813313916	26.88172057563545	23.103975237583622
120-124	22.914514317688354	27.04874202260074	26.96206237178822	23.074681287922687
125-129	22.911213680982044	27.073900359286423	26.955369727345413	23.05951623238612
130-134	23.009655501016127	27.047147293965118	26.88060936347036	23.062587841548396
135-139	23.05052323210193	27.01679858517767	26.852556589513547	23.08012159320685
140-144	23.047946631254607	27.07736759856526	26.915157698267556	22.95952807191258
145-149	23.0278963092727	27.03493171064335	26.901131379295574	23.036040600788375
150-154	22.927803676232422	27.14452392755453	26.982505392503896	22.945167003709148
155-159	22.947021817862634	27.137933127465136	26.966474066596284	22.948570988075943
160-164	22.90447553712425	27.20915023019326	26.984372741933882	22.902001490748614
165-169	22.816519567970957	27.205802635962268	27.072063304160064	22.905614491906707
170-174	22.8229041411317	27.311084119975533	27.068241956793	22.79776978209977
175-178	22.810025537815314	27.296321407471456	27.025570395020765	22.868082659692465
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	3.0
15	6.0
16	14.0
17	34.0
18	76.0
19	156.0
20	325.0
21	689.5
22	1341.0
23	2432.0
24	4252.0
25	7160.5
26	11320.0
27	17219.0
28	25189.5
29	35390.5
30	48243.5
31	64019.0
32	83142.5
33	105360.0
34	130061.0
35	156487.5
36	184181.0
37	213006.0
38	242624.0
39	270902.0
40	297394.5
41	320663.0
42	339592.0
43	353949.5
44	361599.5
45	362596.0
46	357530.0
47	346174.5
48	329488.0
49	309164.5
50	286019.0
51	262290.0
52	238027.0
53	213864.5
54	191631.0
55	170508.5
56	151828.0
57	135196.5
58	119977.0
59	106327.5
60	93975.5
61	83670.5
62	74252.0
63	65417.5
64	57128.5
65	49447.5
66	42233.5
67	35275.0
68	28984.5
69	23470.5
70	18432.5
71	14106.0
72	10624.5
73	7735.0
74	5452.0
75	3706.0
76	2434.5
77	1540.0
78	939.5
79	561.5
80	331.5
81	179.0
82	85.0
83	42.5
84	23.0
85	7.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	827.0
92-93	1870.0
94-95	2938.0
96-97	4184.0
98-99	4583.0
100-101	5435.0
102-103	6111.0
104-105	7003.0
106-107	8102.0
108-109	9419.0
110-111	10334.0
112-113	9951.0
114-115	11451.0
116-117	12047.0
118-119	11946.0
120-121	11755.0
122-123	12362.0
124-125	15522.0
126-127	13634.0
128-129	13938.0
130-131	14407.0
132-133	16776.0
134-135	72738.0
136-137	76666.0
138-139	68476.0
140-141	65949.0
142-143	63617.0
144-145	67614.0
146-147	69675.0
148-149	74951.0
150-151	73856.0
152-153	84922.0
154-155	65083.0
156-157	65345.0
158-159	90147.0
160-161	68670.0
162-163	68844.0
164-165	72635.0
166-167	74144.0
168-169	78849.0
170-171	75127.0
172-173	82025.0
174-175	84247.0
176-177	228279.0
178-179	5500471.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.1162642818886
#Duplication Level	Percentage of total
1	90.83396661056945
2	7.712578990742627
3	1.014737553098027
4	0.22123356317719317
5	0.07389626112881453
6	0.036161806838665066
7	0.02808221574605393
8	0.019892994370578197
9	0.022132895191122208
>10	0.03731710913747241
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3374482156768993E-5	0.0	2.6748964313537986E-5	0.0	0.0	0.0
2	1.738682680379969E-4	2.6748964313537986E-5	2.6748964313537986E-5	1.3374482156768993E-5	0.0	0.0
3	1.872427501947659E-4	5.349792862707597E-5	2.6748964313537986E-5	1.3374482156768993E-5	0.0	0.0
4	2.006172323515349E-4	6.687241078384496E-5	2.6748964313537986E-5	2.6748964313537986E-5	0.0	0.0
5	2.006172323515349E-4	6.687241078384496E-5	5.349792862707597E-5	2.6748964313537986E-5	1.3374482156768993E-5	0.0
6	2.006172323515349E-4	6.687241078384496E-5	6.687241078384496E-5	2.6748964313537986E-5	1.3374482156768993E-5	0.0
7	2.139917145083039E-4	6.687241078384496E-5	6.687241078384496E-5	2.6748964313537986E-5	1.3374482156768993E-5	0.0
8	2.139917145083039E-4	6.687241078384496E-5	6.687241078384496E-5	4.0123446470306975E-5	1.3374482156768993E-5	0.0
9	2.139917145083039E-4	8.024689294061395E-5	6.687241078384496E-5	4.0123446470306975E-5	1.3374482156768993E-5	0.0
10-14	2.139917145083039E-4	1.0699585725415194E-4	7.489710007790635E-5	6.152261792113735E-5	1.3374482156768993E-5	0.0
