##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293803
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8501031
Total Bases	1.3 Gbp
Sequences flagged as poor quality	0
Sequence length	90-169
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.01125875202666	41.0	40.0	41.0	38.0	41.0
2	40.1055947213932	41.0	40.0	41.0	38.0	41.0
3	40.16274567167206	41.0	40.0	41.0	38.0	41.0
4	40.1663967582285	41.0	40.0	41.0	38.0	41.0
5	40.1493097719559	41.0	40.0	41.0	38.0	41.0
6	40.12758193682625	41.0	40.0	41.0	38.0	41.0
7	40.115593979130296	41.0	40.0	41.0	38.0	41.0
8	40.10131159385256	41.0	40.0	41.0	38.0	41.0
9	40.07750471678082	41.0	40.0	41.0	38.0	41.0
10-14	39.98770008014323	41.0	40.0	41.0	38.0	41.0
15-19	39.80605679475819	41.0	40.0	41.0	37.6	41.0
20-24	39.54540993910033	40.0	39.0	41.0	36.6	41.0
25-29	39.28166056564198	40.0	38.6	41.0	35.2	41.0
30-34	38.94101774243618	40.0	37.6	41.0	35.0	41.0
35-39	38.35372309546925	39.6	36.4	41.0	35.0	41.0
40-44	37.83344982508592	38.6	35.0	40.8	34.8	41.0
45-49	37.111237425201715	36.8	35.0	39.8	34.0	41.0
50-54	36.436964857556696	35.6	35.0	38.8	34.0	40.8
55-59	35.840747175254386	35.0	35.0	37.0	33.6	39.4
60-64	35.02206857027106	34.8	34.4	35.8	32.8	38.6
65-69	35.22676760030636	35.0	35.0	35.6	34.0	37.0
70-74	35.06798751821985	35.0	35.0	35.0	33.0	36.6
75-79	34.99324084337535	35.0	35.0	35.0	33.0	36.0
80-84	34.965426617077384	35.0	35.0	35.0	33.0	36.0
85-89	38.845147747373225	39.8	38.8	39.8	36.6	40.0
90-94	39.89559831549565	41.0	40.0	41.0	37.8	41.0
95-99	39.73597412909682	41.0	40.0	41.0	37.0	41.0
100-104	39.53514335814675	40.8	39.0	41.0	35.8	41.0
105-109	39.24536967265151	40.0	38.4	41.0	35.0	41.0
110-114	38.88731830771967	40.0	38.0	41.0	35.0	41.0
115-119	38.335790580258916	39.6	36.6	40.6	34.6	40.8
120-124	37.912362937460635	39.0	35.0	40.6	34.4	40.8
125-129	37.49760042082859	37.8	35.0	40.6	34.0	41.0
130-134	36.75909762348266	36.4	35.0	39.4	33.8	41.0
135-139	36.135256472773456	35.2	35.0	38.0	33.4	40.4
140-144	35.71167061886077	35.0	35.0	36.6	34.0	39.0
145-149	35.23819237127051	35.0	35.0	36.0	34.0	37.2
150-154	34.9118151807689	35.0	35.0	35.0	33.6	36.2
155-159	34.71438098837651	35.0	35.0	35.0	33.0	36.0
160-164	34.606570539455376	35.0	35.0	35.0	33.0	35.8
165-169	34.39007256327521	35.0	34.6	35.0	32.6	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	7.0
30	212.0
31	2441.0
32	15898.0
33	67705.0
34	215941.0
35	637312.0
36	1770125.0
37	2960079.0
38	2396793.0
39	432283.0
40	2233.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.317795218015323	26.078048650804824	27.344106850098537	23.26004928108132
2	23.04064059994605	26.26249686655654	27.33720180528691	23.359660728210496
3	23.071719183237892	26.592868559119477	26.96029458073968	23.375117676902953
4	23.312290003412528	26.822934771088352	26.63235788694336	23.23241733855576
5	23.386422188085188	26.833545248805702	26.628805376665486	23.15122718644362
6	23.01003254781685	26.914194290080818	26.718794461518847	23.356978700583493
7	23.28568146616569	26.924910637309758	26.61980646818015	23.169601428344397
8	23.54312082852068	26.80676026237288	26.575635355288078	23.074483553818354
