##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1293838
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9243220
Total Bases	1.5 Gbp
Sequences flagged as poor quality	0
Sequence length	90-178
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.40465108479513	39.0	39.0	39.0	37.0	39.0
2	40.23414794844221	41.0	40.0	41.0	38.0	41.0
3	40.275219133591975	41.0	40.0	41.0	39.0	41.0
4	40.29086519632769	41.0	40.0	41.0	39.0	41.0
5	40.2848394823449	41.0	40.0	41.0	39.0	41.0
6	40.28472177444657	41.0	40.0	41.0	39.0	41.0
7	40.301485954029005	41.0	40.0	41.0	39.0	41.0
8	40.280101955811936	41.0	40.0	41.0	39.0	41.0
9	40.2895364386004	41.0	40.0	41.0	39.0	41.0
10-14	40.22033427744877	41.0	40.0	41.0	38.4	41.0
15-19	40.13742440404967	41.0	40.0	41.0	38.0	41.0
20-24	39.997305635914756	41.0	40.0	41.0	38.0	41.0
25-29	39.797185309881186	41.0	40.0	41.0	37.4	41.0
30-34	39.48698925266304	40.4	39.0	41.0	36.0	41.0
35-39	39.02727393700464	40.0	37.8	41.0	35.0	41.0
40-44	38.52325135612914	39.8	36.6	41.0	35.0	41.0
45-49	37.97942831610629	39.0	35.2	41.0	35.0	41.0
50-54	37.41064793437785	37.2	35.0	40.4	35.0	41.0
55-59	36.70500128743014	35.8	35.0	39.0	34.6	41.0
60-64	35.926507559054095	35.0	34.8	37.0	33.6	40.0
65-69	35.54520755753947	35.0	35.0	36.0	33.8	39.0
70-74	35.3358603819881	35.0	35.0	35.8	34.0	37.2
75-79	35.16023980820536	35.0	35.0	35.0	34.0	36.6
80-84	35.08023171578736	35.0	35.0	35.0	34.0	36.0
85-89	34.96325088010455	35.0	34.8	35.0	33.8	36.0
90-94	39.68457384988622	40.6	39.8	40.6	37.8	40.6
95-99	40.01005408791958	41.0	40.0	41.0	38.0	41.0
100-104	39.90239294547929	41.0	40.0	41.0	37.8	41.0
105-109	39.77534215214085	41.0	40.0	41.0	37.0	41.0
110-114	39.602949665630845	41.0	39.4	41.0	36.2	41.0
115-119	39.34495717193191	40.2	38.8	41.0	35.2	41.0
120-124	39.000802800763545	40.0	38.0	41.0	35.0	41.0
125-129	38.09833577965197	39.4	36.4	40.4	34.4	40.6
130-134	38.06014094594103	39.0	35.6	40.8	34.6	41.0
135-139	37.75578543378076	38.4	35.0	41.0	35.0	41.0
140-144	37.12154157558159	36.6	35.0	40.0	34.4	41.0
145-149	36.42257779043679	35.4	35.0	38.4	34.0	41.0
150-154	35.808001472661026	35.0	35.0	36.8	34.0	39.2
155-159	35.32240148972489	35.0	35.0	36.0	34.0	37.6
160-164	34.9886478382438	35.0	35.0	35.0	34.0	36.4
165-169	34.80047730019267	35.0	35.0	35.0	34.0	36.0
170-174	34.695691313828284	35.0	35.0	35.0	33.4	36.0
175-178	34.539065472858056	35.0	34.75	35.0	32.75	35.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
28	2.0
29	59.0
30	495.0
31	3425.0
32	16902.0
33	60180.0
34	166178.0
35	431124.0
36	1312134.0
37	3087796.0
38	3280071.0
39	871166.0
40	13688.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.590103881547773	26.89755301723858	27.265552480629047	23.2467906205846
2	23.711866643875187	27.22251553030221	26.994391564844282	22.071226260978317
3	21.89789921693955	26.51208128768979	27.932787491804806	23.657232003565856
4	22.46704070659359	25.619416177479277	28.325064209225793	23.588478906701344
5	23.25316285883058	26.17683015226296	26.999811753912596	23.570195234993864
6	23.154733956348544	26.29275295838463	27.007265866224106	23.545247219042714
7	23.286841598490568	26.407647984144056	26.646114665668456	23.65939575169692
8	23.410965010028974	26.610066621804958	26.526902962387567	23.452065405778505
9	23.37293713662555	26.667103022539763	26.601952566313468	23.358007274521217
