##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293804
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6577850
Total Bases	1 Gbp
Sequences flagged as poor quality	0
Sequence length	90-168
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	40.03077829381941	41.0	40.0	41.0	38.0	41.0
2	40.14052646381416	41.0	40.0	41.0	38.0	41.0
3	40.15229717916949	41.0	40.0	41.0	38.0	41.0
4	40.15378793982836	41.0	40.0	41.0	38.0	41.0
5	40.14068152967915	41.0	40.0	41.0	38.0	41.0
6	40.12342589143869	41.0	40.0	41.0	38.0	41.0
7	40.11479495579863	41.0	40.0	41.0	38.0	41.0
8	40.08389093700829	41.0	40.0	41.0	38.0	41.0
9	40.06058514560229	41.0	40.0	41.0	38.0	41.0
10-14	39.967788502322186	41.0	40.0	41.0	38.0	41.0
15-19	39.769939752350695	40.8	40.0	41.0	37.6	41.0
20-24	39.48507612669793	40.0	39.0	41.0	36.4	41.0
25-29	39.236459238200936	40.0	38.4	41.0	35.0	41.0
30-34	38.817637252293686	40.0	37.4	41.0	35.0	41.0
35-39	38.22807756333756	39.2	36.0	41.0	35.0	41.0
40-44	37.667147228957795	38.2	35.0	40.6	34.6	41.0
45-49	36.9191646510638	36.4	35.0	39.4	34.0	41.0
50-54	36.247849586110966	35.4	35.0	38.4	34.0	40.6
55-59	35.42148130468162	34.8	34.6	36.6	32.8	39.0
60-64	35.22258193786723	35.0	34.8	36.0	33.4	38.0
65-69	35.15571762810037	35.0	35.0	35.4	33.6	37.0
70-74	35.02238266302819	35.0	35.0	35.0	33.0	36.2
75-79	34.96127549275219	35.0	35.0	35.0	33.0	36.0
80-84	34.835904376049925	35.0	34.8	35.0	32.6	36.0
85-89	39.980152146978114	41.0	40.0	41.0	38.0	41.0
90-94	39.897445539743345	41.0	40.0	41.0	37.8	41.0
95-99	39.71712746123809	41.0	40.0	41.0	37.0	41.0
100-104	39.505714287230674	40.6	39.0	41.0	35.8	41.0
105-109	39.19946291366448	40.0	38.4	41.0	35.0	41.0
110-114	38.865910104609085	40.0	37.8	41.0	35.0	41.0
115-119	38.12558243274226	39.4	36.2	40.4	34.4	40.6
120-124	37.97051199305544	39.0	35.2	40.8	34.4	41.0
125-129	37.3519794569043	37.4	35.0	40.4	34.0	41.0
130-134	36.611036046571314	36.2	35.0	39.2	33.6	41.0
135-139	36.04496725471289	35.0	35.0	37.6	33.6	40.0
140-144	35.59935408127639	35.0	35.0	36.4	34.0	38.8
145-149	35.15672199575572	35.0	35.0	35.8	34.0	37.0
150-154	34.85294296243703	35.0	35.0	35.0	33.2	36.0
155-159	34.677958199713274	35.0	35.0	35.0	33.0	36.0
160-164	34.573191567780626	35.0	35.0	35.0	33.0	35.6
165-168	34.33646491586243	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	4.0
30	137.0
31	1958.0
32	12045.0
33	51443.0
34	167497.0
35	505095.0
36	1405343.0
37	2319131.0
38	1811671.0
39	302686.0
40	840.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.361539104722667	26.293804206541648	27.050814475854573	23.293842212881106
2	23.14499418503006	26.579353436153152	26.858000714519182	23.417651664297605
3	23.236331020014138	26.779859680594726	26.694451834566006	23.289357464825134
4	23.454837066822744	26.75206944518346	26.581512196234332	23.211581291759465
5	23.11613977211399	26.87011713553821	26.637746376095535	23.37599671625227
6	23.24847784610473	26.934575887257996	26.68110400814856	23.135842258488715
7	23.56794393304803	26.73391761745859	26.503431972453008	23.19470647704037
8	23.224640269997035	26.73963377091299	26.469150254262413	23.566575704827567