9	23.14619250300346	26.796785001725087	26.510184470565985	23.546838024705472
10-14	23.27819531536822	26.819523420159275	26.671301398618592	23.230979865853918
15-19	23.14557610718041	26.678302902318553	26.84527088537849	23.330850105122543
20-24	23.205681757895015	26.630252259990584	26.72491136663306	23.439154615481346
25-29	23.256710862482443	26.741532879953034	26.612452066108215	23.389304191456304
30-34	23.182221074126186	26.684295116674672	26.649235839746964	23.484247969452177
35-39	23.206620467564466	26.709390896233643	26.66070974214775	23.423278894054146
40-44	23.349386680274428	26.652955388587575	26.542142947131943	23.455514984006058
45-49	23.41883002191146	26.624424731541385	26.494005256538884	23.46273999000827
50-54	23.436046757152162	26.58753273573523	26.520046803734747	23.45637370337786
55-59	23.407196138915385	26.65377646546637	26.48847416272215	23.450553232896105
60-64	23.33244050045224	26.61795022274357	26.556687065368894	23.492922211435296
65-69	23.366234048552464	26.6257680980107	26.534807366306513	23.473190487130328
70-74	23.33963492192888	26.662380127775087	26.530109112647633	23.467875837648396
75-79	23.296515446185293	26.637630188620655	26.52462036663553	23.54123399855853
80-84	23.298430508017205	26.638364217234354	26.548464533301903	23.514740741446538
85-89	23.21185983206037	26.68902160220331	26.753333801511843	23.34578476422448
90-94	23.18125212975471	26.875984899411097	26.633762620660807	23.309000350173385
95-99	23.196448649526786	26.833088299316294	26.71572498344348	23.254738067713443
100-104	23.127062433612654	26.711497698571936	26.83177623037885	23.329663637436564
105-109	23.162818726335534	26.68684791178051	26.66777445302496	23.482558908858994
110-114	23.193173948899585	26.725012279188288	26.661014251455743	23.420799520456388
115-119	23.18261081595167	26.737961913365105	26.657544675799667	23.421882594883563
120-124	23.138048498081414	26.757111236998377	26.674095498673744	23.430744766246463
125-129	23.238658352184547	26.69541828158365	26.58928332030921	23.476640045922597
130-134	23.380041974668433	26.71535744512228	26.52767490668219	23.376925673527097
135-139	23.26398798765117	26.771082553826574	26.56095486451878	23.403974594003476
140-144	23.2274591950726	26.831559506255033	26.66779063438951	23.273190664282858
145-149	23.170619706568704	26.8961170932542	26.71363599502008	23.219627205157018
150-154	23.08841259846379	26.961493471849696	26.754112923446716	23.195981006239798
155-159	23.04766722013187	26.97892055527441	26.83186518924173	23.141547035351994
160-164	22.989682908131893	27.119790394438564	26.84241476882203	23.04811192860751
165-169	22.886932418571842	27.173719623081773	26.906622838710458	23.032725119635927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.5
9	2.5
10	5.5
11	17.0
12	44.0
13	85.0
14	166.5
15	319.5
16	589.5
17	1041.5
18	1787.0
19	3089.5
20	4989.5
21	7603.0
22	11316.0
23	16171.5
24	22285.5
25	29857.5
26	38929.5
27	49395.5
28	61364.0
29	74777.0
30	89276.0
31	105295.0
32	122155.5
33	139682.5
34	158295.5
35	176835.5
36	195618.0
37	215076.5
38	233935.5
39	252654.5
40	270447.5
41	285744.0
42	299546.0
43	311269.0
44	319571.0
45	324482.5
46	326153.0
47	324317.5