10-14	23.410140621991037	26.601578237886798	26.442611990193893	23.545669149928273
15-19	23.344912270832026	26.59217675225733	26.562628607779544	23.500282369131103
20-24	23.341156004076502	26.540800716633385	26.614199380735286	23.503843898554834
25-29	23.348426197796872	26.5438948764608	26.487622278816257	23.62005664692607
30-34	23.297316303193043	26.598499224296297	26.568747687494188	23.53543678501648
35-39	23.272779399386796	26.62357490138718	26.548642139860352	23.555003559365677
40-44	23.308396857372216	26.620141033103184	26.50367296245248	23.567789147072123
45-49	23.469498724470476	26.524561786909757	26.42003976969065	23.585899718929117
50-54	23.41607145561828	26.612325574853784	26.471530483965545	23.50007248556239
55-59	23.41925000162281	26.54597423841475	26.43239693526715	23.602378824695293
60-64	23.3732054413938	26.589978384156172	26.482199925999815	23.554616248450216
65-69	23.38363470738552	26.597503900156006	26.468789015083487	23.550072377374985
70-74	23.339509391748763	26.616800205988824	26.466015089979468	23.57767531228295
75-79	23.33575528874137	26.60153063542791	26.497705345107008	23.565008730723708
80-84	23.321632504689923	26.640201142026264	26.481124543178673	23.557041810105137
85-89	23.312864997262857	26.63913225044952	26.426745225148814	23.621257527138813
90-94	22.733653304369543	26.528474679745845	27.47600552644457	23.26186648944004
95-99	23.195598123672998	26.57902425115169	26.686456428540605	23.538921196634703
100-104	23.268510678491914	26.676996139528036	26.552517083467336	23.501976098512714
105-109	23.215366386935877	26.65862441457822	26.641144812566157	23.484864385919746
110-114	23.23299473661754	26.54915141185188	26.676905334489025	23.540948517041546
115-119	23.248812680567397	26.625118305831936	26.574996279971835	23.551072733628832
120-124	23.205331452673246	26.621823857594666	26.5969575949419	23.575887094790186
125-129	23.177048680473185	26.62884394313506	26.60704482093849	23.587062555453258
130-134	23.326311840233384	26.630924003893735	26.5534330168414	23.48933113903148
135-139	23.42461196213643	26.559812001603177	26.47846774561821	23.537108290642173
140-144	23.367895762030663	26.647895115514704	26.56414336875977	23.420065753694868
145-149	23.365191012443457	26.668570794107886	26.544844709756305	23.421393483692352
150-154	23.29989502500498	26.724645538109975	26.57650390810364	23.398955528781404
155-159	23.26271693147807	26.73663544098637	26.625803548490662	23.3748440790449
160-164	23.2029440633977	26.87071749436882	26.659597971035858	23.266740471197622
165-169	23.15444036430419	26.85135185798016	26.68242652731432	23.311781250401335
170-174	23.116968917410226	26.92345353679293	26.73805898060937	23.221518565187473
175-178	23.09392432058237	26.991250611541528	26.706374218864525	23.208450849011577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	1.0
9	2.0
10	4.5
11	11.5
12	19.0
13	35.0
14	74.5
15	153.0
16	298.0
17	567.5
18	1126.5
19	2083.5
20	3519.5
21	5699.0
22	8850.5
23	13139.5
24	18804.0
25	26044.5
26	34895.0
27	45384.5
28	57828.5
29	71083.0
30	85757.5
31	102614.0
32	120060.0
33	139027.5
34	159644.0
35	181855.5
36	205111.0
37	228709.0
38	253104.5
39	276789.0
40	299518.0
41	320715.5
42	338366.0
43	352625.5
44	362917.5
45	367864.0
46	368962.5
47	365259.0
48	357299.5
49	346813.5
50	333712.5
51	319159.5
52	303806.0
53	286624.0