9	23.268864446589692	26.848848787977836	26.539887653260564	23.34239911217191
10-14	23.271485363758675	26.782179587555206	26.656409009022703	23.289926039663417
15-19	23.201672278936126	26.637001451842167	26.80713607029653	23.35419019892518
20-24	23.240241112217518	26.624273888884666	26.6227232302348	23.51276176866301
25-29	23.28489400031925	26.675442583822985	26.597106957440502	23.442556458417265
30-34	23.238745182696473	26.67940740515518	26.610661538344594	23.47118587380375
35-39	23.230122304400375	26.68167258298684	26.58804320560669	23.50016190700609
40-44	23.372369391214455	26.587425982653905	26.49814149000053	23.542063136131105
45-49	23.497315992307517	26.609834520398003	26.430772972931887	23.462076514362597
50-54	23.44521994268644	26.56587486792797	26.451235586095763	23.537669603289828
55-59	23.43885616120769	26.594866103666092	26.47155225491612	23.4947254802101
60-64	23.42642048693722	26.589782375700267	26.487420661766382	23.49637647559613
65-69	23.370371778012572	26.611807809542633	26.477350502063747	23.540469910381052
70-74	23.379940254034373	26.57702744817836	26.497496902483338	23.545535395303936
75-79	23.372986614167242	26.610074720463373	26.471041449713812	23.54589721565557
80-84	23.33268773231375	26.5832027182134	26.467602636119704	23.616506913353145
85-89	23.187357571242885	26.702028778400237	26.777494166026894	23.333119484329988
90-94	23.285203435837833	26.803984702889323	26.579138815741715	23.331673045531126
95-99	23.170821024877323	26.780081119678282	26.726173628695282	23.322924226749112
100-104	23.19735026911615	26.610807738822	26.77764038726132	23.414201604800525
105-109	23.254707202841292	26.682528054262395	26.588280041802392	23.47448470109392
110-114	23.211549157962583	26.695066624237047	26.59939449463402	23.493989723166354
115-119	23.21454845368439	26.68580445572168	26.625347466122772	23.474299624471158
120-124	23.205861189286185	26.714368824113343	26.631601940382755	23.448168046217717
125-129	23.293635151287667	26.646311844902947	26.506954239949387	23.553098763859996
130-134	23.40173226174435	26.669170353594218	26.500378877638163	23.428718507023262
135-139	23.323607735709267	26.771460732001735	26.508812786228965	23.396118746060036
140-144	23.22572204667218	26.83214183839307	26.627184101130254	23.3149520138045
145-149	23.20562578843504	26.835393344841258	26.671651197476702	23.287329669246997
150-154	23.151949000775428	26.952576561393194	26.726658421908184	23.1688160159232
155-159	23.053015726678755	26.97669151873825	26.76376649652893	23.20652625805407
160-164	23.014416594099245	27.065318369970836	26.83108866814455	23.08917636778537
165-168	22.941786316199664	27.14179171811699	26.849525482664248	23.066896483019093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	2.5
10	7.0
11	13.5
12	36.0
13	67.0
14	120.5
15	246.5
16	424.5
17	757.0
18	1330.0
19	2266.0
20	3626.0
21	5570.0
22	8495.0
23	12269.0
24	16854.5
25	22373.0
26	29175.0
27	37357.0
28	46564.5
29	56721.0
30	67772.5
31	79998.5
32	93202.5
33	106920.0
34	120801.0
35	135045.0
36	150009.5
37	165434.5
38	180351.5
39	194487.0
40	207718.0
41	220435.5
42	231513.0
43	240678.0
44	247610.0
45	251486.5
