##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293817
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5126979
Total Bases	878.8 Mbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.35722069468199	39.0	39.0	39.0	37.0	39.0
2	40.05716114694443	41.0	40.0	41.0	38.0	41.0
3	40.12750725134626	41.0	40.0	41.0	38.0	41.0
4	40.162773438315234	41.0	40.0	41.0	38.0	41.0
5	40.181058280129484	41.0	40.0	41.0	38.0	41.0
6	40.172786742446185	41.0	40.0	41.0	38.0	41.0
7	40.19497290704721	41.0	40.0	41.0	38.0	41.0
8	40.16944149761487	41.0	40.0	41.0	38.0	41.0
9	40.15051105143984	41.0	40.0	41.0	38.0	41.0
10-14	40.09324641275106	41.0	40.0	41.0	38.0	41.0
15-19	39.95524054223745	41.0	40.0	41.0	38.0	41.0
20-24	39.767455025659366	40.8	40.0	41.0	37.6	41.0
25-29	39.50915281689275	40.0	39.0	41.0	36.6	41.0
30-34	39.28181859141612	40.0	38.6	41.0	35.4	41.0
35-39	38.89150690884437	40.0	37.6	41.0	35.0	41.0
40-44	38.325551206665764	39.4	36.4	41.0	35.0	41.0
45-49	37.779259091952596	38.4	35.0	40.6	34.6	41.0
50-54	37.0175236918271	36.8	35.0	39.4	34.0	41.0
55-59	36.258421889381644	35.6	35.0	38.0	34.0	40.4
60-64	35.37245668453099	34.8	34.6	36.4	32.8	38.8
65-69	35.16970687806601	35.0	34.8	36.0	33.4	37.4
70-74	35.1222216825932	35.0	35.0	35.2	33.6	37.0
75-79	35.0080996235795	35.0	35.0	35.0	33.0	36.0
80-84	34.959933871388984	35.0	35.0	35.0	33.0	36.0
85-89	34.84298601574143	35.0	34.8	35.0	32.6	36.2
90-94	39.58848060730881	40.6	39.8	40.6	37.8	40.6
95-99	39.888986826377604	41.0	40.0	41.0	38.0	41.0
100-104	39.771259335449514	41.0	40.0	41.0	37.0	41.0
105-109	39.59771291330643	41.0	39.4	41.0	36.4	41.0
110-114	39.402378743895554	40.4	39.0	41.0	35.4	41.0
115-119	39.11741792453292	40.0	38.0	41.0	35.0	41.0
120-124	38.80840495286664	40.0	37.8	41.0	35.0	41.0
125-129	38.07587879307563	39.4	36.4	40.4	34.4	40.6
130-134	37.954847862702266	39.0	35.4	40.8	34.0	41.0
135-139	37.35614379464713	37.4	35.0	40.2	34.0	41.0
140-144	36.57402686832774	36.2	35.0	39.0	33.6	41.0
145-149	35.93524549558997	35.0	35.0	37.2	33.6	39.4
150-154	35.456403715068014	35.0	35.0	36.2	34.0	38.2
155-159	35.02273270238752	35.0	35.0	35.4	33.8	36.6
160-164	34.7501236234097	35.0	35.0	35.0	33.0	36.0
165-169	34.6058835105931	35.0	35.0	35.0	33.0	36.0
170-174	34.51860062562132	35.0	35.0	35.0	33.0	35.0
175-178	34.28820962495644	35.0	34.25	35.0	32.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
30	116.0
31	1246.0
32	8225.0
33	35717.0
34	115082.0
35	318753.0
36	895923.0
37	1890779.0
38	1686714.0
39	174131.0
40	293.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.68755148012114	26.909374896990997	27.78915224735658	22.61392137553128
2	23.09562804918842	27.659387721307226	27.672728911119005	21.57225531838535
3	20.66601013969435	26.439644086702913	29.842135105293	23.05221066830974
4	21.745281188005645	25.273986883894008	29.99390089173371	22.986831036366638
5	22.914605267546445	26.070225760628237	27.847783265739924	23.167385706085398
6	22.750258973169192	26.539742019618178	27.68246173818929	23.027537269023338
7	22.934617052264112	26.685071267114612	27.123224027248792	23.257087653372484
8	23.176650421232463	26.76738094694751	26.788816572098305	23.267152059721717
9	23.163309231420687	26.876880127654125	26.89431729679408	23.065493344131117
10-14	23.231017720181807	26.937410900259195	26.6269629737122	23.2046084058468