54	269230.0
55	252631.5
56	236071.5
57	220076.5
58	203966.5
59	187266.5
60	170737.5
61	154624.5
62	137804.5
63	121324.5
64	105428.5
65	89971.5
66	75489.5
67	61981.0
68	49960.5
69	39337.5
70	30092.0
71	22438.0
72	16325.5
73	11617.5
74	7863.0
75	5144.5
76	3355.5
77	2137.0
78	1275.0
79	736.5
80	421.5
81	234.0
82	114.5
83	59.0
84	35.0
85	18.0
86	7.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-178	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
90-91	758.0
92-93	1595.0
94-95	2406.0
96-97	3036.0
98-99	3516.0
100-101	4218.0
102-103	4663.0
104-105	5640.0
106-107	6312.0
108-109	6800.0
110-111	7380.0
112-113	7560.0
114-115	8323.0
116-117	9829.0
118-119	11468.0
120-121	14959.0
122-123	10164.0
124-125	9985.0
126-127	11779.0
128-129	11167.0
130-131	11379.0
132-133	12243.0
134-135	90349.0
136-137	91174.0
138-139	80406.0
140-141	76618.0
142-143	86137.0
144-145	76489.0
146-147	79736.0
148-149	116983.0
150-151	72935.0
152-153	79725.0
154-155	78150.0
156-157	86933.0
158-159	80993.0
160-161	75705.0
162-163	80479.0
164-165	72725.0
166-167	84720.0
168-169	78597.0
170-171	77858.0
172-173	86786.0
174-175	83143.0
176-177	158244.0
178-179	7183155.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.03182237749354
#Duplication Level	Percentage of total
1	90.6118942150718
2	7.983337091976768
3	1.0507006529483567
4	0.2173753215773549
5	0.07353184557327898
6	0.030803951818700402
7	0.010323408111540146
8	0.011416931696786863
9	0.0031304538047290517
>10	0.007486127420708682
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.353809603146955E-4	0.0	0.0	2.1637481310625517E-5	0.0	0.0
2	0.0012982488786375311	1.0818740655312759E-5	0.0	2.1637481310625517E-5	0.0	0.0
3	0.0012982488786375311	4.3274962621251034E-5	1.0818740655312759E-5	3.2456221965938274E-5	0.0	0.0
4	0.0012982488786375311	4.3274962621251034E-5	1.0818740655312759E-5	4.3274962621251034E-5	0.0	0.0
5	0.001309067619292844	4.3274962621251034E-5	2.1637481310625517E-5	5.4093703276563794E-5	0.0	0.0
6	0.001309067619292844	7.573118458718931E-5	3.2456221965938274E-5	6.491244393187655E-5	0.0	0.0
7	0.001309067619292844	8.654992524250207E-5	3.2456221965938274E-5	6.491244393187655E-5	2.1637481310625517E-5	0.0
8	0.001309067619292844	9.736866589781483E-5	6.491244393187655E-5	6.491244393187655E-5	2.1637481310625517E-5	0.0
9	0.001309067619292844	9.736866589781483E-5	6.491244393187655E-5	6.491244393187655E-5	2.1637481310625517E-5	0.0
10-14	0.0013177226118170939	1.298248878637531E-4	8.654992524250207E-5	7.573118458718931E-5	2.1637481310625517E-5	0.0
15-19	0.0013307051006034693	1.8175484300925437E-4	9.953241402887738E-5	1.0602365842206504E-4	2.380122944168807E-5	0.0
20-24	0.0013501788337830323	2.07719820582005E-4	1.1467865094631523E-4	1.341523841258782E-4	4.543871075231358E-5	0.0
25-29	0.0013653250707004701	2.3368479815475562E-4	1.5578986543650372E-4	1.795910948781918E-4	7.14036883250642E-5	0.0
30-34	0.0014691849809914726	2.553222794653811E-4	1.7742734674712924E-4	2.2286605749944287E-4	8.871367337356462E-5	0.0
35-39	0.0018694783852380447	2.8128725703813174E-4	2.0555607245094243E-4	2.791235089070692E-4	1.0385991029100247E-4	1.2982488786375309E-5
40-44	0.002163748131062552	3.050884864798198E-4	2.2502980563050538E-4	3.072522346108823E-4	1.6444485796075393E-4	5.625745140762634E-5