46	253223.5
47	251456.5
48	246652.0
49	239568.0
50	230057.0
51	219226.0
52	207223.5
53	194242.5
54	181898.5
55	170120.5
56	158997.0
57	148913.5
58	139791.0
59	130962.0
60	122441.0
61	113662.5
62	103977.5
63	94165.5
64	83769.0
65	72892.5
66	62248.5
67	51894.5
68	42176.5
69	33542.5
70	25797.5
71	19349.5
72	14108.5
73	9952.5
74	6889.5
75	4598.0
76	2927.0
77	1778.0
78	1024.5
79	571.0
80	323.0
81	165.0
82	81.5
83	45.5
84	21.5
85	7.5
86	1.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-168	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	833.0
92-93	1847.0
94-95	2951.0
96-97	3930.0
98-99	4704.0
100-101	5410.0
102-103	6282.0
104-105	6810.0
106-107	7490.0
108-109	7966.0
110-111	8322.0
112-113	8625.0
114-115	8985.0
116-117	9560.0
118-119	10009.0
120-121	10617.0
122-123	11142.0
124-125	11834.0
126-127	12343.0
128-129	43839.0
130-131	77837.0
132-133	78762.0
134-135	79818.0
136-137	67533.0
138-139	63507.0
140-141	67541.0
142-143	67147.0
144-145	60604.0
146-147	59863.0
148-149	61379.0
150-151	62059.0
152-153	64038.0
154-155	65745.0
156-157	67760.0
158-159	71173.0
160-161	72385.0
162-163	75421.0
164-165	78587.0
166-167	166172.0
168-169	4987020.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.68791402336659
#Duplication Level	Percentage of total
1	95.48546954797797
2	4.220566115385108
3	0.23649520931522702
4	0.042955318511205036
5	0.008244234805422163
6	0.004153407622561602
7	0.0
8	0.001054113622895998
9	0.0010620527596086607
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	1.52025357829686E-5	1.52025357829686E-5	0.0	0.0
2	0.0	0.0	1.52025357829686E-5	3.04050715659372E-5	0.0	0.0
3	0.0	3.04050715659372E-5	3.04050715659372E-5	3.04050715659372E-5	1.52025357829686E-5	0.0
4	0.0	3.04050715659372E-5	3.04050715659372E-5	4.56076073489058E-5	1.52025357829686E-5	0.0
5	0.0	4.56076073489058E-5	3.04050715659372E-5	7.6012678914843E-5	1.52025357829686E-5	0.0
6	0.0	4.56076073489058E-5	4.56076073489058E-5	9.12152146978116E-5	1.52025357829686E-5	0.0
7	0.0	4.56076073489058E-5	4.56076073489058E-5	9.12152146978116E-5	1.52025357829686E-5	0.0
8	0.0	4.56076073489058E-5	4.56076073489058E-5	1.0641775048078019E-4	3.04050715659372E-5	0.0
9	0.0	4.56076073489058E-5	4.56076073489058E-5	1.0641775048078019E-4	3.04050715659372E-5	0.0
10-14	3.952659303571835E-5	4.56076073489058E-5	4.8648114505499516E-5	1.3074180773352994E-4	3.04050715659372E-5	0.0
15-19	6.08101431318744E-5	4.56076073489058E-5	8.513420038462417E-5	1.976329651785918E-4	6.08101431318744E-5	0.0
20-24	8.513420038462417E-5	6.08101431318744E-5	1.0945825763737391E-4	2.4628107968409135E-4	7.6012678914843E-5	0.0
25-29	1.1553927195056133E-4	7.90531860714367E-5	1.398633292033111E-4	3.131722371291532E-4	9.729622901099903E-5	0.0
30-34	1.5202535782968602E-4	1.0641775048078018E-4	1.8851144370881064E-4	3.648608587912464E-4	1.246607934203425E-4	0.0
35-39	2.949291941895908E-4	1.1249876479396764E-4	2.1283550096156035E-4	4.195899876099334E-4	1.976329651785918E-4	9.12152146978116E-6
40-44	4.6215708780224544E-4	1.3682282204671738E-4	2.3715955821431014E-4	4.4999505917587055E-4	2.797266584066222E-4	3.648608587912464E-5