15-19	23.12199055233111	26.917613666839674	26.852304251685055	23.10809152914416
20-24	23.050213390770665	26.74036308711231	26.982493979398004	23.226929542719017
25-29	23.1225951968986	26.72839502560865	26.788215828463507	23.360793949029244
30-34	23.110989922135435	26.813724027346318	26.800831444794294	23.274454605723953
35-39	23.05665383064764	26.84085891516232	26.820094250434806	23.282393003755235
40-44	23.167506634998894	26.79574462856197	26.70414292705314	23.332605809385996
45-49	23.299311348846953	26.704135125187754	26.704341874620514	23.29221165134478
50-54	23.29215703828707	26.784513843337372	26.658131425933284	23.265197692442275
55-59	23.269203950318502	26.697452827483787	26.664657686329512	23.3686855358682
60-64	23.19574548676716	26.771734387833458	26.729140103753107	23.303380021646277
65-69	23.214836651369158	26.752849972664215	26.72377242036685	23.30854095559978
70-74	23.197656943786974	26.758533631598645	26.681400489450024	23.362408935164353
75-79	23.162977652141738	26.757363351790598	26.74058153934315	23.339077456724517
80-84	23.16688638670063	26.790560289012298	26.70116261447531	23.341390709811762
85-89	23.125497490822568	26.756403722347994	26.681685257536653	23.436413529292786
90-94	22.275679265025914	26.564808449642584	28.398681616050325	22.76083066928117
95-99	22.98448740313657	26.80304036933274	26.995234564856396	23.217237662674293
100-104	23.175308289830244	26.97075075628814	26.686776718846378	23.16716423503523
105-109	23.04699139980921	26.926768875651625	26.852553374399555	23.173686350139604
110-114	23.014774828014847	26.769832191013794	26.94685022134039	23.268542759630968
115-119	23.07205840972086	26.851645834386716	26.77661512879376	23.299680627098656
120-124	23.06557931092138	26.858824028168872	26.742223706684953	23.333372954224796
125-129	23.040891120087824	26.861140703992636	26.816698332022476	23.281269843897064
130-134	23.145164785135762	26.874879967352722	26.71015639704249	23.269798850469027
135-139	23.226502371102466	26.80596456777316	26.65480100863115	23.31273205249322
140-144	23.17434955422159	26.914183724427392	26.69762849765089	23.21383822370013
145-149	23.115608326132854	26.97662782758351	26.716185422774885	23.191578423508748
150-154	23.06417305446995	27.013434058942416	26.764511768137805	23.157881118449826
155-159	23.033691629366633	26.989932534586064	26.872588735795055	23.103787100252244
160-164	22.999981759500933	27.126892340982383	26.81556294308726	23.057562956429418
165-169	22.93923094090493	27.104090832751538	26.85308820779928	23.103590018544246
170-174	22.88810100586862	27.18933974519424	26.91313894947905	23.009420299458085
175-178	22.862012687136293	27.247680113682687	26.900619496332865	22.989687702848155
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	1.5
14	1.5
15	8.0
16	17.5
17	32.5
18	71.0
19	167.0
20	339.5
21	600.5
22	1114.0
23	1998.5
24	3295.5
25	5221.5
26	8107.0
27	12037.5
28	17254.5
29	23898.0
30	32551.0
31	43462.0
32	56087.5
33	70740.0
34	87446.0
35	105763.5
36	125194.5
37	144975.5
38	164073.0
39	182521.0
40	199671.5
41	213999.5
42	225195.0
43	232608.5
44	237333.0
45	238325.5
46	234529.0
47	227542.0
48	217137.5
49	205075.0
50	191480.0
51	177132.5
52	163014.0
53	148987.0
54	135797.5
55	122879.0
56	110981.0
57	100764.0
58	91220.5
59	82198.5
60	74004.5