48	318444.0
49	308970.0
50	296730.5
51	282189.0
52	266192.0
53	249754.5
54	233765.0
55	218832.0
56	204624.5
57	190821.0
58	178585.5
59	167672.5
60	156661.0
61	145453.0
62	133066.0
63	120355.0
64	107202.0
65	93057.5
66	79602.0
67	66450.5
68	54156.5
69	43001.0
70	33046.5
71	24738.5
72	18027.5
73	12735.0
74	8699.0
75	5827.0
76	3736.5
77	2307.5
78	1383.5
79	787.5
80	415.5
81	231.0
82	127.0
83	55.5
84	23.5
85	11.5
86	4.5
87	3.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1083.0
92-93	3430.0
94-95	4000.0
96-97	5329.0
98-99	6463.0
100-101	7394.0
102-103	8764.0
104-105	9056.0
106-107	9845.0
108-109	10549.0
110-111	10907.0
112-113	11618.0
114-115	11878.0
116-117	12781.0
118-119	13456.0
120-121	14440.0
122-123	14987.0
124-125	15349.0
126-127	16149.0
128-129	32811.0
130-131	100346.0
132-133	112074.0
134-135	103849.0
136-137	96146.0
138-139	85164.0
140-141	85978.0
142-143	94054.0
144-145	81635.0
146-147	78921.0
148-149	78617.0
150-151	85369.0
152-153	81684.0
154-155	83744.0
156-157	86662.0
158-159	91164.0
160-161	91884.0
162-163	95682.0
164-165	99107.0
166-167	116835.0
168-169	6531827.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.08357970534038
#Duplication Level	Percentage of total
1	94.34014823137915
2	5.197703123557457
3	0.3793119050044621
4	0.048838422800019765
5	0.019445723245356376
6	0.008235794719531042
7	0.0010355128033745931
8	0.0010415745042412481
9	0.0010476594376513557
>10	0.003192052548763224
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.176327906579802E-5	0.0	0.0	0.0	0.0
2	0.0	1.176327906579802E-5	0.0	2.352655813159604E-5	1.176327906579802E-5	0.0
3	0.0	1.176327906579802E-5	0.0	3.528983719739406E-5	1.176327906579802E-5	0.0
4	0.0	2.352655813159604E-5	0.0	4.705311626319208E-5	1.176327906579802E-5	0.0
5	0.0	4.705311626319208E-5	1.176327906579802E-5	5.88163953289901E-5	1.176327906579802E-5	0.0
6	1.176327906579802E-5	5.88163953289901E-5	1.176327906579802E-5	8.234295346058614E-5	1.176327906579802E-5	0.0
7	1.176327906579802E-5	5.88163953289901E-5	1.176327906579802E-5	8.234295346058614E-5	1.176327906579802E-5	0.0
8	1.176327906579802E-5	5.88163953289901E-5	1.176327906579802E-5	9.410623252638416E-5	3.528983719739406E-5	0.0
9	1.176327906579802E-5	7.057967439478812E-5	1.176327906579802E-5	1.176327906579802E-4	3.528983719739406E-5	0.0
10-14	1.4115934878957624E-5	7.057967439478812E-5	1.6468590692117228E-5	1.5998059529485304E-4	5.175842788951129E-5	0.0
15-19	2.5879213944755647E-5	7.057967439478812E-5	3.528983719739406E-5	1.9762308830540673E-4	7.057967439478812E-5	0.0
20-24	5.4111083702670884E-5	8.704826508690535E-5	4.940577207635168E-5	2.399708929422796E-4	9.410623252638416E-5	0.0
25-29	6.822701858162851E-5	1.270434139106186E-4	6.587436276846892E-5	2.7055541851335447E-4	1.176327906579802E-4	0.0
30-34	1.24690758097459E-4	1.341013813500974E-4	8.704826508690535E-5	2.9172932083179083E-4	1.293960697237782E-4	4.705311626319208E-6
35-39	2.8702400920547165E-4	1.6233325110801266E-4	1.199854464711398E-4	3.176085347765465E-4	1.740965301738107E-4	3.058452557107485E-5
40-44	4.7288381844508043E-4	2.1879699062384315E-4	1.293960697237782E-4	3.3172446965550414E-4	2.1409167899752394E-4	7.528498602110734E-5
