##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293815
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3002395
Total Bases	514.3 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.379777810714444	39.0	39.0	39.0	37.0	39.0
2	40.11015172886979	41.0	40.0	41.0	38.0	41.0
3	40.15862170034256	41.0	40.0	41.0	38.0	41.0
4	40.18309449622718	41.0	40.0	41.0	38.0	41.0
5	40.190733730904824	41.0	40.0	41.0	38.0	41.0
6	40.182369408422275	41.0	40.0	41.0	38.0	41.0
7	40.202669868554935	41.0	40.0	41.0	38.0	41.0
8	40.18102814586355	41.0	40.0	41.0	38.0	41.0
9	40.15495862469795	41.0	40.0	41.0	38.0	41.0
10-14	40.09398523512063	41.0	40.0	41.0	38.0	41.0
15-19	39.95288741154978	41.0	40.0	41.0	38.0	41.0
20-24	39.76281741742842	40.8	40.0	41.0	37.6	41.0
25-29	39.50441011259344	40.0	39.0	41.0	36.6	41.0
30-34	39.29087558432518	40.0	38.8	41.0	35.6	41.0
35-39	38.92461604818819	40.0	37.8	41.0	35.0	41.0
40-44	38.38620441347657	39.4	36.4	41.0	35.0	41.0
45-49	37.87317744667174	38.8	35.2	40.8	34.8	41.0
50-54	37.12798589126347	36.8	35.0	39.6	34.0	41.0
55-59	36.37621665370479	35.6	35.0	38.4	34.0	40.6
60-64	35.43606654021206	34.8	34.6	36.6	32.8	39.0
65-69	35.2385981191682	35.0	34.8	36.0	33.4	38.2
70-74	35.17592535292658	35.0	35.0	35.4	34.0	37.0
75-79	35.04304576846151	35.0	35.0	35.0	33.0	36.4
80-84	34.98871707420243	35.0	35.0	35.0	33.0	36.0
85-89	34.861418367669806	35.0	34.8	35.0	32.6	36.2
90-94	39.590367328382776	40.6	39.8	40.6	37.8	40.6
95-99	39.895880919429736	41.0	40.0	41.0	38.0	41.0
100-104	39.775082639626724	41.0	40.0	41.0	37.0	41.0
105-109	39.60834299965807	41.0	39.4	41.0	36.4	41.0
110-114	39.40697944725199	40.4	39.0	41.0	35.4	41.0
115-119	39.11936470569534	40.0	38.0	41.0	35.0	41.0
120-124	38.80367188109936	40.0	37.8	41.0	35.0	41.0
125-129	38.04949659785716	39.4	36.2	40.4	34.4	40.6
130-134	37.967142344979294	39.0	35.4	40.8	34.0	41.0
135-139	37.38328283683778	37.6	35.0	40.4	34.0	41.0
140-144	36.601368433878996	36.2	35.0	39.2	33.8	41.0
145-149	35.95653496142404	35.0	35.0	37.2	33.6	39.6
150-154	35.4740295593783	35.0	35.0	36.2	34.0	38.2
155-159	35.02906764503375	35.0	35.0	35.4	33.6	36.8
160-164	34.76341847973506	35.0	35.0	35.0	33.0	36.0
165-169	34.611846528554466	35.0	35.0	35.0	33.0	36.0
170-174	34.529241707906344	35.0	35.0	35.0	33.0	35.0
175-178	34.287988269540406	35.0	34.5	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
29	2.0
30	63.0
31	641.0
32	4613.0
33	19992.0
34	64632.0
35	182067.0
36	514331.0
37	1093128.0
38	1008390.0
39	114334.0
40	202.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.67453149901995	26.948619352217147	27.741153312605437	22.635695836157467
2	23.056992834054146	27.72649834548752	27.708912384946018	21.507596435512315
3	20.691514607505006	26.490718243269125	29.847305234654335	22.970461914571533
4	21.68978432218279	25.3578226715672	29.983563122107515	22.968829884142494
5	22.826776623329042	26.17024075779503	27.886670474737667	23.116312144138263
6	22.76319405008335	26.517530171746223	27.72083619910105	22.998439579069377
7	22.942317716356442	26.647193323996344	27.14922586801537	23.261263091631847
8	23.17869567461976	26.77069472870825	26.827582646520526	23.223026950151464
