##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293814
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8700361
Total Bases	1.4 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.35828846642111	39.0	39.0	39.0	37.0	39.0
2	40.068337739089216	41.0	40.0	41.0	38.0	41.0
3	40.1373716561876	41.0	40.0	41.0	38.0	41.0
4	40.17185103008944	41.0	40.0	41.0	38.0	41.0
5	40.18332859981327	41.0	40.0	41.0	38.0	41.0
6	40.17895039067919	41.0	40.0	41.0	38.0	41.0
7	40.199115760828775	41.0	40.0	41.0	38.0	41.0
8	40.18228818321447	41.0	40.0	41.0	38.0	41.0
9	40.16306587738141	41.0	40.0	41.0	38.0	41.0
10-14	40.10718231117077	41.0	40.0	41.0	38.0	41.0
15-19	39.97693992237793	41.0	40.0	41.0	38.0	41.0
20-24	39.79697203368918	41.0	40.0	41.0	37.6	41.0
25-29	39.54599709138507	40.0	39.0	41.0	36.6	41.0
30-34	39.32057152571026	40.0	38.8	41.0	35.6	41.0
35-39	38.944248727150516	40.0	37.6	41.0	35.0	41.0
40-44	38.380077907112124	39.4	36.4	41.0	35.0	41.0
45-49	37.82719344634091	38.6	35.0	40.8	34.8	41.0
50-54	37.05642060139804	36.8	35.0	39.6	34.0	41.0
55-59	36.29550541638445	35.6	35.0	38.4	34.0	40.4
60-64	35.408482682500185	34.8	34.6	36.6	33.2	38.8
65-69	35.18171804595234	35.0	34.8	36.0	33.4	37.4
70-74	35.11667948031122	35.0	35.0	35.2	34.0	36.8
75-79	35.00048639361056	35.0	35.0	35.0	33.0	36.0
80-84	34.94880180259187	35.0	35.0	35.0	33.0	36.0
85-89	34.83523228518909	35.0	34.8	35.0	32.6	36.0
90-94	39.58540669206741	40.6	39.8	40.6	37.8	40.6
95-99	39.89543335320862	41.0	40.0	41.0	38.0	41.0
100-104	39.78604324864871	41.0	40.0	41.0	37.0	41.0
105-109	39.62330511698238	41.0	39.4	41.0	36.4	41.0
110-114	39.433066664566375	40.4	39.0	41.0	35.6	41.0
115-119	39.13826337850105	40.0	38.2	41.0	35.0	41.0
120-124	38.83641604614889	40.0	37.8	41.0	35.0	41.0
125-129	38.126794604147165	39.4	36.4	40.4	34.4	40.6
130-134	37.99544245467776	39.0	35.4	40.8	34.0	41.0
135-139	37.3973644177006	37.6	35.0	40.4	34.0	41.0
140-144	36.634276020962574	36.4	35.0	39.2	33.6	41.0
145-149	35.9925906022958	35.0	35.0	37.6	33.6	39.6
150-154	35.505557765209744	35.0	35.0	36.4	34.0	38.4
155-159	35.05489998782816	35.0	35.0	35.6	33.8	36.8
160-164	34.7760080160812	35.0	35.0	35.0	33.0	36.0
165-169	34.626059127325355	35.0	35.0	35.0	33.0	36.0
170-174	34.541845205275834	35.0	35.0	35.0	33.0	35.0
175-178	34.31658508037396	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	8.0
30	176.0
31	2272.0
32	14979.0
33	62955.0
34	195580.0
35	528585.0
36	1470336.0
37	3142755.0
38	2938638.0
39	343253.0
40	824.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.68502421910999	26.900481485768236	27.80691513834886	22.60757915677292
2	23.064387788046954	27.712551237816452	27.673288499178366	21.549772474958225
3	20.643476747688975	26.47859094582397	29.87026630274307	23.00766600374398
4	21.72745475733708	25.308593516981652	30.007214643162506	22.95673708251876
5	22.885291771226505	26.06995272954766	27.8809005741256	23.163854925100235
6	22.741849447396493	26.482487335870314	27.73548132083255	23.04018189590064
7	22.975897207023937	26.651779161807195	27.122081486044085	23.250242145124783
8	23.15528056824309	26.776141817563666	26.822289327994554	23.2462882861987
9	23.12673002878846	26.89519434883219	26.899665427675934	23.078410194703416
10-14	23.222859373306466	26.954129834382734	26.635202838135108	23.187807954175693