61	66491.5
62	59603.0
63	52850.5
64	46201.5
65	39759.0
66	33788.0
67	27924.5
68	22345.0
69	17497.0
70	13421.5
71	10033.0
72	7232.0
73	4995.5
74	3207.5
75	2044.5
76	1322.5
77	801.5
78	449.0
79	246.0
80	142.0
81	75.5
82	40.5
83	18.0
84	8.5
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	521.0
92-93	1250.0
94-95	1963.0
96-97	2720.0
98-99	3333.0
100-101	4152.0
102-103	4598.0
104-105	5249.0
106-107	5647.0
108-109	6386.0
110-111	6469.0
112-113	6924.0
114-115	7036.0
116-117	7413.0
118-119	7486.0
120-121	7927.0
122-123	8144.0
124-125	8530.0
126-127	8912.0
128-129	9210.0
130-131	10019.0
132-133	11202.0
134-135	51993.0
136-137	55362.0
138-139	54076.0
140-141	55189.0
142-143	56175.0
144-145	58972.0
146-147	50325.0
148-149	47975.0
150-151	47541.0
152-153	47409.0
154-155	43670.0
156-157	43494.0
158-159	45368.0
160-161	46862.0
162-163	48003.0
164-165	49027.0
166-167	51281.0
168-169	53562.0
170-171	55149.0
172-173	58715.0
174-175	61891.0
176-177	127556.0
178-179	3782293.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.8962949484918
#Duplication Level	Percentage of total
1	92.18863245534618
2	6.860722057438175
3	0.6428935593165451
4	0.14403647056493482
5	0.07064378926414638
6	0.03986164615652496
7	0.02541998900455147
8	0.0032082173462283695
9	0.008637674973100721
>10	0.015944140589579218
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.9504663467511763E-5	0.0	1.9504663467511763E-5	1.9504663467511763E-5	0.0	0.0
2	1.9504663467511763E-5	0.0	3.9009326935023527E-5	1.9504663467511763E-5	1.9504663467511763E-5	0.0
3	5.851399040253529E-5	1.9504663467511763E-5	3.9009326935023527E-5	1.9504663467511763E-5	1.9504663467511763E-5	0.0
4	7.801865387004705E-5	1.9504663467511763E-5	3.9009326935023527E-5	1.9504663467511763E-5	1.9504663467511763E-5	0.0
5	7.801865387004705E-5	1.9504663467511763E-5	7.801865387004705E-5	1.9504663467511763E-5	1.9504663467511763E-5	0.0
6	7.801865387004705E-5	1.9504663467511763E-5	1.1702798080507059E-4	1.9504663467511763E-5	1.9504663467511763E-5	0.0
7	7.801865387004705E-5	1.9504663467511763E-5	1.1702798080507059E-4	1.9504663467511763E-5	1.9504663467511763E-5	0.0
8	7.801865387004705E-5	1.9504663467511763E-5	1.1702798080507059E-4	3.9009326935023527E-5	1.9504663467511763E-5	0.0
9	7.801865387004705E-5	3.9009326935023527E-5	1.1702798080507059E-4	3.9009326935023527E-5	1.9504663467511763E-5	0.0
10-14	7.801865387004705E-5	4.681119232202823E-5	1.5213637504659175E-4	3.9009326935023527E-5	1.9504663467511763E-5	0.0
15-19	9.362238464405647E-5	7.411772117654469E-5	1.8724476928811293E-4	3.9009326935023527E-5	1.9504663467511763E-5	0.0
20-24	1.638391731270988E-4	9.362238464405647E-5	2.340559616101412E-4	3.9009326935023527E-5	1.9504663467511763E-5	0.0
25-29	2.145512981426294E-4	9.752331733755883E-5	2.5356062507765295E-4	3.9009326935023527E-5	1.9504663467511763E-5	0.0
30-34	3.003718173996812E-4	1.2482984619207528E-4	2.730652885451647E-4	4.681119232202823E-5	5.8513990402535286E-5	0.0
35-39	4.4860725975277057E-4	1.560373077400941E-4	3.120746154801882E-4	7.021678848304235E-5	9.362238464405647E-5	1.1702798080507058E-5
40-44	5.500315097838318E-4	1.560373077400941E-4	3.120746154801882E-4	1.1312704811156824E-4	9.752331733755883E-5	9.362238464405647E-5
45-49	6.436538944278883E-4	1.638391731270988E-4	3.1987648086719293E-4	1.404335769660847E-4	1.170279808050706E-4	1.2873077888557764E-4