9	23.105021158108777	26.934397372764074	26.92267339906974	23.0379080700574
10-14	23.204321883030048	26.96064974795122	26.663167238154873	23.17186113086386
15-19	23.104847963042836	26.93864065187958	26.893962986216003	23.062548398861576
20-24	23.02966132037923	26.779827437762187	27.007825419373532	23.18268582248505
25-29	23.0917317674723	26.772733101407376	26.79101184221263	23.34452328890769
30-34	23.07745649722971	26.833531230900665	26.817011086149556	23.272001185720068
35-39	23.032072728604998	26.86647826152122	26.84245743814521	23.258991571728572
40-44	23.144309792682176	26.819975386316592	26.736195603842933	23.299519217158302
45-49	23.30875850779128	26.718176655636583	26.704800667467136	23.268264169105
50-54	23.260077371565032	26.780640122302362	26.71779695876125	23.241485547371347
55-59	23.22330006544775	26.737001627034417	26.680500067446154	23.359198240071677
60-64	23.183578443209505	26.787487988755643	26.745954479673728	23.282979088361124
65-69	23.153582390058602	26.81994207957314	26.74538160368639	23.281093926681866
70-74	23.160297029538086	26.768449854199734	26.73815404035778	23.333099075904403
75-79	23.117491202856385	26.781805858323104	26.79544830044015	23.30525463838036
80-84	23.133991363561424	26.8058333430478	26.737621132462586	23.322554160928192
85-89	23.105680631629085	26.791531427410455	26.72026165777654	23.382526283183925
90-94	22.2446305824038	26.59372309045272	28.438207767937897	22.723438559205583
95-99	22.952766125683645	26.860948179616045	27.002967141394617	23.18331855330569
100-104	23.1439608763411	27.0267675887126	26.686940588391295	23.142330946555003
105-109	23.03564562057624	26.943092342599652	26.90865788800137	23.11260414882274
110-114	22.988814761312067	26.806570823901684	26.969851232509797	23.234763182276453
115-119	23.03909106678184	26.86472390092379	26.798353363482885	23.29783166881149
120-124	23.0285438716595	26.906285926511835	26.770903799037022	23.29426640279164
125-129	23.013243457724272	26.9084520463294	26.85059194963718	23.22771254630915
130-134	23.114215729629557	26.904862967691876	26.735664597388947	23.24525670528962
135-139	23.18038194228308	26.83440786955699	26.679213954015335	23.305996234144594
140-144	23.125408059783602	26.932375066083857	26.74101452714297	23.201202346989565
145-149	23.078371219408638	26.996854762715728	26.740209653481173	23.18456436439447
150-154	23.02504879754008	27.046078278591356	26.79778535873696	23.1310875651316
155-159	22.98408136734609	27.055163821140898	26.877387668476377	23.083367143036636
160-164	22.959963761024085	27.146963587658618	26.87160808857777	23.021464562739528
165-169	22.893419422208584	27.119987384926965	26.916465633143666	23.07012755972078
170-174	22.844629090765803	27.23679622632485	26.956975687979035	22.96159899493031
175-178	22.8214382321476	27.27805530564632	26.942987389288238	22.957519072917844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	7.0
16	9.5
17	26.0
18	51.0
19	99.5
20	180.0
21	351.5
22	687.0
23	1185.5
24	1946.0
25	3109.5
26	4769.0
27	7132.0
28	10273.0
29	14167.0
30	19234.5
31	25710.5
32	33114.5
33	41676.0
34	51599.0
35	62249.0
36	73882.5
37	85615.0
38	96748.0
39	107411.5
40	117405.0
41	125979.5
42	132183.5
43	136735.5
44	139713.5
45	139629.0