45-49	5.320887524039009E-4	1.5810637214287344E-4	2.584431083104662E-4	4.7127860927202654E-4	3.466178158516841E-4	6.993166460165555E-5
50-54	5.928988955357753E-4	1.7026840076924833E-4	2.584431083104662E-4	5.260077380907135E-4	3.9222542320058987E-4	8.209369322803044E-5
55-59	6.597900529808372E-4	1.824304293956232E-4	2.645241226236536E-4	6.050609241621503E-4	4.3175201623630823E-4	9.12152146978116E-5
60-64	6.932356317033682E-4	1.976329651785918E-4	3.010102085027783E-4	6.901951245467744E-4	5.138457094643387E-4	9.12152146978116E-5
65-69	0.0011371496765660513	2.3411905105771638E-4	3.2837477291212177E-4	7.449242533654614E-4	5.807368669094005E-4	1.0337724332418648E-4
70-74	0.010507992733187897	2.675646297802473E-4	3.466178158516841E-4	8.02693889340742E-4	6.780330959203995E-4	1.3074180773352994E-4
75-79	0.014570110294397105	2.858076727198097E-4	3.770228874176213E-4	8.452609895330543E-4	7.905318607143672E-4	1.52025357829686E-4
80-84	0.014579231815866886	3.1013172997255945E-4	3.8006339457421497E-4	9.121521469781159E-4	0.0010368129403984583	1.52025357829686E-4
85-89	0.014588353337336666	3.192532514423406E-4	3.9222542320058987E-4	9.790433044231778E-4	0.001076339533434177	1.52025357829686E-4
90-94	0.014618758408902607	3.405368015384966E-4	3.983064375137773E-4	0.0010337724332418648	0.0010793800405907706	1.52025357829686E-4
95-99	0.01465524449478173	3.5877984447805887E-4	4.3783303054949565E-4	0.0010550559833380209	0.0010793800405907706	1.52025357829686E-4
100-104	0.014673487537721292	3.770228874176213E-4	4.4087353770608944E-4	0.001064177504807802	0.0010793800405907706	1.52025357829686E-4
105-109	0.014722135652226789	4.013469446703711E-4	4.4087353770608944E-4	0.0011432306908792384	0.0010793800405907706	1.52025357829686E-4
110-114	0.014773824273888883	4.287115090797145E-4	4.4999505917587055E-4	0.0012770130057693622	0.0010793800405907706	1.52025357829686E-4
115-119	0.014865039488586695	4.439140448626831E-4	4.5607607348905797E-4	0.001325661120274862	0.0011006635906869267	1.5506586498627973E-4
120-124	0.015038348396512538	4.5911658064565176E-4	4.5607607348905797E-4	0.0013651877133105802	0.0011097851121567078	1.7634941508243572E-4
125-129	0.015239021868847725	4.7127860927202654E-4	4.7127860927202654E-4	0.0014138358278160798	0.0011584332266622072	1.824304293956232E-4
130-134	0.015406249762460378	4.8344063789840144E-4	4.7127860927202654E-4	0.0014928890138875164	0.001216202862637488	1.824304293956232E-4
135-139	0.015512667512941159	4.925621593681827E-4	4.7127860927202654E-4	0.0015354561140798284	0.001249648441360019	1.824304293956232E-4
140-144	0.015634287799204907	5.260077380907135E-4	5.016836808379638E-4	0.0016084282858380779	0.0012922155415523307	1.824304293956232E-4
145-149	0.015771110621251628	5.533723025000571E-4	5.229672309341198E-4	0.0017178865434754518	0.0013439041632144241	1.824304293956232E-4
150-154	0.015929216993394495	5.776963597528068E-4	5.32088752403901E-4	0.001769575165137545	0.0013773497419369552	1.824304293956232E-4
155-157	0.016079215346453124	5.928988955357753E-4	5.32088752403901E-4	0.0018091017581732633	0.0014138358278160798	1.8243042939562322E-4
>>END_MODULE