50-54	6.982669521369211E-4	1.7554197120760588E-4	3.315792789477E-4	1.9504663467511765E-4	1.170279808050706E-4	1.911457019816153E-4
55-59	7.099697502174283E-4	1.9504663467511765E-4	3.4328207702820704E-4	2.301550289166388E-4	1.4433450965958703E-4	2.691643558516624E-4
60-64	7.723846733134658E-4	1.9504663467511765E-4	4.408053943657659E-4	2.5356062507765295E-4	1.560373077400941E-4	3.3157927894770004E-4
65-69	7.996912021679825E-4	2.0674943275562468E-4	4.7591378860728707E-4	2.6916435585166233E-4	1.911457019816153E-4	3.7058860588272353E-4
70-74	0.001115666750341673	2.145512981426294E-4	5.032203174618036E-4	3.3157927894769993E-4	2.184522308361318E-4	4.408053943657659E-4
75-79	0.00664718930972801	2.3795689430364353E-4	5.344277790098224E-4	3.5108394241521176E-4	2.5356062507765295E-4	5.071212501553059E-4
80-84	0.00898774892582942	2.5356062507765295E-4	5.656352405578412E-4	3.7058860588272353E-4	3.2767834625419765E-4	5.266259136228177E-4
85-89	0.008991649858522924	2.730652885451647E-4	5.85139904025353E-4	3.939942020437377E-4	5.227249809293153E-4	5.266259136228177E-4
90-94	0.008999451723909929	2.8086715393216944E-4	6.475548271213905E-4	4.095979328177471E-4	5.461305770903294E-4	5.266259136228177E-4
95-99	0.009030659185457948	3.1207461548018827E-4	6.74861355975907E-4	4.2130073089825413E-4	5.461305770903294E-4	5.266259136228177E-4
100-104	0.009054064781618961	3.4718300972170937E-4	6.826632213629117E-4	4.6421099052678E-4	5.461305770903294E-4	5.266259136228177E-4
105-109	0.009096975041247487	3.939942020437377E-4	6.904650867499164E-4	5.032203174618036E-4	5.617343078643388E-4	5.344277790098222E-4
110-114	0.00913598436818251	4.369044616722636E-4	7.021678848304235E-4	5.071212501553059E-4	5.656352405578412E-4	5.461305770903294E-4
115-119	0.009155489031650022	4.4860725975277057E-4	7.372762790719448E-4	5.071212501553059E-4	5.656352405578412E-4	5.461305770903294E-4
120-124	0.009229606752826566	4.4860725975277057E-4	7.918893367809777E-4	5.227249809293153E-4	5.695361732513435E-4	5.461305770903294E-4
125-129	0.009346634733631638	4.4860725975277057E-4	8.347995964095035E-4	5.773380386383482E-4	6.163473655733718E-4	5.461305770903294E-4
130-134	0.009448058983662699	4.4860725975277057E-4	8.738089233445271E-4	6.241492309603765E-4	6.553566925083953E-4	5.773380386383482E-4
135-139	0.009580690695241778	4.4860725975277057E-4	9.245210483600577E-4	6.553566925083953E-4	6.670594905889024E-4	5.85139904025353E-4
140-144	0.009686015877966343	4.681119232202824E-4	0.0010103415676171095	6.631585578954E-4	7.021678848304235E-4	5.85139904025353E-4
145-149	0.00983035038762593	4.915175193812965E-4	0.001072756490713147	6.670594905889024E-4	7.216725482979353E-4	5.85139904025353E-4
150-154	0.009881062512641462	5.149231155423107E-4	0.0010766574234066493	6.865641540564141E-4	7.684837406199635E-4	5.85139904025353E-4
155-159	0.009974684897285516	5.344277790098222E-4	0.0011078648849546682	7.021678848304235E-4	7.801865387004706E-4	5.85139904025353E-4
160-164	0.01002929795499455	5.539324424773341E-4	0.0011312704811156824	7.255734809914376E-4	7.801865387004706E-4	5.85139904025353E-4
165-167	0.010142425003106118	5.656352405578412E-4	0.0011312704811156824	7.671834297221294E-4	7.996912021679824E-4	6.501554489170588E-4
>>END_MODULE