46	137024.0
47	132797.5
48	127172.5
49	120185.0
50	112157.5
51	103694.5
52	95241.5
53	87354.5
54	79143.0
55	71532.0
56	64758.5
57	58430.5
58	52927.5
59	47750.0
60	43026.5
61	38690.5
62	34420.5
63	30413.5
64	26631.0
65	22871.5
66	19360.5
67	16082.0
68	12875.5
69	10097.5
70	7713.0
71	5670.5
72	4090.0
73	2826.0
74	1855.5
75	1190.5
76	772.0
77	469.0
78	247.0
79	128.5
80	83.0
81	44.5
82	20.5
83	14.0
84	9.0
85	2.5
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	324.0
92-93	772.0
94-95	1137.0
96-97	1569.0
98-99	2082.0
100-101	2424.0
102-103	2799.0
104-105	3006.0
106-107	3375.0
108-109	3754.0
110-111	3887.0
112-113	4121.0
114-115	4307.0
116-117	4287.0
118-119	4531.0
120-121	4597.0
122-123	4911.0
124-125	5205.0
126-127	5340.0
128-129	5593.0
130-131	5761.0
132-133	6520.0
134-135	30114.0
136-137	32374.0
138-139	31742.0
140-141	32815.0
142-143	33382.0
144-145	34987.0
146-147	30245.0
148-149	28658.0
150-151	28938.0
152-153	28852.0
154-155	26395.0
156-157	25685.0
158-159	26388.0
160-161	28055.0
162-163	28483.0
164-165	29207.0
166-167	31009.0
168-169	31906.0
170-171	32186.0
172-173	34738.0
174-175	38204.0
176-177	78395.0
178-179	2199335.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.46640053569627
#Duplication Level	Percentage of total
1	95.1138525605704
2	4.433907454386081
3	0.3171778119073857
4	0.07878555509862975
5	0.03631144683796574
6	0.010856812749025365
7	0.00441426585464805
8	0.0011216390166358403
9	0.0022797972223070648
>10	0.0012926563569297053
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.3306743449812566E-5	0.0	0.0	0.0	0.0	0.0
2	3.3306743449812566E-5	0.0	0.0	0.0	0.0	0.0
3	3.3306743449812566E-5	0.0	0.0	0.0	0.0	0.0
4	3.3306743449812566E-5	0.0	0.0	3.3306743449812566E-5	0.0	0.0
5	3.3306743449812566E-5	0.0	3.3306743449812566E-5	3.3306743449812566E-5	0.0	0.0
6	3.3306743449812566E-5	3.3306743449812566E-5	6.661348689962513E-5	3.3306743449812566E-5	0.0	0.0
7	3.3306743449812566E-5	3.3306743449812566E-5	6.661348689962513E-5	3.3306743449812566E-5	0.0	0.0
8	3.3306743449812566E-5	3.3306743449812566E-5	6.661348689962513E-5	3.3306743449812566E-5	0.0	0.0
9	3.3306743449812566E-5	3.3306743449812566E-5	6.661348689962513E-5	3.3306743449812566E-5	0.0	0.0
10-14	5.9952138209662625E-5	3.3306743449812566E-5	6.661348689962513E-5	3.3306743449812566E-5	0.0	0.0
15-19	7.993618427955016E-5	3.3306743449812566E-5	6.661348689962513E-5	3.3306743449812566E-5	0.0	0.0
20-24	9.99202303494377E-5	4.66294408297376E-5	7.327483558958764E-5	5.3290789519700105E-5	0.0	0.0
25-29	9.99202303494377E-5	6.661348689962513E-5	1.0658157903940021E-4	6.661348689962513E-5	0.0	0.0
30-34	1.3322697379925026E-4	6.661348689962513E-5	1.4654967117917528E-4	9.325888165947518E-5	1.998404606988754E-5	0.0
35-39	2.4646990152861297E-4	9.99202303494377E-5	2.3980855283865045E-4	9.99202303494377E-5	3.3306743449812566E-5	0.0
40-44	3.264060858081631E-4	1.3322697379925026E-4	3.3306743449812567E-4	1.1990427641932525E-4	6.661348689962513E-5	2.6645394759850053E-5
45-49	4.063422700877133E-4	1.3322697379925026E-4	3.996809213977508E-4	1.3322697379925026E-4	9.99202303494377E-5	3.3306743449812566E-5
50-54	4.6629440829737596E-4	1.3322697379925026E-4	4.6629440829737596E-4	1.4654967117917528E-4	9.99202303494377E-5	1.1324292772936274E-4