15-19	23.123153165713468	26.9286918094548	26.860499236755807	23.08765578807592
20-24	23.055211157330138	26.760901070656722	26.982399925704232	23.201487846308904
25-29	23.108282518392052	26.748848697197737	26.78507937774076	23.357789406669447
30-34	23.094977323354744	26.827946564516115	26.822038763678886	23.25503734845025
35-39	23.047655149021978	26.846883709767905	26.851504207698966	23.253956933511148
40-44	23.142409837936608	26.802655659920315	26.72923112040983	23.32570338173324
45-49	23.27824787959948	26.722146356915534	26.710436497979796	23.28916926550519
50-54	23.2740503526233	26.787035618407096	26.700565643195723	23.238348385773875
55-59	23.253784526871932	26.74004676357682	26.673192066398165	23.332976643153085
60-64	23.20394981311695	26.79589731966294	26.732189618338825	23.267963248881284
65-69	23.17965197076305	26.791679103890058	26.74952912873385	23.279139796613038
70-74	23.167277771577524	26.784851800976995	26.69930822410702	23.34856220333846
75-79	23.14015935660601	26.779773850763206	26.768114564441635	23.311952228189153
80-84	23.14019843544423	26.81119323669443	26.719109701310096	23.329498626551242
85-89	23.126477165717606	26.752437054048677	26.71814192537528	23.402943854858435
90-94	22.245555440701935	26.587507485922053	28.425285255933925	22.741651817442087
95-99	22.96077390069679	26.83771937474949	27.005939646979048	23.19556707757467
100-104	23.148308499263315	27.01773769502743	26.694971581714388	23.138982223994866
105-109	23.013591522350318	26.9528109109259	26.8799526431657	23.153644923558083
110-114	23.007178311991407	26.78854989727375	26.9676073686111	23.23666442212374
115-119	23.058281045796768	26.876243711643493	26.781902814553032	23.283572428006703
120-124	23.05524718236124	26.884606334517873	26.747097987905455	23.313048495215433
125-129	23.000874740788362	26.880850266884625	26.872647155695965	23.245627836631048
130-134	23.11187256739421	26.896284715854556	26.742359099497133	23.249483617254104
135-139	23.211208548947415	26.8375138441724	26.66245243928201	23.288825167598176
140-144	23.157697735394166	26.927608472991665	26.723715358386723	23.190978433227443
145-149	23.102675779245267	26.9693482113264	26.748500430204608	23.17947557922373
150-154	23.0481496383855	27.014414359689155	26.792405073010134	23.145030928915215
155-159	23.00619337774947	27.023157931025548	26.879356324674404	23.09129236655058
160-164	22.985997288306148	27.116328942374324	26.842814646946977	23.05485912237255
165-169	22.92825974089352	27.118705624366545	26.87868238964297	23.074352245096964
170-174	22.8683533963988	27.19420405577245	26.94877750290214	22.988665044926616
175-178	22.825601140129788	27.244647532079192	26.921052227643962	23.00869910014706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	0.5
13	1.0
14	3.0
15	12.0
16	26.5
17	56.5
18	134.0
19	293.5
20	613.5
21	1084.0
22	1984.5
23	3509.0
24	5718.0
25	9111.0
26	13997.5
27	20695.0
28	29666.5
29	41316.0
30	56179.5
31	74597.0
32	96612.5
33	121435.0
34	149704.0
35	180910.5
36	213292.0
37	246690.5
38	279167.0
39	309695.5
40	338504.5
41	363343.0
42	382342.0
43	395789.5
44	402884.5
45	403235.0
46	397260.5
47	384916.0
48	368405.0
49	347808.5
50	324201.5
51	300548.0
52	276754.5
53	253352.0
54	230455.5
55	208359.5
56	187826.5