45-49	0.0024926378469840595	3.6134593788744615E-4	2.4883103507219344E-4	3.3970845657682066E-4	2.0771982058200494E-4	7.356743645612677E-5
50-54	0.002786907592808567	4.1327589303294745E-4	2.639772719896313E-4	4.2842212995038517E-4	2.3584854628581816E-4	1.2116989533950291E-4
55-59	0.002979481176473134	4.6087835191632357E-4	2.8994224956238195E-4	4.82515833226949E-4	2.6181352385856873E-4	1.6877235422287903E-4
60-64	0.0030941598274194493	4.8684332948907414E-4	3.505271972321334E-4	5.06317062668637E-4	2.921059976934445E-4	1.8175484300925437E-4
65-69	0.0032759146704287034	4.954983220133242E-4	4.0029340424657206E-4	5.257907958482E-4	3.1374347900407003E-4	1.9040983553350455E-4
70-74	0.005180013025763749	5.604107659452009E-4	4.6087835191632357E-4	5.64738262207326E-4	3.418722047078832E-4	2.1421106497519264E-4
75-79	0.040827763485019285	6.058494766975145E-4	4.933345738822618E-4	6.274869580081401E-4	3.981296561155095E-4	2.4233979067900577E-4
80-84	0.05590259671413209	6.534519355808906E-4	5.019895664065121E-4	6.642706762362034E-4	4.911708257511992E-4	2.683047682517564E-4
85-89	0.05603891284638903	6.70761920629391E-4	5.106445589307622E-4	6.945631500710791E-4	7.33510616430205E-4	2.70468516382819E-4
90-94	0.05622499518566041	6.923994019400167E-4	5.344457883724503E-4	7.183643795127672E-4	7.464931052165803E-4	2.70468516382819E-4
95-99	0.05632885509595141	7.52984349609768E-4	5.40937032765638E-4	7.702943346582685E-4	7.464931052165803E-4	2.70468516382819E-4
100-104	0.05637862130296585	8.3087928232802E-4	5.517557734209508E-4	8.200605416727071E-4	7.464931052165803E-4	2.70468516382819E-4
105-109	0.05645218873942197	9.066104669152092E-4	5.820482472558265E-4	8.763179930803335E-4	7.464931052165803E-4	2.70468516382819E-4
110-114	0.0565430661609266	9.325754444879598E-4	6.145044692217646E-4	9.304116963568972E-4	7.464931052165803E-4	2.70468516382819E-4
115-119	0.05661663359738274	9.671954145849607E-4	6.51288187449828E-4	9.671954145849607E-4	7.464931052165803E-4	2.791235089070692E-4
120-124	0.056908739595076176	0.0010277803622547122	6.815806612847039E-4	0.0010147978734683367	7.681305865272059E-4	2.921059976934445E-4
125-129	0.05736961794699249	0.0010753828211380882	7.140368832506421E-4	0.00104292659917215	7.940955640999565E-4	2.921059976934445E-4
130-134	0.05798412241621427	0.001110002791235089	7.226918757748922E-4	0.0010797103174002133	8.092418010173944E-4	2.921059976934445E-4
135-139	0.058568334411601156	0.0011316402725457145	7.443293570855178E-4	0.001125149028152527	8.460255192454576E-4	3.0076099021769464E-4
140-144	0.05908763396305616	0.0011814064795601534	7.767855790514561E-4	0.001125149028152527	8.633355042939582E-4	3.1374347900407003E-4
145-149	0.05934728373878366	0.0012095352052639663	8.005868084931441E-4	0.0011381315169389024	8.763179930803335E-4	3.3105346405257045E-4
150-154	0.059526874833661855	0.0012398276790988423	8.027505566242067E-4	0.0011619327463805904	8.979554743909591E-4	3.5269094536319594E-4
155-159	0.05979085210565149	0.0012787751454579682	8.222242898037697E-4	0.0011943889683465286	9.195929557015845E-4	3.76492174804884E-4
160-164	0.06005915687390325	0.0013004126267685938	8.481892673765203E-4	0.0012268451903124668	9.21756703832647E-4	3.7865592293594657E-4
165-167	0.06048757900385363	0.001312673866177948	8.546805117697079E-4	0.0012477614222460717	9.340179432420015E-4	3.8226216982105085E-4
>>END_MODULE