45-49	5.575794277188262E-4	2.3291292550280075E-4	1.5762793948169348E-4	3.6466165103973857E-4	2.7290807432651406E-4	9.410623252638416E-5
50-54	6.281591021136141E-4	2.61144795260716E-4	1.6468590692117228E-4	4.234780463687287E-4	3.105505673370677E-4	1.1292747903166099E-4
55-59	6.940334648820832E-4	3.011399440844293E-4	1.7644918598697028E-4	4.7523647425824E-4	3.7407227429237704E-4	1.176327906579802E-4
60-64	7.575551718373924E-4	3.223138464028657E-4	2.0468105574488553E-4	5.505214602793473E-4	4.258307021818883E-4	1.3880669297641662E-4
65-69	7.7167110671635E-4	3.4348774872130215E-4	2.540868278212372E-4	6.046325439820182E-4	4.681785068187612E-4	1.4115934878957624E-4
70-74	0.008860101792359068	3.787775859186962E-4	2.587921394475564E-4	6.23453790487295E-4	5.246422463345917E-4	1.5762793948169348E-4
75-79	0.015449890725019117	4.211253905555691E-4	2.587921394475564E-4	6.446276928057315E-4	6.11690511421497E-4	1.7644918598697028E-4
80-84	0.015520470399413908	4.5170991612664396E-4	2.940819766449505E-4	7.034440881347215E-4	8.516614043637766E-4	1.7644918598697028E-4
85-89	0.015555760236611298	4.681785068187612E-4	3.246665022160253E-4	7.504972043979136E-4	8.940092090006495E-4	1.7644918598697028E-4
90-94	0.015591050073808694	4.893524091371976E-4	3.599563394134194E-4	8.563667159900958E-4	9.151831113190859E-4	1.7644918598697028E-4
95-99	0.015626339911006086	5.034683440161553E-4	3.85835553358175E-4	9.057724880664475E-4	9.410623252638416E-4	1.7644918598697028E-4
100-104	0.015640455845885047	5.175842788951128E-4	4.046567998634519E-4	9.551782601427991E-4	9.410623252638416E-4	1.7644918598697028E-4
105-109	0.015656924436577162	5.269949021477512E-4	4.1171476730293065E-4	0.0010022313764059911	9.528256043296395E-4	1.7644918598697028E-4
110-114	0.01567339302726928	5.458161486530281E-4	4.1406742311609024E-4	0.0010257579345375873	9.904680973401932E-4	1.7644918598697028E-4
115-119	0.015739267390037746	5.716953625977837E-4	4.5406257193980355E-4	0.0010492844926691834	0.0010257579345375873	1.7644918598697028E-4
120-124	0.01590865860858524	6.069851997951778E-4	4.7523647425823997E-4	0.0010822216740534179	0.0010845743298665772	1.811544976132895E-4
125-129	0.01610628169689065	6.211011346741353E-4	4.893524091371976E-4	0.001098690264745535	0.001117511511250812	1.8821246505276831E-4
130-134	0.016301552129382897	6.281591021136142E-4	5.081736556424744E-4	0.001110453543811333	0.0011786805623929615	1.8821246505276831E-4
135-139	0.016470943347930388	6.799175300031254E-4	5.22289590521432E-4	0.0011175115112508117	0.0011975018088982384	1.905651208659279E-4
140-144	0.016548580989764656	7.175600230136792E-4	5.293475579609109E-4	0.0011410380693824078	0.0012445549251614303	2.0703371155804512E-4
145-149	0.016616808008346282	7.175600230136792E-4	5.293475579609109E-4	0.0012022071205245576	0.0013033713204904204	2.2114964643700274E-4
150-154	0.016729735487377946	7.48144548584754E-4	5.3405286958723E-4	0.0012304389902824727	0.0013292505344351762	2.2350230225016233E-4
155-158	0.016895009558252405	7.704947788097702E-4	5.440516567931584E-4	0.00124690758097459	0.001352777092566772	2.2644312201661188E-4
>>END_MODULE