57	169522.0
58	153430.0
59	138329.0
60	124876.5
61	112676.0
62	100545.5
63	89070.5
64	78016.0
65	67169.5
66	56556.0
67	46792.0
68	37890.0
69	29685.5
70	22681.0
71	16773.5
72	11974.5
73	8286.5
74	5538.0
75	3593.5
76	2246.5
77	1297.0
78	727.0
79	414.0
80	239.0
81	122.0
82	57.5
83	32.5
84	18.0
85	9.0
86	2.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	1066.0
92-93	2293.0
94-95	3338.0
96-97	4512.0
98-99	5752.0
100-101	6613.0
102-103	7553.0
104-105	8546.0
106-107	9194.0
108-109	10170.0
110-111	10797.0
112-113	11253.0
114-115	11669.0
116-117	11932.0
118-119	12628.0
120-121	12690.0
122-123	13197.0
124-125	13886.0
126-127	14840.0
128-129	15365.0
130-131	16027.0
132-133	17650.0
134-135	120015.0
136-137	90228.0
138-139	90471.0
140-141	90411.0
142-143	92945.0
144-145	97124.0
146-147	82988.0
148-149	78625.0
150-151	77712.0
152-153	77853.0
154-155	71835.0
156-157	73777.0
158-159	74064.0
160-161	76909.0
162-163	79454.0
164-165	81048.0
166-167	83147.0
168-169	87962.0
170-171	89605.0
172-173	94414.0
174-175	105951.0
176-177	199128.0
178-179	6463724.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.50491293669016
#Duplication Level	Percentage of total
1	87.82084361410746
2	10.267663530755113
3	1.2865781295570378
4	0.2714039737244423
5	0.11348024071676148
6	0.0812291370053974
7	0.04239857231248631
8	0.034320933708404375
9	0.021965312098706738
>10	0.058423850819417276
>50	0.0
>100	0.0016927051947801183
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	1.1493775947917563E-5	0.0	0.0
2	3.448132784375269E-5	0.0	0.0	1.1493775947917563E-5	0.0	0.0
3	4.597510379167025E-5	0.0	1.1493775947917563E-5	3.448132784375269E-5	0.0	0.0
4	4.597510379167025E-5	0.0	2.2987551895835126E-5	4.597510379167025E-5	0.0	0.0
5	4.597510379167025E-5	0.0	2.2987551895835126E-5	4.597510379167025E-5	1.1493775947917563E-5	0.0
6	4.597510379167025E-5	0.0	2.2987551895835126E-5	5.746887973958782E-5	1.1493775947917563E-5	0.0
7	4.597510379167025E-5	0.0	3.448132784375269E-5	5.746887973958782E-5	1.1493775947917563E-5	0.0
8	5.746887973958782E-5	2.2987551895835126E-5	3.448132784375269E-5	5.746887973958782E-5	1.1493775947917563E-5	0.0
9	5.746887973958782E-5	2.2987551895835126E-5	4.597510379167025E-5	5.746887973958782E-5	1.1493775947917563E-5	0.0
10-14	6.206639011875484E-5	2.758506227500215E-5	7.585892125625592E-5	9.884647315209103E-5	2.2987551895835126E-5	0.0
15-19	1.0344398353125807E-4	3.448132784375269E-5	1.3792531137501077E-4	1.287302906166767E-4	2.2987551895835126E-5	0.0
20-24	1.3792531137501077E-4	6.206639011875484E-5	1.7930290478751398E-4	1.471203321333448E-4	2.528630708541864E-5	0.0
25-29	1.7240663921876345E-4	8.505394201458998E-5	2.1148547744168317E-4	1.609128632708459E-4	3.448132784375269E-5	2.2987551895835124E-6
30-34	2.0918672225209964E-4	1.1493775947917562E-4	2.505643156646029E-4	1.6780912883959645E-4	3.448132784375269E-5	2.0688796706251613E-5
35-39	3.034356850250237E-4	1.471203321333448E-4	2.758506227500215E-4	1.8849792554584803E-4	5.976763492917133E-5	3.67800830333362E-5
40-44	3.5860580957502796E-4	1.5401659770209535E-4	3.149294609729412E-4	2.413692949062688E-4	8.505394201458998E-5	5.5170124550004305E-5
45-49	4.252697100729498E-4	1.6321161846042943E-4	3.67800830333362E-4	2.6435684680210396E-4	1.0344398353125807E-4	6.206639011875484E-5