55-59	4.8627845436726347E-4	1.4654967117917528E-4	5.129238491271135E-4	2.2648585545872543E-4	1.3988832248921278E-4	1.5321101986913782E-4
60-64	5.395692438869635E-4	1.998404606988754E-4	5.662146386468136E-4	2.5979259890853806E-4	1.6653371724906284E-4	1.8651776331895037E-4
65-69	5.662146386468136E-4	1.998404606988754E-4	5.662146386468136E-4	2.797766449784255E-4	1.6653371724906284E-4	2.6645394759850047E-4
70-74	8.260072375553516E-4	1.998404606988754E-4	5.995213820966261E-4	3.2640608580816315E-4	1.6653371724906284E-4	2.9976069104831307E-4
75-79	0.006534783064853225	2.0650180938883793E-4	6.594735203062889E-4	3.3972878318808814E-4	1.998404606988754E-4	3.4639013187805066E-4
80-84	0.008892900501099955	2.3314720414868798E-4	6.927802637561013E-4	3.996809213977508E-4	2.398085528386505E-4	3.663741779479382E-4
85-89	0.008939529941929692	2.3314720414868798E-4	7.39409704585839E-4	3.996809213977508E-4	5.129238491271135E-4	3.663741779479382E-4
90-94	0.00895951398799958	2.3314720414868798E-4	8.326685862453142E-4	3.996809213977508E-4	5.662146386468136E-4	3.663741779479382E-4
95-99	0.008972836685379505	2.3314720414868798E-4	8.393299349352768E-4	4.2632631615760084E-4	5.662146386468136E-4	3.663741779479382E-4
100-104	0.009026127474899205	2.5979259890853806E-4	8.792980270750518E-4	4.329876648475633E-4	5.662146386468136E-4	3.663741779479382E-4
105-109	0.00903945017227913	2.7311529628846305E-4	9.325888165947519E-4	5.062625004371511E-4	5.662146386468136E-4	3.663741779479382E-4
110-114	0.009106063659178754	2.9976069104831307E-4	9.658955600445645E-4	5.662146386468136E-4	5.662146386468136E-4	3.663741779479382E-4
115-119	0.009132709053938605	3.064220397382756E-4	9.725569087345269E-4	5.928600334066636E-4	5.995213820966261E-4	3.663741779479382E-4
120-124	0.009205983889528193	3.3306743449812567E-4	9.99202303494377E-4	6.261667768564763E-4	5.995213820966261E-4	3.7969687532786326E-4
125-129	0.009265936027737856	3.3972878318808814E-4	0.0010924611851538522	6.661348689962513E-4	6.328281255464388E-4	3.996809213977508E-4
130-134	0.009385840304157182	3.663741779479382E-4	0.0011390906259835898	7.194256585159514E-4	6.661348689962513E-4	4.2632631615760084E-4
135-139	0.009499083231886543	3.663741779479382E-4	0.0011657360207434399	7.727164480356514E-4	6.661348689962513E-4	4.8627845436726347E-4
140-144	0.00961898750830587	3.863582240178257E-4	0.0011657360207434399	7.993618427955016E-4	6.927802637561013E-4	4.996011517471885E-4
145-149	0.009738891784725196	4.2632631615760084E-4	0.0011923814155032897	8.060231914854641E-4	6.994416124460639E-4	5.32907895197001E-4
150-154	0.009825489317694707	4.329876648475633E-4	0.0012123654615731777	8.326685862453142E-4	6.994416124460639E-4	5.32907895197001E-4
155-159	0.010005345732323695	4.5297171091745087E-4	0.00124567220502299	8.326685862453142E-4	7.194256585159514E-4	5.32907895197001E-4
160-164	0.010085281916603246	4.6629440829737596E-4	0.0012656562510928776	8.326685862453142E-4	7.327483558958764E-4	5.32907895197001E-4
165-167	0.01012525000874302	4.662944082973759E-4	0.0012656562510928776	8.326685862453141E-4	7.327483558958765E-4	5.32907895197001E-4
>>END_MODULE