15-19	2.4609047168454946E-4	1.0967075368550575E-4	8.292178937196775E-5	1.3106992513633612E-4	1.3374482156768993E-5	0.0
20-24	2.8621391815485646E-4	1.2572013227362853E-4	9.362137509738295E-5	1.3374482156768992E-4	2.139917145083039E-5	0.0
25-29	2.942386074489178E-4	1.604937858812279E-4	1.0967075368550573E-4	1.604937858812279E-4	2.6748964313537986E-5	0.0
30-34	3.1296288246839444E-4	1.899176466261197E-4	1.471193037244589E-4	2.2736619666507288E-4	2.6748964313537986E-5	0.0
35-39	4.440328076047305E-4	2.059670252142425E-4	2.086419216455963E-4	2.7551433242944127E-4	3.477365360759938E-5	8.024689294061397E-6
40-44	5.724278363097129E-4	2.4074067882184184E-4	2.3806578239048804E-4	3.18312675331102E-4	6.152261792113737E-5	1.3374482156768993E-5
45-49	7.195471400341718E-4	2.9156371101756405E-4	2.594649538413185E-4	3.7448550038953176E-4	9.629627152873675E-5	2.139917145083039E-5
50-54	8.559668580332157E-4	3.343620539192248E-4	2.8353902172350263E-4	4.279834290166078E-4	1.1502054654821334E-4	3.477365360759938E-5
55-59	0.001048559401090689	3.744855003895318E-4	3.2633736462516345E-4	4.761315647809761E-4	1.604937858812279E-4	5.884772148978357E-5
60-64	0.0011368309833253644	3.798352932522394E-4	3.557612253700552E-4	4.895060469377452E-4	1.9794233592018107E-4	9.362137509738295E-5
65-69	0.0012545264263049316	3.9855956827171597E-4	3.9855956827171597E-4	5.216048041139907E-4	2.8086412529214886E-4	9.362137509738295E-5
70-74	0.0020810694235932556	4.0123446470306985E-4	4.1995873972254635E-4	5.804525256037742E-4	3.183126753311021E-4	9.897116796009054E-5
75-79	0.017223658121487108	4.1995873972254635E-4	4.681068754869147E-4	6.045265934859585E-4	3.3703695035057864E-4	1.2304523584227473E-4
80-84	0.023953697542773267	4.5473239333014575E-4	5.002056326631603E-4	6.473249363876194E-4	4.1995873972254635E-4	1.3374482156768992E-4
85-89	0.02404196912500794	4.8415625407503756E-4	5.216048041139907E-4	6.954730721519877E-4	5.670780434470053E-4	1.3374482156768992E-4
90-94	0.024151639878693447	5.109052183885755E-4	5.403290791334673E-4	7.195471400341718E-4	5.884772148978356E-4	1.3374482156768992E-4
95-99	0.024226536978771352	5.349792862707597E-4	5.670780434470053E-4	7.436212079163561E-4	5.884772148978356E-4	1.3374482156768992E-4
100-104	0.02426666042524166	5.483537684275287E-4	5.804525256037742E-4	7.596705865044788E-4	5.938270077605432E-4	1.3374482156768992E-4
105-109	0.024320158353868738	5.617282505842977E-4	6.09876386348666E-4	8.024689294061395E-4	6.045265934859585E-4	1.3374482156768992E-4
110-114	0.024411104832534766	5.831274220351281E-4	6.606994185443882E-4	8.532919616018617E-4	6.152261792113737E-4	1.3374482156768992E-4
115-119	0.02450472620763215	6.152261792113737E-4	6.820985899952186E-4	9.201643723857067E-4	6.23250868505435E-4	1.3374482156768992E-4
120-124	0.02459032289343547	6.152261792113737E-4	6.954730721519876E-4	9.495882331305985E-4	6.36625350662204E-4	1.3641971799904372E-4
125-129	0.02478291543649294	6.419751435249116E-4	7.195471400341718E-4	9.763371974441364E-4	6.633743149757421E-4	1.471193037244589E-4
130-134	0.024986207565275833	6.580245221130346E-4	7.409463114850022E-4	9.97736368894967E-4	6.820985899952186E-4	1.471193037244589E-4
135-139	0.025333944101351823	6.76748797132511E-4	7.730450686612478E-4	0.0010592589868161043	6.954730721519876E-4	1.471193037244589E-4
140-144	0.025644232087388863	6.820985899952186E-4	8.265429972883239E-4	0.0010779832618355808	7.088475543087566E-4	1.471193037244589E-4
145-149	0.025919746419818313	6.820985899952186E-4	8.613166508959233E-4	0.001118106708305888	7.275718293282332E-4	1.6584357874393547E-4
150-154	0.02617386158079692	6.820985899952186E-4	8.827158223467535E-4	0.0011475305690507795	7.355965186222946E-4	1.872427501947659E-4
155-159	0.026382503502442516	6.927981757206339E-4	8.827158223467535E-4	0.001176954429795671	7.436212079163561E-4	1.872427501947659E-4
160-164	0.02664731824914654	7.008228650146952E-4	8.987652009348763E-4	0.0011983536012465017	7.783948615239553E-4	1.872427501947659E-4
165-167	0.026860418331511055	7.088475543087566E-4	9.228392688170605E-4	0.0012170778762659784	8.024689294061396E-4	1.872427501947659E-4
>>END_MODULE