50-54	4.988298761396222E-4	1.7240663921876345E-4	3.9308713741878065E-4	2.6435684680210396E-4	1.1493775947917562E-4	6.896265568750538E-5
55-59	5.677925318271276E-4	1.83900415166681E-4	4.045809133666982E-4	2.666556019916875E-4	1.2183402504792618E-4	7.815767644583944E-5
60-64	5.746887973958781E-4	1.9309543592501503E-4	4.275684652625334E-4	3.034356850250237E-4	1.609128632708459E-4	1.103402491000086E-4
65-69	5.884813285333792E-4	2.2987551895835124E-4	4.804398346229541E-4	3.425145232479434E-4	2.0918672225209964E-4	1.5171784251251185E-4
70-74	8.896182583688194E-4	2.482655604750193E-4	5.172199176562903E-4	3.6550207514377854E-4	2.5286307085418643E-4	1.7470539440834695E-4
75-79	0.006287095443510906	2.73551867560438E-4	5.540000006896267E-4	4.022821581771147E-4	2.6435684680210396E-4	1.999917014937656E-4
80-84	0.008686995861436096	2.8044813312918854E-4	5.631950214479607E-4	4.275684652625334E-4	3.379170128687763E-4	2.183817430104337E-4
85-89	0.008707684658142345	3.333195024896094E-4	5.838838181542122E-4	4.873361001917046E-4	5.126224072771234E-4	2.183817430104337E-4
90-94	0.008723775944469431	3.5400829919586096E-4	6.436514530833836E-4	5.287136936042079E-4	5.172199176562903E-4	2.183817430104337E-4
95-99	0.008769751048261103	3.8159336147086307E-4	6.712365153583856E-4	5.654937766375441E-4	5.172199176562903E-4	2.183817430104337E-4
100-104	0.00883411619356944	3.999834029875312E-4	7.241078847188066E-4	6.183651459979649E-4	5.172199176562903E-4	2.183817430104337E-4
105-109	0.008891585073309028	4.2067219969378286E-4	7.769792540792273E-4	6.643402497896351E-4	5.172199176562903E-4	2.183817430104337E-4
110-114	0.008912273870015279	4.4595850677920144E-4	8.413443993875657E-4	6.781327809271362E-4	5.172199176562903E-4	2.2757676376876774E-4
115-119	0.008930663911531947	4.57452282727119E-4	8.827219928000688E-4	6.827302913063033E-4	5.172199176562903E-4	2.413692949062688E-4
120-124	0.008972041504944451	4.6204979310628607E-4	9.126058102646546E-4	7.103153535813054E-4	5.172199176562903E-4	2.4366805009585235E-4
125-129	0.009107668061129877	4.965311209500386E-4	9.470871381084073E-4	7.264066399083899E-4	5.172199176562903E-4	2.6435684680210396E-4
130-134	0.009227203330988221	5.172199176562903E-4	9.654771796250752E-4	7.493941918042251E-4	5.356099591729584E-4	2.6435684680210396E-4
135-139	0.00934443984565698	5.287136936042079E-4	9.976597522792442E-4	7.999668059750625E-4	5.746887973958781E-4	2.942406642666896E-4
140-144	0.009463975115515322	5.654937766375441E-4	0.0010229460593646632	8.390456441979821E-4	5.861825733437957E-4	2.9883817464585664E-4
145-149	0.009549029057529912	5.999751044812968E-4	0.001032141080122997	8.413443993875657E-4	6.045726148604638E-4	2.9883817464585664E-4
150-154	0.009617991713217417	6.091701252396309E-4	0.00106662240796675	8.505394201458996E-4	6.59742739410468E-4	2.9883817464585664E-4
155-159	0.009696149389663258	6.18365145997965E-4	0.0011309875532750883	8.620331960938173E-4	6.827302913063034E-4	2.9883817464585664E-4
160-164	0.009808788393952848	6.620414946000516E-4	0.00115167634998134	8.735269720417348E-4	7.126141087708889E-4	3.103319505937742E-4
165-167	0.009880816056559799	6.666390049792187E-4	0.001160871370739674	8.773582306910406E-4	7.241078847188064E-4	3.103319505937742E-4
>>END_MODULE
